• It can mediate both cell proliferation and p53-dependent/independent apoptosis. (wikipedia.org)
  • As a consequence, any modulations of the target transcript expression, miRNAs can affect various signalling pathways and cellular process such as apoptosis, proliferation or differentiation. (ukessays.com)
  • Cancer genetics, DNA damage response, apoptosis, cell cycle My current research is focused on two transcription factors that respond to DNA damage and play important roles in regulating tumor development. (mdanderson.org)
  • The first project focuses on E2F1, a regulator of genes important for cell cycle progression and apoptosis. (mdanderson.org)
  • E2F1 (UniprotKB: Q01094), belongs to the E2F family of transcription factor (TF), has a central role in regulating cell cycle progression and apoptosis [ 11 , 12 ] and, therefore, E2F1 activity is tightly governed by multiple feedback mechanisms and by the tumour suppressor Rb. (biomedcentral.com)
  • The E2F1/DP complex appears to mediate both cell proliferation and apoptosis. (innatedb.com)
  • Compound 51 inhibited the proliferation of 13 out of 15 cancer cell lines with IC50 values between 0.27 and 6.9 muM, which correlated with the complete suppression of retinoblastoma phosphorylation and the onset of apoptosis. (proteopedia.org)
  • Methylation by PRMT1 hinders methylation by PRMT5, which augments E2F-1-dependent apoptosis, whereas PRMT5-dependent methylation favors proliferation by antagonizing methylation by PRMT1. (embl.de)
  • The ability of E2F-1 to prompt apoptosis in DNA damaged cells coincides with enhanced PRMT1 methylation. (embl.de)
  • He then did post-doctoral studies with Joseph Nevins at Duke University studying the control of Rb/E2F induction of proliferation and apoptosis. (umn.edu)
  • miRNAs can post-transcriptionally regulate the expression of myriad of different target genes including more than 30% of protein coding genes 9 , thereby managing an extensive spread of biological functions such as cellular proliferation 10 , apoptosis 11 and differentiation 12 . (ajmb.org)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • Both inhibition of WDR5 by siRNA and OICR-9429 could reduce proliferation, and increase apoptosis and chemosensitivity to cisplatin in vitro and in vivo . (thno.org)
  • These data revealed that targeting WDR5 suppressed proliferation, enhanced apoptosis, chemosensitivity to cisplatin and immunotherapy in PCa. (thno.org)
  • Furthermore, these genes were classified into 11 functional groups including transcription factors, cell cycle related factors, apoptosis regulators, kinases and metabolism-related proteins, etc. (edu.hk)
  • As a result, various physiological functions, such as differentiation, cell cycle control, apoptosis regulation may stop working, and cell motility and invasion may increase. (unife.it)
  • Arf inhibits Mdm2 allowing p53 to lead the cell to growth arrest or apoptosis. (wikipathways.org)
  • Research into apoptosis, necrosis, autophagy, and cellular senescence is very important for understanding the intracellular functions that control cell survival and death. (dojindo.com)
  • STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
  • Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)
  • Embryogenesis involves orchestrated processes of cell proliferation and differentiation. (biologists.com)
  • Embryogenesis depends on the timely proliferation of progenitor cells and their subsequent differentiation into multiple cell types. (biologists.com)
  • Regulation of the timing of cell differentiation and cell fate choice are key issues for making organs of the right size, shape and cell composition. (biologists.com)
  • In many organs, cell proliferation and differentiation are antagonistically regulated by multiple basic helix-loop-helix (bHLH) activator and repressor genes. (biologists.com)
  • For example, in the developing nervous system of mouse embryos,progenitor cells proliferate and sequentially give rise to different types of cells by changing their differentiation competency. (biologists.com)
  • In this primer, we describe the key features of Hes factors and detail their roles in some representative processes of embryogenesis: namely, in the development of the nervous and digestive systems, two well-characterized processes, where Hes1 (and Hes3 and Hes5 in the nervous system) regulates cell proliferation and differentiation, and in the process of somite segmentation, where Hes7 functions as a biological clock. (biologists.com)
  • This study aimed to explore adipogenic transcriptional dynamics before (proliferation) and after (differentiation) initial preadipocyte differentiation in ducks. (biomedcentral.com)
