Donor fetal clone cells. Medical search. Frequent questions

this approach has been championed as an answer to the many issues concerning embryonic stem cells (ESCs) and the destruction of viable embryos for medical use, though questions remain on how homologous the two cell types truly are. (wikipedia.org)
Controversy surrounds human ESC work due to the destruction of viable human embryos, leading scientists to seek alternative methods of obtaining pluripotent stem cells, SCNT is one such method. (wikipedia.org)
To facilitate discussion, it was agreed to distinguish between human cloning for reproductive purposes, that is to produce a human individual, and human cloning for nonreproductive purposes, that is to produce embryos for basic and applied research. (who.int)
Some countries have proposed a total ban on any research involving the cloning of human embryos. (who.int)
Several participants reported interest among the scientific and medical communities of their countries and regions in the use of somatic cell nuclear transfer techniques to produce cloned human embryos for time-limited basic research on ageing and genetic diseases. (who.int)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
In a previous study, we found that low levels of sulphur and cobalt (low S/Co) in the diet offered to oocyte donors altered the DNA methylome of bovine embryos. (bvsalud.org)
A year ago, we showed that you could do it with cells from embryos," says Wilmut. (newscientist.com)
Particularly valuable animals could be cloned from adult cells without the uncertainties of crossing them with other animals or tinkering with embryos. (newscientist.com)
More importantly, biotechnologists will for the first time be able to manipulate the genes of cells from farm animals directly before growing them into embryos. (newscientist.com)
But scientists have not managed to isolate such cells from farm animals, and must rely instead on injecting genes randomly into early embryos. (newscientist.com)
The recent desperation to clone human embryos may be seriously undermining accepted ethical principles of medical research, with potentially profound wider consequences. (lifeissues.net)
In addition, our experiments show that heat-treating donor nuclei destabilizes higher-order features of chromatin (but leaves intact its nucleosomal organization) and results in a high proportion of reconstructed embryos developing to the blastocyst stage and beyond. (cnrs.fr)
Citizens disagree about whether we should destroy human embryos for their stem cells-and if so, which embryos, with whose money, under what regulatory guidelines. (eppc.org)
The holy grail of regenerative medicine-whatever one's ethical beliefs about destroying embryos-is to "reprogram" regular cells from one's own body so that individuals can be the source of their own rejection-proof therapies. (eppc.org)
That is to say, we risk turning developed cells into developing embryos, and thus risk engaging in the very activities of embryo destruction and human cloning that we seek to avoid. (eppc.org)
Far more controversial-and for good reason-are stem cells derived from destroyed human embryos. (eppc.org)
He also says stem cell embryos can play a role in a new life like a full-charged battery and stem cell replacement for replacing any decayed or sick cell in the body of an even 90-year-old man. (stem-cells-therapy.com)
Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. (chinagene.cn)
Aberrant methylation of donor genome in cloned bovine embryos. (chinagene.cn)
Precise recapitulation of methylation change in early cloned embryos. (chinagene.cn)
Many protocols possess been set up for sexing the cell and embryos lines in farm pets. (researchensemble.com)

