Dual species biofilms biofilm bacteria biofilms. Medical search. Frequent questions

A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. (frontiersin.org)
However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)
A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis). (lookformedical.com)
26) Previously published research revealed that children with the diagnosis of Autism Spectrum Disease have a microbiome deficient in protective bacteria but also had pathogenic bacteria capable of affecting neurotransmitter levels theoretically necessary for "normal" neural development. (probiorahealth.com)
Co-infection by different pathogenic bacterial species often increases the severity of the underlying condition, and are more difficult to treat [ 1 ]. (biomedcentral.com)
When pathogenic bacteria invade host cells and tissues, the interactions between individual host cells and the bacteria are typically highly variable. (isbscience.org)
In numerous biotic and abiotic situations, pathogenic and saprophytic Leptospira have been observed to produce biofilms. (ijbpr.net)
The pathogenicity and virulence of pathogenic bacteria may be enhanced by biofilm. (ijbpr.net)
The pathogenic bacteria can colonise the host effectively thanks to quorum sensing and biofilm development, and biofilms serve as a reservoir for releasing pathogenic planktonic cells into the environment. (ijbpr.net)
The formation of biofilms by pathogenic bacteria like Staphylococcus aureus , Haemophilus influenza , Pseudomonas aeruginosa and Leptospira interrogans may boost the pathogen's pathogenicity. (ijbpr.net)
The formation of biofilms by some pathogenic bacteria has been thoroughly investigated, but less is known about the formation of biofilms by neglected pathogens. (ijbpr.net)
Leptospira includes pathogenic, saprophytic, and intermediate species. (ijbpr.net)
L. interrogans and Leptospira biflexa were the two initial species of Leptospira , with the former housing all pathogenic strains and the latter housing saprophytes. (ijbpr.net)
The most common zoonotic illness brought on by the pathogenic Leptospira species is leptospirosis [ 3 ]. (ijbpr.net)
Several studies have illustrated the therapeutic efficacy of photoactivated riboflavin against nosocomial infections and multidrug resistant bacterial infections as well as microbial associated biofilm infections, revealing the potential role of riboflavin as a promising antimicrobial candidate, which could serve as one of the alternatives in fighting the global crisis of the emergence of antimicrobial resistance seen in different pathogenic microbes. (bioseek.eu)
As a consequence, the lower airways become persistently infected, where up to 50% of COPD patients are chronically colonized with potentially pathogenic bacteria including Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis ( 5 , 6 ). (frontiersin.org)
Fusobacterium nucleatum plays a key role in creating the pathogenic subgingival biofilm that initiates destructive periodontitis. (nature.com)

We are interested in unraveling the roles and behavior of individual bacteria in challenging environments such as the human host, and within structured multi-species communities known as biofilms. (isbscience.org)
Bacteria infecting wounds arrange themselves in polymicrobial communities known as biofilms. (ibecbarcelona.eu)
These attached communities are known as biofilms. (thepigsite.com)

The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus ( S. aureus ) growth and prevented it from forming biofilms. (frontiersin.org)
2021. TCF-ALP: a fluorescent probe for the selective detection of Staphylococcus bacteria and application in "smart" wound dressings . (cardiff.ac.uk)
This in vitro multispecies biofilm model resembles the natural conditions present in wounds and allows us to study a Pseudomonas aeruginosa and Staphylococcus aureus co-culture, which are the predominant bacteria found in wounds. (ibecbarcelona.eu)
The effects of these NPs on Acinetobacter baumannii , Staphylococcus aureus , and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, -scanning electron microscopy, and atomic force microscopy. (dovepress.com)

Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses). (lookformedical.com)
Compared to planktonic bacteria, microorganisms living inside biofilms have very different lifestyles. (ijbpr.net)

Based on the environment in which biofilm production takes place, the nature of the biofilm matrix varies. (ijbpr.net)
This gives the slimy nature of the biofilm, and contains many sugars such as fructose, glucose, mannose, rhamnose, galactose and N-acetylglucosamine. (thepigsite.com)

