Egfr protein tyrosine phosphatases. Medical search. Frequent questions

Autocatalytic activation of Epidermal Growth Factor Receptor (EGFR) at the plasma membrane increases the sensitivity of the cell to extracellular growth factors but can also generate spontaneous receptor activation in the absence of stimulation. (tu-dortmund.de)
Epidermal progress issue receptor (EGFR) has been urged to regulate CSPG performance. (anaheimpoliceassociation.org)
Persistent signaling from the oncogenic epidermal development element receptor (EGFR) is certainly a major way to obtain cancers resistance to EGFR targeting. (bioinf.org)
VacA can bind to a number of epithelial cell receptors, such as the receptor protein tyrosine phosphatase (RPTP), the epidermal growth factor receptor (EGFR), and glycosylphosphatidylinositol (GPI) - anchored protein. (medscape.com)
Rapid Evaluation of Tyrosine Kinase Activity of Membrane Integrated Human Epidermal Growth Factor Receptor Using the Yeast Gγ Recruitment System, ACS Synth Biol. (adooq.cn)
Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) have been emerged as the standard selection in non-small cell lung cancer (NSCLC) patients with EGFR sensitive mutations. (biomedcentral.com)
Owing to the well-established beneficial outcomes of clinical trials, epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs), have been approved as the standard regimens in patients with non-small cell lung cancer (NSCLC) harboring EGFR sensitive mutations [ 1 ]. (biomedcentral.com)
The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein that mediates the response of cells to EGF and transforming growth factor α. (cancer-research-network.com)

In recent years, the search for intracellular signaling integrator downstream of receptor tyrosine kinases provided valuable novel substrates. (springer.com)
The role of protein tyrosine kinases (PTKs) has been widely studied but less is known on the protein tyrosine phosphatases (PTPs) responsible for immunoregulation [ 3 ]. (biomedcentral.com)
[ 5 ] When CagA is translocated to epithelial cells, it induces cell-signaling pathways, following phosphorylation of tyrosine residues by host cell Scr kinases. (medscape.com)
Gab1 tyrosine phosphorylation is sensitive to inhibition by the Src inhibitor dasatinib in GOF SHP2-mutantexpressing cells, suggesting that Src loved ones kinases are involved in SHP2 mutant-induced Gab1 tyrosine phosphorylation.Introduction Because protein tyrosine phosphatases (PTPs) counter the biochemical reaction of protein tyrosine kinases (PTKs) and a lot of PTKs have oncogenic activity, PTPs have been mainly perceived as tumor suppressors. (squalene-epoxidase.com)
Salinomycin co-treatment enhances tamoxifen cytotoxicity in luminal A breast tumor cells by facilitating lysosomal degradation of receptor tyrosine kinases. (mpg.de)
Dual specificity phosphatase 3 (DUSP3), a suppressor of multiple protein tyrosine (Tyr) kinases, is decreased in lung cancer tissues. (biomedcentral.com)
DUSP3/VHR, related to the vaccinia virus phosphatase VH1, is initially found to be a phosphatase that dephosphorylates multiple protein tyrosine kinases (PTKs) in vitro [ 21 ]. (biomedcentral.com)
Levels of cellular protein phosphorylation are modulated both by protein kinases and phosphatases. (rupress.org)
Although the importance of kinases in this process has long been recognized, an appreciation for the complex and fundamental role of phosphatases is more recent. (rupress.org)
Through extensive biochemical and genetic analysis, we now know that pathways are not simply switched on with kinases and off with phosphatases. (rupress.org)
Furthermore, kinases and phosphatases may work together to modulate the strength of a signal. (rupress.org)
Adding further complexity to this picture is the fact that both kinases and phosphatases can function in signaling networks where multiple kinases and phosphatases contribute to the outcome of a pathway. (rupress.org)
To fully understand this complex and essential regulatory process, the kinases and phosphatases mediating the changes in cellular phosphorylation must be identified and characterized. (rupress.org)
A variety of approaches, including biochemical purification, gene isolation by homology, and genetic screens, have been successfully used for the identification of putative protein kinases and phosphatases. (rupress.org)
Focal adhesion kinase (FAK) is a member of a family of non-receptor protein-tyrosine kinases. (cancer-research-network.com)
MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. (icr.ac.uk)
published on the web 30 OctoberINTRODUCTION The important mechanisms that cause a constitutive activation of the PI3KAkt pathway are mutations and overexpression of upstream receptor tyrosine kinases which include erbB family members members, activating mutations of PIK3CA or RAS along with the loss of tumor suppressor protein phosphatase and tensin homolog (PTEN).1 Akt, also referred to as protein kinase B (PKB), consists of three isoforms: PKBAkt1, PKBAkt2 and PKBAkt3. (bet-bromodomain.com)