  • We explored 36 mRNA-seq libraries in order to study transcriptome dynamics during proliferation and differentiation processes at 6 time points. (biomedcentral.com)
  • At the proliferation stage, proliferation related pathways and basic cellular and metabolic processes were inhibited, while regulatory factors that initiate differentiation enter the ready-to-activate state, which provides a precondition for initiating adipose differentiation. (biomedcentral.com)
  • Finally, we manually assembled a proposed regulation network model of subcutaneous preadipocyte differentiation base on the expression data, and suggested that E2F1 may serve as an important link between the processes of duck subcutaneous preadipocyte proliferation and differentiation. (biomedcentral.com)
  • For the first time we comprehensively analyzed the transcriptome dynamics of duck subcutaneous preadipocyte proliferation and differentiation. (biomedcentral.com)
  • Pocket proteins Rb, p107 and p130 play central roles in cell cycle progression and differentiation. (eu.org)
  • Pocket proteins play essential roles in cell cycle progression, quiescence and differentiation, and their functional disruption is associated with human cancer. (eu.org)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • Cell viability and differentiation capacity toward the mesenchymal lineages were assessed, along with immunophenotype. (biomedcentral.com)
  • Neuronal differentiation and cell-cycle programs mediate response to BET-bromodomain inhibition in MYC-driven medulloblastoma. (cancerindex.org)
  • Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
  • RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
  • These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
  • Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. (biolegend.com)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
  • These genes encode proteins involved in DNA replication and cell cycle/proliferation that contribute to major pathways responsible for suppression of colorectal carcinogenesis pathways. (iiarjournals.org)
  • Conclusion: SCFAs inhibited the expression of genes encoding proteins involved in DNA replication and cell cycle/proliferation of human colorectal cancer cells and exerted antiproliferative activity via different pathways. (iiarjournals.org)
  • The mammalian Hes basic helix-loop-helix repressor genes play central roles in these processes by maintaining progenitor cells in an undifferentiated state and by regulating binary cell fate decisions. (biologists.com)
  • In particular,the Hes bHLH repressor genes play an essential role in the development of many organs by maintaining progenitor cells and by regulating binary cell fate decisions. (biologists.com)
  • Without Hes genes such as Hes1 , however, progenitor cells prematurely differentiate into certain types of neurons only, and are depleted before they have proliferated sufficiently and generated all neuronal and glial cell types. (biologists.com)
  • In these processes, Hes genes function as effectors of Notch signaling, which coordinates cellular events via cell-cell interactions. (biologists.com)
  • Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. (innatedb.com)
  • In cells expressing high levels of tumor suppressor p21WAF1/Cip1, it represses E2F1-mediated transcription, leading to down-regulation of multiple E2F1 target genes encoding for S-phase specific proteins, epigenetic modulators, and miRNAs (e.g. miR-15/16), and thereby stalling progression through the S-phase of cell cycle. (oncotarget.com)
  • E2f1 mice manifest impaired GC responses, which is rescued by restoring EZH2 expression, thus defining a positive feedback loop in which EZH2 controls GC B cell proliferation by suppressing CDKN1A, enabling cell cycle progression with a concomitant phosphorylation of Rb and release of E2F1.The histone methyltransferase EZH2 silences genes by generating H3K27me3 marks. (cornell.edu)
  • Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. (elifesciences.org)
  • The function of differentially expressed E2Fs and their neighboring genes were mainly linked to P53 signaling pathway, cell cycle, and oocyte meiosis. (fortunepublish.com)
  • E2Fs, a set of genes that encode a family of transcription factors (TFs) in most higher eukaryotes, are generally divided into the following three subfamilies: transcriptional activator E2Fs (E2F1-E2F3), repressor E2Fs (E2F4-E2F5), and inhibitor E2Fs (E2F6-E2F8) [14,15]. (fortunepublish.com)
