AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsInformation Science
DrosophilaDrosophila ProteinsDrosophila melanogasterRNANucleic Acid HybridizationIn Situ HybridizationMolecular Sequence DataRNA ProbesBase SequenceRNA, MessengerAmino Acid SequenceImmunohistochemistryGene ExpressionFluorescent Antibody TechniquePlant ProteinsAnimals, Genetically ModifiedInsect ProteinsLarvaMutationGene Expression Regulation, DevelopmentalEmbryo, NonmammalianWingNuclear Localization SignalsTranscription FactorsPhotoreceptor Cells, InvertebrateInsect HormonesSequence Homology, Amino AcidCell NucleusPhenotypePupaCloning, MolecularEyeGenome, InsectOogenesisNuclear ProteinsTranscription, GeneticDNA-Binding ProteinsIn Situ Hybridization, FluorescenceSignal TransductionRNA InterferenceChromosome MappingBody PatterningMembrane ProteinsCytoplasmProtein TransportMorphogenesisCell LineGenes, LethalRecombinant Fusion ProteinsSequence AlignmentCrosses, GeneticGreen Fluorescent ProteinsProtein BindingHomeodomain ProteinsProtein Structure, TertiaryChromosomesWnt1 ProteinConserved SequenceDNA, ComplementaryDNAMicroscopy, FluorescenceSpecies SpecificityEvolution, MolecularSubcellular FractionsModels, BiologicalRepressor ProteinsCell PolarityX ChromosomeNervous SystemBlastodermDNA Transposable ElementsEcdysoneSense OrgansDNA PrimersMushroom BodiesNerve Tissue ProteinsRNA, UntranslatedAllelesReceptors, NotchBinding SitesModels, GeneticGenes, HomeoboxMetamorphosis, BiologicalPhylogenyMicroscopy, ConfocalChromosomes, InsectTransgenesCarcinoma in SituTissue DistributionFat BodyNeuronsCarrier ProteinsNucleic Acid ConformationSequence Homology, Nucleic AcidHeLa CellsRNA, Small InterferingBlotting, NorthernLuminescent ProteinsCentral Nervous SystemActive Transport, Cell Nucleus
HybridizationMelanogasterProteinsGenesMammalianMRNAsTissuesHighly conservedTranscriptional regulatorProteinEmbryoMRNA localizationSubcellular localizationGenomeNeuronalNeuronsEmbryonicGermDepletionRibosomalApicalEncodesGastrulationLarvalRegulationMolecularProbes2017CharacterizationRepeatsMitochondrialRegulatorMotifsIsolationEndogenousIntracellularRetinaGene expression patternsMembraneMutantsSynapticPatternsBiologicalMicroarrayPathwayComplementaryDevelopmentCellRegulatoryMoleculesLargely
Hybridization11
Melanogaster10
Proteins21
  • The Drosophila Extramacrochaetae protein antagonizes the proneural function of the Achaete and Scute proteins in the generation of the adult fly sensory organs. (biologists.com)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. (nature.com)
  • The Nanos proteins family regroups highly conserved RNA-binding proteins in higher eukaryotes implicated in germ cell development and maintenance. (nature.com)
  • These genes, encoding for RNA binding proteins, contain a highly conserved RNA recognition motif and at least one DAZ repeat encoding for a 24 amino acids sequence able to bind other mRNA binding proteins. (ijbs.com)
  • This family includes three members, Boule (or Boll ), Dazl (or Dazla ) and DAZ , encoding RNA binding proteins. (ijbs.com)
  • The localization of DAZ family proteins suggests that they can regulate mRNA translation occurring in the cytoplasm. (ijbs.com)
  • These proteins have a highly conserved RNA recognition motif (RRM) for binding target mRNAs and at least one characteristic sequence of 24 amino acids, which are termed as DAZ repeats [ 1 ]. (ijbs.com)
  • Dazl can regulate the expression, transport and localization of target mRNAs of proteins which control the differentiation, growth and maturation of germ cells. (ijbs.com)
  • Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
  • Although Rab11a subcellular localization has been demonstrated by transgenic zebrafish [10], and molecular function of Rab11 proteins in primary cilia membrane assembly in Kupffer Vesicle has been studied [6], the developmental expression profile of Rab11 proteins in zebrafish remains elusive. (researchsquare.com)
  • The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. (nih.gov)
  • The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
  • Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase. (stanford.edu)
  • Long-term memory consolidation: The role of RNA-binding proteins with prion-like domains. (ncbs.res.in)
  • Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins. (colorado.edu)
  • At the sub-cellular scale, maps document the spatial organization of proteins, RNA, DNA, and metabolites with nanometer precision and temporal acuity on the order of seconds. (stanford.edu)
  • Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice. (shengsci.com)
  • Finally, we showed that Bonus SUMOylation is mediated by the SUMO E3-ligase Su(var)2-10, revealing that although SUMOylation of TIF1 proteins is conserved between insects and mammals, both the mechanism and specific site of modification is different in the two taxa. (elifesciences.org)
  • In vertebrates this subfamily contains four proteins: TIF1α/TRIM24, TIF1β/TRIM28, TIF1γ/TRIM33, and TIF1δ/TRIM66, while only one protein, Bonus (Bon), is present in Drosophila , making it an attractive model to understand the conserved functions of TIF1 proteins. (elifesciences.org)
  • This mitochondrial RNA colocalizes with components of the cytoplasmic machinery that makes and imports nuclear-encoded proteins - that is, a ribosomal protein (S6), a nascent peptide associated protein (NAC), and the translocase in the outer membrane (Tom22). (biomedcentral.com)
Genes13
  • His work there focused on the molecular characterization of segmentation genes in Drosophila. (wikipedia.org)
  • Swr1 is required for the deposition of histone H2AZ at specific chromosome locations in vivo, and Swr1 and H2AZ commonly regulate a subset of yeast genes. (sdbonline.org)
  • In Vertebrates, Nanos paralogous genes were associated to partial redundancies and specific functional evolutions. (nature.com)
  • In teleostean fish, two nanos1 genes were generated, probably during the Teleost specific whole genome duplication (3R). (nature.com)
  • Loss of PPan, but not interference with rRNA processing, led to an early downregulation of specific marker genes of the eye, including Rx1 and Pax6 , and of NCCs, such as Twist, Slug and FoxD3 . (silverchair.com)
  • In Drosophila males, meiotic prophase lasts 3.5 days, during which spermatocytes upregulate of over 1800 genes and grow 25-fold. (stanford.edu)
  • We identified >500 Drosophila genes that express mRNA isoforms with a long 3' UTR in proliferating spermatogonia but a short 3' UTR in differentiating spermatocytes due to APA. (stanford.edu)
  • Embryos fluorescently stained for both RNA (red) and protein (cyan) products of the gap genes Krüppel are shown at early (A), mid (B) and late (C) cleavage cycle 14A during the blastoderm stage of Drosophila development. (drosophila-images.org)
  • The characterization of new miRNA target genes and a unique profile for crasiRNAs has allowed for insight into multiple RNA mediated processes in the tammar, including gene regulation, species incompatibilities, centromere and chromosome function. (biomedcentral.com)
  • This method was used to measure the number of copies of repeated genes such as rRNA genes (2) and to measure whether specific genes were under-replicated during the replication process used in forming polytene chromosomes (3). (ddw-online.com)
  • All these studies have shown that spalt is activated in response to several signal transduction pathways in different tissues and developmental processes [ 11 ], and the expression patterns and functions of sall genes, together with the analysis of their regulation, indicate they cannot be universally assigned to a specific signaling pathway. (biomedcentral.com)
  • Third, even using currently available comprehensive arrays, tissue-specific genes are still under-represented, including alternatively spliced variants or genes that are only briefly expressed in a tissue-specific pattern. (biomedcentral.com)
  • Interestingly, RNA-seq of arlr mutant adipose tissues and RIP-seq suggest that Arlr affects lipid metabolism through the degradation of the mRNAs of lipolysis genes - a model further supported by the observation that knockdown of Lsd-1, regucalcin, yip2 or CG5162, which encode genes involved in lipolysis, rescue the LD defects of arlr mutants. (bvsalud.org)
