Superoxide DismutaseIncreased catalase activityEnzyme catalaseStimulated the activity of catalaseGlutathioneEnzymesTissuesCell lysatesActivityHydrogen peroxide concentrationTemperatureRatsAntioxidantWater and oxygenExperimentConcentrationsOxygenAbstractExposureEnzymaticELISASubstrate ConcentrationAminoLiverConcentration on the RateResveratrolExamineResultsExtractsSubunitsCarcinogenic effectsProteinSupplementationSpeciesKidneyAqueousRabbitInitiallyProtectiveAdverse effectsHumanMechanismDamageBiochemicalReaction
Superoxide Dismutase4
- This study was conducted to evaluate the effect of radiofrequency wave (RFW)-induced oxidative stress in the eye and the prophylactic effect of vitamin C on this organ by measuring the antioxidant enzymes activity including: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA). (nih.gov)
- In this report, the superoxide dismutase (SOD) and catalase (CAT) activities produced in bacteria (D.radiodurans AS1.633) and their change caused by 20keV N + beam exposure were examined. (sinap.ac.cn)
- The purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by malathion that is used as an insecticide in agriculture, on catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in kidney tissues by immunohistochemical technique. (ankara.edu.tr)
- Epididymal (visceral) and subcutaneous adipose tissue, gastrocnemius muscle, and plasma samples were collected to measure oxidative stress markers (advanced oxidation protein products (AOPP), oxidized low-density lipoprotein (oxLDL)), antioxidant system markers (ferric-reducing ability of plasma (FRAP), superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities), and prooxidant enzymes (NADPH oxidase and xanthine oxidase (XO) activities, myeloperoxidase content). (hindawi.com)
Increased catalase activity3
- The application of ascorbic acid reduced cell membrane damage and increased catalase activity in water-stressed trees of both cultivars. (frontiersin.org)
- Increased catalase activity was observed in brassinolide treated plants followed by salicylic acid and ascorbic acid under salinity. (arccjournals.com)
- The data indicate that compared to control muscles, repetitive loading significantly increased catalase activity and lowered MDA levels in the TA. (cdc.gov)
Enzyme catalase7
- In this experiment you are measuring the rate at which oxygen is formed from the enzyme catalase decomposing hydrogen peroxide. (docbrown.info)
- vary either the hydrogen peroxide or the enzyme catalase). (docbrown.info)
- Mashed up potato made into a fine slurry diluted with acts as a source of the enzyme catalase. (docbrown.info)
- The enzyme catalase, found in potato juice, was used for the catalyst along with a substrate known as hydrogen peroxide H2O2. (rftp.com)
- Catecholase is a reaction between oxygen and catechol… 3155 Words 13 Pages The Effect of Substrate Concentration on the Rate of Activity of the Enzyme Catalase Background Information: Enzymes are biological catalysts that increase the speed of chemical reactions without undergoing any physical change. (rftp.com)
- Catalase (Enzymes) Abstract In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. (educationindex.com)
- reactivity of the enzyme catalase on hydrogen peroxide while subject to different concentrations of an inhibitor. (educationindex.com)
Stimulated the activity of catalase1
Glutathione4
- In untreated rats exposed to C-H-R, there was a significant increase in malondialdehyde (MDA) levels in liver and muscle along with decreased activity of catalase (CAT) and glutathione-S-transferase (GST) in liver and muscle. (nih.gov)
- Similarly, significant increase in Lipid Peroxidation (LPO) level while induced significant decreases in the activities of liver Catalase (CAT) and Glutathione-S-Transferase (GST). (scialert.net)
- The tibialis anterior muscle (TA) was removed and assayed for biomarkers of oxidative stress that included: the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG), which is a measure of redox status, malondialdehyde (MDA) which is a biomarker for lipid peroxidation, 8hydroxy-2'-deoxyguanosine (8-OHdG), which is a marker of oxidative damage to DNA, catalase concentration, and cytosolic hydrogen peroxide (H2O2) levels. (cdc.gov)
- H₂O₂ alone resulted in a significantly decreased viability (MTT assay), depressed glucose-stimulated insulin secretion, increased apoptotic and necrotic cell death (AO/EB staining), inhibited glutathione peroxidase activity (GPx) and stimulated catalase activity that were associated with increased intracellular generation of reactive oxygen species (ROS) (fluorescence DCF). (unige.ch)
Enzymes5