  • By contrast, the combination of UHRF1 depletion and histone deacetylase (HDAC) inhibition reactivated the silenced genes and strongly suppressed CRC cell proliferation. (biomedcentral.com)
  • The combination of UHRF1 depletion and HDAC inhibition also induced marked changes in the gene expression profiles such that cell cycle-related genes were strikingly downregulated. (biomedcentral.com)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • We have been interested in how the human HMGB1 gene is regulated as well as how HMGB1 regulates other genes in the cell. (edu.hk)
  • The expression profiles of these two cell lines were compared using cDNA microarray and the genes differentially expressed at a significant level were identified in these two cell lines. (edu.hk)
  • Among these genes, 96 were down regulated and 76 were up regulated in the HMGB1 suppressed MCF-7 cells. (edu.hk)
  • The expression levels of the p53 and MDM2 genes were found to be significantly lower in the HMGB1 down-regulated MCF-7 cells. (edu.hk)
  • The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
  • A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)
  • Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). (proteopedia.org)
  • Levels of retinoblastoma protein (Rb), Ki67, and Bcl-2 were measured, reflecting cellular proliferation and death. (atsjournals.org)
  • 0.01) in the epithelium of subjects with severe asthma as compared with normal subjects, indicating increased cellular proliferation. (atsjournals.org)
  • In subjects with severe asthma, as compared with subjects with mild asthma, normal subjects, and diseased control subjects, we found novel evidence of increased cellular proliferation in the airway contributing to a thickened epithelium and LR. (atsjournals.org)
  • In severe asthma, there is increased cellular proliferation in the airway contributing to a thickened epithelium and lamina reticularis. (atsjournals.org)
  • Trp and its metabolites play an essential role in regulating cellular proliferation and maintenance processes. (biomedcentral.com)
  • 1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
  • 50% compared to untreated cells and the signalling pathways responsible for DLD-1 cell growth inhibition were identified and analyzed. (iiarjournals.org)
  • The inhibition of cell proliferation was quantified using the WST-8 assay kit (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) by spectrophotometrically measuring the absorbance (450 nm) of the water-soluble formazan formed via reduction by intracellular dehydrogenases. (iiarjournals.org)
  • Altered metabolism of cancer cell can be associated with mitochondrial dysfunction which involves inhibition of OXPHOS, increase in reactive oxygen species (ROS) and promoting uncontrolled growth, that in turn further supports the development of a metastatic phenotype [ 13 , 14 ]. (dcaguide.org)
  • Most of the reports suggest that complex I activity is suppressed in cancer cells and its inhibition promotes proliferation and metastasis [ 16 - 18 ] . (dcaguide.org)
  • Targeted inhibition of miR-10b has pleiotropic effects on GBM derived cell lines, it reduces GBM growth in animal models, but does not affect normal neurons and astrocytes. (oncotarget.com)
  • We found that inhibition of miR-10b has distinct effects on specific glioma cell lines. (oncotarget.com)
  • Subsequently, miR-15/16 activities are reduced and many of their direct targets are de-repressed, including ubiquitin ligase FBXW7 that destabilizes Cyclin E. Conversely, GBM cells expressing low p21 level, or after p21 knock-down, exhibit weaker or no E2F1 response to miR-10b inhibition. (oncotarget.com)
  • This study provides fundamental insight into the functional impact of CDK4 inhibition on cells in the tumor microenvironment, which is of high importance and interest to the field. (elifesciences.org)
  • Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
  • We have discovered that E2F1 localizes to sites of both DNA double-strand breaks and UV-induced DNA damage and that this involves the phosphorylation of E2F1 by the ATM or ATR kinases. (mdanderson.org)
  • The physiological relevance of E2F1 in the DNA damage response is now being explored using a novel knock-in mouse model we developed that blocks E2F1 phosphorylation by ATM/ATR. (mdanderson.org)
  • For example, butyric acid has been found to up-regulate the expression of toll-like receptor 4 (TLR4) and the phosphorylation of MAPK and NK-κB in colon cancer cells in vitro ( 9 ). (iiarjournals.org)
  • regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. (proteopedia.org)
  • Phosphorylation of RB1 disturbs its interaction with E2F1. (proteopedia.org)
  • Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. (proteopedia.org)
  • Cancer cells have metabolic flexibility in choosing either oxidative phosphorylation (OXPHOS) or glycolysis for ATP generation depending upon the nutrient availability in tumor microenvironment. (dcaguide.org)
  • It has been reported that melanoma cells, owing to the BRAF mutation, depend majorly on glycolysis for ATP generation, and exhibit dysfunctional oxidative phosphorylation [6, 7 ] . (dcaguide.org)
  • In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
  • LIG_RB_pABgroove_1 (LxDLFD), LIG_RB_LxCxE_1 (LxCxE) and LIG_RBL1_LxSxE_2 (LxSxE).The LxDLFD motif binds to a deep groove formed between the A and B subdomains and is present in E2F family transcription factors (E2F1-5). (eu.org)
  • E2F Transcription Factors in Cancer, More than the Cell Cycle. (journalsmededu.pl)
  • 1) Identification of the molecular network responsible for the oncogenic properties of miR-221 in hepatocellular carcinomas by (a) identifying and validating upstream regulators, in particular the role of MYC and E2F1 transcription factors, and (b) identifying and validating downstream gene targets, in particular elements of cell cycle control, such as the CDKIs p27 and p57 and other potential targets identified through genome-wide transcriptome analyses. (unife.it)
  • The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
  • Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
  • Taken together, these findings indicate that miR-10b regulates E2F1-mediated transcription in GBM, in a p21-dependent fashion. (oncotarget.com)
  • IDO1 is the most studied and regulates immune cell function through the KYN pathway, and treatment combining immune checkpoint inhibitors (ICIs) with IDO1 blockade tends to inhibit tumour growth. (biomedcentral.com)
  • RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
  • We identified a novel lncRNA, SNHG29, whose expression is specifically regulated by the FLT3-STAT5 signaling pathway and is abnormally down-regulated in FLT3-ITD AML cell lines. (bvsalud.org)
  • One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
  • Furthermore, we found that heat exposure alone can significantly induce E2F1 expression. (biomedcentral.com)
  • Cancer cells are characterized by dual DNA methylation-related changes: global hypomethylation, which can induce chromosomal instability and oncogene activation, and regional hypermethylation, which is associated with transcriptional silencing. (biomedcentral.com)
  • and (2) through the paracrine release of trophic factors to induce tissue repair by endogenous cells [ 6 ]. (biomedcentral.com)
  • Doxorubicin (DOX) is known as an anticancer drug that acts in the G2/M phase of the cell cycle to arrest cell proliferation and induce cellular senescence (see the upper left figure). (dojindo.com)
  • Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells. (pharmaceuticalintelligence.com)
  • Moreover, the transcription factor E2F1 induces EZH2 during the GC reaction. (cornell.edu)
  • Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. (wikipedia.org)
  • This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. (wikipedia.org)
  • 1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
  • 2) Therefore, the proper control of protein levels is crucial for the cell cycle. (ukessays.com)
  • Cyclin-dependent kinases (CDKs) are serine/threonine protein kinases that act as key regulatory elements in cell cycle progression. (proteopedia.org)
  • The adenovirus E1A protein mimics the LxDLFD motif and competes with E2F for binding to Rb, promoting E2F activation and cell proliferation. (eu.org)
  • The most studied function of the Rb protein is in the regulation of cell cycle progression at the G1/S boundary ( Giacinti,2006 ). (eu.org)
  • The Ecdysoneless (Ecd) protein is required for cell-autonomous roles in development and oogenesis in Drosophila, but the function of its evolutionarily conserved mammalian orthologs is not clear. (unmc.edu)
  • This complete characterization of hASCs cultivated in pooled allogeneic human serum, a suitable xeno-free approach, shows that pooled allogeneic human serum provides a high proliferation rate, which can be attributed for the first time to C-MYC protein expression, and showed cell stability for safe clinical applications in compliance with good manufacturing practice. (biomedcentral.com)
  • This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)
  • Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
  • Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