Among them are the Sprouty proteins which mainly act as inhibitors of growth factor-dependent neuronal and glial signaling pathways. (springer.com)
Specificity and mechanism of action of some commonly used protein kinase inhibitors. (rndsystems.com)
Nevertheless supplementation of press with cholesterol or an upstream metabolite in the pathway such as for example lanosterol didn't have any influence on viability or level of sensitivity to EGFR inhibitors (Fig. S3D-G) recommending specific results at the amount of the C4 demethylation complicated. (bioinf.org)
Network modeling suggests a job for SC4MOL and NSDHL in trafficking of EGFR No earlier studies have recommended a system HMN-214 for the way the SC4MOL protein might influence sensitization to EGFR inhibitors. (bioinf.org)

An important consequence of reversible phosphorylation of tyrosine residues on proteins is the creation of binding sites for phosphotyrosine recognizing domains such as Src homology 2 (SH2) and phosphotyrosine binding (PTB) domains [ 1 , 2 ]. (biomedcentral.com)
The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues represents a fundamental strategy used by eukaryotic organisms to regulate a host of biological functions, including DNA replication, cell cycle progression, energy metabolism, and cell growth and differentiation. (rupress.org)
DUSP-MKPs dephosphorylate and inactivate the MAPKs extracellular signal-regulated kinase (ERK), JNK/SAPK, and p38 on tyrosine and threonine residues. (cancer-research-network.com)

Receptor dimerization and autophosphorylation attracts proteins containing Src homology 2 (SH2) or phosphotyrosine binding (PTB) domains including adaptor proteins like FRS2 and GRB2. (springer.com)
We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells. (biomedcentral.com)
SRC homology 2 (SH2)-containing tyrosine phosphatase-2 (SHP-2) has a controversial effect on lymphocyte signaling. (biomedcentral.com)
Another negative regulator of hematopoietic cell development and function is SHP-1 (SRC homology 2 (SH2)-containing tyrosine phosphatase 1), that is mainly expressed in hematopoietic and lymphoid cells [ 8 ]. (biomedcentral.com)
Among all sterol metabolizing enzymes and their corresponding substrates ERG1 ERG7 ERG11 ERG24 ERG25 ERG26 ERG27 were conserved between Saccharomyces cerevisiae and humans such that proteins with high levels of sequence homology performed comparable functions in sterol biosynthesis HMN-214 (Fig. 1 S4A S4B and Table S1). (bioinf.org)
The majority of ERG genes downstream of zymosterol (ERG6 ERG2 ERG3 ERG5 and ERG4) showed little or no sequence homology with human genes (KEGG pathways (17)) HMN-214 but instead proteins with unrelated sequence performed comparable enzymatic activities. (bioinf.org)
Recombinant proteins including the catalytic domains of proteins tyrosine phosphatase 1B (PTP1B), T cell proteins tyrosine phosphatase (TCPTP), Src homology 848942-61-0 manufacture 2 (SH2) domain-containing tyrosine phosphatase 1 (SHP1), Src homology 2 (SH2) domain-containing tyrosine phosphatase 2 (SHP2), and hematopoietic proteins tyrosine phosphatase (HePTP) had been purified as previously referred to [15C18]. (gasyblog.com)