Mammalian7
  • These participate in transcriptional/translational feedback loops and many homologous clock-components in the fruit fly Drosophila are also expressed in mammalian clock tissues with circadian rhythms. (bioone.org)
  • This study shows that Schip1 , a Drosophila homolog of the mammalian Schwannomin interacting protein 1 (SCHIP1), provides a link between Ex and Hpo. (sdbonline.org)
  • The molecular link between splicing and mRNA localization/local translation has also been demonstrated in mammalian neurons. (frontiersin.org)
  • Further study on the differences between mammalian RyR-FKBP12 and Drosophila DmRyR-FKBP12 can be exploited to develop safe pesticides. (biomedcentral.com)
  • As part of the genome sequencing initiative for the tammar, we have explored the evolution of each of the major classes of mammalian small RNAs in an Australian marsupial for the first time, including the first genome-scale analysis of the newest class of small RNAs, centromere repeat associated short interacting RNAs (crasiRNAs). (biomedcentral.com)
  • This important study advances our understanding of the functions and regulation of the Drosophila transcriptional regulator Bonus, an ortholog of mammalian TIF1 family members. (elifesciences.org)
  • Here, we characterize the Drosophila endoribonuclease CG2145, an ortholog of mammalian EndoU that we named Age-related lipid regulator (Arlr), as a regulator of lipid homeostasis during aging. (bvsalud.org)
MRNAs2
  • They can bind to the 3′ untranslated region (3′UTR) of target messenger RNAs (mRNAs) in order to regulate gene expression post-transcriptionally with no sequence specificity but by potential electrostatic interactions with the phosphate backbone of RNA 2 , 3 . (nature.com)
  • The first class of small RNAs identified were the micro RNAs (miRNAs), which are small (~22 nt) non-coding RNAs that regulate gene expression by base pairing to mRNAs where they direct either mRNA cleavage or repress translation [ 8 ]. (biomedcentral.com)
Tissues2
  • Extracellular vesicle DNA from human melanoma tissues contains cancer-specific mutations. (gu.se)
  • First, temporally and spatially restricted tissue or cell patterns cause difficulties in dissecting target tissues or cells to collect enough RNA. (biomedcentral.com)
Highly conserved1
  • We examined each of these small RNA classes with respect to the newly assembled tammar wallaby genome for gene and repeat features, salient features that define their canonical sequences, and the constitution of both highly conserved and species-specific members. (biomedcentral.com)
Transcriptional regulator1
  • In this study, we identified both temporal and localization changes in expression of DmFKBP12, a translational and transcriptional regulator of Drosophila RyR (DmRyR) and FKBP12, through embryonic development. (biomedcentral.com)
Protein14
  • A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. (nih.gov)
  • Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. (nih.gov)
  • Previous work showed that the cell cycle regulator Cyclin B (CycB) is subject to translational repression in immature spermatocytes, mediated by the RNA-binding protein Rbp4 and its partner Fest. (stanford.edu)
  • Here we show that the spermatocyte-specific protein Lut is required for translational repression of cycB in an 8-hour window just before spermatocytes are fully mature. (stanford.edu)
  • In addition, spermatocyte-specific isoforms of Syncrip (Syp) are required for expression of CycB protein in mature spermatocytes and normal entry into the meiotic divisions. (stanford.edu)
  • Thus a set of spermatocyte-specific regulators choreograph the timing of expression of CycB protein during male meiotic prophase. (stanford.edu)
  • APA can potentially affect mRNA translation efficiency, localization, stability, and mRNA seeded protein-protein interactions. (stanford.edu)
  • IP3R mediated Ca(2+) release regulates protein metabolism in Drosophila neuroendocrine cells: implications for development under nutrient stress. (ncbs.res.in)
