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  • mitochondria
  • Inasmuch as CPA and FCCP reversed the facilitation of secretion triggered by K + in the presence of NaHS and GYY, is seems that such facilitation is tightly coupled to Ca 2+ handling by the ER and mitochondria. (springer.com)
  • In parallel, there is an increasing realization in the pharmaceutical industry that candidate drugs must be tested at an early stage for adverse effects on mitochondria [ 14 ]. (biochemj.org)
  • 50 mmHg) and the mitochondria inhibitor FCCP reversibly increased [Ca(2+)]c, that is La(3+)-sensitive. (curehunter.com)
  • Therefore, we applied inducible PC12 cell model of HD to determine the relationship between the effect of expression of wild type and mutant huntingtin (Htt and mHtt, respectively) on cell survival and mitochondria functioning in intact cells under conditions of undergoing cell divisions. (frontiersin.org)
  • Because after 48 h of Htt and mHtt expression differences in mitochondria functioning co-occurred with differences in the cell viability, we decided to estimate the effect of Htt and mHtt expression lasted for 48 h on VDAC functioning. (frontiersin.org)
  • They address mHtt effects on mitochondrial bioenergetics and biogenesis, protein import complex assembly, fission and fusion, mitochondrial transport including Ca 2+ and metal homeostasis, and the degradation of damaged mitochondria via autophagy (mitophagy). (frontiersin.org)
  • Our results indicate that after cytochrome c release the activation of caspases feeds back on the permeabilized mitochondria to damage mitochondrial function (loss of Delta Psi m) and generate ROS through effects of caspases on complex I and II in the electron transport chain. (nih.gov)
  • Only those biguanides that enter mitochondria and inhibit complex I activate AMP kinase, strengthening links between complex I and the downstream effects of biguanide treatments. (biomedcentral.com)
  • These effects, together with a significant increase of basal ATP content and oxygen consumption rate, and a significant decrease of reactive oxygen species (ROS) production, provide strong evidence of an overall improvement of mitochondria bioenergetics in trisomic cells. (frontiersin.org)
  • Changes in the FA compositions of membrane lipids were correlated with changes in energy metabolism in mitochondria: the efficiency of oxidative phosphorylation and the rate of NAD-dependent substrate oxidation in the presence of ADP and FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone) were reduced. (deepdyve.com)
  • Given this mitochondrial Parkin-related effect and the reported Parkin/PINK1 interaction, we sought to determine whether PINK1 is involved in the recruitment of Parkin to the mitochondria and to define the role played by Parkin, PINK1, or both in mitochondrial turnover. (pnas.org)
  • inhibition
  • RAS inhibition prevents these effects in vivo, resulting in increased glucose tolerance and improved islet function ( 2 , 8 , 9 , 24 ). (diabetesjournals.org)
  • 9 Tet ameliorates amyloid-β(1-42)-induced spatial learning and memory impairment, and the beneficial effect of tetrandrine treatment may be linked to the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity and downregulation of interleukin-1β and tumor necrosis factor-α. (dovepress.com)
  • Conversely, although the antimalarial biguanide cycloguanil and its pro-drug proguanil inhibit isolated complex I, they do not inhibit cellular or mitochondrial respiration [ 4 ] and so, unlike the anti-hyperglycemic biguanides, they are not associated with lactic acidosis, a side effect of complex I inhibition. (biomedcentral.com)
  • In vitro data revealed that the ALDH2 activator Alda-1 and glycogen synthase kinase-3β inhibition protected against high glucose-induced mitochondrial and mechanical anomalies, the effect of which was cancelled by mitochondrial uncoupling. (biomedcentral.com)
  • mutation
  • Finally, this study showed that substitutions analogous to the constitutively active Ras-G12V mutation in dMiro's N-terminal and C-terminal GTPase domains cause neomorphic phenotypic effects that are likely unrelated to the normal function of each GTPase domain. (sdbonline.org)
  • secretion
  • Studies of isolated islets treated with angiotensin II or its blockers point to a possible role of activated local RAS or direct angiotensin II effects on impaired insulin secretion ( 15 , 17 , 25 ). (diabetesjournals.org)
  • detrimental
  • These findings suggest that the epidemiological link between environmental mercury exposure and an increased risk of developing autism may be mediated through mitochondrial dysfunction and support the notion that a subset of individuals with autism may be vulnerable to environmental influences with detrimental effects on development through mitochondrial dysfunction. (hindawi.com)
