Dual specificity protein kinases that activate. Medical search. Frequent questions

Background: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. (hatinhibitor.com)
Applications: Dual specificity protein phosphatase homolog hMKP-R antibody can be used for the detection of Dual specificity protein phosphatase homolog hMKP-R by Western Blot. (hatinhibitor.com)
Dual specificity protein phosphatase 19 is an enzyme that in humans is encoded by the DUSP19 gene. (wikipedia.org)
A novel dual specificity phosphatase SKRP1 interacts with the MAPK kinase MKK7 and inactivates the JNK MAPK pathway. (wikipedia.org)
Overview of all the structural information available in the PDB for UniProt: Q8WTR2 (Human Dual specificity protein phosphatase 19 (DUSP19)) at the PDBe-KB. (wikipedia.org)
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. (antibodies-online.com)
Find Dual Specificity Phosphatase 3 Antibodies for a variety of species such as anti-Human Dual Specificity Phosphatase 3, anti-Mouse Dual Specificity Phosphatase 3, anti-Rat Dual Specificity Phosphatase 3. (antibodies-online.com)
Find Dual Specificity Phosphatase 3 Antibodies validated for a specific application such as WB, ELISA, IHC, IF (cc). (antibodies-online.com)
Find Dual Specificity Phosphatase 3 Antibodies with a specific Host. (antibodies-online.com)
Find available monoclonal or polyclonal Dual Specificity Phosphatase 3 Antibodies. (antibodies-online.com)
Find Dual Specificity Phosphatase 3 Antibodies with a specific conjugate such as Biotin, FITC, HRP. (antibodies-online.com)
Dual Specificity Phosphatase 26 (DUSP26) inhibits MAP kinase p38 by dephosphorylating it and inhibits p38-mediated apoptosis in anaplastic thyroid cancer cells. (prospecbio.com)
The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. (embl.de)
Analysis of the predicted amino acid sequence suggests that the protein consists of an active phosphatase domain, an inactive phosphatase-like domain, and an NH2-terminal extension. (embl.de)
Mutation of the catalytic cysteinyl residue in the active phosphatase domain reduced the in vitro activity of the mutant protein to less than 0.5% of wild type activity, while mutation of the corresponding cysteinyl residue of the inactive phosphatase-like domain had no effect on in vitro activity. (embl.de)
Genes that are upregulated by GC treatment, such as dual-specificity phosphatase 1 (DUSP1), GC-induced leucine zipper (GILZ), and interleukin (IL)-10, are highly immunosuppressive and contribute to the overall effect of GC treatment ( 1 - 3 ). (frontiersin.org)
Mitogen-activated protein kinase phosphatases (MKPs) belongs to the dual-specificity phosphatase (DUSP) family. (cancer-research-network.com)
BCI-215, a dual-specificity phosphatase inhibitor, reduces UVB-induced pigmentation in human skin by activating mitogen-activated protein kinase pathways. (cha.ac.kr)
SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated protein kinase phosphatase - 1 . (pp1a.com)
Activation of mitogen-activated protein kinase phosphatase-1 (MKP-1), dual-specificity protein phosphatase, set the mitogen-activated protein kinase signaling. (pp1a.com)
The pseudophosphatase MK-STYX [MAPK (mitogen-activated protein kinase) -phosphoserine/threonine/tyrosine-interacting protein] is an atypical MKP (MAPK phosphatase). (wm.edu)
The DUSP (dual-specificity phosphatase) domain of MK-STYX lacks critical histidine and cysteine residues in the active site motif (HCX5R), rendering it catalytically inactive. (wm.edu)
Also important to the function of MKPs is the CH2 domain (cell division cycle 25 phosphatase homology 2 domain), which is interrupted by a KIM (kinase-interacting motif). (wm.edu)
Dual specificity phosphatase 3 (DUSP3), a suppressor of multiple protein tyrosine (Tyr) kinases, is decreased in lung cancer tissues. (biomedcentral.com)
Proximity ligation assay, immunoblotting, and phosphatase assay were performed to study the effect of DUSP3 on the TJ protein occludin (OCLN). (biomedcentral.com)
DUSP3/VHR, related to the vaccinia virus phosphatase VH1, is initially found to be a phosphatase that dephosphorylates multiple protein tyrosine kinases (PTKs) in vitro [ 21 ]. (biomedcentral.com)
Glucocorticoids induce rapid up-regulation of mitogen-activated protein kinase phosphatase-1 and dephosphorylation of extracellular signal-regulated kinase and impair proliferation in human and mouse osteoblast cell lines. (ox.ac.uk)
Phosphatase activity was up-regulated by Dex treatment, and inhibition of ERK activity by Dex was also reversed by the protein tyrosine phosphatase inhibitor, vanadate. (ox.ac.uk)
Coupled with the rapidity of Dex action, this indicates immediate-early gene phosphatase involvement, and we therefore used quantitative, real-time PCR to examine expression profiles of the dual-specificity MAPK phosphatases, MKP-1 and MKP-3. (ox.ac.uk)
A CALCIUM and CALMODULIN-dependent serine/threonine protein phosphatase that is composed of the calcineurin A catalytic subunit and the calcineurin B regulatory subunit. (bvsalud.org)