  • We used the amyotrophic lateral sclerosis-associated protein TDP-43 as a primary model to investigate the effects of clusterin on protein aggregation and neurotoxicity in complementary in vitro, neuronal cell and Drosophila systems. (biomedcentral.com)
  • Dynamic shifts of the posterior boundary of the gap gene Krüppel as visualized by asymmetric distribution of RNA and protein expression domains. (drosophila-images.org)
  • In (B) and (C), the RNA domain is located more anteriorly than the protein domain, indicating that Krüppel is only expressed in the anterior portion of the protein domain. (drosophila-images.org)
  • Although DmFKBP12 is distributed evenly between the anterior to posterior poles of the blastoderm egg, the protein is expressed more strongly in the cortex of the early Drosophila gastrula with the highest concentration found in the basement membrane of the cellular blastoderm. (biomedcentral.com)
  • Protein-coding regions of the resulting RNAs are translated by ribosomes within the mitochondrion. (biomedcentral.com)
  • Polyglutamine-binding protein 1 ( PQBP1 ) is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by severe cognitive impairment, but also by physical defects that suggest PQBP1 has broader functions in embryonic development. (xenbase.org)
Embryo3
  • Dynamic control of positional information in the early Drosophila embryo. (drosophila-images.org)
  • Simultaneous visualization of nascent transcripts from two enhancers and a promoter of the Hox gene Abdominal-B in a Drosophila embryo. (drosophila-images.org)
  • In the Drosophila embryo, apical constriction in a strip of epithelial cells along the ventral midline results in the folding of the tissue and the internalization of ventral cells, forming a ventral furrow (VF). (nature.com)
MRNA localization1
  • Consistent with this finding, core components of EJC (eIF4AIII, Barentsz, Tsunagi, and Mago nashi) are critical for oskar mRNA localization ( Hachet and Ephrussi, 2001 , 2004 ). (frontiersin.org)
Subcellular localization1
  • however, the subcellular localization of the channels in HCs has not been fully resolved. (jneurosci.org)
Genome6
  • Based on the work on segmentation processes in Drosophila, he developed a general interest in studying the evolution of segmentation processes, especially in the model system Tribolium and he was involved in generating the first full genome sequencing for this beetle species. (wikipedia.org)
  • These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome defense. (biomedcentral.com)
  • This study encompasses the first analyses of the major classes of small RNAs for the newly completed tammar genome, validates preliminary annotations using deep sequencing and computational approaches, and provides a foundation for future work on tammar-specific as well as conserved, but previously unknown small RNA progenitors and targets identified herein. (biomedcentral.com)
  • Small RNAs play important roles in many aspects of pre- and post-transcriptional gene regulation, epigenetic modifications, chromosome segregation and genome structure. (biomedcentral.com)
  • The kinetics of replication and transcription (monitored by immunolabelling after incorporating bromodeoxyuridine or bromouridine) reveal that each genome replicates independently of others in a focus, and that newly-made RNA remains in a focus (residence half-time ~43 min) long after it has been made. (biomedcentral.com)
  • In most human cells, mitochondria contain 10 3 -10 4 copies of a circular genome of 16,569 base-pairs that encodes two ribosomal RNAs, 22 tRNAs, and 13 polypeptides that form parts of the respiratory-chain located in the inner mitochondrial membrane [ 9 , 10 ]. (biomedcentral.com)
Neuronal1
  • Fertilized egg, through the profile as under-membrane cortex distribution concentering onto basement at cellular blastoderm, to the profile as three-gem layer localization in primitive neuronal and digestion architecture of early Drosophila gastrula. (biomedcentral.com)
Neurons5