Noonan syndrome and related disorders are caused by mutations in genes encoding for proteins of the RAS-ERK1/2 signaling pathway, which affect development by enhanced ERK1/2 activity. (sdbonline.org)
Furmonertinib is being advanced by ArriVent in global studies in patients with advanced or metastatic NSCLC with EGFR or HER2 mutations, including exon 20 insertion mutations. (lifecarenews.in)
It is approved in China as a first-line treatment for adults with locally advanced or metastatic NSCLC with EGFR exon 19 deletion (19DEL) or exon 21 (L858R) substitution mutations, where it is being further developed for additional indications with Allist Pharmaceuticals who discovered furmonertinib. (lifecarenews.in)

Identification of a novel inhibitor of mitogen-activated protein kinase kinase. (rndsystems.com)

Second, immunoassays cannot distinctively detect a protein if a high specificity probe is definitely unavailable. (irjs.info)
For decades, researchers have resolved single-stage immunoassay specificity limitations by prepending an upstream polyacrylamide gel electrophoresis (PAGE) protein separation to a downstream immunoassay, therefore developing a two-stage assay known as western blotting. (irjs.info)
Unexpectedly, we find that excitotoxicity provokes an early inactivation of PKD1 through a dephosphorylation-dependent mechanism mediated by protein phosphatase-1 (PP1) and dual specificity phosphatase-1 (DUSP1). (nature.com)
Mitogen-activated protein kinase phosphatases (MKPs) belongs to the dual-specificity phosphatase (DUSP) family. (cancer-research-network.com)

2016) . A cross-species study of PI3K protein-protein interactions reveals the direct interaction of P85 and SHP2 . (sdbonline.org)
These data revealed an unexpected direct binding of Corkscrew, the Drosophila ortholog of the non-receptor protein tyrosine phosphatase type II (SHP2) to the Pi3k21B (p60) regulatory subunit of PI3K (p50/p85 human ortholog) but no association with Pi3k92e , the human ortholog of the p110 catalytic subunit. (sdbonline.org)
The p85-SHP2 association was validated in human cell lines, and formed a ternary regulatory complex with GRB2-associated-binding protein 2 (human GAB2 but not Drosophila Dos ). (sdbonline.org)
Validation experiments with knockdown of GAB2 and Far-Western blots proved the direct interaction of SHP2 with p85, independent of adaptor proteins and transfected FLAG-p85 provided evidence that SHP2 binding on p85 occurred on the SH2 domains. (sdbonline.org)
This study identified that the protein most commonly affected in Noonan syndrome, the phosphatase SHP2, known in Drosophila as corkscrew (CSW) , controls life span, triglyceride levels, and metabolism without affecting ERK signaling pathway. (sdbonline.org)
Noonan syndrome (NS) and NS with multiple lentigines (NSML) cognitive dysfunction are linked to SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) gain-of-function (GoF) and loss-of-function (LoF), respectively. (sdbonline.org)
We generated transgenic mice containing a doxycycline (Dox)-inducible activating SHP2 mutant (tetO-SHP2E76K) and analyzed the function of SHP2E76K in lung tumorigenesis in the Clara cell secretory protein (CCSP)-reverse tetracycline transactivator (rtTA)/tetO-SHP2E76K bitransgenic mice. (squalene-epoxidase.com)
Elevated pGab1 was observed within the lung of Dox-induced CCSP-rtTA/tetO-SHP2E76K mice and in cell lines expressing SHP2E76K, indicating that the activating SHP2 mutant autoregulates tyrosine PDGFR Species phosphorylation of its own docking protein. (squalene-epoxidase.com)

Mechanistically, PRL-3-induced activation of EGFR was attributed primarily to transcriptional downregulation of protein tyrosine phosphatase 1B (PTP1B), an inhibitory phosphatase for EGFR. (nus.edu.sg)
Proteins tyrosine phosphatase 1B (PTP1B) can be an established therapeutic focus on for type 2 diabetes mellitus (T2DM) and weight problems. (gasyblog.com)