Dual-specificity mitogen-activated protein kinases kinases (MAPKKs) are the immediate upstream activators of MAPKs. (edu.sa)

Mitogen-activated protein kinase (MAPK) cascades transmit environmental signals and induce stress and defence responses in plants. (biorxiv.org)
They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. (antibodies-online.com)
Activation occurs through phosphorylation at Ser 271 and Thr 275 by several upstream MAPK kinase kinases (MAP3Ks). (rndsystems.com)
Activation of the pheromone receptor-coupled G protein initiates a four-tiered cascade of protein kinases, ultimately resulting in stimulation of a messenger-activated protein kinase or MAPK (Fus3) that translocates into the nucleus. (berkeley.edu)
STE7 (also known as MAPKK, MAP2K or MEK) kinases are part of the MAPK signalling cascades. (guidetoimmunopharmacology.org)
They are activated by phosphorylation by upstream STE11 (MAP3K, MAPKKK) kinases and phosphorylate downstream MAPK kinases. (guidetoimmunopharmacology.org)
Mitogen-activated protein kinase kinase (MAPKK) is a dual specificity protein kinase that exhibits a high degree of specificity toward its downstream target, mitogen-activated protein kinase (MAPK). (tau.ac.il)
The pathways involve a triple kinase core cascade comprising of the MAP kinase (MAPK), which is phosphorylated and activated by a MAPK kinase (MAPKK or MKK), which itself is phosphorylated and activated by a MAPK kinase kinase (MAPKKK or MKKK). (umbc.edu)
PBS2 and Wis1 are components of stress-activated MAPK cascades in budding and fission yeast, respectively. (umbc.edu)
Wis1 phosphorylates and activates the MAPK Sty1 (also called Spc1 or Phh1), which stimulates a transcriptional response to a wide range of cellular insults through the bZip transcription factors Atf1, Pcr1, and Pap1. (umbc.edu)
We find that ERK activity is essential for serum-induced osteoblast proliferation in vitro because inhibition of MAPK/ERK kinase activity by U0126 completely abolished both serum-induced activation of ERK and proliferation of mouse (MBA-15.4) and human (MG-63) osteoblast cell lines. (ox.ac.uk)
This protein is part of a signaling pathway called the RAS/MAPK pathway, which transmits chemical signals from outside the cell to the cell's nucleus. (medlineplus.gov)
The genetic changes abnormally activate the protein, which disrupts the tightly regulated RAS/MAPK signaling pathway in many types of cells. (medlineplus.gov)
The mutations lead to the production of a version of the protein that is overactive, which increases RAS/MAPK signaling in bone tissue. (medlineplus.gov)

These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. (hatinhibitor.com)
Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. (antibodies-online.com)
Tyrosine specific protein phosphatases (PTPases) contain two conserved cysteines, the second one has been shown to be absolutely required for activity. (embl.de)
This domain can be found in dual specificity phosphatases. (embl.de)
The deduced amino acid sequence of PPS1p shows similarity with protein-tyrosine phosphatases (PTPases) and is most closely related to a subfamily of PTPases that are capable of dephosphorylating phosphoseryl and phosphothreonyl residues as well as phosphotyrosyl residues. (embl.de)
and, various classes of phosphotyrosine-directed, phosphoserine- / phosphothreonine-directed, and dual-specificity phosphoprotein phosphatases that dephosphorylate activated MAPKs. (berkeley.edu)