  • Using functional imaging and behavioral analysis, loss of Tao-induced ectopic synapses with inappropriate partner neurons were shown to be functional and altered behavioral responses in a connection-specific manner. (sdbonline.org)
  • RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)
  • We further showed that the expression of TDP-43 in transgenic Drosophila neurons induced ER stress and that co-expression of clusterin resulted in a dramatic clearance of mislocalized TDP-43 from motor neuron axons, partially rescued locomotor activity and significantly extended lifespan. (biomedcentral.com)
  • We used the Drosophila midgut2 to investigate this and discovered that during regeneration a subpopulation of cholinergic3 neurons triggers Ca2+ currents among intestinal epithelial cells, the enterocytes, to promote return to homeostasis. (bvsalud.org)
  • We found that downregulation of the conserved cholinergic enzyme acetylcholinesterase4 in the gut epithelium enables acetylcholine from specific Egr5 (TNF in mammals)-sensing cholinergic neurons to activate nicotinic receptors in innervated enterocytes. (bvsalud.org)
Embryonic3
  • Cell and tissue specific gene expression is a defining feature of embryonic development in multi-cellular organisms. (biomedcentral.com)
  • the process that governs the early embryonic patterning of the Drosophila body plan is now the best understood example of a complex cascade of transcriptional regulation during development [ 14 , 15 ]. (biomedcentral.com)
  • Eleven stages of mouse retina, from embryonic day 12.5 (El2.5) to postnatal day 21 (PN21), were collected for RNA isolation. (biomedcentral.com)
Germ3
  • During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2 period termed meiotic prophase. (stanford.edu)
  • In Drosophila, the germ granules, or from proline to leucine. (lancasterarchitecture.com)
  • Kohn M, Hameister H, Vogel M, Kehrer-Sawatzki H. Expression pattern of localization closely mirrors that of DCP1 does not colocalize with DCP1 and degradation factors localize to germ granules when the bacteria are exposed to bacteria expressing a crRNA guiding Cas12a (no crRNA). (lancasterarchitecture.com)
Depletion1
  • STED, stimulated emission depletion IntroductionRibonucleoprotein (RNP) granules are biomolecular condensates containing RNAs and become activated. (lancasterarchitecture.com)
Ribosomal1
  • A dominant negative mutant of the E. coli RNA helicase DbpA blocks assembly of the 50S ribosomal subunit. (colorado.edu)
Apical1
Encodes1
Gastrulation1
  • During Drosophila gastrulation, actomyosin contraction in ventral cells generates a long, narrow epithelial furrow, termed the ventral furrow, in which actomyosin fibres and tension are directed along the length of the furrow. (nature.com)
Larval2
  • At the Drosophila larval neuromuscular junction (NMJ) , the retrograde BMP pathway is part of the machinery that controls NMJ expansion concurrent with larval growth. (sdbonline.org)
  • To gain insight this study quantitatively investigated synaptic growth and connectivity in the Drosophila nociceptive network during larval development. (sdbonline.org)
Regulation1
  • Throughout these changes in distribution, in situ DmFKBP12 mRNA monitoring detected equal distribution of DmFKBP12 mRNA, once again indicating that regulation of DmFKBP12 occurs at the translational level in Drosophila development. (biomedcentral.com)
Molecular3
  • Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
  • The study of PSCs has relied on an anatomy-based approach, as the identities of cell-specific PSC molecular markers have remained elusive. (elifesciences.org)
  • This has been due to the absence of a cell-specific molecular marker with which PSCs can be identified, isolated, and genetically manipulated. (elifesciences.org)
Probes1
  • Confocal imaging was used on embryos hybridized with RNA FISH probes for the IAB5 enhancer (green), the IAB8 enhancer (blue), and the Abdominal-B promoter region (red). (drosophila-images.org)
20171
  • 2017. In situ modeling of multimodal floral cues attracting wild pollinators across environments. . (ncbs.res.in)