A significant part of the breast microbiota includes lactic acid bacteria, whose cell-free supernatant (LAB-CFS) may prevent bacteria from secreting virulence factors and lessen their pathogenicity ( 12 , 13 ). (frontiersin.org)
Taken together, transcriptomic data indicate that early responses between P. aeruginosa and S. aureus involve competition for resources and metabolic adaptations, rather than the expression of bacteria- or host-directed virulence factors. (biomedcentral.com)
Quorum Sensing (QS) a technique of communication used by bacteria in biofilms enables them to regulate biofilm formation respond to environmental stress produce secondary metabolites, and express virulence factors. (ijbpr.net)
Moreover, these tea components also attenuated F. nucleatum -mediated hemolysis and hydrogen sulfide production, two other virulence factors expressed by this bacterium. (nature.com)

contribute to the enhanced biofilm formation by L. casei, and probably S. oralis, when grown in mixed-species biofilms with S. mutans. (dna-metabolism.com)
Notably, enhanced biofilm formation by Lactobacillus plantarum and Lactobacillus rhamnosus was noted in a mucin-based medium [38], so the presence of polysaccharides may have a general ability to promote biofilm formation by lactobacilli. (dna-metabolism.com)
Selleck Omipalisib While co-cultivation with S. mutans significantly enhanced biofilm formation by L. casei, the sessile population SP600125 price of S. mutans was also found to be increased by more than 2-fold in dual species model with L. casei (Figure 2), which is contrary to what was observed with the other bacteria studied. (dna-metabolism.com)

The microbial community is also a natural source of metabolites and has the potential to be used to create antimicrobial and anti-biofilm agents. (frontiersin.org)
Scientist Philip Marsh is credited with developing the ecological plaque hypothesis in 1994, in which he ideated that dental plaque can be both normal and healthy as well as "cariogenic" (creates cavities), depending on the microbial community (or "consortia") present in the biofilm and the community's stability. (wikipedia.org)
While teeth provide stability to the microbial community, the overgrowth of bacteria is known to result in tooth decay primarily due to acid production from sugar-consuming fermentative metabolisms. (wikipedia.org)
24) Even certain anti-microbial mouth-rinses have sadly reduced our oral commensal bacteria. (probiorahealth.com)
We are developing and applying high-throughput single-cell genomic technologies to identify and characterize diverse functional subpopulations of bacteria within complex microbial samples. (isbscience.org)
A single microbial species several microbial species or a mix of prokaryotic and eukaryotic organisms may make up the community. (ijbpr.net)
The environment that biofilms offer for interacting with several other microbial species in nature is crucial to metabolism. (ijbpr.net)
i>The presence of multidrug resistant bacteria in the airways of patients with CF is associated with decreased microbial diversity and decreased pulmonary function. (biomerieux.com)
Thus, the dual effects of riboflavin on both pathogens and host immunity, reflected by its potent bactericidal effect and alleviation of inflammation in host cells further imply that riboflavin could be a potential candidate for therapeutic intervention in resolving microbial infections. (bioseek.eu)
Hence, this review aimed to provide some insights on the promising role of riboflavin as an antimicrobial candidate and also a host immune-modulator from a multi-perspective view as well as to discuss the application and challenges on using riboflavin in photodynamic therapy against various pathogens and microbial biofilm-associated infections. (bioseek.eu)
The understanding of the interaction between the components of this microbial community within the host is essential in order to develop new healing strategies that target bacteria growing in wounds. (ibecbarcelona.eu)
We propose that the anti-microbial and anti-inflammatory actions of specialized pro-resolving mediators, such as resolvin-D1 should be harnessed for the treatment of AECOPD that are complicated by the co-pathogenesis of viruses and bacteria. (frontiersin.org)

A species of MITOSPORIC FUNGI commonly found on the body surface. (lookformedical.com)
Researchers at RMIT in Australia have developed a drug-free approach to kill bacteria and fungi that can infect surfaces on medical implants. (medgadget.com)
Besides maintaining human health, different sources reported that riboflavin can inhibit or inactivate the growth of different pathogens including bacteria, viruses, fungi and parasites, highlighting the possible role of riboflavin as an antimicrobial agent. (bioseek.eu)
A biofilm is a collection of bacteria and/or fungi that exists in a multicellular or community form, encased in an extracellular polysaccharide matrix that they themselves synthesise. (thepigsite.com)

This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)
Paper II studies differences between the S. gordonii DL1 general proteomes in planktonic and biofilm growth phases, and the regulatory effects of salivary mucin MUC5B on protein expression in the biofilm cells. (diva-portal.org)
Different bacterial species exchange metabolites and metabolite by products which are not the case with planktonic bacteria. (ijbpr.net)
Bacteria within the biofilm are protected from disinfectants and have a fundamentally reduced antibacterial sensitivity compared with planktonic, free-living, bacteria. (thepigsite.com)
Free floating or planktonic bacteria encounter this monolayer and form a reversible, sometimes transient attachment to it. (thepigsite.com)