In genetics and developmental biology, somatic cell nuclear transfer (SCNT) is a laboratory strategy for creating a viable embryo from a body cell and an egg cell. (wikipedia.org)
Development will ensue normally and after many mitotic divisions, the single cell forms a blastocyst (an early stage embryo with about 100 cells) with an identical genome to the original organism (i.e. a clone). (wikipedia.org)
Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning the clone embryo is implanted into a host mother for further development and brought to term. (wikipedia.org)
The aim of carrying out this procedure is to obtain pluripotent cells from a cloned embryo. (wikipedia.org)
Embryonic stem cells are undifferentiated cells of an embryo. (wikipedia.org)
However, ethical problems were foreseen with the production by cloning of fully formed and functioning organs, as participants could not envisage how such organs could be made without first producing a cloned embryo and allowing it to grow, at least partially, through the fetal stage of development. (who.int)
Nor is the embryo just a "fertilized egg", or just a "clump of cells", or appear only when the zygote is formed, or appear later after the zygote is formed, or appear after implantation - or even a week after that at 14-days. (lifeissues.net)
Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)
They produced idential lambs called Megan and Morag, which originated from different cells of the same embryo. (newscientist.com)
The latest experiments have also produced three lambs from the cells of a sheep fetus aborted after 26 days, and four from a nine-day-old embryo. (newscientist.com)
After growing and dividing for a week or so in a laboratory culture dish, the fused cell forms an early embryo called a blastocyst, which Wilmut's team implants into a surrogate mother. (newscientist.com)
During the development of vertebrates, including humans, the fertilized egg develops into the embryo, and the cells in the embryo then proceed to differentiate to form somatic cells of different tissues and organs. (shawprize.org)
The fertilized egg is considered totipotent, as it can develop into a whole organism, while the cells in the embryo are pluripotent because they are capable of differentiating into somatic cells that make up all the organs. (shawprize.org)
They pioneered a new technique of starving embryo cells before transferring their nucleus to fertilized egg cells. (shawprize.org)
Fertilization of mammalian eggs is followed by successive cell divisions and progressive differentiation, first into the early embryo and subsequently into all of the cell types that make up the adult animal. (todayinsci.com)
The first offspring to develop from a differentiated cell were born after nuclear transfer from an embryo-derived cell line that had been induced to became quiescent. (todayinsci.com)
Using the same procedure, we now report the birth of live lambs from three new cell populations established from adult mammary gland, fetus and embryo. (todayinsci.com)
Therapeutic cloning, sometimes referred to as embryo cloning, is the production of human stem cells for use in research. (mystudywriters.com)
Research advocates attack President Bush for "banning stem cell research," while pro-life advocates lament a Republican administration and Congress that have banned nothing-not embryo destruction, not human cloning, not fetal farming, not genetic engineering. (eppc.org)
Before leaving office, President Clinton sought to get around the existing law without actually changing it, by funding research on embryonic stem cells so long as the actual embryo destruction was paid for with private dollars. (eppc.org)
For the first time, scientists have created a new type of hybrid embryo in the mixture of sheep and humans, which they use for stem cells therapy. (stem-cells-therapy.com)
In view of organ donation, scientists established stem cells of the sheep in the embryo. (stem-cells-therapy.com)
Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. (chinagene.cn)
Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. (chinagene.cn)
TSA improve transgenic porcine cloned embryo development and transgene expression[J]. HEREDITAS, 2011, 33(7): 749-756. (chinagene.cn)
To usage of fetal transgenic satellite television cells for nuclear transfer Prior, sex recognition of transgenic cell lines singled out from one cell cloning is certainly required because the gender of transgenic 217087-09-7 manufacture embryo can end up being motivated by sex recognition of nuclear donor cells. (researchensemble.com)

The blastocyst stage is developed by the egg to help create embryonic stem cells from the inner cell mass of the blastocyst. (wikipedia.org)
In the United States, scientists at the Harvard Stem Cell Institute, the University of California San Francisco, the Oregon Health & Science University, Stemagen (La Jolla, CA) and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce embryonic stem cells. (wikipedia.org)
At present, this is only possible with mice, using so-called embryonic stem cells. (newscientist.com)
The new technique means they will not need embryonic stem cells. (newscientist.com)
Long before the controversy emerged over human embryonic stem cells, scientists and doctors began using first-generation stem cells from adult bone marrow. (eppc.org)
Already, non-embryonic stem cells are being used to treat a variety of diseases-most notably certain cancers of the blood. (eppc.org)
Adult stem cells are easier to control than embryonic stem cells and thus less likely to form tumors. (eppc.org)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

It is used in both therapeutic and reproductive cloning. (wikipedia.org)
CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
Finally, and inexorably, a true professional scientist poses clearly challenging questions to his research colleagues, and to the scientific enterprise in general, about the dubious "scientific" justification for the current rush to clone human beings - for both "therapeutic" and for "reproductive" purposes. (lifeissues.net)
But he is equally concerned about the unethical aspects inherent in the rush to perform " therapeutic " human cloning research, including the abuses to all vulnerable human patients who would be required to participate in clinical trials. (lifeissues.net)
Since then, the work of Wilmut and Campbell has been duplicated in many other animal species and has provided approaches to produce useful therapeutic products with cloned animals and to improve agricultural practices. (shawprize.org)
HLA-A disruption from donor cells is a step toward generating allogeneic cells as an off-the-shelf therapeutic. (ashpublications.org)
Any cell undergoing division will have an altered cytoskeleton, and the cytoskeleton itself may become a target for therapeutic agents designed to interfere with cell division (e.g., anti-cancer drugs). (openorthopaedicsjournal.com)
DNA cloning, reproductive cloning and therapeutic cloning. (mystudywriters.com)
Therapeutic cloning can be used for parts of the body containing the same set of cells. (mystudywriters.com)
But adult stem cells also raise some interesting ethical dilemmas alongside their great therapeutic promise. (eppc.org)
New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