- pH effects are more complex, in case of reactions involving ions, effects are related to the ionic concetnrations , but one should not forget that enzymes, being very complex macromolecular entities can function only within very specific pH range. (madsci.org)
- Catalase The structure and function of enzymes is a central theme in cellular and molecular biology. (rftp.com)
- Using a potato and hydrogen peroxide, we can observe how enzymes like catalase work to perform decomposition, or the breaking down, of other substances. (rftp.com)
- To prevent this cellular damage, organisms generate enzymes, such as catalase, that breakdown ROS into harmless substances. (bellarmine.edu)
- The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. (educationindex.com)
Tissues6
- However, "Copper deficiency can lead to a reduction in catalase activity in tissues, such as heart and liver. (wikipedia.org)
- Catalase plays a major role in the protection of tissues from the toxic effects of H_{2}O_{2} and partially reduced oxygen species. (tubitak.gov.tr)
- The purpose of this study was to compare the effects of two training modalities (moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT)) on the pro/antioxidant status of different tissues in obese Zucker rats. (hindawi.com)
- Following exposure to radioactive strontium compounds, the most severe non carcinogenic effects seen are the result of incorporation of radioactive strontium, an emitter of beta radiation, into the skeleton, with subsequent irradiation of surrounding tissues (ATSDR 2001e). (cdc.gov)
- NIR can not cause ionization however have been shown to produce other biological effects, for instance by heating, altering chemical reactions or inducing electrical currents in tissues and cells. (intechopen.com)
- Gas exchange was monitored every 10 days, and at harvest, bioaccumulation of Ce and Zn in tissues was determined by ICP-OES/MS. The effects of NPs exposure on nutrient concentration and distribution in ears were also evaluated by ICP-OES and μ-XRF. (acs.org)
Cell lysates1
- We have demonstrated successful penetration of catalase into the BHK mammalian cells using cell penetrating peptide (CPP) technology: both fluorescence from catalase conjugated with amino dye 555, and catalase activity in cell lysates, is significantly higher in CPP+catalase treated cells than in cells treated with catalase alone. (kennesaw.edu)
Activity14
- Effect of Concentration on Enzyme Activity Lab Report. (rftp.com)
- In this study, coelomocytes of L. variegatus and A. punctulata were exposed to UVB (302 nm) for one or two hours, and catalase activity was measured using colorimetric assays. (bellarmine.edu)
- The maximal activity of catalase was observed between pH 6.0 and 8.0. (tubitak.gov.tr)
- Enzyme activity was stable at temperatures between 10 and 30°C. The activity of purified catalase was inhibited by azide, cyanide, \beta -mercaptoethanol, dithiotreitol (DTT) and iodoacetamide. (tubitak.gov.tr)
- Digalloyl-resveratrol (di-GA) is a synthetic compound aimed to combine the biological effects of the plant polyhydroxy phenols gallic acid and resveratrol, which are both radical scavengers and cyclooxygenase inhibitors exhibiting anticancer activity. (nature.com)
- The effect of salinity on photosynthetic activity in potassium-deficient barley species. (arccjournals.com)
- Keramati V, Jamili S, Ramin M (2010): Effect of diazinon on catalase antioxidant enzyme activity in liver tissue of Rutilus rutilus. (ankara.edu.tr)
- The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. (educationindex.com)
- The experiment aimed to compare the effects of BA and mT on the regeneration from explants of Daphne mezereum , on the levels of several chemical components, the catalase activity, and to assess genetic diversity of microcuttings. (mdpi.com)
- Total soluble proteins and amino acids were the highest in plants from the MS medium with 1 mg·L −1 mT. Increased levels of hydrogen peroxide and catalase activity were observed on media with 1 mg·L −1 BA. (mdpi.com)
- Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. (ipl.org)
- catalase functional activity in lysates was determined using a H 2 O 2 activity assay. (bmj.com)
- The lipid peroxidation (TBARS) and the enzymatic activity of catalase (CAT) were measured in the cardiac muscle. (bvsalud.org)
- The supplementation of Trans-resveratrol attenuated the cardiac oxidative stress and increased the activity of catalase. (bvsalud.org)
Hydrogen peroxide concentration3
- You need a series of hydrogen peroxide solutions of known different concentrations and a fixed concentration of the potato mix to investigate the effect of changing the hydrogen peroxide concentration. (docbrown.info)
- You can also keep the hydrogen peroxide concentration constant and do the investigation with a set of different concentrations of the potato-catalase mixture. (docbrown.info)