Although the role of few PTPs in regulating EGFR phosphorylation is known, it is unclear how PTPs that are spatially segregated in distinct cellular compartments, modulate EGFR autocatalytic activation and hence its downstream signaling. (tu-dortmund.de)
Using single cell measurements of phosphorylation of the EGFR downstream signaling tyrosine residue Y1068, we generated a spatial-temporal reactivity map to identify local phosphatase activity. (tu-dortmund.de)
Here, we provide evidence for PRL-3-induced hyperactivation of EGFR and its downstream signaling cascades in multiple human cancer cell lines. (nus.edu.sg)
The adverse effect of build up of the substrate could be rescued with a upstream blockade while gross adjustments in the great quantity of even more distal upstream or downstream sterols (lanosterol cholesterol) by itself are not adequate to describe the observed results on EGFR. (bioinf.org)
pathway inhibition is carried out upstream, on the EGFR receptor, or downstream, most frequently on the BRAF/MEK/ERK cascade. (oaepublish.com)

SHP-2 can hamper the TRIF (TIR-domain-containing adapter-inducing interferon-β) adaptor protein-dependent TLR4 and TLR3 signal transduction with a consequent block of the pro-inflammatory cytokine production [ 7 ]. (biomedcentral.com)
The RAS gene family, responsible for signal transduction within the mitogen activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PI3K) pathways, is frequently involved in carcinogenesis, and alterations in its member genes can be detected, with variable frequency, in a wide variety of solid and hematological cancers. (oaepublish.com)
These genes encode for closely related GTPase proteins responsible for signal transduction within two main pathways, the mitogen-activated protein kinase (MAPK) and phosphoinositide-3 kinase pathways, involved in the regulation of cell growth, survival, and differentiation. (oaepublish.com)

As a mechanism to control EGFR phosphorylation at the plasma membrane, receptor endocytosis and vesicular trafficking relocalizes activated, phosphorylated EGFR to perinuclear compartments rich in Protein Tyrosine Phosphatases (PTPs), such as PTPN1, which dephosphorylate and inactivate the receptor. (tu-dortmund.de)
Through quantitative imaging of EGFR phosphorylation upon genetic perturbations of classical PTPs and EGFR-PTP interactions, we identified endoplasmic reticulum (ER) associated PTPN2 and plasma membrane associated receptor-like PTPRG/J as strong, direct negative regulators of EGFR. (tu-dortmund.de)
Altogether our findings indicate that by spatially segregating PTPs with different functional relationships to EGFR, the cell is able to sense and respond to its environment. (tu-dortmund.de)

Here, we report a NSCLC patient with brain metastasis, accompanied with EGFR exon 19 deletion, as well as an emerged EGFR Leu792H mutation after the management of AZD9291, who responded positively to the repeating treatment of icotinib, has obtained a 36 months survival during so far. (biomedcentral.com)

Of clinical significance, we verified elevated PRL-3 expression as a predictive marker for favorable therapeutic response in a heterogeneous colorectal cancer (CRC) patient cohort treated with the clinically approved anti-EGFR antibody cetuximab. (nus.edu.sg)
Inactivation of SC4MOL sensitized A431 xenografts to cetuximab a therapeutic anti-EGFR antibody markedly. (bioinf.org)

KRAS is regulated by tyrosine kinase receptors and, in turn, activates the MAPK signaling pathway that is mainly involved in determining cell survival, leading to a strong promotion of cell growth and replication. (oaepublish.com)
In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. (icr.ac.uk)

For this we used the yeast sterol pathway proteins as seeds to mine data from large-scale yeast genetic arrays (18) affinity purification and mass spectroscopy resolution of protein complexes (19-21) and protein complementation screens (22) to gain further insight into their function (Physique S4 Table S1 and supplemental Cytoscape file). (bioinf.org)
The network generated for ERG25 ERG26 ERG27 ERG28 proteins (Fig. 3A S4) revealed as expected many interactions reflecting their participation in the linear ergosterol biosynthesis pathway (green circles in Fig. 3A) as well as additional interactions with genes annotated for functions in lipid synthesis and metabolism. (bioinf.org)
Unexpectedly multiple genetic and protein-protein interactions were HMN-214 also detected between and proteins with Gene Ontology (GO) annotations indicating direct involvement HMN-214 in vesicular transport secretory pathway and cellular localization: of 178 ERG25-interacting proteins 53 had such GO annotations representing a highly significant enrichment (e.g. vesicle-mediated transport p=1.4*10?8) (Fig. 3B). (bioinf.org)
On Mouse monoclonal to eNOS the other hand and which didn't affect response to EGFR-targeting agencies interacted with just 8 and 7 non-sterol pathway genes respectively and fewer genes general (Fig. S4B). (bioinf.org)
A fly-human cross-species comparison of the phosphoinositide-3-kinase (PI3K) interactome was conducted in a Drosophila S2R+ cell line and several NSCLC and human multiple myeloma cell lines to identify conserved interacting proteins to PI3K, a critical signaling regulator of the AKT pathway. (sdbonline.org)
MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. (icr.ac.uk)