  • We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • The functional regulation of cells is a complex and dynamic process. (nature.com)
  • As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
  • This review expounds the up- or down-regulation of lncRNAs in OSCC and the molecular mechanisms by which lncRNAs perform their function in the malignant cell. (biomedcentral.com)
  • Role of mammalian Ecdysoneless in cell cycle regulation. (unmc.edu)
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
  • The luciferase assay results demonstrated that HMGB1 does not regulate the p53 promoter directly but may affect the expression of p53 via its regulation on the MDM2 and the E2F1 promoters. (edu.hk)
  • The ease of motif acquisition simplifies access to basal housekeeping functions, facilitates the co-regulation of multiple biomolecules allowing them to respond in a coordinated manner to changes in the cell state, and supports the integration of multiple signals for combinatorial decision-making. (biomedcentral.com)
  • These findings indicate that E2F1 stimulates DNA repair through a non-transcriptional mechanism that functions in the context of chromatin. (mdanderson.org)
  • In addition, survival analysis revealed that SKCM patients with low transcriptional levels of E2F1/2/3/6 were associated with a significantly better prognosis. (fortunepublish.com)
  • Moreover, immune infiltrations analysis showed that transcriptional levels and somatic copy number alterations (SCNA) in E2F family were significantly correlated with several immune cell recruitments, including B cells, CD8+ T cells, CD4+ T cells, neutrophils, macrophages, and dendritic cells. (fortunepublish.com)
  • An HMGB1 expression plasmid together with the p53, the MDM2 or the E2F1 promoter-containing reporter plasmid were co-transfected into the MCF-7, the HMGB1 suppressed MCF-7 and the p53-null human osteosarcoma Saos-2 cell lines to examine the transcriptional effects of exogenous HMGB1 on these promoters. (edu.hk)
  • Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
  • We investigated the mechanism of the anti-proliferative effects of SCFAs on human colorectal cancer cells by examining their effects on gene expression. (iiarjournals.org)
  • Recently, we learned that these tumors express a gene expression "signature" that causes recruitment and accumulation of immune cells to these tumors. (umn.edu)
  • We utilize genome-wide approaches to understand the altered gene expression and 5hmC distribution in retinal cells lacking the Uhrf2 gene. (umn.edu)
  • Retinoblastoma tumor cell proliferation is negatively associated with an immune gene expression signature and increased immune cells. (umn.edu)
  • Irreversible cell cycle arrest is one of the phenomena that characterize cellular senescence. (dojindo.com)
  • Cellular senescence is controlled by various factors such as cell type and physiological conditions, such as oxidative stress. (dojindo.com)
  • E2F1 CNV was measured in genomic DNA isolated from blood of 552 patients diagnosed with melanoma and 520 healthy subjects using TaqMan Copy Number Assays. (biomedcentral.com)
  • Notably, many lncRNAs dysregulation are associated with Oral squamous cell carcinoma (OSCC) and affect various aspects of cellular homeostasis, including proliferation, survival, migration or genomic stability. (biomedcentral.com)
  • However, the mechanisms contributing to miR-10b-mediated glioma cell survival and proliferation are unexplored. (oncotarget.com)
  • The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)
  • A proliferative exhausted CD8 + T cell cluster (P-Tex) which was beneficial to survival outcomes of patients with HPV-positive HNSCC was identified. (elifesciences.org)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
  • AKAP12 knockdown in benign meningioma cells SF4433 increases proliferation, cell cycle, migration, invasion, and confers an anaplastic profile. (nature.com)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • CRC cell lines were transiently transfected with siRNAs targeting UHRF1 , after which DNA methylation was analyzed using dot blots, bisulfite pyrosequencing, and Infinium HumanMethylation450 BeadChip assays. (biomedcentral.com)
  • E2F1 mRNA expression was also evaluated by RT-qPCR in the melanoma cell line, SK MEL 267, before and after exposure to heat stress. (biomedcentral.com)
  • Our results also suggest that environmental insults, such as heat stress, could contribute to an aberrant E2F1 activity by inducing E2F1 mRNA expression. (biomedcentral.com)
