Early detection detection and isolation immunomagnetic. Medical search. Frequent questions

This method is based on the immunomagnetic enrichment of CTCs from patient's blood samples, with subsequent staining of these cells. (iiarjournals.org)
CTCs are present in the bloodstream at a very low concentration, thus their detection and characterization require highly sensitive and specific methods, which consist of a combination of enrichment (isolation) and detection (identification) strategies ( Table 1 ) and both steps are essential components of the identification process ( 19 ). (amegroups.org)

Other uses of antibodies in detection use magnetic beads coated with antibodies for the concentration and separation of the oocysts and cysts as described below for immunomagnetic separation (IMS) methods. (wikipedia.org)
Immunomagnetic separation involves purified antigens biotinylated and bound to streptoavidin-coated paramagnetic particles. (wikipedia.org)
Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)
This customer written article "Use of Immunomagnetic Separation, mRNA Isolation and RT-PCR for Detection of Activated Human Monocytes in Whole Blood" was published in DYNALogue 2/99 and is a good example of the combined use of cell-specific IMS followed by mRNA isolation. (thermofisher.com)
Combination of IMS (immunomagnetic separation) of monocytes from whole blood, mRNA isolation using Dynabeads Oligo (dT)25, reverse transcription (RT) and polymerase chain reaction (PCR) provides a powerful method for detecting such changes at the mRNA-level in activated monocytes. (thermofisher.com)
For any patient with HUS without a culture-confirmed STEC infection, stool can be sent to a public health laboratory or to CDC through their public health laboraotory for immunomagnetic separation (IMS) techniques that can increase the sensitivity of culture. (cdc.gov)
Lab M is adding to its range of Captivate™ antibody coated paramagnetic particles for the specific immunomagnetic separation (IMS) of micro-organisms. (rapidmicrobiology.com)

The large heterogeneity of melanoma CTCs has hindered their detection and clinical application. (nature.com)
The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)
To explore the heterogeneity of CTCs, a number of antibody-based and transcript-based assays have been developed for detection of a broader range of melanoma CTCs. (nature.com)
in this scenario, careful consideration must be given to the isolation approach, whereas methods that discriminate against certain subpopulations may result in the exclusion of CTCs that carry biological relevance. (oaepublish.com)
After isolation, the source and genetic make-up of CTCs are characterized using nucleic acid-based methods, such as quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), or cytometric-based methods, such as flow cytometry and enzyme-linked immunospot assay technology ( 4 ). (iiarjournals.org)
We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers. (iiarjournals.org)
Numerous studies suggest that detection of CTCs in the blood or disseminated tumour cells (DTCs) in the bone marrow of patients with cancer is linked to poor outcomes of the disease and reduced treatment efficiency ( 5 , 6 ). (iiarjournals.org)
There are several different methods for the detection of DTCs and CTCs from bone marrow or blood ( 7 - 9 ). (iiarjournals.org)
In this study, we present a novel nucleic acid-based method for the detection and possible quantification of CTCs utilizing quantitative TaqMan real time PCR. (iiarjournals.org)
The enumeration of circulating tumor cells (CTCs) represents an effective prognostic and predictive biomarker, which is able to monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease earlier than traditional imaging methods. (amegroups.org)
In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)
The aim of this review is to describe the current state of the art on CTCs detection and clinical use, the evidence to demonstrate their clinical validity, and their potential impact for both future clinical trial design and, decision-making process in our daily practice. (amegroups.org)
Herein, we review the history and the current status of CTC detection, discuss the prognostic, predictive, and diagnostic capabilities of these technologies, and describe application of CTCs as models for the study of tumor biology. (amegroups.org)
The detection of CTCs in peripheral blood is one of the most promising approaches to identify disseminated disease in colorectal cancer (CRC). (researchsquare.com)
CTCs were detected in all stages of the Union of International Cancer Control (UICC), in stage I (1/4, 25%), in stage II (4/12, 33.3%), in stage III (5/19, 26.3%) in stage IV (21/33, 63.6%).The detection of CTCs was associated to the UICC stage (p = 0.035) and to the presence of distant overt metastases (p = 0.014). (researchsquare.com)
In conclusion, detection of CTCs is an independent and strong prognostic factor in CRC, which might improve the identification of high-risk patients in future clinical trials. (researchsquare.com)
The isolation and molecular analysis of CTCs in peripheral blood is one of the most promising approaches to identify disseminated disease, in order to upgrade or downgrade the multimodal therapy. (researchsquare.com)

Magnetic beads are versatile tools for exosome isolation and downstream analysis. (exosome-rna.com)

