Based on a previous report, we studied the potential involvement of the adenosine A1 receptor in the effect of CBC on these cells and found that the selective adenosine A1 receptor antagonist, DPCPX, counteracted both ERK1/2 phosphorylation and up-regulation of nestin by CBC, indicating that also adenosine is involved in these effects of CBC, but possibly not in CBC inhibitory effect on GFAP expression. (nih.gov)
DPCPX (100 mM), an adenosine (A 1 ) receptor antagonist, inhibited the inhibitory effects of both adenosine and of high concentrations of ATP. (edu.pk)
The nonselective adenosine-receptor antagonist caffeine as well as the selective A1 adenosine-receptor antagonist DPCPX however not the selective A2A adenosine-receptor antagonist SCH58261 totally avoided allopurinol-induced anti-nociception. (biopaqc.com)
The effects of adenosine, adenosine A 1 receptor antagonist (DPCPX), or NMDA receptor antagonist (APV) on the spontaneous release of [ 3 H]-5- hydroxytryptamine ([ 3 H]-5-HT) during normoxic/normoglycemic or hypoxic/hypoglycemic period were studied in the rat hippocampal slices. (ewha.ac.kr)
Adenosine A 1 receptor specific antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) exacerbate GOD-induced increase of spontaneous release of [ 3 H]-5-HT. (ewha.ac.kr)
Materials and Methods: The expression of casps3 was measured by real-time polymerase chain reaction and then flow cytometery and MTT assay were used to assess the apoptotic and proliferation cell rate after the treatment of T47D cells with specific agonist N6-cyclopentyladenosine (CPA) and antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) of this receptor 24, 48, and 72 hours after treatment. (uitm.edu.my)
Pre-treatment with pertussis toxin (Gi/o-protein inhibitor), DPCPX (A1 adenosine receptor antagonist) or TG2 inhibitors (Z-DON and R283) attenuated the A1 adenosine receptor-induced pharmacological pre- and post-conditioning. (ntu.ac.uk)
Methods: Intravital videomicroscopy of the rat jejunum was used to record the vascular responses of inflow (termed 1A) arterioles, proximal (p3A), and distal (d3A) premucosal arterioles during exposure to isotonic glucose or mannitol solutions alone or in the presence of the selective nitric oxide synthase (NOS) inhibitor (L-NMMA), an adenosine A1 receptor antagonist (8-cyclopentyl-1,3-dipropylxanthine (DPCPX)), or a K + ATP channel inhibitor (glibenclamide). (arizona.edu)
Bone marrow cells (BMCs) were harvested from C57Bl/6 female mice or A 1 R-knockout mice and their wild-type (WT) littermates and differentiated into osteoclasts in the presence of colony stimulating factor-1 and receptor activator of NF-κB ligand in the presence or absence of the A 1 R antagonist 1,3-dipropyl-8-cyclopentyl xanthine (DPCPX). (elsevierpure.com)
BMCs from A 1 R-knockout mice form fewer osteoclasts than BMCs from WT mice, and the A 1 R antagonist DPCPX inhibits osteoclast formation (IC 50 =1 nM), with altered morphology and reduced ability to resorb bone. (elsevierpure.com)
Adenosine4
The role of adenosine and the adenosine A1, A3, A2A and A2B receptors was studied by using adenosine deaminase and the selective antagonists DPCPX (1 μM), MRS 1191(1 μM), ZM241385 (1 μM) and MRS1754 (1 μM). (sun.ac.za)
Intrathecal (panels A and B) and intraplantar (panels C and D) pretreatment with 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) and the antihyperalgesic effect of 9 minutes of ankle joint mobilization (AJM) or adenosine (30 mg/kg, i.p.) in mice. (highwire.org)
Caffeine adenosineDPCPX and SCH58261 dosages had been based on previously function (Lara (1993). (biopaqc.com)
Adenosine A1 receptor blockade (DPCPX) A2a blockade (CSC) or the mKATP channel blocker 5-hydroxydecanoic acid significantly attenuated Akt activation after IPC. (researchassistantresume.com)
Selective1
Procedures -Membrane-enriched homogenates from cerebral cortex and striatum were evaluated by radioligand binding assays with the A 1 -selective ligand [ 3 H]DPCPX and the A 2a -selective ligand [ 3 H]ZM241385. (avma.org)
ZM2413851
Results -Saturable high affinity [ 3 H]DPCPX binding (A 1 ) sites were detected in cerebral cortex and striatum, whereas high-affinity [ 3 H]ZM241385 binding (A 2a ) sites were detected only in striatum. (avma.org)
Significantly2
Result: Our results indicated that DPCPX significantly induces apoptosis in T47D cells and the rate of survival cell after the reduction of this treatment, especially 72 hours after treatment. (uitm.edu.my)
Although DPCPX significantly increased the yield of repetitive stimulation in intact spinal cords, the effects of A1 antagonism on motor evoked responses after an acute spinal transection was not detected. (sissa.it)
Increase1
Conclusion: In general, DPCPX could up-regulate casp3 gene expression and subsequently increase the apoptosis rate in T47D cells with casp3 expression without the P53 gene interference. (uitm.edu.my)
Effects1
Blue bars show the effects of DPCPX (150 nmol/site, i.t. (highwire.org)