Our results indicate that primers frequently bound 1-2 bp upstream in taxa where a homopolymer region was present in the amplification direction. (nature.com)
Here we show that transcription induces stable TAF binding to downstream promoter DNA, independent of upstream contacts, TBP, or other basal transcription factors. (biorxiv.org)
The term 'CCT' typically covers every option capable of reducing emissions upstream, downstream or within the power generation process. (polity.org.za)
Proteins15
Like the other Rel proteins, NFAT5 contains the Rel homology domain, a conserved DNA-binding domain. (wikipedia.org)
RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
Receptor dimerization and autophosphorylation attracts proteins containing Src homology 2 (SH2) or phosphotyrosine binding (PTB) domains including adaptor proteins like FRS2 and GRB2. (springer.com)
however, drug-binding proteins are associated through protein-protein interaction (PPI) networks within the cell, suggesting that drug phenotypes may result from long-range network effects. (nih.gov)
We first used PPI network analysis to classify drugs based on proteins downstream of their targets and next predicted drug combination effects where drugs shared network proteins but had distinct binding proteins (e.g., targets, enzymes, or transporters). (nih.gov)
By classifying drugs using their downstream proteins, we had an 80.7% sensitivity for predicting rare drug combination effects documented in gold-standard datasets. (nih.gov)
These results demonstrate a novel paradigm for anticipating drug synergistic effects using proteins downstream of drug targets. (nih.gov)
MiRNAs are characterized by binding the 3 - UTR of special mRNA to regulate the expression of the related proteins [ 10 , 11 ]. (hindawi.com)
The Phactr family of PP1-binding proteins and the myocardin-related transcription factor family of transcriptional coactivators contain regulatory domains comprising three copies of the RPEL motif, a G-actin binding element. (rcsb.org)
RGS proteins display GAP activity towards either Gα i/o or Gα q/11 type G proteins, thereby shortening the duration that Gα is GTP bound and βγ is free. (sigmaaldrich.com)
Interestingly, recent data suggest a function for ULK1 not only during autophagy activation but also during elongation and closure of the autophagosomal membrane via binding to ATG8 proteins [ 8 ]. (hindawi.com)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
Pathways4
However, the signaling pathways downstream of p75 NTR that control growth cone functions, such as filopodial behavior, remain undefined. (jneurosci.org)
ABL1 kinase and inhibit downstream signaling pathways. (bvsalud.org)
Our results show that SLAP2 suppresses FLT3 downstream signaling pathways including AKT, ERK, p38 and STAT5 and facilitates FLT3 degradation through enhancing ubiquitination while ABL2 expression does not alter FLT3 stability or ubiquitination but partially suppresses FLT3 downstream signaling through the PI3K/AKT pathway. (lu.se)
Taken together we have shown that LTD4 might have an impact on tumour development through the regulation of CysLT1R and its downstream signalling pathways. (lu.se)
Protein kinases1
They promote cell survival by the activation of downstream protein kinases and, finally, transcription factors of the NF-kB and AP-1 family. (embl-heidelberg.de)
Kinase4
PAK, a kinase that regulates the actin cytoskeleton downstream of Rac, also binds FMRP. (fraxa.org)
Neurotrophins bind two classes of receptors, the receptor tyrosine kinase family of Trk (A, B, and C) receptors and the p75 neurotrophin receptor (NTR). (jneurosci.org)
Nerve Growth Factor (NGF) can bind to tyrosine kinase A (trkA) and with p75NTR depending the physiological state of the cell. (cdc.gov)
Using a panel of kinase inhibitors, we found ALK inhibitor AZD3463 selectively inhibited the activation and downstream signaling of FLT3-ITD and did not affect the wild-type FLT3 (FLT3-WT). (lu.se)
Receptor6
In recent years, the search for intracellular signaling integrator downstream of receptor tyrosine kinases provided valuable novel substrates. (springer.com)
Activity of these inhibitors depends on their mechanism of receptor binding (active vs inactive conformation) and efficacy against the FLT3-ITD and -TKD mutations (type 1 inhibitors are active both on FLT3 -ITD and TKD, whereas type 2 inhibitors are active only on FLT3 -ITD). (dovepress.com)
The signal imparted by the binding of a single agonist to its receptor is thus transduced and amplified leading to generation of several active αGTP and βγ molecules during the lifetime of the first αGTP. (sigmaaldrich.com)
Binding to the adapter molecule fadd recruits it to either receptor. (lu.se)