We have shown that Ypk1, a member of the AGC class of protein kinases conserved from yeast to humans, is the essential target of and activated via phosphorylation by the plasma membrane-associated TORC2 complex. (berkeley.edu)
The course of nucleocytoplasmic shuttling of SIMK correlated temporally with the dual phosphorylation of the pTEpY motif. (edu.sa)
Expression levels of PER2, a clock protein, were elevated in Sik3 -knockdown cells but down-regulated in Sik3 -overexpressing cells, which could be attributed to a phosphorylation-dependent decrease in PER2 protein stability. (elifesciences.org)
DUSP3 regulated OCLN ubiquitination and degradation through decreasing OCLN tyrosine phosphorylation directly or through suppressing focal adhesion kinase, the OCLN kinase. (biomedcentral.com)
ATAD2 straight interacts with MKK3 and MKK6 two dual-specificity proteins kinases that activate p38 phosphorylation MKK3 and MKK6 are two main factors that control p38 phosphorylation. (bio2009.org)

DUSP-MKPs dephosphorylate and inactivate the MAPKs extracellular signal-regulated kinase (ERK), JNK/SAPK, and p38 on tyrosine and threonine residues. (cancer-research-network.com)

In response to mechanical stress, VEGF gene and protein expression increased in patient AVM endothelial cells. (researchgate.net)
Bevacizumab inhibited circular network formation of endothelial AVM cells and lowered VEGF gene and protein expression, even though the cells were exposed to mechanical stress. (researchgate.net)
The duality of this product lies in it's dual specificity to the gene, pH or protein. (reageno.com)
Description of the protein which includes the UniProt Function and the NCBI Gene Summary. (nih.gov)
According to weighted gene co-expression network analysis, pathways positively related to adipogenic differentiation are significantly activated at the differentiation stage, while WNT, FOXO and other pathways that inhibit preadipocyte differentiation are negatively regulated. (biomedcentral.com)
C-met is a protein encoded by a met gene in humans, which has tyrosine kinase activity. (cancer-research-network.com)
The MAP2K1 gene provides instructions for making a protein known as MEK1 protein kinase. (medlineplus.gov)
The identified MAP2K1 gene mutation is a germline mutation that replaces the amino acid glutamic acid with the amino acid glycine at position 102 (written as Glu102Gly or E102G) in MEK1 protein kinase. (medlineplus.gov)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

Overexpression of mitogen-activated protein kinase kinase (MAPKK) and its mutants in NIH 3T3 cells. (tau.ac.il)
Protein kinases (PKs), MAP kinase kinase (MAPKK) subfamily, fungal PBS2-like proteins, catalytic (c) domain. (umbc.edu)
The MAPKK subfamily is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine kinases, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. (umbc.edu)

However, the KEGG signalling pathways namely "tumour necrosis factor (TNF)" and "cytokine-cytokine receptor interaction" were downregulated at 24H but activated at 48H. (nature.com)
Cancer cells survive by co-opting intracellular growth pathways regulated through kinase signaling. (escholarship.org)
Many kinases in these pathways are validated drug targets and kinase inhibitors are first-line treatment for several advanced cancers. (escholarship.org)
At the proliferation stage, proliferation related pathways and basic cellular and metabolic processes were inhibited, while regulatory factors that initiate differentiation enter the ready-to-activate state, which provides a precondition for initiating adipose differentiation. (biomedcentral.com)
Our outcomes suggested that additional substitute apoptotic pathways may be activated in HCC cells with mutant p53. (bio2009.org)
The mitogen-activated protein (MAP) kinase signaling pathways are important mediators of cellular responses to extracellular signals. (umbc.edu)
Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)

Our results suggest that the combined use of Roscovitine and Kenpaullone may be useful for identifying substrates and physiological roles of cyclin-dependent protein kinases, whereas the combined use of Kenpaullone and LiCl may be useful for identifying substrates and physiological roles of glycogen synthase kinase 3. (nih.gov)
Risk factors for depression initiate an infection-like inflammation in the brain that involves activation microglial Toll-like receptors and glycogen synthase kinase-3β (GSK3β). (mdpi.com)

Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. (affbiotech.cn)

Rho-associated protein kinases (ROCKs) are serine-threonine kinases contain isoforms ROCK1 and ROCK2. (cancer-research-network.com)

MEK1 protein kinase appears to be essential for normal development before birth and for survival after birth. (medlineplus.gov)
Each mutation changes a single protein building block (amino acid) in MEK1 protein kinase. (medlineplus.gov)
The known mutations change single amino acids in MEK1 protein kinase. (medlineplus.gov)