  • We observed a statistically significant increased messenger RNA (mRNA) expression in E2F1/3/5/7 compared with matched normal tissues. (fortunepublish.com)
  • The action of HMGB1 on the MDM2 promoter is in a p53-dependent manner whereas its activation of the E2F1 promoter is p53-independent. (edu.hk)
  • To study the cellular function of Ecd in mammalian cells, we generated Ecd(lox/lox) mouse embryonic fibroblast cells from Ecd floxed mouse embryos. (unmc.edu)
  • The use of hASCs for therapeutic applications has grown substantially in the last years, because the use of stem cells from adult tissues circumvent some ethical issues associated with the application of embryonic stem cells, and because of their accessibility via isolation from lipoaspirates, a disposable byproduct of cosmetic surgery. (biomedcentral.com)
  • Recent studies have demonstrated the existence of multiple copies of E2F1 gene in melanoma specimens which could explain the deregulated E2F1 activity in this type of cancer. (biomedcentral.com)
  • Since germline copy number variations (CNVs) have been associated with increased susceptibility to different types of cancer, the aim of our study was to assess germline E2F1 CNV in melanoma patients. (biomedcentral.com)
  • We found that patients diagnosed with melanoma (1.6%, 9/552) harboured frequently altered germline E2F1 copies compared to healthy subjects (0%, 0/520). (biomedcentral.com)
  • This is the first study that shows a relation between germline E2F1 CNV and melanoma, suggesting that altered copies of this gene might be a predisposing factor to skin cancer. (biomedcentral.com)
  • Therefore, subjects with multiple constitutive copies of E2F1 are at greater risk of developing melanoma when exposed to heat. (biomedcentral.com)
  • Melanoma is the most deadly form of skin cancer that arises from uncontrolled proliferation of melanocytes. (biomedcentral.com)
  • Given the pivotal role that E2F1 has in cell proliferation, its involvement in melanoma growth and progression is not surprising. (biomedcentral.com)
  • Blocking lactate generation in metformin-treated melanoma cells results in diminished cell proliferation and tumor progression in mice. (dcaguide.org)
  • Conversely, it has also been reported that melanoma cells display increased OXPHOS function which causes drug resistance [ 19 - 21 ] . (dcaguide.org)
  • Owing to the metabolic flexibility in choosing various metabolic pathways, particularly glutamine metabolism [22] , melanoma cells can escape from perturbation of a molecule in a metabolic pathway [23] . (dcaguide.org)
  • Skin Cutaneous melanoma (SKCM) is a highly aggressive malignant neoplasm that develops from uncontrolled proliferation and transformation of melanocytes. (fortunepublish.com)
  • Furthermore, the cell expansion poses a risk of accumulating genetic abnormalities that could lead to malignant cell transformation. (biomedcentral.com)
  • Background Renal cell carcinomas (CCR) account for 1%C3% of all malignant visceral neoplasms and 90% of renal tumors. (irjs.info)
  • These data identify new mechanisms by which the cellular response to stress is differentially controlled at different stages of the cell cycle. (edgehill.ac.uk)
  • The anatomopathological study of CDX4 the gallbladder was appropriate for infiltrating metastasis from clear-cell carcinoma of principal renal site. (irjs.info)
  • AZD6738 biological activity RCC, renal cell carcinomas strong class="kwd-title" Keywords: Gallblader tumor, Renal cell carcinomas, Latente metastasis, Case report 1. (irjs.info)
  • It was discovered when pulmonary fibroblasts slowed down their proliferation and eventually ended in cell death after cell passaging had been performed for more than 8 months. (dojindo.com)
  • SNHG29 functions as a tumor suppressor, significantly inhibiting FLT3-ITD AML cell proliferation and decreasing sensitivity to cytarabine in vitro and in vivo models. (bvsalud.org)
  • The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
  • Herein, DNA microarray analysis was conducted to investigate the common mechanisms responsible for the antitumor effects of butyric acid, isobutyric acid and acetic acid on human colorectal carcinoma cells and identified candidate signal transduction pathways using the Ingenuity Pathway Analysis (IPA) software. (iiarjournals.org)
  • Since this is a highly sought after clinical goal, we are elucidating the mechanisms of immune cell recruitment so they can be targeted to kill cancer cells. (umn.edu)
  • These mechanisms can malfunction in pediatric retinoblastoma, and may be harnessed during retinal regeneration to produce new retinal cell types after they've been lost. (umn.edu)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • Eukaryotic cells. (edu.hk)