In 1996, Dolly the sheep became famous for being the first successful case of the reproductive cloning of a mammal. (wikipedia.org)
Summary information is provided here on the outcome of the meetings held during the last three months of 1997, in which the ethical, scientific and social implications of cloning were discussed in relation to the potential biomedical applications of this technique in such areas of human health as reproductive health, xenotransplantation and medical genetics. (who.int)
Other regions reported that some individuals and religious leaders might consider reproductive cloning acceptable in certain cases such as otherwise untreatable infertility, or to avoid inherited genetic diseases. (who.int)
Last year they used the same reproductive technology to create the world's first cloned lambs (Nature, vol 380, p 64). (newscientist.com)
Agreeing with the premise of an earlier article in the same journal, he agrees that we "must not let our debate get completely derailed by vested interests, whether politically or economically motivated", and that the failure to find global agreement on human cloning at the U.N. could result in "reproductive" human cloning [and all the abuses of women that would entail]. (lifeissues.net)
Reproductive cloning was how Dolly came to earth: the nucleus of a donor adult cell is placed in an egg cell without nucleus. (mystudywriters.com)
Reproductive Cloning could, theoretically, be used for cloning humans. (mystudywriters.com)
But we can only wonder about the ethical propriety of producing the first human child with this technique, knowing that the hoped-for newborn would be a reproductive experiment, one that may end initially in numerous fetal failures. (eppc.org)

In January 2018, a team of scientists in Shanghai announced the successful cloning of two female crab-eating macaques (named Zhong Zhong and Hua Hua) from foetal nuclei. (wikipedia.org)
These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
They performed nuclear transfer experiments in which nuclei from embryonic, foetal and adult cells of the sheep were transplanted into fertilized eggs derived from ewes. (shawprize.org)
Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. (cnrs.fr)
Granulosa cells from adult sheep were heated to nonphysiological temperatures (55 degrees C or 75 degrees C) before their nuclei were injected into enucleated metaphase II oocytes. (cnrs.fr)

The birth and adult development of 'Dolly' the sheep, the first mammal produced by the transfer of a terminally differentiated cell nucleus into an egg, provided unequivocal evidence of nuclear equivalence among somatic cells. (bioscientifica.com)
Thus, Dolly was the first example of the reprogramming of the adult cell back to totipotency in a mammal. (shawprize.org)
The inventors on the '233 application, Dr. Keith H.S. Campbell and Sir Ian Wilmut, were the first to produce a cloned mammal from an adult somatic cell -- Dolly the sheep. (patentdocs.org)
164. The clone of any of claims 155-159, wherein the donor mammal is non-foetal. (patentdocs.org)
English embryologist who in 1996 supervised the team of scientists that produced a lamb named Dolly, the first mammal cloned from a cell from an adult. (todayinsci.com)

The difference with Dolly is that all her DNA originated in a cell from the udder of an adult sheep. (newscientist.com)
Dolly was the only lamb born from 277 fusions of oocytes with udder cells. (newscientist.com)
One of the live-born lambs, Dolly, was derived from the transplantation of the nucleus of an adult mammary cell. (shawprize.org)
Dr. Campbell and Sir Ian obtained U.S. Patent No. 7,514,258 for the method they used to produce Dolly: somatic cell nuclear transfer, which involves removing the nucleus of a somatic cell that has been arrested in the quiescent phase of the cell cycle and implanting that nucleus into an enucleated oocyte. (patentdocs.org)
On appeal, the Roslin Institute argued that unlike the donor sheep used to create Dolly, clones like Dolly are eligible for protection because they are 'the product of human ingenuity' and 'not nature's handiwork, but [their] own. (patentdocs.org)
The Court disagreed, stating that 'Dolly herself is an exact genetic replica of another sheep and does not possess 'markedly different characteristics from any [farm animals] found in nature,'' and thus, 'Dolly's genetic identity to her donor parent renders her unpatentable. (patentdocs.org)
There were hundreds of failed clones, several dead fetuses and horribly deformed animals before the scientists had Dolly. (mystudywriters.com)
is a British developmental biologist who was the first to use nuclear transfer of differentiated adult cells to generate a mammalian clone, a Finn Dorset sheep named Dolly, born in 1996. (mathisfunforum.com)