Based on binding to the androgen receptor, binding to SHBG (Sex Hormone Binding Globulin) and studies on fish, norethisterone and levonogestrel are a higher environmental risk than medroxyprogesterone. (janusinfo.se)
In order to induce its effects on the intestinal epithelial cells, LTD4 binds to its receptor CysLT1R. (lu.se)
Targets3
We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
However, little is known about the downstream targets of PITX2 in cardiogenesis. (lu.se)
Here we try to explore the possible molecular mechanism that leads to the toxicity of TiO2 and the downstream targets of NGF pathway. (cdc.gov)
Genes6
Binding to these sites consequently activates the transcription of target genes. (wikipedia.org)
The regulatory genes primarily the transcription factors that exert their activity by binding to certain cis DNA elements and consequently either up regulated or down regulate to target expression. (scielo.br)
In addition, the (pro)renin-PRR complex triggers a signal transduction cascade to mediate regulation of downstream genes independently of AngII. (mtu.edu)
TFs being expressed and the downstream differentiation target been established as candidates to regulate both pluripotency and genes being shut off [11]. (lu.se)
The student will be responsible for improving the annotations, especially for the untranslated regions that lie up and downstream of protein coding genes. (lu.se)
Effector1
Both αGTP and βγ dimers are capable of regulating downstream effector functions. (sigmaaldrich.com)
Motif1
Targeting a DNA binding motif of the EVI1 protein by a pyrrole-imidazole polyamide. (rochester.edu)
Promoter4
Furthermore, we confirmed that NRF1 directly binds to the core region of the SMAD4 promoter, thereby decreasing SMAD4 mRNA expression. (scite.ai)
TFIID binds promoter DNA to recruit RNA polymerase II and other basal factors for transcription. (biorxiv.org)
Although the TATA-Binding Protein (TBP) subunit of TFIID is necessary and sufficient for in vitro transcription, the TBP-Associated Factor (TAF) subunits recognize downstream promoter elements, act as co-activators, and interact with nucleosomes. (biorxiv.org)
This transcription-dependent TAF complex promotes subsequent activator-independent transcription, and promoter response to TAF mutations in vivo correlates with the level of downstream, rather than overall, Taf1 crosslinking. (biorxiv.org)
MRNA2
We found that nuclear respiratory factor-1 (NRF1) is a novel interaction SMAD4 partner that inhibits TGF-β/SMAD4-induced p15INK4b mRNA expression by binding to SMAD4. (scite.ai)
Several of the mRNAs regulated by FMRP directly impact spine actin dynamics, including both the mRNA encoding the Rac GTPase, a key regulator of actin dynamics, and the mRNA encoding profilin, an actin binding protein. (fraxa.org)
Regulation1
Moreover, the Targetscan, dual-luciferase reporter assay, and Western blot were used to reveal the downstream target and regulation mechanism of miR-429 in OGD-induced HBMECs. (hindawi.com)
Actin2
In the trivalent Phactr1 complex, each G-actin⋅RPEL unit makes secondary contacts with its downstream actin involving distinct RPEL residues. (rcsb.org)
Secondary actin contacts by actin-bound RPEL motifs thus govern formation of multivalent actin⋅RPEL assemblies. (rcsb.org)
Apoptotic2
It has also been reported that unprocessed NGF precursor, proNGF, binds p75NTR preferentially over trkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells. (cdc.gov)
The active dimeric enzyme is then liberated from the disc and free to activate downstream apoptotic proteases. (lu.se)
Gene4
However, the downstream transcription of the NFAT5 gene by p38 MAPK is currently not yet characterized. (wikipedia.org)
associated Gene Ontology annotation of this binding site. (ugent.be)
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. (nih.gov)
Basically, if miRNA repression is evolutionarily important for a gene, we expect the miRNA binding sites to be shared across closely related species. (lu.se)
Regulate3
Our data demonstrate that neurotrophin binding to p75 NTR is necessary and sufficient to regulate filopodial dynamics. (jneurosci.org)
1999 ) provided evidence that p75 NTR can regulate axon extension by signaling through the RhoA pathway, such that the unbound state of p75 NTR activates RhoA, whereas neurotrophin binding to p75 NTR terminates this mode of RhoA activation. (jneurosci.org)
Mechanistically, PITX2C and Nkx2.5 synergistically regulate ANF and PLOD1 expression through binding to their respective DNA elements. (lu.se)
Genetic1
Genetic variation in the ATP binding cassette transporter ABCC10 is associated with neutropenia for docetaxel in Japanese lung cancer patients cohort. (nih.gov)