Here, this specific problem is addressed by molecular studies of the human NMP kinase AK3, which uses GTP to phosphorylate AMP. (scilifelab.se)

The PPS1 protein was expressed in Escherichia coli, and the protein was shown to catalyze the hydrolysis of p-nitrophenyl phosphate, dephosphorylate phosphotyrosyl, and phosphothreonyl residues in synthetic diphosphorylated peptides and to inactivate the human ERK1 protein. (embl.de)
PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine or tyrosine residues on protein substrates. (umbc.edu)
Here, molecular dynamics simulations, enzyme kinetics, X-ray crystallography, and nuclear magnetic resonance spectroscopy are combined to elucidate the catalytic mechanism of adenylate kinase and to delineate the roles of catalytic residues in catalysis and the conformational change in the enzyme. (scilifelab.se)
The results illustrate a linkage between enzymatic catalysis and collective protein motions, whereby the disparate time scales between the two processes are bridged by a cascade of intermediate-scale motion of catalytic residues modulating the free energy landscapes of the catalytic and conformational change processes. (scilifelab.se)

We have previously examined the specificities of 28 commercially available compounds, reported to be relatively selective inhibitors of particular serine/threonine-specific protein kinases [Davies, Reddy, Caivano and Cohen (2000) Biochem. (nih.gov)
The first generation of kinase inhibitors were intended to target single proteins. (escholarship.org)
However, the highly conserved active sites of protein kinases prohibited the design of perfectly selective inhibitors. (escholarship.org)
Unexpectedly, this specificity problem led to the development of clinically useful, multi-targeted kinase inhibitors. (escholarship.org)
The design of dual PI3K/PTK inhibitors is chemically challenging because PI3Ks and PTKs are members of significantly divergent kinase families. (escholarship.org)
Selective compounds have been developed that target either the extracellular ligand-binding region of the EGFR (including a number of monoclonal antibodies [MAbs], immunotoxins, and ligand-binding cytotoxic agents) or the intracellular tyrosine kinase region (including various small-molecule inhibitors). (medscape.com)

This domain occurred 388 times on human genes ( 899 proteins). (umbc.edu)

TJs, tightly fused regions between the outer membrane of adjacent cells, are formed by integral transmembrane proteins such as OCLN and claudins, anchoring proteins such as zonula occludens (ZO)1-3, and other TJ-associated/regulatory proteins [ 4 , 5 ]. (biomedcentral.com)
The HER (erbB) family of transmembrane receptor tyrosine kinases is one of the cytostatic targets in tumor cell growth and survival. (medscape.com)

In addition, at 48H, BPs linked to inflammatory phenotypes were activated (confirming KEGG results), while BPs linked to extracellular matrix (ECM) structural organisation, endothelial cell proliferation, and collagen metabolism were repressed. (nature.com)

Mitogen-activated protein kinase kinase 7 (MKK7 or MAP2K7), also known as Jun kinase kinase 2 (JNKK2), is activated by proinflammatory cytokines and environmental stresses. (rndsystems.com)
MKK7 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr. (rndsystems.com)

Some of the targets of the Liver Failure pipeline drugs market are Caspase, Dual Specificity Mitogen Activated Protein Kinase Kinase 4, Toll Like Receptor 4, Tumor Necrosis Factor, and Androgen Receptor among others. (globaldata.com)
The activation of FMS like receptor tyrosine kinase 3 (FLT3) is a cytokine receptor that belongs to the receptor tyrosine kinase class III. (cancer-research-network.com)
Targeting peroxisome proliferator-activated receptor delta (PPARδ): A medicinal chemistry perspective. (cha.ac.kr)
This inhibition is reversed by cotreatment with the protein synthesis inhibitor, cycloheximide, and by the GC receptor antagonist, RU486, suggesting a classical transcriptional mechanism. (ox.ac.uk)

The ubiquitin-proteasome-system (UPS) is responsible for most of the intracellular protein degradation in eukaryotes. (cancer-research-network.com)
These nonspecific interactions likely act to lower the available intracellular GTP and ATP concentrations and may have driven evolution of the Michaelis constants of NMP kinases accordingly. (scilifelab.se)

We have recently shown that this same pathway modulates the content of integral membrane proteins and, in collaborative studies, that it is also involved in maintenance of the sterol composition of the plasma membrane. (berkeley.edu)
These proteins included catalase, peroxiredoxin, glutathione S-transferase, nucleoside diphosphate kinase 1, endoplasmic reticulum luminal-binding protein 2, and finally plasma membrane aquaporins. (edu.sa)