The capacity of S. aureus to build biofilms is correlated with the severity of infections ( 4 ). (frontiersin.org)
Furthermore, the ability of the pathogen to build biofilms and to persist e.g. in the lungs of cystic fibrosis (CF) patients facilitates the establishment of chronic infections, which are largely recalcitrant to antimicrobial therapies. (elifesciences.org)

31) Candida albicans, a fungal organism, helps with the biofilm production by increasing the extracellular polysaccharide matrix which protects Streptococcus mutans from anti-microbials and commensals such as Streptococcus oralis. (probiorahealth.com)

Moreover, riboflavin and flavins could produce reactive oxygen species (ROS) when exposed to light, inducing oxidative damage in cells and tissues, and thus are excellent natural photosensitizers. (bioseek.eu)
2021. A residue-free approach to water disinfection using catalytic in situ generation of reactive oxygen species . (cardiff.ac.uk)
Here, we review emerging mechanisms into why COPD patients are susceptible to chronic bacterial infections and highlight dysregulated inflammation and production of reactive oxygen species (ROS) as central causes. (frontiersin.org)
Pyocyanin not only paints the wound green but also creates reactive oxygen species, thereby damaging the host tissues and impeding their healing process. (beekeepingtrove.com)

These are believed to likely have been lactic acid bacteria, however the link between bacterial acid production and tooth decay was not further uncovered until much later. (wikipedia.org)

The inhibition of quorum sensing, called quorum quenching, may disrupt oral biofilm formation, which is the main etiological factor of oral diseases, including periodontitis. (mdpi.com)
Bacteria within a biofilm communicate by quorum sensing leading to phenotypic modifications and changes in gene expression. (thepigsite.com)

biofilms RealTime-PCR was used to analyze the expression of several genes that have critical roles in bacterial adherence and biofilm accumulation by S. mutans [7-10], including spaP, gtfB and gbpB. (dna-metabolism.com)
Formation of a biofilm starts with adherence of bacteria followed by production of the polysaccharide matrix. (thepigsite.com)

Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. (frontiersin.org)
The metabolites of S. aureus biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. (frontiersin.org)
S. aureus produces large biofilm formations that support its pathogenicity and confer protection and subsequently drug resistance ( 5 ). (frontiersin.org)
The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)
Because of this, it is crucial to understand how LAB-CFS affects the makeup and function of metabolites in S. aureus biofilms. (frontiersin.org)
AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus . (dovepress.com)

The specific responses elicited by MUC5B in paper II-III seem to promote commensal colonization while down-regulating dysbiosis-related biofilm activities. (diva-portal.org)
We show that mutualistic and abundant biofilm growth in flowing saliva of two human oral commensal bacteria, Actinomyces naeslundii T14V and Streptococcus oralis 34, is dependent upon production of AI-2 by S. oralis 34. (princeton.edu)

A Novel Fosfomycin-Trimethoprim-Sulfamethoxazole Combination Exhibits Marked In Vitro Antibacterial Synergy Against Bacteria Causative of Urinary Tract Infections. (asm.org)
Repurposing of Drugs to Fight Persistent Infections-Antibacterial Compounds Against Non-growing Bacteria. (asm.org)
Polymicrobial infections involve both detrimental and beneficial interactions between different bacterial species. (biomedcentral.com)
In this respect, detrimental and beneficial interactions between co-colonizing bacteria may influence the development and persistence of co-infections. (biomedcentral.com)
The mucus clogs the patient's airways and traps germs that lead to infections and other complications, including the growth of bacterial colonies called "biofilms" in the lungs. (biomerieux.com)
This underlying chronic infection leaves COPD patients particularly vulnerable to acute viral infections, which further destabilize host immunity to bacteria. (frontiersin.org)
Felix d'Herelle first coined the term "bacteriophage" ( 4 ), literally meaning "bacterium eater," and began using phages to treat bacterial infections in human patients ( Fig. 1 ). (asm.org)
The development of antibiotic resistance in periodontopathogens is a major issue given that these bacteria may migrate from the oral cavity to other organs where they may cause infections or transfer their antibiotic resistances to other bacterial species. (nature.com)

Scardovia wiggsiae is a Bacillus bacterium found extensively associated with Severe-Early Childhood Caries. (probiorahealth.com)

