Here, we investigated, in females of both D. melanogaster and D. nigrosparsa , the gene expression between animals uninfected and infected with w Mel, using RNA sequencing to see if the two Drosophila species respond to the infection in the same or different ways. (nature.com)
Drosophila melanogaster and D. simulans rescue strains produce fit offspring, despite divergent centromere-specific histone alleles. (shengsci.com)
Further support to this similarity has been provided by the ability of the env-like gene of DmeGypV (the Gypsy endogenous retrovirus of Drosophila melanogaster) to promote infection of Drosophila cells by a pseudotyped vertebrate retrovirus vector. (lookformedical.com)
After acute injury in muscle of both mouse and the fruit fly Drosophila melanogaster (used as alternative in vivo model) this study found that RACK1 accumulated in regenerating fibers while it declined with the progression of repair process. (sdbonline.org)
Within each tissue, downstream PCP effectors link the signal to tissue specific readouts such as stereocilia orientation in the inner ear and hair follicle orientation in vertebrates or the polarization of ommatidia and wing hairs in Drosophila melanogaster. (uni-wuerzburg.de)
In this study, novel probiotic and synbiotic formulations are shown to combinatorially extend longevity in male Drosophila melanogaster through mechanisms of gut-brain-axis communication with implications in chronic disease management. (provivapharma.com)
Instar larvae4
In Drosophila expression of miR-10-3p is highest in 12- to 24-hour-old embryos and in 1st and 3rd instar larvae. (wikipedia.org)
The gene codes for a 650 nt transcript which is expressed in greater abundance in ovaries, embryos, and third instar larvae relative to other tissues and developmental stages. (marquette.edu)
In particular, early third instar larvae are largely unresponsive to challenge with DAP-type PGN-containing microbes, while wandering larvae, which have received a large bolus of 20E as part of their developmental program, are highly responsive to these infections [ 9 ]. (biomedcentral.com)
Levels of miR-10-5p are highest in 12- to 24-hour-old embryos and much lower in larvae. (wikipedia.org)
In stage 5 Drosophila embryos (130-180 minutes post-fertilisation), miR-10 is distributed throughout 50-80% of the length of the egg. (wikipedia.org)
Expressionanalysis indicated that Bmuccr was highly expressed in larva stage and was down-regulated in embryos stage and adultstage of silkworm. (researchgate.net)
Protein5
The ribosomal protein Rp49 was used as a loading control. (biomedcentral.com)
Computer analysis of the DNA sequence of the 21C cDNA revealed a 76 percent 3$\sp\prime$ coding sequence similarity with the Drosophila acidic ribosomal protein gene, A1 (Qian et al. (marquette.edu)
One clone hybridized to the Minute locus 5E, a region containing the gene for acidic ribosomal protein 7/8 (Burns et al. (marquette.edu)
Among the downregulated genes, we discovered that FOXO was required for suppression of many ribosomal protein genes and genes involved in oxidative phosphorylation, pointing to a role for FOXO in limiting energetically costly processes such as protein synthesis and mitochondrial activity upon hypoxic stress. (thegallolab.com)
Intramuscular S1P administration results in increased degrees of complete and phosphorylated S1PR1 and ribosomal protein S6. (hsp70inhibitor.com)
Proteins1
1984). These results suggest that the 3$\sp\prime$ sequences common to the genomic clones define a subset of Drosophila ribosomal proteins that share similar functional residues. (marquette.edu)
Genes3
There are many potential targets for miR-10* in Drosophila, including several Hox genes, indicating that miR-10* may also be functional. (wikipedia.org)
The aim of this study was to identify and compare regions of nucleotide sequence homology between similar genes that are abundantly expressed and maternally transmitted during Drosophilaoogenesis. (marquette.edu)
To identify such genes, a Drosophila egg chamber cDNA library was differentially screened such that maternal RNA sequences were readily identified and selected from the recombinant population. (marquette.edu)
Gene expression1
He was one of the first researchers to discover ribosomal frameshifting as a gene expression mechanism in plant RNA viruses and to demonstrate recombination between transgene mRNA and genomic RNA of an infecting virus. (bio5.org)
Midgut1
The Y gene, which controls uptake of carotenoids from Aurora B inhibitor the midgut epithelium and larvae of mutants with the Y phenotype can't absorb dietary carotenoids. (hsp70inhibitor.com)
Insect1
We utilized Drosophila as an insect model to generate transgenic strains expressing Caenorhabditis elegans PUFA biosynthetic enzymes and examined their effects on the proportion of fatty acids. (bvsalud.org)
For instance, the gut microbiome can mediate genotype effects on the phenotype: In Drosophila , the host genotype influences the microbiome composition, leading to differences in nutrition between phenotypes [ 11 ]. (biomedcentral.com)
Wolbachia1
The Wolbachia strain w Mel was recently found to increase locomotor activities and possibly trigger cytoplasmic incompatibility in the transinfected fly Drosophila nigrosparsa . (nature.com)