The phosphatase activity of PTEN proteins was improved after FMG destined to PTEN proteins, indicating that PTEN is among the FMG targeted protein. (buyresearchchemicalss.net)
Collectively, these outcomes provide mechanistic understanding in to the anti-NSCLC of FMG by improving the phosphatase activity of PTEN, and claim that FMG could possibly be like a potential choice for lung tumor treatment. (buyresearchchemicalss.net)

Yet, medical immunoassays (for example, immunohistochemistry) are limited to 5 proteins due to spectral imaging limitations with conventional filter units22,23 and difficulty in de-staining' cells (eliminating antibody probes). (irjs.info)
Separating proteins by molecular mass (or mobility) before the immunoassay can determine off-target, non-specific antibody binding27. (irjs.info)
Spatially resolving proteins by size 1st allows a single antibody probe to detect multiple, unique protein forms28. (irjs.info)

Monocytes/macrophages support mammary tumor invasivity by co-secreting lineage-specific EGFR ligands and a STAT3 activator. (mpg.de)
Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. (icr.ac.uk)

Moreover, PRL-3 induced cellular addiction to EGFR signaling, as evidenced by the pronounced reversion of these oncogenic attributes upon EGFR-specific inhibition. (nus.edu.sg)
There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. (biomedcentral.com)
The SH2-PLA assay utilizes oligonucleotide-conjugated anti-GST and anti-EGFR antibodies recognizing a GST-SH2 probe and cellular EGFR, respectively. (biomedcentral.com)

PTP-sigma (a transmembrane protein tyrosine phosphatase) is a just lately discovered receptor for CSPGs and is essential for proper CSPG operation. (anaheimpoliceassociation.org)

degradation and trafficking of EGFR and its own dimerization companions ERBB2 and ERBB3. (bioinf.org)
Metabolic block upstream of SC4MOL with ketoconazole or CYP51A1 siRNA rescued cancer cell EGFR and viability degradation. (bioinf.org)
PTPN23 is a regulator of endosomal trafficking functioning to move activated membrane receptors, such as EGFR, from the endosomal sorting complex towards the ESCRT-III complex for multivesicular body biogenesis, lysosomal degradation, and subsequent downregulation of receptor signaling. (edu.sa)
DUSP3 regulated OCLN ubiquitination and degradation through decreasing OCLN tyrosine phosphorylation directly or through suppressing focal adhesion kinase, the OCLN kinase. (biomedcentral.com)

On the contrary, knockdown of Sprouty proteins increases proliferation of activated astrocytes and, consequently, reduces secondary brain damage in neuronal lesion models such as kainic acid-induced epilepsy or endothelin-induced ischemia. (springer.com)
60 identical and the kinase domains are a lot more than 85 identical.three Catalytically active Akt regulates the function of numerous substrates involved in cell survival, development, proliferation, metabolism and protein synthesis (reviewed in Manning, Cantley4). (bet-bromodomain.com)

Although important, the capability to multiplex and assay a wide range of protein focuses on (including intracellular signalling pathways) has been limited20. (irjs.info)

First, single-stage immunoassays have difficulty with multiplexed measurements of surface and intracellular proteins for each solitary cell21. (irjs.info)
Circulation cytometry also suffers from multiplexing shortcomings, especially with intracellular protein focuses on. (irjs.info)
Protein tyrosine kinase 6 (PTK6, also called BRK) is a SRC-related intracellular tyrosine kinase expressed in normal epithelia and cancer. (oncotarget.com)

