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ConjugatesFluorescent dyeSynthesisChemFluoresceinCyanineSpectraConjugationPhotochemicalFluorophorePolymersOligonucleotideExtinction coefficientPigmentsReagentsConjugateMoleculesIRDye800CWPhotostabilityWidelyPeptideBiologicalEmissionRhodaminesSyntheticConventionalProteinColorsAbstractCellsApplicationsAcidGreenChemicalStructureLaserWaterUnique
Conjugates4
  • 1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols 1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. (vectorlabs.com)
  • Bioconjugates are generally classified as either direct or indirect conjugates. (justia.com)
  • iFluor® 440 dye conjugates are significantly brighter than the corresponding bioconjugates of DEAC or other spectrally similar dyes (such as SpectrumAqua). (aatbio.com)
  • US Patent #6,348,317 - Fluorescent and DNA cleavage properties of peptide/dye conjugates. (mtu.edu)
Fluorescent dye2
  • 800CW dye-conjugated agents and probes are currently propelling more than a dozen Phase I or Phase II clinical trials, more than any other near-infrared fluorescent dye based on its enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios. (broadpharm.com)
  • The function of these protein tags, such as the HaloTag® or the SNAP or CLIP tag , is based on the recognition of a particular substrate or ligand (e.g. fluorescence conjugate of an oligonucleotide and a fluorescent dye). (biomers.net)
Synthesis4
  • Functionalization of the meso-phenyl ring of rhodamine dyes through SNAr with sulfur nucleophiles: Synthesis, biophysical characterizations, and comprehensive NMR analysis. (mpg.de)
  • Masked rhodamine dyes of five principal colors revealed by photolysis of a 2-diazo-1-indanone caging group: synthesis, photophysics, and light microscopy applications. (mpg.de)
  • Polar red-emitting rhodamine dyes with reactive groups: Synthesis, photophysical properties, and two-color STED nanoscopy applications. (mpg.de)
  • Phthalocyanines dyes can be produced with solid-state synthesis instead of high-boiling organic solvents. (aalto.fi)
Chem2
Fluorescein2
  • Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. (wikipedia.org)
  • Carborhodol: A new hybrid fluorophore obtained by combination of fluorescein and carbopyronine dye cores. (mpg.de)
Cyanine9
  • The main application for cyanine dyes is in biological labeling. (wikipedia.org)
  • For applications to biotechnology, special cyanine dyes are synthesized from 2, 3, 5 or 7-methine structures with reactive groups on either one or both of the nitrogen ends so that they can be chemically linked to either nucleic acids or protein molecules. (wikipedia.org)
  • Cyanine dyes are available with different modifications such as methyl, ethyl or butyl substituents, carboxyl, acetylmethoxy, and sulfo groups which alter their hydrophilicity. (wikipedia.org)
  • Other cyanine dyes are useful: Cy3.5 can replace sulfoRhodamine 101. (wikipedia.org)
  • Sulfo-Cyanine dyes bear one or two sulfo groups, rendering the Cy dye water-soluble, but tri- and quadri-sulfonated forms are available for even higher water solubility. (wikipedia.org)
  • AZDye™ 800 is a conceptually new class of sterically shielded NIR cyanine heptamethine dye. (vectorlabs.com)
  • Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800 . (vectorlabs.com)
  • In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory. (vectorlabs.com)
  • Fine Structure in Electronic Spectra of Cyanine Dyes: Are Sub-Bands Largely Determined by a Dominant Vibration or a Collection of Singly Excited Vibrations? (few.de)
Spectra3
  • 4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent. (vectorlabs.com)
  • Holmes, W. C. (1930) The absorption spectra of dyes. (photochemcad.com)
  • Chen, R. F. and C. H. Scott (1985) Atlas of fluorescence spectra and lifetimes of dyes attached to protein. (photochemcad.com)
Conjugation2
  • Filling the Green Gap of a Megadalton Photosystem I Complex by Conjugation of Organic Dyes. (mpg.de)
  • The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of bifunctional boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. (justia.com)
Photochemical2
  • These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability. (vectorlabs.com)
  • Chan, M. S. and J. R. Bolton (1980) Structures, reduction potentials and absorption maxima of synthetic dyes of interest in photochemical solar-energy storage studies. (photochemcad.com)
Fluorophore3
  • Cy5 became a popular replacement for far red fluorescent dyes because of its high extinction coefficient (as small as 1 nanomol can be detected in gel electrophoresis by naked eye) and its fluorophore emission maximum in the red region, where many CCD detectors have maximum sensitivity and biological objects give low background interference. (wikipedia.org)
  • While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (vectorlabs.com)
  • TAMRA dye - Carboxy tetramethyl-rhodamine (TAMRA) has been a widely used fluorophore for preparing bioconjugates. (axispharm.com)
Polymers2
  • Dyes aside, Muellen's contributions to polymer chemistry range from the invention of new approaches for building optoelectronic polymers to the development of materials for lithium and hydrogen storage. (acs.org)