In this review, the current and novel methods for detection of breast cancer metastasis, as well as the mechanisms involved in metastasis and the treatment of breast cancer metastasis, are discussed. (iiarjournals.org)
Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
Computed-tomography and magnetic resonance imaging should be performed if suspicion arises from the earlier mentioned methods ( 6 ). (iiarjournals.org)
Although mammographic screening has reduced the mortality rate associated with metastasis as a result of early diagnosis ( 2 ), the methods described above are frequently inept at detecting metastasis at the earliest stage and at accurately predicting the clinical outcome of the disease ( 3 , 4 ). (iiarjournals.org)
Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)
Developing early screening, diagnosis and treatment methods can effectively reduce the mortality of CRC. (creative-proteomics.com)
The dissemination of tumor cells from the primary tumor to distant sites through bloodstream cannot be early detected by standard imaging methods. (amegroups.org)
Hence, this review reports possible methods and strategies for the fast and reliable detection of ZIKV with particular emphasis on current updates, knowledge, and new hypotheses that might be helpful for medical professionals in poor and developing countries that urgently need to address this problem. (frontiersin.org)
This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)
Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)
The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)
In this review article, we aimed to present contemporary methods and techniques for the sampling, isolation, and analysis of single cells and to give an overview of the current scientific literature about CRC at the single-cell level. (por-journal.com)
Methods: Identification of tumour-associated endothelial markers (TEM) by comparing antigen expression on normal vs t-CEC and assess the presence of t-CEC in peripheral blood of cancer patients by incorporating TEM in our novel flow cytometry-based CEC detection assay. (researchgate.net)

Diagnosis of salmonellosis depends on clinical signs and isolation of the bacteria from feces, blood, or tissues of affected animals. (clicksold.com)
This review describes the current state of the art on CTC detection strategies, the evidence to demonstrate their clinical validity, and their potential impact for both future clinical trial design and, decision-making process in our daily practice. (amegroups.org)
The differential clinical value carried within each distinct EV subset highlights the advantages of selective isolation. (biomedcentral.com)
Moreover, neither isolation approach is readily compatible with routine clinical workflows, which is still an underappreciated aspect in studies attempting to harness the potential of EVs for medical diagnostics. (biomedcentral.com)
Non-viral site directed CAR integration can be accomplished using CRISPR/Cas9 and double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) via homology-directed repair (HDR), however yields with this approach have been limiting for clinical application (dsDNA) or access to large yields sufficient to meet the manufacturing demands outside early phase clinical trials is limited (ssDNA). (biomedcentral.com)

Since blood plasma circulates through the whole body, it is often the biofluid of choice for biomarker detection in EVs. (biomedcentral.com)

These cells may be activated by a wide range of stimuli, such as immunological, inflammatory or bacterial components, to express a diversity of "early immediate genes", including Tissue Factor (TF) and Tumour Necrosis Factor a (TNFa) (1-3). (thermofisher.com)

Here, scientists from the Oslo University Hospital describe the workflow of immuno magnetic isolation and analysis of exosomes by flow cytometry, Western immunoblotting, and electron microscopy. (exosome-rna.com)
In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers. (por-journal.com)

The EasySep™ Release Human PE Positive Selection Kit is designed to isolate cells that are labeled with PE-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. (stemcell.com)

Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)

Since most studies to elucidate molecular mechanisms of EPC impairment in diabetes have been conducted in EPCs isolated from the PB in humans with a long history of diabetes, little is known about the changes occurring in EPCs located within the BM in the early stages of diabetes. (biomedcentral.com)
Efforts to identify these cells were generally unsuccessful, and their very existence was brought into question in the early 1980s by molecular biologists who failed to locate an elusive suppressor gene in the mouse MHC class II locus ( 2 ). (aai.org)

IMS increases the sensitivity of the isolation methodology and in most circumstances results are achieved 24 hours earlier than using standard protocols. (rapidmicrobiology.com)

The benefits of adhering to the recommended testing strategy include early diagnosis, improved patient outcome, and detection of infections caused by all STEC serotypes. (cdc.gov)

2005. Development of a species-specific and sensitive detection assay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil . (hutton.ac.uk)

Although these biosensors are currently among the less popular tools for human disease detection, they have become useful tools for the screening and detection of pathogenic bacteria, fungi, and viruses because of their versatile advantageous features compared to other sensing devices. (frontiersin.org)
These findings demonstrate that the involvement of LTα in EAE development is more complex than previously estimated, and that LTβR exhibits diverse functions depending on the disease stage: pathogenic at the early stage and protective at the later stages of EAE. (rusimmun.ru)

Based on review of current literature, this study examined the expression level of 35 genes that are known to be involved in endothelial progenitor cell migration and function in magnetically sorted Lin - /VEGF-R2 + endothelial progenitor cells obtained from the bone marrow of Akita mice in the early stages of diabetes (18 weeks) using RT-PCR and Western blotting. (biomedcentral.com)
Low SDF-1 expression may contribute to the dysfunctional mobilization of bone marrow Lin - /VEGF-R2 + endothelial progenitor cells, which may contribute to microvascular injury in early diabetes. (biomedcentral.com)

ELISA antibody technology has been developed to allow for readable detection by the naked eye for rapid identification of coliform microcolonies. (wikipedia.org)

Plasma-derived EVs were captured and detected using this straightforward protocol, sequentially combining isolation and staining of specific surface markers, such as CD9 or CD41. (biomedcentral.com)

In addition, ASCO also recommends mammography for the early detection of relapse in breast cancer ( 5 ). (iiarjournals.org)

Therefore, there is an urgent need to find novel biomarkers, which could monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease. (amegroups.org)