- The decomposition of hydrogen peroxide by catalase proceeds according to first-order kinetics, the rate being proportional to the hydrogen peroxide concentration. (wikipedia.org)
Temperature3
- The 'stock' solutions of potato-catalase or hydrogen peroxide should be initially in separate boiling tubes and placed in the water bath so that everything starts at the right temperature. (docbrown.info)
- The effect of the extract was studied on lipid peroxidation and antioxidant parameters in liver and gastrocnemius muscle of rats on attaining the rectal temperature (T(rec)) of 23 degrees C during C-H-R exposure and after recovery (T(rec)37 degrees C) from C-H-R-induced hypothermia. (nih.gov)
- Effect of 28-homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiata. (arccjournals.com)
Rats10
- We also reported about the effects of transient and persistent hypothyroidism on testicular antioxidant defence system in mature rats to know the role of hypothyroidism-induced oxidative stress in testicular development and maturation [ 7 ]. (hindawi.com)
- Effects of antioxidant supplementation and repetitive loading on biomarkers of oxidative stress in aged rats. (cdc.gov)
- The purpose of this study was to characterize the-effects of two different dietary antioxidant supplementations and chronic repetitive loading exercise on biomarkers of oxidative stress in aged rats. (cdc.gov)
- This study evaluated the effects of fractionated ethanol extracts of Balanites aegyptiaca fruit-mesocarp (BAFM) in streptozotocin-induced diabetic rats. (scialert.net)
- In the present study, we investigated whether Sirtuin Type 1 (Sirt1), a class III histone deacetylase, mediates the retinal protective effect of HRS in rats with light-induced retinal damage. (arvojournals.org)
- Rats were treated with HRS for 5 days after intense light exposure, and then ERGs were performed and retinas were collected to evaluate the effect of HRS on Sirt1 expression. (arvojournals.org)
- This study investigated the effect of the trans-resveratrol supplementation on the cardiac oxidative stress in rats exposed to cigarette smoke. (bvsalud.org)
- Our findings evidenced the cardioprotective effect of trans- resveratrol in rats exposed to cigarette smoke. (bvsalud.org)
- At 7wk, HF-fed animals exhibited several immune alterations (blood leukocyte/neutrophil number, lymph node B-cell proportionality)-effects which were more pronounced in SD rats. (cdc.gov)
- Collectively, HF diet appeared to have a greater impact on global immune status and exposure-induced lung injury in SD rats, but a more pronounced effect on inflammation resolution in BN rats. (cdc.gov)
Antioxidant2
- Recent basic and clinical research has revealed that hydrogen is an important physiological regulatory factor with antioxidant, anti-inflammatory and anti-apoptotic protective effects on cells and organs. (researchgate.net)
- In recent years, molecular hydrogen treatment has the potential to preventive and therapeutic applications against many diseases due to its extensive effects, such as antioxidant, anti-inflammatory, antiapoptotic [16, 17]. (researchgate.net)
Water and oxygen4
- Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals) which catalyzes the decomposition of hydrogen peroxide to water and oxygen. (wikipedia.org)
- one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second. (wikipedia.org)
- The job of catalase in this experiment was to accelerate the breakdown of hydrogen peroxide into water and oxygen gas. (rftp.com)
- Catalase is an enzyme found in nearly all living organisms and is used as a defense against oxidative stress, specifically by degrading the hydrogen peroxide (H 2 O 2 ) into water and oxygen. (kennesaw.edu)
Experiment1
- An experiment was conducted to study the effect of plant growth regulators namely jasmonic acid (50 µM), brassinolide (0.5 ppm), salicylic acid (100 ppm), ascorbic acid (100 ppm), gibberellic acid (10 ppm), benzyl amino purine (5 ppm) and nutrient consortium (K 2 SO 4 (0.5%) + FeSO 4 (0.5%) + Borax (0.3%) and TNAU Pulse Wonder (1%) on blackgram ( Vigna mungo L.) variety TNAU (Blackgram) CO 6 under salinity condition. (arccjournals.com)
Concentrations2
- Furthermore, the poison cyanide is a noncompetitive inhibitor of catalase at high concentrations of hydrogen peroxide.Arsenate acts as an activator. (wikipedia.org)
- 2005): Effect of sublethal concentrations of permetherin on ovary activation in the predator. (ankara.edu.tr)
Oxygen3
- To this end, catalase is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less-reactive gaseous oxygen and water molecules. (wikipedia.org)