  • His group has also figured out how to elegantly build various aromatic polymers, nanocomposites, and bioconjugates. (acs.org)
Oligonucleotide2
  • 5-TAMRA has achieved prominence as a dye for oligonucleotide labeling and automated DNA sequencing applications. (axispharm.com)
  • Non-nucleoside phosphoramidites of xanthene dyes (FAM, JOE, and TAMRA) for oligonucleotide labeling. (aatbio.com)
Extinction coefficient1
  • Due to its high molar extinction coefficient, this dye is also easily detected by naked eye on electrophoresis gels, and in solution. (wikipedia.org)
Pigments1
  • Zollinger, H. (1987) Color Chemistry: Syntheses, Properties, and Applications of Organic Dyes and Pigments. (photochemcad.com)
Reagents1
Conjugate1
  • PEGylation is another modification that confers hydrophilicity, not only to the dye but also to the labeled conjugate. (wikipedia.org)
Molecules2
  • 3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. (vectorlabs.com)
  • Seeing the vivid colors of azo dyes appear as he synthesized molecules during high school lab sessions inspired Klaus Muellen, 64, to study chemistry at university. (acs.org)
IRDye1
800CW1
  • 800CW dye can be conjugated to primary antibody, secondary antibody, or other biomolecules for the application below. (broadpharm.com)
Photostability1
  • In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability. (vectorlabs.com)
Widely1
  • 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C 18 carbon) hydrocarbon chains into the lipid bilayer. (biotium.com)
Peptide1
  • Thompson, M. (2007) Spectral Properties and DNA Targeting Features of a Thiazole Orange- Peptide Bioconjugate. (mtu.edu)
Biological2
  • MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces. (vectorlabs.com)
  • Results show the utility of these dyes in studying biological processes. (optica.org)
Emission2
  • Cy5.5 is a near-infrared (IR) fluorescence-emitting dye (excitation/emission maximum 678/694 nm). (wikipedia.org)
  • 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) is a lipophilic carbocyanine dye similar to DiI, but with longer absorption and emission wavelengths. (biotium.com)
Rhodamines2
  • Far-red emitting fluorescent dyes for optical nanoscopy: Fluorinated silicon-rhodamines (SiRF dyes) and phosphorylated oxazines. (mpg.de)
  • Red-emitting rhodamines with hydroxylated, sulfonated, and phosphorylated dye residues and their use in fluorescence nanoscopy. (mpg.de)
Synthetic1
  • Cyanines, also referred to as tetramethylindo(di)-carbocyanines are a synthetic dye family belonging to the polymethine group. (wikipedia.org)
Conventional1
  • The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. (aatbio.com)
Protein1
  • Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system, in which, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium Streptomyces avidinii), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. (justia.com)
Colors1
  • MemBrite™ Fix dyes covalently label cell surface proteins, and are available in with a wide selection of bright and photostable colors. (biotium.com)
Abstract1
  • In this book changing ways of death in ABSTRACT the samples" of malware an, top samples" and 2nd Tibet academia Melvyn C. Goldstein is a similar and transient thought of the dye and a technique for the level. (skiclub-todtmoos.de)
Cells5
  • Efficient two-photon (2PA) absorbing dyes and bioconjugates were used in two-photon fluorescence microscopy (2PFM) of cells, tissue sections, and excised tumors. (optica.org)
  • CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. (biotium.com)
  • We also offer classic membrane and membrane potential dyes, including CellBrite™ and CellBrite™ NIR dyes , which are lipophilic carbocyanine dyes that stain the plasma membrane of cells and are non-toxic for long-term tracking. (biotium.com)
  • And she is involved in the development of AU-011 (Aura Biosciences), which consists of viral-like particle bioconjugates that bind selectively to receptors on cancer cells. (medscape.com)
  • Finding the Way to Solar Fuels with Dye-Sensitized Photoelectrosynthesis Cells. (acs.org)
Applications1
  • It is used in in vivo imaging applications, as well as the Cy7.5 dye. (wikipedia.org)
Acid1
  • 1. Chemical modification of hyaluronic acid by carbodiimides, Bioconjugate chemistry, 1991, 2(4):232-41. (creativepegworks.com)
Green2
  • DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. (biotium.com)
  • A spectrally unique, 405 nm-excitable green dye. (biotium.com)
Chemical1
  • Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity. (vectorlabs.com)
Structure1
  • Impact of Photosensitizing Multilayered Structure on Ruthenium(II)-Dye-Sensitized TiO2-Nanoparticle Photocatalysts. (acs.org)
Laser2
Water1
  • iFluor® 440 dyes have excellent water solubility and are designed to be a superior replacement for DEAC dye. (aatbio.com)
Unique1
  • Biotium offers two new classes of dyes for covalent labeling of the cell surface that are unique in that they can withstand fixation and permeabilization for subsequent IF staining. (biotium.com)