Nuclear3
Genetic variation refers to the variation in the genetic material of a population, and includes the nuclear, mitochondrial, ribosomal DNA as well as the DNA of other organelles. (ndltd.org)
Dorsoventral (DV) patterning of the Drosophila embryo is initiated by a broad Dorsal (Dl) nuclear gradient, which is regulated by a conserved signaling pathway that includes the Toll receptor and Pelle kinase. (sdbonline.org)
However, in order to strengthen the signal given by COI , we added a nuclear gene to the study, namely the ribosomal internal transcribed spacer 2 ( ITS2 ). (arphahub.com)
A,B ) Developmental timing of germ-free (GF) larvae (grey) and Lp-associated larvae (green) on FLY AA diets with a -70% ( A ) or -60% ( B ) decrease in each essential AA (EAA). (elifesciences.org)
C ) Developmental timing of larvae raised on balanced diet (FLY AA, filled circles) or imbalanced diet (FLY AA -60% Val, empty circles). (elifesciences.org)
D ) Developmental timing of larvae raised on balanced diet (FLY AA, filled circles), on imbalanced diet (FLY AA Val -60%, empty circles), on imbalanced diet adjusted with His (FLY AA Val -60%+His, triangles) or on imbalanced diet adjusted with Leu (FLY AA Val -60%+Leu, squares). (elifesciences.org)
A ) Developmental timing of larvae raised on balanced diet (FLY AA, filled circles) or on severely imbalanced diets (FLY AA -80% Val, empty circles, FLY AA -90% Val, empty triangles) in germ-free (GF) condition (grey) or L. plantarum (Lp)-associated conditions (green). (elifesciences.org)
B ) Developmental timing of larvae raised on balanced diet (FLY AA, filled circles) or on imbalanced diet due to excess Val (FLY AA +60% Val, empty circles) in GF condition (grey) or Lp-associated conditions (green). (elifesciences.org)
Sequences2
In Drosophila most mature miR-10 sequences are produced from the 3' arm of the precursor while in the beetle Tribolium castaneum most production comes from the 5' arm. (wikipedia.org)
Southern blot analysis indicated that this gene shared limited nucleotide similarity with several other Drosophila genomic sequences. (marquette.edu)
Tolerance1
We previously identified the transcription factor FOXO as a regulator of hypoxia tolerance in Drosophila larvae and adults. (thegallolab.com)
Transcript2
We therefore used duox [MI11825] heterozygous mutant larvae, in which the transcript level of duox is greatly reduced (Fig. 1b ). (biomedcentral.com)
RT-qPCR analysis confirmed a significant reduction in DaOAR transcript abundance in dsRNA-fed larvae compared to controls. (biomedcentral.com)
Brain2
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophilabrain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophilabrain tissue. (sdbonline.org)
Mechanism1
This study aims to understand the function and mechanism of Drosophila Glycine N-acyltransferase (GLYAT) in cell invasion. (sdbonline.org)
Genetic1
We have initiated a genetic analysis of the Drosophila homolog of the Myc oncoprotein (dMyc),which is encoded by the dm locus. (biologists.com)
Stage2
First, during development these larvae predictably transition from living socially to solitarily, with the 3rd instar as the transition stage [ 6 ]. (biomedcentral.com)
b RT-PCR of wild-type ( w1118 ) and Duox [MI11825] heterozygous larvae. (biomedcentral.com)
Model1
Drosophila is a powerful model for the study of factors modulating innate immunity. (biomedcentral.com)
Found2
In Drosophila larvae, miR-10-3p is found in the imaginal discs (groups of cells which are destined to become adult structures upon metamorphosis). (wikipedia.org)
Some more specimens were sifted from the same plants, and larvae were found in the roots of the saxifrage. (arphahub.com)
Host1
The Drosophila renal system is an important contributor to host defense and can modulate immune responses in an organ autonomous manner, responding to environmental changes such as desiccation. (biomedcentral.com)
Response3
Here we report that duox heterozygous mutant larvae, which have normal basal nociception, exhibit an attenuated hypersensitivity response to heat and mechanical force following UV irradiation. (biomedcentral.com)
In Drosophila , the humoral immune response is characterized by the rapid induction of a battery of antimicrobial peptides (AMPs). (biomedcentral.com)
There were two main goals: (1) To determine whether feeding synthetic dsRNA to D. arcuata larvae would elicit an RNAi response. (biomedcentral.com)
Cells1
In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)
Fruit1
One of the most destructive fruit pests, its larvae feed and develop on many different fruits and some vegetables. (lookformedical.com)
Female1
Larvae were reared from eggs of female D. arcuata moths collected near Ottawa, Ontario, Canada (45.4215°N, 75.6972°W). Larvae were fed on paper birch leaves ( Betula papyrifera ) held in water-filled vials kept in glass jars at room temperature (21-23 °C) under 16 h light: 8 h dark lighting. (biomedcentral.com)
Role2
Our findings reveal a novel role for duox in nociceptive sensitization of Drosophila larvae, and will enhance our understanding of the mechanisms underlying this process in Drosophila sensory neurons. (biomedcentral.com)
Briefly, it is known that tol-1 , a homologue of the Drosophila Toll-receptor, plays a role. (biomedcentral.com)