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  • eukaryotic
  • Our data demonstrate that in contrast to the E. coli ribosome, which preferentially recognizes the Shine-Dalgarno sequence, eukaryotic ribosomes (such as those found in retic lysate used in in vitro translation systems) can efficiently use either the Shine-Dalgarno or the Kozak ribosomal binding sites. (thermofisher.com)
  • ribosome
  • To achieve these high levels of ribosome production, eukaryotes have multiple copies of ribosomal (r)DNA, arranged in tandem in the Nucleolus Organizer Regions (NORs) of one or more chromosomes. (biomedcentral.com)
  • We find that inhibition of muscle ribosome synthesis leads to reduced systemic insulin-like growth factor signaling via two endocrine responses-decreased expression of brain derived Drosophila insulin-like peptides (dILPs) and increased expression of Imp-L2, an inhibitor of insulin signaling. (prolekare.cz)
  • human
  • In support of this were experiments executed in vivo that showed that human S3 and the Drosophila site-directed glutamine-changed S3 performed poorly when compared with Drosophilawild-type S3 and its ability to protect a bacterial mutant from the harmful effects of DNA-damaging agents. (iupui.edu)
  • ribosomes
  • Alternatively, a quantitative model predicts that a reduction in the number of ribosomes may specifically affect the translation of inefficient mRNAs through increased ribosomal competition ( 6 ). (asm.org)
  • molecular
  • However, despite the advances in nucleolar research, the sequence of molecular events that coordinates ribosomal biogenesis with cell growth, especially in highly proliferative cells, such as cancer cells, is poorly understood. (prolekare.cz)
  • sequence
  • We suggest that ribosomal RNA data should be more closely examined with regard to these questions, and we emphasize the importance of developing multiple sequence data sets. (docplayer.net)
  • bind
  • Our RiboMinus™ technology utilizes specific locked nucleic acid (LNA®) capture probes to bind ribosomal RNA and subsequently remove it from the sample via binding to streptavidin-coated Dynabeads® magnetic beads. (thermofisher.com)
  • closely
  • Ribosomal RNA trees show the myxogastrid and dictyostelid slime molds as unrelated early branching lineages, but actin and -tubulin trees place them together as a single coherent (monophyletic) group, closely related to the animal fungal clade. (docplayer.net)
  • sites
  • Unexpectedly, the δ-elimination reaction at AP sites was also abrogated by a change in the Drosophila glutamine residue. (iupui.edu)
  • activity
  • Drosophila S3 also possesses an apurinic/apyrimidinic (AP) lyase activity in which the enzyme catalyzes a β-elimination reaction that cleaves phosphodiester bonds 3′ and adjacent to an AP lesion in DNA. (iupui.edu)
  • Here we show that the replacement of the Drosophila glutamine by an alanine residue leads to the complete loss of glycosylase activity. (iupui.edu)
  • Based on these findings, we propose a model that explains how PARP1 activity impacts nucleolar functions and, consequently, ribosomal biogenesis. (prolekare.cz)
  • large
  • THE ribosomal (r)DNA of metazoan animals is a large multigene family consisting of one or more arrays of tandemly repeated units. (genetics.org)