- The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen, where a higher concentration of inhibitor will yield less oxygen, resultant of a lower rate of reaction. (educationindex.com)
- The oxyhemoglobin dissociation curve (see the image below) has a characteristic sigmoid shape due to the cooperative effect that exists between the multiple oxygen binding sites on the hemoglobin molecule. (medscape.com)
Abstract1
- ABSTRACT This study aimed to determine the occurrence of Mycobacterium avium complex and other nontuberculous mycobacteria in drinking-water in Basra governorate, Iraq and their susceptibility to several antibiotics and the effect of 0.5 mg/L of chlorine on their survival. (who.int)
Exposure3
- Elevated catalase activities under UVB, a range of UVR from 280-315 nm, exposure have been detected for many aquatic organisms. (bellarmine.edu)
- Gerdy GF, Rose HA, Starey NH (1987): Effect of lenght of exposure to malathion on xenobiotic biotransformation in male rat liver. (ankara.edu.tr)
- This information may serve to aid in interpreting assessments in risk models, epidemiologic studies, and development of occupational exposure limits, relating to health effect endpoints identified in toxicological studies considering similar instruments evaluated in this study. (cdc.gov)
Enzymatic3
- Hydration effects are affected by pH as well, and one should not forget that substrates of the enzymatic reactions are oftenly reversibly or irreversibly affected by pH of the medium. (madsci.org)
- Some General Chemistry textbooks also contain brief explainations of the pH effects in enzymatic catalysis. (madsci.org)
- 2007): Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver Catfish (Rhamdia Quelen). (ankara.edu.tr)
ELISA2
- These pathways complement our catalog of research reagents for the study of Teratogenic Effect including antibodies and ELISA kits against PAEP, AHR, TNF, INS, SS18L1. (novusbio.com)
- We have 3691 products for the study of Teratogenic Effect that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits. (novusbio.com)
Substrate Concentration1
- The Effect of Substrate Concentration, Enzyme. (educationindex.com)
Amino2
- Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. (wikipedia.org)
- The amino acid sequence of bovine catalase was determined in 1969, and the three-dimensional structure in 1981. (wikipedia.org)
Liver3
- In 1937 catalase from beef liver was crystallized by James B. Sumner and Alexander Dounce and the molecular weight was measured in 1938. (wikipedia.org)
- However, catalase deficiency in mice may increase the likelihood of developing obesity, fatty liver, and type 2 diabetes. (wikipedia.org)
- Uncommon side effects that have been reported include severe rash, liver damage, and severe allergic reactions. (webmd.com)
Concentration on the Rate1
- investigate the effects of the increase in the enzyme concentration on the rate of reaction. (educationindex.com)
Resveratrol3
- The necessity of Sirt1 for the retinal protective effect of HRS was investigated using the Sirt1 activator resveratrol, the Sirt1 inhibitor EX-527, and short interfering RNAs. (arvojournals.org)
- The Sirt1 activator resveratrol mimicked the effect of HRS in light-damaged retinas. (arvojournals.org)
- A suplementação com trans-resveratrol atenuou o estresse oxidativo cardíaco e aumentou a atividade enzimática de defesa catalase. (bvsalud.org)
Examine3
- These variations of environmental factors are use to conduct experiments to examine the effects it have on catalase and lipase. (ukessays.com)
- To examine the effect of catalase gene transfer on survival of corneal endothelial cells (EC) following challenge with hydrogen peroxide (H 2 O 2 ) in an ex vivo model of oxidative stress. (bmj.com)
- To examine the morphological effects of catalase gene transfer in modulation of H 2 O 2 induced injury, transduced corneas were maintained in ex vivo culture and challenged with H 2 O 2 . (bmj.com)
Results8
- Graph your results as copper sulfate concentration M x-axis vs. This reaction is caused by catalase, an enzyme within the potato. (rftp.com)
- SDS-gel electrophoresis results indicated that chicken erythrocyte catalase consists of four apparently identical subunits, with a molecular weight of around 57.5 kDa. (tubitak.gov.tr)
- Here, we present results from colocalization and physiological protection of CPP delivered catalase assayed to date. (kennesaw.edu)
- These results suggested that these bacteria possess inducible defense mechanisms against the deleterious effects of oxidization. (sinap.ac.cn)
- The results suggest that mT has a more positive effect on shoot regeneration. (mdpi.com)
- The results of research in recent years confirmed its hypoglycemic, hypolipemic, and hepatoprotective effects, among others. (mdpi.com)