Mammalian cells2
Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
The student will create a pipeline for identifying conserved miRNA binding sites across several sparrow species using TargetScan as a framework. (lu.se)
Specificity3
Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
We here investigate primer binding specificity using different primer sets from several invertebrate metabarcoding studies. (nature.com)
Table 1 The specificity of binding to different template strands for three forward and four reverse primers. (nature.com)
Pathway1
Rather, we found that phosphorylated activation loop Y842 serves as a binding site of SHP2, which is required for FLT3-induced activation of RAS/ERK pathway. (lu.se)
Subunit1
In the inactive state, GDP is tightly bound to the a subunit of the heterotrimer. (sigmaaldrich.com)
Mechanism2
I-D.hu-spring-segment-routing-proxy-forwarding ] proposes a mechanism that provides FRR protection for a binding SID of a transit node on a SR path within a single domain. (ietf.org)
It is used to explain the mechanism of SR path binding protection in single domain. (ietf.org)
Procedure1
In addition, when the node with binding SIDs fails, the procedure on each of the related node is illustrated in different cases for two different time periods. (ietf.org)
Membrane2
Meprin A and B form membrane bound homotetramer whereas homooligomers of meprin A are secreted. (embl-heidelberg.de)
PRR is a single trans-membrane protein that binds prorenin and renin with comparable affinity, facilitating local AngII generation. (mtu.edu)
Activates1
It is hypothesized that p38 MAPK phosphorylation activates c-Fos and interferon regulatory factors (IRFs), which bind to AP-1-binding sites and ISRES (Interferon Stimulated Response Element) respectively. (wikipedia.org)
Domain2
AFUA_2G16480 domain(s) with predicted zinc ion binding activity. (cdc.gov)
PGC1(1-550) was cloned downstream of the Gal4 DNA binding domain. (addgene.org)
Factor1
We recently showed that circSMARCA5 is a tumor suppressor in glioblastoma multiforme (GBM) and acts as a decoy for Serine and Arginine Rich Splicing Factor 1 (SRSF1) through six predicted binding sites (BSs). (scite.ai)
Activation1
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)
Sites4
A key component of successful COI metabarcoding primers is primer degeneracy to allow matching at variable binding sites 11 . (nature.com)
Sequence alignments built from such datasets help to identify suitable primer binding sites. (nature.com)
Matching to variable binding sites is optimized by inserting degenerate bases into the primer sequence. (nature.com)
Following TargetScan's approach, students will evaluate conservation of miRNA binding sites across annotations. (lu.se)
Found1
Additionally, we found that neurotrophin binding to p75 NTR reduces RhoA activity. (jneurosci.org)
Effects2
Page 4, Line 4: "ATSDR determined that the levels of radioactive contaminants that entered the Clinch River, and as well as those that reached the downstream Lower Watts Bar Reservoir, are too low to cause observable adverse health effects for most people who used or continue to use the river for food or recreation. (cdc.gov)
Effects on fish has been shown in various studies at levels from 1 ng/L. Based on prescribing data, expected levels downstream Swedish watewater treatment plants could be around 2.6 ng/L. (janusinfo.se)
Target1
It is well understood that homopolymer regions should be avoided for primer binding to prevent off-target amplification. (nature.com)
Activity1
Has cohesin ATPase activity, double-stranded DNA binding, topological DNA entrapment activity, role in mitotic cohesin loading and condensed nuclear chromosome, centromeric region, nuclear mitotic cohesin complex localization. (cdc.gov)
Data2
DOE's own sampling data, especially from its key fish sampling locations has been carefully archived for decades and these data confirm high levels of Cs-137 and Sr-90 and other radionuclides and fish tissue in many locations downstream of WOC. (cdc.gov)
ATSDR's failure to adequately explore the publicly available data for decades of fish tissue analyses both on the Reservation and downstream is blatantly irresponsible. (cdc.gov)
Elements1
EVI1 Interferes with Myeloid Maturation via Transcriptional Repression of Cebpa, via Binding to Two Far Downstream Regulatory Elements. (rochester.edu)
Increases1
2002 ) demonstrated that NT-3 binding to p75 NTR in cortical subplate neurons increases neurite length and filopodial formation. (jneurosci.org)
Roles1
The calmodulin-binding transcription activators (CAMTA) play critical roles in plant growth and responses to environmental stimuli. (frontiersin.org)