Progesterone receptor membrane component 1 (PGRMC1) is 195 residue membrane-bound protein which contains a short luminal peptide, a single N -terminal transmembrane domain, and a C -terminal cytochrome b 5 -related heme-binding domain ( Figure 1A ). (oncotarget.com)
Phosphorylation of tyrosine residue 342 in the PTK6 activation loop corresponds with its activation. (oncotarget.com)
Similar to findings in the prostate, we detect nuclear and cytoplasmic PTK6 in normal mammary gland epithelial cells, but no phosphorylation of tyrosine residue 342. (oncotarget.com)

Through its activity on ligandless EGFR at plasma membrane, PTPRG regulates the autocatalytic activity of EGFR and influences the responsiveness of a cell to EGF dose. (tu-dortmund.de)
Protein kinase C regulates human pluripotent stem cell self-renewal. (rndsystems.com)
Erk2 but not Erk1 regulates crosstalk between Met and EGFR in squamous cell carcinoma cell lines. (mpg.de)

However, neither protein level of EGFR nor phosphorylation of EGFR (Y1068 and Y1173) presented obvious difference according to PTPRK transcripts in the corresponding tumours. (cardiff.ac.uk)

Tyrosine phosphorylations of IR and ERK had been determined by Traditional western blotting with anti-phospho-IRand anti-phospho-ERK1/2 antibodies and had been normalized with IR and ERK proteins, respectively, that have been then determined as fold adjustments of insulin only. (gasyblog.com)

Another PTP involved in the immune processes is PTPMEG, a cytosolic phosphatase expressed in the thymus that is able to dephosphorylates TCRζ ITAMs in vitro . (biomedcentral.com)

Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. (icr.ac.uk)
Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. (icr.ac.uk)

The assay detected signal across at least 3 orders of magnitude of lysate input with a linear range spanning 1-2 orders and a low femtomole limit of detection for EGFR phosphotyrosine. (biomedcentral.com)
We showed for the first time that interactions between SH2 domain probes and EGFR in cell lysate can be determined in a microliter-scale assay using SH2-PLA. (biomedcentral.com)
Proximity ligation assay, immunoblotting, and phosphatase assay were performed to study the effect of DUSP3 on the TJ protein occludin (OCLN). (biomedcentral.com)

Furthermore, by maintaining the plasma membrane density of EGFR due to its interaction with ligandless EGFR and dephosphorylation of EGFR at Y1045 - a cCbl-ubiquitin ligase binding site, PTPN2 participates in a spatially established negative-feedback that is mediated by vesicular recycling. (tu-dortmund.de)
However, in human breast tumors, striking PTK6 expression and phosphorylation of tyrosine 342 is observed at the plasma membrane. (oncotarget.com)

Mechanistically, α-catenin acetylation releases the transcriptional coactivator Yes-associated protein 1 (Yap1) by blocking the interaction between α-catenin and Yap1, and promotes the accumulation of Yap1 in the nucleus. (bvsalud.org)

The obvious benefit of this method is that the low sample requirement allows detection of SH2 binding in samples which are difficult to analyze using traditional protein interaction assays. (biomedcentral.com)
Predicted regulatory interaction sites for SH2- and SH3-domain proteins are in non-structured regions that could be available to cytoplasmic enzymes. (oncotarget.com)
Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). (icr.ac.uk)

Metformin and salinomycin as the best combination for the eradication of NSCLC monolayer cells and their alveospheres (cancer stem cells) irrespective of EGFR, KRAS, EML4/ALK and LKB1 status. (mpg.de)

Since the protein lacks enzymatic activities, it will be difficult to develop chemical compounds capable to directly and specifically modulate Sprouty functions. (springer.com)

STP, serine/threonine protein phosphatase. (rupress.org)

Protein Tyrosine kinase 6 (PTK6/BRK) is overexpressed in the majority of human breast tumors and breast tumor cell lines. (oncotarget.com)
Since oxidative stress activates protein kinase D1 (PKD1) in tumor cells, we investigated the effect of excitotoxicity on neuronal PKD1 activity. (nature.com)