The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
They further created a sheep called Polly in which they showed that it was possible to incorporate a human gene into the donor's DNA before cloning, thus indicating that it may be possible to use animals to produce human proteins for the benefit of mankind. (shawprize.org)
She was special as she was the first sheep to be cloned entirely by humans. (mystudywriters.com)
The cloning of this sheep raised a lot of questions. (mystudywriters.com)
Many years of research shows that sheep will be the best donor for the human. (stem-cells-therapy.com)
But the fetal cells do not contain antigens, so through sheep stem cells therapy they will work properly and try to congregate with the human body, from where they were taken. (stem-cells-therapy.com)
He says stem cells made from human clones are much better than quality and are very effective for such treatment, even sheep stem cells therapy is also very effective. (stem-cells-therapy.com)
As this stem cells therapy will be done from our own cells or from sheep cells. (stem-cells-therapy.com)
Background This study was performed to explain the basic methods to isolate and culture of primary satellite cells (PSCs) obtained from 50 to 60-day-old sheep fetuses, single cell cloning of transfected sexing and PSCs of PSCs based on the ZFY/ZFX, amelogenin and high-motility-group (HMG) box sequences. (researchensemble.com)

The technique consists of taking an denucleated oocyte (egg cell) and implanting a donor nucleus from a somatic (body) cell. (wikipedia.org)
Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
Wilmut and his colleagues fuse the empty oocyte with the donor cell by bringing them together and subjecting them to an electric current. (newscientist.com)

The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
Every time that you are cloning a different enzyme or gene, you might have to use a different pair of restriction sites," he said. (genomeweb.com)
This study aimed to characterize the methylation pattern of the exon 10 regions of the IGF2 gene and the Imprinting Control Region (ICR) of the H19 gene in the placenta of cloned calves. (pvb.com.br)
Imagine that there will be clones, it would surely narrow down the gene diversity which we have created over the years. (mystudywriters.com)
3] Watanabe S, Iwamoto M, Suzuki SI, Fuchimoto D, Honma D, Nagai T, Hashimoto M, Yazaki S, Sato M, Onishi A. A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycin. (chinagene.cn)
Since the one cell cloning became the hurdle of making gene concentrating on duplicate, 217087-09-7 manufacture we attempted Slc2a3 to derive the transgenic cell lines from satellite television cells transfected with pEGFP-N1 plasmid as a model of transgenic satellite television cell. (researchensemble.com)

Through somatic cell nuclear transfer, we finally obtain transgenic cattle with increased resistance to tuberculosis. (biomedcentral.com)
It is, however, important to distinguish the use of bST from other biotechnologies, such as transgenic or cloned animals. (nationalacademies.org)
Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. (chinagene.cn)
Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent protein. (chinagene.cn)
Cloned transgenic swine via in vitro production and cryopreservation. (chinagene.cn)
Latest research have got demonstrated that fetal skeletal muscles satellite television cells possess a versatile potential to end up being utilized for transgenic pet creation by somatic cell nuclear transfer technique because these cells are muscle-derived control cells that can possibly expand and differentiate. (researchensemble.com)
The upcoming objective is certainly to apply these cells for the creation of transgenic lamb by somatic cell nuclear transfer technique. (researchensemble.com)

EBV-transformed B cells (EBV-LCL) were cultured in IMDM (Cambrex, Verviers, Belgium) with 10% Fetal Bovine Serum (FBS). (haematologica.org)
T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)
In PRA testing, recipient serum is incubated with white blood cells pooled from a group of blood donors with human leukocyte antigen (HLA) types representative of the community. (medscape.com)
B19 was discovered in England in 1975 in serum specimens from healthy blood donors (1). (cdc.gov)

