MutagenesisMutagenesis, Site-DirectedMutagenesis, InsertionalMolecular Sequence DataAmino Acid SequenceBase SequenceMutationBinding SitesEscherichia coliModels, MolecularPlasmidsKineticsRecombinant ProteinsAmino Acid SubstitutionStructure-Activity RelationshipEfficiencySequence Homology, Amino AcidProtein Structure, TertiaryCloning, MolecularBacterial ProteinsDNA Transposable ElementsProtein BindingCell LineProtein ConformationSubstrate SpecificityCatalysisEfficiency, OrganizationalDNA PrimersSequence AlignmentGenetic VectorsTransfectionCysteinePoint MutationGenes, BacterialProtein Structure, SecondaryDNAMutagensAlanineEthylnitrosoureaEscherichia coli ProteinsCatalytic DomainRecombinant Fusion ProteinsConserved SequenceTranscription, GeneticPhenotypeDNA Mutational AnalysisCrystallography, X-RayHistidineDNA, BacterialGenetic Complementation TestSaccharomyces cerevisiaeProtein EngineeringRestriction MappingSOS Response (Genetics)Sequence DeletionPolymerase Chain ReactionOligodeoxyribonucleotidesPromoter Regions, GeneticLysineCricetinaeEnzyme StabilityDirected Molecular EvolutionUltraviolet RaysMutant ProteinsEthyl MethanesulfonateAmino Acid MotifsDNA RepairTemperatureDNA-Binding ProteinsAspartic AcidHydrogen-Ion ConcentrationTransformation, GeneticNucleic Acid ConformationGene Expression Regulation, BacterialCOS CellsProtein BiosynthesisCattleCodonRecombination, GeneticSequence Analysis, DNADNA-Directed DNA PolymeraseAsparagineRNA, MessengerModels, BiologicalGene Transfer TechniquesGenetic EngineeringHeLa CellsTranscription FactorsSerineAmino AcidsCHO CellsGene DeletionArginineHydrolysisCercopithecus aethiopsLigandsCells, CulturedTransformation, BacterialOligonucleotidesGenes, Reporter