  • Considering that heat stress has been hypothesised as a contributing factor to skin cancer, we also investigated the effect of heat stress on E2F1 expression. (biomedcentral.com)
  • Many aspects of Hes expression are regulated by Notch signaling, which mediates cell-cell communication. (biologists.com)
  • Ki-67 expression and the proliferation kinetics were investigated. (biomedcentral.com)
  • Interestingly, targeting WDR5 by siRNA and OICR-9429 could block IFN-γ-induced PD-L1 expression in PCa cells. (thno.org)
  • EIF5B promotes HCC proliferation and invasion by increasing ASAP1 expression [ 5 ]. (techscience.com)
  • In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
  • S transition in the cell cycle, upregulated expression of tumor-promoting microenvironmental factors, and profound dysregulation of metabolic pathways (e.g., increased aerobic glycolysis, downregulation of pathways that metabolize drugs and xenobiotics). (biomedcentral.com)
  • In this study, an anti-sense strategy was used to suppress the HMGB1 expression level in the human breast cancer MCF-7 cell line. (edu.hk)
  • This indicates the importance of HMGB1 in p53 expression and cell cycle checkpoint control. (edu.hk)
  • In fact, miR-221 is over-expressed in more than 70% of hepatocellular carcinomas, as well as in other human neoplasms, and it can stimulate cell proliferation by inhibiting the expression of the cyclin-dependent kinase inhibitors p27 and p57. (unife.it)
  • As a result, changes in SA-ß-Gal expression, cell cycle progression, and mitochondrial membrane potential were observed. (dojindo.com)
  • Common detection indicators for assessing cellular senescence include features related to cell cycle progression (DNA synthesis, p16/p21 expression, etc.), features related to morphology (of the cell, nucleus, nucleolus, etc. (dojindo.com)
  • Further, sodium butyrate has been shown to influence the adhesion and growth-regulating galectin network that controls the proliferation of human colon cancer cells in vitro ( 10 ). (iiarjournals.org)
  • Cell culture and in vitro assessment of the inhibitory effects of butyric acid, isobutyric acid and acetic acid on the proliferation of a human colorectal carcinoma cell line. (iiarjournals.org)
  • GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
  • In contrast, cyclin A binding to E2F-1 impedes PRMT1 methylation and augments PRMT5 methylation, thus ensuring that E2F-1 is locked into its cell-cycle progression mode. (embl.de)
  • Rb1 and Pten Co-Deletion in Osteoblast Precursor Cells Causes Rapid Lipoma Formation in Mice. (umn.edu)
  • Co-deleting Pten with Rb in retinal progenitor cells in mice results in fully penetrant bilateral retinoblastomas. (umn.edu)
  • GC B cells of Cdkn1a Ezh2 mice have high levels of phospho-Rb, indicating that loss of Cdkn1a enables progression of cell cycle. (cornell.edu)
  • RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)
  • Using a 3D B cell follicular organoid system that mimics the GC reaction, we show that depletion of EZH2 suppresses G1 to S phase transition of GC B cells in a Cdkn1a-dependent manner. (cornell.edu)
  • This strongly supports the amplification of E2F1 gene in predisposing to cancer. (biomedcentral.com)
  • We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
  • The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. (innatedb.com)
  • In S-phase, the NF-κB response was delayed or repressed, while cell cycle progression was unimpeded. (edgehill.ac.uk)
  • We previously found that SCFAs, such as butyric acid, isobutyric acid and acetic acid, inhibit the growth of cultured human colorectal cancer cells and that butyric acid is the strongest inhibitor ( 3 ). (iiarjournals.org)
  • The human colorectal carcinoma cell line (DLD-1 cells) was purchased from the Riken BRC Cell Bank, Japan. (iiarjournals.org)
  • Inactivation of Rb may contribute to many human malignancies including familial retinoblastoma, small-cell lung carcinomas, cervical carcinomas, prostate carcinomas, breast carcinomas, and some forms of leukaemias ( Burkhart,2008 ). (eu.org)
  • Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
  • We performed cell-level multi-omics sequencing on human HNSCC samples to decipher the multi-dimensional characteristics of Tex cells. (elifesciences.org)
  • Human adipose tissue-derived stem cells (hASCs) are attractive cells for therapeutic applications and are currently being evaluated in multiple clinical trials. (biomedcentral.com)
  • B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)