Bacteria can develop antibiotic resistance through horizontal gene transfer within biofilms. (ijbpr.net)
Also, antibiotic use can kill the nonpathogenic bacteria living in the lungs as well. (biomerieux.com)
Also, we use a set of biofilm-degrading enzymes targeting the wound biofilm so as to improve the antibiotic delivery in the local area of the infection site. (ibecbarcelona.eu)
The therapeutic use of bacteriophages, viruses that infect and kill bacteria, is well suited to be part of the multidimensional strategies to combat antibiotic resistance. (asm.org)
Given the emergence of antibiotic-resistant bacteria, new, preferably inexpensive, alternatives to conventional antibiotics must be developed. (nature.com)

We develop and apply single-cell genomic tools for unbiased, high-throughput expression profiling of bacterial consortia such as the animal-associated microbiota and single- and polymicrobial biofilms. (isbscience.org)
In addition, multiple species frequently co-occur and interact in a polymicrobial biofilm. (isbscience.org)

Antibiosis, which is defined by detrimental interactions between at least two species, usually involves the synthesis of antimicrobial molecules that inhibit growth or kill other bacterial species [ 18 ]. (biomedcentral.com)
The metabolism of different bacterial species can be influenced by the interactions between the metabolites of different organisms. (ijbpr.net)
However, classic culture methods do not allow the simultaneous co-culture of different bacterial species, or the study in a more-realistic infection site environment, where several host factors are presented. (ibecbarcelona.eu)

its role in the pathogenicity of this bacterium is alarming. (beekeepingtrove.com)

This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms. (dovepress.com)

The bacterium can cling onto wound surfaces and form biofilms, ensuring long-term survival. (beekeepingtrove.com)

Another critical role that saliva plays in the microscopic environment is supplying the glycoproteins bacteria use to cling to the surface of teeth. (wikipedia.org)
Compared to floating in saliva, bacteria on teeth gain environmental stability so that they experience a consistent environment of temperature, relative oxygen exposure, nutrient density, physical disturbances, etc. (wikipedia.org)

This thesis investigates responses to external cues in oral bacteria on a molecular level. (diva-portal.org)
Findings associated with responses to external cues in oral bacteria may contribute to future development of novel preventative strategies and identification of predictive biomarkers for oral health. (diva-portal.org)
Global responses in both bacteria included competition for nitrogen sources, amino acids and increased tRNA levels. (biomedcentral.com)
How are these responses shaped in the presence of interacting or competing species? (isbscience.org)
We aim to create single-cell gene expression maps of model single- and multiple-species biofilms and their responses to external stimuli by a combination of single-cell RNA sequencing and single-cell time-lapse imaging. (isbscience.org)

AI-2 is proposed to be a universal signal molecule mediating interspecies communication among bacteria. (princeton.edu)

Paper III examines the regulatory roles of salivary MUC5B on biofilm attachment and metabolic output in two clinical isolates of oral commensals, S. gordonii CW and Actinomyces naeslundii CW. (diva-portal.org)

However the development of biofilms on surgical implants, prosthetic valves, feeding tubes and catheters has a significant impact on human health. (ijbpr.net)

Unlike wild-type dual-species biofilms, A. naeslundii T14V and an S. oralis 34 luxS mutant did not exhibit mutualism and generated only sparse biofilms which contained a 10-fold lower biomass of each species. (princeton.edu)

We use a novel in vitro culture approach, optimizing a method to assess bacterial viability in a wound biofilm model. (ibecbarcelona.eu)
In addition, the treatment of a pre-formed F. nucleatum biofilm with the green tea extract and EGCG caused a time-dependent decrease in biofilm viability. (nature.com)

A unique three-dimensional self-produced matrix that provides greater food supplies and supports enhanced growth and survival against biocides holds this community of bacteria together. (ijbpr.net)
Through these interactions bacteria in the biofilm acquire biocide tolerance and resistance enabling them to endure in the presence of biocides. (ijbpr.net)

As compared to mono-species Y-27632 2HCl biofilms, expression of luxS was decreased by more than 7-fold in cells grown with L. casei (P (dna-metabolism.com)

The features displayed by the biofilm hinder and delay the healing processes, as such bacterial communities exhibit higher resistance to antibiotics and higher ability to evade the immune response. (ibecbarcelona.eu)

Microbiota, complex consortia of bacteria, are found in all environments, including the human body. (isbscience.org)

As replication is a crucial step to initiate an infection, we have first focused on the study of the differential role of the different P. aeruginosa Ribonucleotide Reductase (RNR) enzymes in bacterial growth within the wound biofilm model, as RNR are essential enzymes in DNA replication. (ibecbarcelona.eu)

