Loading...
  • cellular
  • The N-terminal region of the adenovirus (Ad) 12S E1A gene product targets several cellular proteins that are essential for the induction of S phase, cellular immortalization, cellular transformation, transcriptional repression, and transcriptional activation. (asm.org)
  • The protein products of 12S and 13S differ only in the presence of conserved region 3 (CR3), which functions to transactivate a number of cellular and viral genes ( 22 ). (asm.org)
  • AdE1A can cooperate with AdE1B or activated ras genes to transform both human ( 6 ) and rodent ( 33 ) cells by targeting a limited number of cellular proteins through the N-terminal region, CR1, CR2, and CR4 ( 3 , 18 , 26 , 44 ). (asm.org)
  • Interestingly, however, the AdE1A N-terminal regions from different Ad serotypes appear to bind to a similar set of cellular proteins, presumably to perform similar functions during infection and transformation ( 3 , 18 ). (asm.org)
  • adenovirus
  • Specifically inhibited by the adenovirus oncoprotein E1A. (mybiosource.com)
  • Adenovirus (Ad) E1A expression is essential for Ad replication and Ad-mediated transformation ( 34 ). (asm.org)
  • lysine
  • Comparison of our dataset with three previous global acetylomic studies uniquely revealed 53 lysine-acetylated proteins. (plos.org)
  • acetylation
  • In the years since, families of histone acetyltransferases and deacetylases have been discovered, and nuclear protein acetylation has emerged as paramount in chromatin remodeling and transcriptional regulation . (plos.org)
  • Caloric restriction in mice is cardioprotective and leads to diminished acetylation of mitochondrial proteins, which in turn, correlates with reduced ROS production from the electron transport chain . (plos.org)
  • phosphorylation
  • A central regulatory mechanism of FOXO proteins is phosphorylation by the serine-threonine kinase Akt/protein kinase B (Akt/PKB), downstream of phosphatidylinositol 3-kinase (PI3K), in response to insulin or several growth factors. (genome.jp)
  • Phosphorylation at three conserved residues results in the export of FOXO proteins from the nucleus to the cytoplasm, thereby decreasing expression of FOXO target genes. (genome.jp)
  • NOR1 deficiency alters phosphorylation of the retinoblastoma protein by preventing mitogen-induced cyclin D1 and D2 expression. (ahajournals.org)
  • The majority of the acetyl-proteins are enzymes involved in fatty acid metabolism, oxidative phosphorylation or the TCA cycle. (plos.org)
  • CtBP
  • Exon 2, encompassing the whole C-terminal region, suppresses AdE1A/ ras -mediated transformation, primarily through a conserved PXDLS motif that targets the transcriptional corepressor molecule CtBP, and deletion of the PXDLS motif enhances E1A/ ras -mediated transformation ( 4 ). (asm.org)
  • In contrast, the C-terminal region enhances E1A/E1B-mediated transformation ( 14 ) through the targeting of AdE1A to the nucleus and the binding of CtBP. (asm.org)
  • In the nucleus, DNA-binding proteins of the T-cell factor (Tcf) and lymphoid enhancer-binding factor 1 (Lef-1) family are bound by transcriptional repressors such as transducin-like enhancer proteins (Tle) and C-terminal binding protein (CtBP). (aacrjournals.org)
  • residues
  • We determined that although common residues within the N-terminal region can form partial binding sites for these proteins, point mutants were also generated that could discriminate between binding sites. (asm.org)
  • amino acids
  • We therefore undertook an extensive site-directed mutagenesis approach to generate specific point mutants and to precisely map the binding sites for CBP, p300, TATA-binding protein (TBP), S4, S8, hGcn5, P/CAF, and Ran within the first 30 amino acids of the Ad5 12S E1A protein. (asm.org)
  • The average coverage of the annotated isotigs was x40 containing 5655 unique gene IDs and 785 full-length cDNAs coding for proteins containing 58-1536 amino acids. (biomedcentral.com)