As early as 1869, cells with the morphology of cancer cells had been found by Thomas Ashworth in the blood of a person who died from metastatic cancer ( 1 ). (iiarjournals.org)
Although the improvements in BC detection and adjuvant treatments led to a significant decrease in BC-related deaths in the last two decades, about 30% of women initially diagnosed with early-stage cancer eventually develop metastatic disease. (amegroups.org)

Furthermore, EVs isolated with anti-CD61 beads enclosed mRNA expression patterns that might be associated to early-stage lung cancer, in contrast with EVs captured through CD9, CD63 or CD81. (biomedcentral.com)
2007. Maternal nutrient restriction in early pregnancy programmes hepatic mRNA expression of growth related genes and liver size in adult male sheep . (hutton.ac.uk)

Precultivation in select medium must preface detection to avoid detection of dead cells. (wikipedia.org)
The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
After a constitutive activation of the Akt pathway was initiated, early follicles (50-150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) ( n = 32), gel ( n = 24) or within matrigel ( n = 27) in serum free media for 14 days. (biomedcentral.com)
An early approach was to identify the presence of free nucleic acids released from cancer cells into the blood by cancer cell apoptosis and necrosis, as well as by active secretion ( 8 , 9 ). (amegroups.org)

CTC detection using our multimarker approach was associated with shorter overall and progression-free survival. (nature.com)
The development of this approach is currently hampered by the lack of knowledge regarding signals responsible for early follicle activation. (biomedcentral.com)

Detection of breast cancer metastasis at the earliest stage is important for the management and prediction of breast cancer progression. (iiarjournals.org)

In conclusion, EMP-PDI promotes early platelet activation through glycoprotein (GP) IIb/IIIa receptors present on platelet surface in the diabetic state. (aging-us.com)
We suggest that co-culture with activated ovarian ECs promotes early follicular development and survival. (biomedcentral.com)

Aiming to provide proof-of-concept evidence and methodology for the potential role of serum-derived EVs as biomarkers in leukemic versus healthy samples in this study, we hope to pave the way for future detection of promising biomarkers for imminent disease progression and the identification of potential targets to be used in a therapeutic strategy. (nature.com)

This enables for easy detection and avoids the need for isolation of pure cultures and confirmatory tests. (wikipedia.org)
We demonstrate that co-culture with ovarian ECs enables culture of primary and early secondary mouse ovarian follicles. (biomedcentral.com)

Therefore efforts should be made to improve detection efficiencies from these samples. (iiarjournals.org)
To normalise the measurements in different samples due to variations in the isolation steps (IMS and mRNA), the expression of the housekeeping-gene, b-actin, was quantified (4). (thermofisher.com)
Captivate™ accelerates the isolation of micro-organisms, primarily from food and environmental samples. (rapidmicrobiology.com)
A total of forty commercially available samples, randomly collected from different locations were investigated and the isolation frequencies of myco-contaminants were statistically analyzed. (alksignaling.com)

The beads were then separated from the lysate using the Dynal MPC magnet, and the lysate either frozen at -70°C or immediately subjected to mRNA isolation. (thermofisher.com)

This situation has created an alarming public health emergency of international concern requiring the detection of this life-threatening viral candidate due to increased cases of newborn microcephaly associated with ZIKV infection. (frontiersin.org)

The aim of this study was to identify early diabetes-related changes in the expression of genes that have been reported to be closely involved in endothelial progenitor cell migration and function. (biomedcentral.com)

mRNA Isolation from Monocytes in While Blood. (thermofisher.com)
Blood monocytes are "blood stream passengers" en route to various tissue compartments where they are involved in numerous functions, including defence mechanisms, producing coagulation factors and cytokines, and phagocytosing various bacterial constituents and cellular debris (apoptotic bodies). (thermofisher.com)

Risk-adapted therapies for AML, ALL, and CLL patients consist of chemotherapy with/without stem cell transplantation (SCT), but the rate of early failures and relapses is still unsatisfying. (nature.com)

Total coliforms were used as fecal indicators by public agencies in the US as early as the 1920s. (wikipedia.org)

Multiplexed digital transcript detection data was generated using the Nanostring nCounter platform and evaluated through a dedicated bioinformatics pipeline. (biomedcentral.com)

As well as the ready-made Captivate™ range, Lab M provides a custom coating service and Captivate™ can also serve as a capture system for rapid detection systems. (rapidmicrobiology.com)

The lack of radiation exposure, relatively low cost, and ability to detect early-stage atherosclerosis suggest that carotid ultrasound for CIMT and plaque detection should continue to be explored as a primary tool for CV risk stratification in young to middle-aged Navitoclax cost adults with low FRS. (alksignaling.com)

In vitro development of early follicles necessitates a complex interplay of growth factors and signals required for development. (biomedcentral.com)
Angiogenesis has also been shown as critical in the process of early follicular development. (biomedcentral.com)

Anatomy10

Organisms8

Diseases11

Chemicals and Drugs28

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Psychiatry and Psychology4

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Disciplines and Occupations6

Anthropology, Education, Sociology and Social Phenomena1

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