Both bacteria were also able to utilize MUC5B as a sole nutrient source during early biofilm formation, individually and synergistically in a dual species biofilm. (diva-portal.org)
While the close association of L. casei and S. mutans in carious sites is well documented, little information is available concerning the interaction between these two bacteria with respect to S. mutans biofilm formation and its cariogenicity. (dna-metabolism.com)
This typically leads to a nasty infection, which is often complicated by biofilm formation, and may require the eventual removal of the implant. (medgadget.com)
Control of biofilms is difficult and addresses preventing initial formation and removing existing biofilms. (thepigsite.com)
The formation of a biofilm is not a random process. (thepigsite.com)
They also prevented biofilm formation by F. nucleatum at concentrations that did not interfere with bacterial growth. (nature.com)
It plays a key role in subgingival biofilm formation by bridging the early colonizers (streptococci and actinomyces) and the late colonizers ( Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola ) 3 that make up the red complex initially described by Socransky et al . (nature.com)
Given its central role in biofilm formation, F. nucleatum may represent a key target for controlling biofilm formation. (nature.com)

The cellular outcome of the infection may range from complete clearance of infection to persistent survival of intracellular bacteria. (isbscience.org)

Thus, AI-2 acts as an interspecies signal and its concentration is critical for mutualism between two species of oral bacteria grown under conditions that are representative of the human oral cavity. (princeton.edu)

slight decreases were observed in expression of spaP, gtfB and gbpB by S. mutans when grown in dual-species with S. sanguinis as compared to those in mono-species biofilms, although the differences were not statistically significant. (dna-metabolism.com)
When grown in dual-species with L. casei, however, expression of spaP, gbpB and gtfB by S. mutans was decreased by as much as 40-fold, at a significance level of P (dna-metabolism.com)
Single-cell studies reveal heterogeneity of bacterial gene expression even in genetically identical bacteria grown under the same lab conditions. (isbscience.org)
Given the complexity observed even in lab-grown monocultures, this heterogeneity is likely further magnified within complex environments and in the presence of other species, where bacteria employ bet-hedging and labor division strategies to enhance their fitness. (isbscience.org)

The bacterium is famed for its unique biofilm - a protective shield composed of extracellular polymers. (beekeepingtrove.com)

A recent study of Hadza infants demonstrated that over 23% of their gut microbiome contained bacteria that were "novel" to western nation infants. (probiorahealth.com)

Proteins found in any species of fungus. (lookformedical.com)

Bacteria are typically found as communities made up of several different species rather than as isolated, solitary organisms in nature ( 6 ). (frontiersin.org)
Other bacteria, other micro-organisms and debris become incorporated in the biofilm. (thepigsite.com)

Biofilms are best removed by oxidative disinfectants (Virkon® S or Hyperox® from DuPont Animal Health Solutions). (thepigsite.com)

As a shapeshifter in the world of bacteria, the Pseudomonas encapsulates an enigma that both baffles and fascinates scientists, medical professionals, and patients alike. (beekeepingtrove.com)
Pseudomonas aeruginosa , famed in medical circles as a tenacious bacterium, is known for its resilience and adaptability. (beekeepingtrove.com)

The interactions between cells and their surroundings are primarily responsible for the creation of biofilms. (ijbpr.net)

Bacteria settle on the tooth surface as a solid substrate on which they grow. (wikipedia.org)
If the attachment of the bacteria to the substrate occurs for long enough (probably a few minutes) other physical and chemical structures are produced which transform the attachment to an irreversible one. (thepigsite.com)

Anatomy1

Organisms18

Diseases1

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes17

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science2

Health Care4

Pathogenic17

Known as biofilms3

Staphylococcus4

Microorganisms2

Nature of the biofilm2

Virulence4

Enhanced biofilm formation3

Microbial12

Fungi4

Planktonic6

Build biofilms2

Candida1

Reactive oxygen4

Lactic acid bac1

Quorum2

Adherence2

Aureus6

Commensal2

Infections8

Bacillus1

Antibiotic5

Polymicrobial2

Bacterial species3

Pathogenicity1

Inhibition1

Surfaces1

Saliva2

Responses5

Among bacteria1

Metabolic1

Catheters1

Naeslundii1

Viability2

Biocides2

LuxS1

Resistance1

Consortia1

Wound1

Formation8

Survival1

Oral cavity1

Grown4

Extracellular polymers1

Microbiome1

Proteins1

Organisms2

Oxidative1

Pseudomonas2

Interactions1

Substrate2