  • tissues
  • Finding a local RAS in various tissues and organs such as brain, kidney ( 12 ), heart ( 13 ), liver, and adipose tissue ( 14 ) has expanded its role to diverse physiological functions in addition to its effects on circulation. (diabetesjournals.org)
  • Based on these results, tissues were pre-exposed to 200 nM FCCP for 120 min for the subsequent studies on culture and cryopreservation. (springermedizin.de)
  • Such information would be relevant for a deeper understanding of the NAD metabolome, for the design of more potent tumor cell killing strategies based on metabolic approaches, and for the identification of rescue therapies designed to spare healthy tissues from the effects of NAD-depleting agents. (wwforum.com)
  • cellular
  • Interestingly, percentages of cellular proliferation and follicular density were unaltered by the FCCP exposures. (springermedizin.de)
  • We propose that the Warburg effect observed in aggressive STS is reversed by induction of Vnn1 pantetheinase and the rewiring of cellular energy metabolism by its products. (life-science-alliance.org)
  • depletion
  • Hence, to understand whether PARP-1 activa- tion concurs with NAD loss by Vacor, we tested the effects of three different PARP-1 inhibitors, and found that they did not affect dinucleotide depletion (Figure S1D). (wwforum.com)
  • electron
  • Here we show that both the rapid loss of Delta Psi m and the generation of ROS are due to the effects of activated caspases on mitochondrial electron transport complexes I and II. (nih.gov)
  • oxygen consumption
  • For instance, application of the saturated fatty acid palmitate had markedly different effects on power output and oxygen consumption in hearts of control mice and ob / ob mice, which are obese, insulin resistant, and have increased serum free fatty acid concentrations ( 3 ). (diabetesjournals.org)
  • physiological
  • I will write more about RMR and how it relates to RQ including different physiological states that influence RQ such as birth, what happens to RQ at the start of breast feeding, how thyroid hormones effect RQ, etc. once I get into my new apartment. (edwardjedmonds.com)
  • assess
  • We now assess the effect of the saturated fatty acid palmitate (1.2 mmol/l) on Ca 2+ handling, cell shortening, and mitochondrial production of reactive oxygen species (ROS) in freshly isolated ventricular cardiomyocytes from normal (wild-type) and obese, insulin-resistant ob / ob mice. (diabetesjournals.org)
  • glucose
  • The systemic effect of metformin lowers blood glucose levels and, hence, glucose availability to glucose-hungry cancer cells. (sciencemag.org)
  • Cells
  • The results show that palmitate has adverse, ROS-mediated effects in wild-type cardiomyocytes, whereas it improves the function of ob / ob cells. (diabetesjournals.org)
  • Here, we have investigated the effects of NaHS (fast donor of H 2 S) and GYY4137 (GYY, slow donor of H 2 S) on the exocytotic release of catecholamines from fast-perifused bovine adrenal chromaffin cells (BCCs) challenged with sequential intermittent pulses of a K + -depolarizing solution. (springer.com)
  • Abstract
  • Abstract: This study evaluates the effect of paravertebral spinal injection (PSI), utilizing both subjective and objective assessments in chronic low back pain (LBP) associated with facet joint arthrosis over a one-month duration. (preprints.org)
  • addition
  • Mitochondrial swelling in potassium phosphate and potassium acetate solutions was activated by valinomycin and to a lesser extent by the further addition of FCCP. (biochemj.org)
  • There is increasing evidence that metformin, in addition to its antidiabetic effect, has anticancer properties. (sciencemag.org)
  • Diabetes
  • The effect of diabetes is to keep the respiratory quotient low, since a respiratory quotient of one corresponds to the oxidation of pure carbohydrate, and extreme diabetics oxidize fat in preference to carbohydrate, and may have a quotient just a little above 0.7. (edwardjedmonds.com)
  • cell
  • A-D) Dose effect of Vacor on sensitive (A) and insensitive (B) cell lines at 24-hr incubation. (wwforum.com)
  • activity
  • FCCP is preferred as more specific in its activity compared to the common reagent, 2,4-dinitrophenol. (toxbank.net)
  • determine
  • Biopsies of cat ovarian tissue were first treated with various concentrations of FCCP (0, 10, 40, or 200 nM) for 10 or 120 min to determine the most suitable pre-exposure conditions. (springermedizin.de)