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  • e2f5
  • In contrast, the repressing E2Fs, namely E2F3b, E2F4 and E2F5, have the ability to bind similar promoter regions to those bound by the activating E2Fs ( 11 ), but are simultaneously bound by pocket proteins (pRb, p107, or p130) that physically prevent interaction with the transcriptional machinery ( 12 ). (jimmunol.org)
  • target genes
  • In patients treated with the CDK4/6 inhibitor palbociclib before surgery, treatment significantly decreased expression of 24 of the 47 most upregulated genes in letrozole-resistant tumors, including 18 of the 20 E2F4 target genes. (aacrjournals.org)
  • In long-term estrogen-deprived ER + breast cancer cells, palbociclib also downregulated all 20 E2F4 target genes and P-RB levels, whereas the ER downregulator fulvestrant or paclitaxel only partially suppressed expression of this set of genes and had no effect on P-RB. (aacrjournals.org)
  • All cases were assigned a relapse risk according to the metagene based on average expression of 199 E2F4 target genes in each standardized dataset. (biomedcentral.com)
  • Sequence
  • A synthetic peptide corresponding to a sequence in the middle region of human E2F4(228-243aa LPKPALAQSQEASRPN), different from the related mouse and rat sequences by four amino acids. (bosterbio.com)
  • tumors
  • We analyzed the predictive value of the E2F4 metagene for the pathological response to chemotherapy in the 582 HR + /HER2 − tumors (12% pCR rate). (biomedcentral.com)
  • HR + /HER2 − tumors with an E2F4 high-risk signature were more sensitive to anthracycline-based chemotherapy than low-risk tumors, as already reported with other prognostic signatures [ 2 , 3 ] - including in the adjuvant setting [ 4 , 5 ], where high-risk tumors showed greater benefit from chemotherapy than low-risk tumors. (biomedcentral.com)
  • mice
  • Both the erythroid and the lymphoid defects in E2F4-deficient mice could be explained if the defect occurred at the level of the HSC, which gives rise to both lineages. (jimmunol.org)
  • cells
  • We analyzed the impact of E2F4 deficiency on early steps in mouse hematopoietic development, and found defects in early hematopoietic progenitor cells that were propagated through common lymphoid precursors to the B and T lineages. (jimmunol.org)
  • Among these, E2F4 is found at high concentrations in hematopoietic cells ( 15 ). (jimmunol.org)
  • function
  • Interestingly, mRNA expression of E2F4 itself did not predict for the response to chemotherapy, demonstrating the interest of the metagene as a better indicator of E2F4 function than E2F4 expression level alone. (biomedcentral.com)
  • cell
  • These findings emphasize the significance of E2F4 in controlling gene expression and cell fate. (jimmunol.org)
  • The clinical relevance comes from its independent prognostic value and its biological significance (mainly regulation of cell cycle and proliferation, reflecting high E2F4 activity). (biomedcentral.com)
  • However
  • However, E2F4 migrates as an heterogeneous set of bands between 57-64 kDa which are associated with extensive phosphorylations (Beijersbergen et al. (atlasgeneticsoncology.org)
  • However, the impact of E2F4 deficiency on thymocyte development has been controversial ( 15 , 17 ). (jimmunol.org)