Characterization1
Repeats1
  • TDRD1/MTR-1, which contains Tudor domain repeats, is a specific component of. (shengsci.com)
Mitochondrial1
  • We observe remarkably heterogeneous spatial and temporal expression patterns of smORF transcripts-indicating wide-spread tissue-specific and stage-specific mitochondrial architectures. (bvsalud.org)
Regulator2
  • As a critical regulator of the DmRyR-FKBP complex, DmFKBP12 expression in Drosophila fluctuates temporally and geographically with the formation of organ systems. (biomedcentral.com)
  • Together, our work identified Bonus as a regulator of tissue-specific gene expression and revealed the importance of SUMOylation as a regulator of complex formation in the context of transcriptional repression. (elifesciences.org)
Motifs2
  • Nanos family is characterized by two specific Cys-Cys-His-Cys zinc finger motifs at the carboxy-terminal region which present a regular spacing between the Cys and the His residues. (nature.com)
  • The Rab11 family is composed of three members of GTPases, Rab11a, Rab11b and Rab25, based on specific motifs [8]. (researchsquare.com)
Isolation1
Endogenous1
  • Diverse KRAB-ZFPs recognize specific DNA sequences with the majority targeting endogenous retroviruses, ensuring their repression. (elifesciences.org)
Intracellular1
  • We therefore conclude that increased expression of clusterin can provide an important defense against intracellular proteotoxicity under conditions that mimic specific features of neurodegenerative disease. (biomedcentral.com)
Retina3
  • Phylogenetic analysis demonstrated that this rhodopsin belongs to the retina-type but not to the pineal-specific rhodopsin group. (bioone.org)
  • Bubble-like assembly of cells in the developing Drosophila retina. (drosophila-images.org)
  • A tissue-specific cDNA microarray was generated using a set of sequence non-redundant EST clones collected from mouse retina. (biomedcentral.com)
Gene expression patterns1
Membrane1
Mutants1
Synaptic1
  • Therefore, the discovery of markers specific to PSCs is necessary to advance our understanding of PSCs, and synaptic glia in general, on multiple fronts. (elifesciences.org)
Patterns1
Biological2
  • Furthermore, disrupting the selective targeting of how to buy cheap pentasa online the total nos RNA level in different biological replicates are plotted with error bars indicating standard deviation. (lancasterarchitecture.com)
  • Each class of small RNAs is synthesized by a distinct mechanism and each has discrete biological functions. (biomedcentral.com)
Microarray1
  • Non-amplified RNAs were labeled for microarray experiments and three sets of data were analyzed for significance, hierarchical relationships, and functional clustering. (biomedcentral.com)
Pathway2
  • In Drosophila, Hpo and Warts kinases are core components of this pathway and function as tumor suppressors by inhibiting Yorkie (Yki) . (sdbonline.org)
  • In the Drosophila thorax, opposing gradients of Dachsous and Four-jointed expression, which are constituents of the Fat/Dachsous/Four-jointed planar cell polarity pathway, result in the polarized localization of the myosin Dachs 9 . (nature.com)
Complementary1
  • Gillespie and Spiegelman (1) observed that single stranded DNA binds strongly to nitrocellulose membranes in a manner that minimises the two strands reassociating with each other, but allows the hybridisation to complementary RNA. (ddw-online.com)
Development2
  • However, localization and characteristics of the photoreceptor that mediates photoperiodism in gonadal development remain to be determined. (bioone.org)
  • In addition to temporal changes in expression, dynamic expression profiles during Drosophila development also revealed DmFKBP12 localization. (biomedcentral.com)
Cell1
  • This limited approach has precluded our ability to isolate and genetically manipulate PSCs in a cell specific manner. (elifesciences.org)
Regulatory1
  • Small RNAs have proven to be essential regulatory molecules encoded within eukaryotic genomes. (biomedcentral.com)
Molecules1
Largely1