Background Protein tyrosine phosphatase kappa (PTPRK) is a phosphatase which is recognised as a tumour suppressor. (cardiff.ac.uk)

Although mass spectrometry can measure most protein isoforms, the analytical level of sensitivity is insufficient for detection of important signalling proteins with single-cell resolution26. (irjs.info)
Still, the analytical level of sensitivity of slab-gel western blotting requires pooling of cells to accomplish detectable protein concentrations, which obscures important CTC-to-CTC protein manifestation level variance. (irjs.info)

In conclusion, the present study points out a slight potential role for PTPH1 in spontaneous pain sensitivity and it indicates that this phosphatase might play a role in the positive regulation of the LPS-induced cytokines release in vivo , in contrast to previous reports indicating PTPH1 as potential negative regulator of immune response. (biomedcentral.com)

We detected PTK6 mRNA and protein expression in the immortalized normal MCF-10A human mammary gland epithelial cell line, and examined PTK6 expression and activation in a normal human breast tissue microarray, as well as in human breast tumors. (oncotarget.com)

Furthermore, FMG regulated manifestation of some marker proteins highly relevant to cell apoptosis, invasion and migration. (buyresearchchemicalss.net)
Excitotoxic production of ROS elevates death-associated protein kinase (DAPK) activity, which provokes neuronal apoptosis in cerebral ischemia and seizure models 8 . (nature.com)

The presentation of the case may provide some selective therapeutic thoughts for NSCLC patients with acquired EGFR Leu792H mutation suffering resistance to the third-generation TKIs. (biomedcentral.com)

Functionally, PRL-3-induced hyperactivation of EGFR correlated with increased cell growth, promigratory characteristics, and tumorigenicity. (nus.edu.sg)
Yet, unlike single-cell genomics and transcriptomics, improvements in single-cell protein assays are lagging. (irjs.info)
Strikingly, most single-cell protein assays (for unmodified endogenous focuses on) are single-stage immunoassays, including enzyme-linked immunosorbent assays (with direct or sandwich readout) and immunocytochemistry, as well as newer immunoassay types designed to improve multiplexing using spatial barcoding15,16 or mass cytometry17. (irjs.info)
To surmount this Geldanamycin space, we recently launched a single-cell resolution western blot29 optimized for study of protein manifestation in each of thousands of single, cultured neural stem cells30 and glioblastoma cells31. (irjs.info)
CD45 is a protein tyrosine phosphatase receptor that is critically important for T and B cell antigen receptor-mediated activation. (techblessing.com)
Overexpression of Csk-binding protein contributes to renal cell carcinogenesis. (mpg.de)
UV-induced EGFR signal transactivation is dependent on proligand shedding by activated metalloproteases in skin cancer cell lines. (mpg.de)

Tyrosine phosphorylation is a central mechanism in the control of key signaling proteins involved in innate immunity. (biomedcentral.com)

Membrane proteins from cultured embryonic Drosophila neuronal cells. (uiowa.edu)

Anatomy21

Organisms9

Diseases7

Chemicals and Drugs145

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes49

Disciplines and Occupations1

Information Science3

Epidermal8

Kinases17

Inhibitors4

Residues3

Homology7

Mutations3

Inhibitor1

Specificity4

SHP28

PTP1B2

Downstream5

Signal transduction3

PTPs3

Exon 19 deletion1

Anti-EGFR antibody2

MAPK2

Pathway6

PTEN2

Antibody3

Ligands2

Cellular3

Transmembrane protein1

Degradation4

Proliferation2

Pathways1

Intracellular3

Residue3

Regulates3

Y10681

Antibodies1

Dephosphorylates1

MAPKs2

Assay3

Plasma membrane2

Transcriptional1

Interaction3

KRAS1

Modulate1

Serine1

Tumor2

Suppressor1

Level of sensitivity2

Sensitivity1

Epithelial cell1

Apoptosis2

Therapeutic1

Cell7

Mechanism1

Embryonic1