- In addition, the results of laboratory tests also indicate its immunomodulatory and anticancer effects, although there is still a lack of data on the mechanisms of how they are involved in the fight against cancer. (mdpi.com)
- Our results conclude that L-citrulline is a promising treatment option for treating hypertension, and L-citrulline can be combined with spironolactone to get a synergistic effect in treating hypertension. (ijpsonline.com)
Extracts1
- Erdağ D (2012): The effects of allium czelghauricum (liliaceae), lathyrus karsianus (fabaceae) and onosma nigricaule (boraginaceae) extracts on oxidation parameters in malathion treated mice. (ankara.edu.tr)
Subunits1
- Human catalase forms a tetramer composed of four subunits, each of which can be conceptually divided into four domains. (wikipedia.org)
Carcinogenic effects1
- In with carcinogenic effects (e.g. benzene, chromates, aromatic the years up to 1930, major scientific progress occurred in the fun- damental principles of protection, which included substitution, enclosure and ventilation (Luxon, 1984 ). (cdc.gov)
Protein2
- Three-dimensional protein structures of the peroxidated catalase intermediates are available at the Protein Data Bank. (wikipedia.org)
- Protein S-glutathionylation provided a method for assessing physiological effects of CPP delivered catalase. (kennesaw.edu)
Supplementation2
- However, CIT supplementation showed no apparent side effects[ 9 , 10 ]. (ijpsonline.com)
- Supplementation had no effect on catalase concentration. (cdc.gov)
Species3
- The pH optimum for other catalases varies between 4 and 11 depending on the species. (wikipedia.org)
- Dimethoate (DM), an organophosphate insecticide, has been used worldwide in agriculture and domestic for several years which has led to a variety of negative effects in non target species including humans. (scialert.net)
- The aim of the present work was to evaluate the effect of CB leachates on target species with two batteries of marine and freshwater ecotoxicological bioassays. (easychair.org)
Kidney2
- Confocal microscopy was used to quantify the colocalization of TaT-CaM delivered catalase to the peroxisome, the known subcellular location of catalase, in baby hamster kidney (BHK) cells. (kennesaw.edu)
- Bingöl SA, Kocamış H (2010): The gene expression by RT- PCR and immunohistochemical expression pattern of catalase in the kidney tissue of both health and diabetic mice. (ankara.edu.tr)
Aqueous1
- Both aqueous and ethyl acetate extract-fractions of BAFM exert hypoglycaemic, antihyperglycaemic and antilipidaemic effects. (scialert.net)
Rabbit1
Initially1
- Mice genetically engineered to lack catalase are initially phenotypically normal. (wikipedia.org)
Protective3
- 2003): Protective effects of erdosteine against doxorubicin- induced cardiomyopathy in mice. (ankara.edu.tr)
- This result supported our hypothesis that Sirt1 mediates the protective effect of HRS. (arvojournals.org)
- Additionally, the retinal protective effect of HRS was inhibited by both the Sirt1 inhibitor EX-527 and Sirt1 targeted short interfering RNAs. (arvojournals.org)
Adverse effects1
- The most important adverse effects of blood transfusion relate to the antigenicity of donor blood and its ability to transmit infections . (medscape.com)
Human2
- The optimum pH for human catalase is approximately 7, and has a fairly broad maximum: the rate of reaction does not change appreciably between pH 6.8 and 7.5. (wikipedia.org)
- Human children and weanling animals are the most susceptible populations for this effect. (cdc.gov)
Mechanism1
- While the complete mechanism of catalase is not currently known, the reaction is believed to occur in two stages: H2O2 + Fe(III)-E → H2O + O=Fe(IV)-E(.+) H2O2 + O=Fe(IV)-E(.+) → H2O + Fe(III)-E + O2 Here Fe()-E represents the iron center of the heme group attached to the enzyme. (wikipedia.org)
Damage2
- Secondly, does catalase delivered as cargo on a cell penetrating peptide protect mammalian cells against damage from H 2 O 2 ? (kennesaw.edu)
- Here, we wanted to verify the extent of inflammation and oxidative stress in mice chronically-exposed to WPS and to evaluate, for the first time, its effect on alveolar injury and DNA damage and their association with impairment of lung function. (who.int)
Biochemical2
- Biochemical parameters were studied to assess the radioprotective effect of leaf extract of M. piperita. (unboundmedicine.com)
- Physical exercise is a great ally, having beneficial effects on patients with CKD, and with that, this study was done to analyze the effects of aerobic exercise on the biochemical parameters of patients with CKD during hemodialysis. (bvsalud.org)