DUSP26 inactivates MAPK1 and MAPK3 which leads to dephosphorylation of heat shock factor protein 4 and a decrease in its DNA-binding activity. (prospecbio.com)
Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). (nih.gov)
MKP-1 protein was also markedly up-regulated following 1-8 h of Dex treatment, and this correlated precisely with dephosphorylation of ERK. (ox.ac.uk)

DUSP26 also induces activation of MAP kinase p38 and c-Jun N-terminal kinase. (prospecbio.com)
In this regard, we have recently shown that efficient activation of Ypk1 by TORC2 requires stimulation of TORC2 by the GTP-bound state of a Rab5 GTPase (Vps21/Ypt51) and that, in turn, Rab5-dependent endocytosis may be a mechanism to down-regulate TORC2 signaling after it has been activated. (berkeley.edu)
SIMKK-YFP plants showed enhanced activation of Arabidopsis MPK3 and MPK6 kinases upon salt treatment and exhibited high sensitivity against salt stress at the seedling stage, although they were salt insensitive during seed germination. (edu.sa)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

Two kinase families, phosphoinositide-3-kinases (PI3Ks) and protein tyrosine kinases (PTKs), are activated by mutations and amplifications in a disproportionate number of human cancers. (escholarship.org)

While the role of collective protein motions in substrate binding, product release, and regulation of enzymatic activity is generally understood, their roles in catalytic steps per se remain uncertain. (scilifelab.se)
The overall features with specific recognition of the correct substrate and nonproductive binding by the incorrect substrate bear a strong similarity to previous findings for the ATP specific NMP kinase adenylate kinase. (scilifelab.se)
Here, we have discovered an intricate interplay between the enzyme adenylate kinase and its substrate ATP. (scilifelab.se)

Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)

A subset of these compounds potently, and sometimes selectively, inhibit the mammalian target of rapamycin (mTOR), a Ser/Thr kinase in the PI3K family, placing mTOR in kinase space at the intersection of PI3Ks and PTKs. (escholarship.org)

The Wee1 class of protein-tyrosine kinase has an important role in cell cycle control. (berkeley.edu)
We investigated control mechanisms that regulate the activity, localization, and stability of Wee1, especially the bud neck-localized protein kinase Hsl1 and its more distant paralogs (Gin4 and Kcc4), in particular their recruitment to septin filaments, which assemble at the presumptive site of cell division. (berkeley.edu)

Inactivates MAP kinases. (nih.gov)

Proteomic analysis of SIMKK-YFP overexpressors indicated the differential regulation of proteins directly or indirectly involved in salt stress responses. (edu.sa)
Previous reports have suggested that protein kinases play important roles in the regulation of circadian clocks ( Reischl and Kramer, 2011 ). (elifesciences.org)

Salt-inducible kinase 3 (SIK3) plays a crucial role in various aspects of metabolism. (elifesciences.org)

In addition, the presence of EVs reduced inflammatory responses in Pam 3 CSK 4 -treated endothelial cells and HEK Dual reporter cells, demonstrating that TLR2-EVs can act as decoy receptors. (frontiersin.org)
Activated by cytokines and chemokines, leukocytes and endothelial cells express distinct adhesion molecules on their cell surfaces [ 9 ]. (biomedcentral.com)

We also studied the roles of other classes of protein kinases (Cla4) and additional post-translational modifications (SUMOylation) in septin complex assembly, formation of different septin-based supramolecular ensembles, disassembly of septin-containing structures, and the function of septin organization in the events required for cell division and membrane septation during cytokinesis. (berkeley.edu)

Anatomy14

Organisms7

Diseases1

Chemicals and Drugs129

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes40

Information Science3

Health Care1

Phosphatase30

Mitogen-Activat1

MAPK14

Phosphatases6

Phosphorylation5

MAPKs1

Gene9

MAPKK3

Pathways7

Glycogen2

Downstream1

Serine-threoni1

MEK13

Phosphorylate1

Residues4

Inhibitors6

Genes1

Transmembrane2

Extracellular1

MKK72

Receptor4

Intracellular2

Plasma membrane2

Dephosphorylation3

Activation4

Mutations1

Substrate3

Vitro1

MTOR1

WEE12

Inactivates1

Regulation2

SIK31

Endothelial2

Roles1