Loading...
  • Disruption
  • Analysis and in vivo disruption of the gene coding for adenylate kinase (ADK1) in the yeast Saccharomyces cerevisiae. (mpg.de)
  • locus
  • Accordingly, we constructed a method for gene targeting in Drosophila that uses broken-ended extrachromosomal DNA molecules to produce homology-directed changes in a target locus. (sciencemag.org)
  • homeobox gene
  • During development, the homeobox gene Cdx2 exerts a homeotic function, providing the positional information necessary for correct specification of the midgut endoderm. (bmj.com)
  • The Cdx2 homeobox gene, homologous to the Drosophila gene caudal , has an essential role during early development. (bmj.com)
  • sequences
  • sequences homologous to the polypurine tract and tRNA primer binding site of retroviruses are located adjacent to the long terminal repeats. (asm.org)
  • The lethal(3)73Ah gene is located in a densely transcribed region sharing 3'-untranslated sequences with the adjacent sex-determining gene, transformer. (nih.gov)
  • pathway
  • Together, our results reveal a novel epigenetic function of dCAF-1 in promoting Notch pathway activity that regulates normal Drosophila development. (biologists.org)
  • We are using transgenic gain of function and mutagenic loss of function studies to analyze the importance of such a pathway in Drosophila . (nih.gov)
  • zinc
  • Zinc-finger and TALE nucleases proved to be extremely efficient for generating knockout and knock-in alleles compared with conventional gene targeting approaches. (biologists.org)
  • Ortholog
  • In C. elegans , two homeodomain transcription factors, TTX-3 (a LHX2/9 ortholog) and CEH-10 (a CHX10 ortholog), directly activate a large battery of terminal differentiation genes in the cholinergic interneuron AIY. (sdbonline.org)
  • ventral
  • 1992 ) Decapentaplegic acts as a morphogen to organize dorsal-ventral pattern in the Drosophila embryo. (biologists.org)
  • However, Drosophila Sog makes only minor contributions to the development of ventral structures that hypothetically correspond to the vertebrate dorsum where the axial notochord forms. (biologists.org)
  • RNA interference-mediated depletion of the spider sog gene led to a nearly complete loss of ventral structures, including the axial ventral midline and the central nervous system. (biologists.org)
  • elegans
  • We have been concentrating on the evolutionarily and medically important transition of a non-viral retrotransposon to an infectious retrovirus, using models in Drosophila and C. elegans. (searlescholars.net)
  • genomes
  • This gene has been present as a host gene in insect genomes for at least 250 million years (since the origin of Diptera) and may play a crucial role in membrane transport in female oogenesis. (searlescholars.net)
  • double-strand
  • In Drosophila , also, double-strand (ds) breaks in DNA are recombinogenic. (sciencemag.org)
  • Double-strand breaks are most frequently corrected by non-homologous end-joining (NHEJ) repair mechanisms, which can introduce small insertions or deletions (indels) at the site of repair. (biologists.org)
  • DNA ligase IV deficiency syndrome (LIG4 syndrome) is a rare autosomal recessive disorder characterized by microcephaly, growth retardation, low birth weight, dysmorphic facial findings, immunodeficiency, pancytopenia, and radiosensitivity due to impaired repair of DNA double-strand breaks by non-homologous end-joining. (biomedsearch.com)
  • sequence
  • The sequence from the iB fragment is matched against the genes from the official gene set. (uni-goettingen.de)
  • Fragile X is caused by the loss of FMR1 gene function that in most cases is due to an expansion of a trinucleotide repeat in the 5' leader sequence of the gene. (upenn.edu)
  • organisms
  • This technique may also be applicable to other organisms for which gene-targeting procedures do not yet exist. (sciencemag.org)
  • In organisms in which gene targeting has been achieved, DNA molecules with cut or broken ends have proven to be more recombinogenic than covalently closed circular DNAs ( 7 , 8 ). (sciencemag.org)
  • Gene-editing techniques are revolutionizing the way we conduct genetics in many organisms. (biologists.org)