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  • striatum
  • Testing of F1 progeny confirmed that a single AJ chromosome had a significant effect on the rate of body weight gain and in Hdh(Q111) mice the AJ chromosome was associated subtle alterations in somatic CAG instability in the liver and the formation of intra-nuclear inclusions, as well as DARPP-32 levels, in the striatum. (nih.gov)
  • antibody
  • 2000). To assess whether the Htt CAG mutation may produce this abnormal DARPP-32 phenotype on a B6J background, we immunostained striatal sections of 5 months of age mice with DARPP-32 antibody (Fig. 7a). (nih.gov)
  • Immunohistochemistry: DARPP-32 Antibody [NB300-- Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. (novusbio.com)
  • Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.Predicted molecular weight: 32 kDa. (abcam.com)
  • glutamate
  • In the absence of Ctip2 , MSN do not fully differentiate, as demonstrated by dramatically reduced expression of a large number of MSN markers, including DARPP-32, FOXP1, Chrm4, Reelin, MOR1 (μ-opioid receptor 1), glutamate receptor 1, and Plexin-D1. (jneurosci.org)
  • pathways
  • They identify the molecular target by which THC affects sexual behavior, and unveil a remarkable operative cross talk mechanism between THC and the progesterone and dopamine signaling pathways, which were already known to play a central role in reproductive behavior. (pnas.org)
  • In addition, we use mouse models where striatal phosphoproteins and their targets have been "knocked out" to investigate the functions of these dopamine-regulated pathways in a variety of behavioral paradigms. (yale.edu)
  • extracellular
  • Carboni E, Imperato A, Perezzani L, Di Chiara G. Amphetamine, cocaine, phencyclidine and nomifensive increase extracellular dopamine concentrations preferentially in the nucleus accumbens of freely moving rats. (springer.com)
  • nucleus accumbens
  • a Micrographs of DARPP-32 positive cells in the striata and nucleus accumbens of representative 5 months mice with Hdh+/+B6J, HdhQ111/+B6J (142 CAGs), Hdh+/+B6J.AJ10 and HdhQ111/+B6J.AJ10 (140 CAGs) genotypes. (nih.gov)
  • HCN2 Channels in Cholinergic Interneurons of Nucleus Accumbens Shell Regulate Depressive Behaviors. (nih.gov)
  • p11 in Cholinergic Interneurons of the Nucleus Accumbens Is Essential for Dopamine Responses to Rewarding Stimuli. (nih.gov)
  • metabolism
  • CBD is also believed to regulate the body's metabolism of THC by inactivating cytochrome P450 , an important class of enzymes that metabolize drugs. (wikipedia.org)
  • amino
  • Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH). (novusbio.com)
  • t-Darpp is a truncated version of DARPP-32 as it is missing the first 36 amino acids at the N-terminus. (wikipedia.org)
  • postsynaptic
  • It has been predicted that DARPP-32, in conjunction with ARPP-21, could also be involved in setting-up of eligibility trace-like temporal window for striatal postsynaptic signaling. (wikipedia.org)
  • role
  • DARPP-32 plays a key role in cognitive function, and multiple brain functions. (atlasgeneticsoncology.org)
  • Dopamine plays a key role in goal-directed behavior, as well as in reward and habit learning. (yale.edu)
  • Importantly, there is now alarge body of evidence that supports a key role for DARPP-32-dependent signaling in mediating the actions of multiple drugs of abuse including cocaine, amphetamine, nicotine, caffeine, LSD, PCP, ethanol and morphine. (springer.com)
  • levels
  • DARPP-32 levels are decreased in the dorsolateral prefrontal cortex and lymphocytes of both schizophrenia and bipolar disorder patients. (wikipedia.org)
  • mice
  • HdhQ111/+ knock-in mice showed a dramatic decrease of DARPP-32 immunoreactivity when compared to wild type mice. (nih.gov)
  • cytoplasmic
  • 20220134 ). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation of delayed rectifier potassium channels. (uniprot.org)
  • plays
  • Dopamine is a simple organic chemical in the catecholamine and phenethylamine families that plays a number of important roles in the brains and bodies of animals. (definitions.net)