Loading...
  • biotin-dUTP
  • The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. (biotium.com)
  • In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. (biotium.com)
  • We also examined the expression of PCNA and the extent of denucleation using terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick-end labeling (TUNEL) of lentoids (lens fiber-like cells) during chick lens epithelial cell differentiation in vitro. (molvis.org)
  • This profile of expression correlated with the events in chick lens epithelial cell cultures: i) increased Survivin expression was associated with an increase in PCNA staining up to day 6 of culture and ii) downregulation of Survivin expression at day 8 of culture was coincident with a dramatic decrease in PCNA staining and an increase in TdT-mediated biotin-dUTP nick-end labeling in lentoids. (molvis.org)
  • dTTP
  • dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. (anacrom.es)
  • synthesize
  • Cyanine 555-dUTP and Cyanine 647-dUTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. (biotium.com)
  • Digoxigenin-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. (biotium.com)
  • terminal
  • Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3'-end terminal labeling. (biotium.com)
  • The photoreceptor loss and apoptosis were evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling in 3-week-old rd10/hTf mice and compared with 3-week-old rd10 mice. (molvis.org)
  • method
  • This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP. (biotium.com)
  • We modified the Illumina TruSeq RNA sample preparation by implementing the strand specificity feature using the dUTP method. (nih.gov)