Because hydrostatic pressure can be a major factor in chondroinduction of mesenchymal stem cells, it was proposed to investigate the importance of the cytoskeleton in transduction of hydrostatic pressure by C3H10T1/2 murine fibroblasts, a model of primary bone marrow mesenchymal stem cells. (openorthopaedicsjournal.com)
Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)

Similarly, there was interest in using the procedure to produce cloned tissue and organs for possible future transplantation in the nuclear donor and perhaps other tissue- compatible recipients. (who.int)
Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
If the same could be achieved in humans, it would mean that each of us could have clones of ourselves made from our own tissue. (newscientist.com)
Mammary glands are rich in these cells, which are more adaptable than other tissue. (newscientist.com)
There are however a lot of risks such as the fetal tissue dying, or tissue rejection if you actually create an organ. (mystudywriters.com)
Mesenchyme is a type of animal tissue comprised of loose cells embedded in a mesh of proteins and fluid, called the extracellular matrix. (asu.edu)
and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)
Most stem cells intended for regenerative therapy and generally isolated either from the patient's bone marrow or from adipose tissue. (stem-cells-therapy.com)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

Peripheral blood mononuclear cells (PBMC) were collected from healthy donors after approval by the LUMC institutional review board and informed consent according to the Declaration of Helsinki. (haematologica.org)
T cells were isolated directly from anticoagulated peripheral blood obtained from healthy donors by negative immunomagnetic selection. (nih.gov)
Furthermore, in vitro stimulation of PBMC from 6 healthy donors with overlapping peptides from MELOE-1 or MELOE-3 revealed a very scarce MELOE-3 specific T cell repertoire as compared to the abundant repertoire observed against MELOE-1. (oncotarget.com)

Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)

In this respect, the choice of the targeted antigens remains a critical issue [ 2 ] and the ideal antigen(s) should have the following characteristics : be as tumor specific as possible and stimulate a broad T cell repertoire in the majority of patients (i.e. generate many epitopes in various HLA contexts). (oncotarget.com)

Potential effects of forced CD20 expression on T-cell function were investigated by comparing CD20- and mock-transduced cytomegalovirus (CMV) specific T cells for cytolysis, cytokine release and proliferation. (haematologica.org)
During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
Cell surface expression of DAbR1 was confirmed by cell uptake studies with radiolabeled AABD. (snmjournals.org)
Quantitative RT-PCR and in situ hybridization detected the expression of PRL not only in Leydig cells but also in germ cells, in particular in spermatogonia. (go.jp)
Allogeneic-donor-derived cells can be genetically modified to eliminate expression of HLA-A. (ashpublications.org)
Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)
Quantitative mRNA analysis wasused to determine whether G-CSF mobilizes Hox11+ stem cells and whether expression occurs in a cell populationdistinct from CD34+ cells. (fliphtml5.com)
NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. (bdbiosciences.com)

Somatic cell nuclear transfer is a technique for cloning in which the nucleus of a somatic cell is transferred to the cytoplasm of an enucleated egg. (wikipedia.org)

These cells are deemed to have a pluripotent potential because they have the ability to give rise to all of the tissues found in an adult organism. (wikipedia.org)
Another application of SCNT stem cell research is using the patient specific stem cell lines to generate tissues or even organs for transplant into the specific patient. (wikipedia.org)
No ethical problems were envisaged with the use of somatic cell nuclear transfer techniques which would lead directly to cloned differentiated cells or tissues such as skin, for future use by the nuclear donor. (who.int)
Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
Combined with the incorporation of new tools for genetic modification, these novel techniques promise to (i) transform and accelerate our understanding of genetic diseases and the development of targeted therapies through creation of tailored animal models, (ii) provide safe animal cells, tissues and organs for xenotransplantation, (iii) contribute to the preservation of endangered species, and (iv) improve global food security whilst reducing the environmental impact of animal production. (bioscientifica.com)
Imaging studies showed 86 Y-AABD was retained by DAbR1-positive T cells while it continuously cleared from normal tissues, allowing for in vivo tracking of intravenously administered CAR T cells. (snmjournals.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Today, we can derive stem cells from a range of adult and newborn tissues: liver cells, kidney cells, brain cells, fat cells, and umbilical cord blood. (eppc.org)
Also, satellite television cells offer a steady model for tissues system research, such as those regarding the transplantation of muscle-derived satellite television cells for muscles tissues renovation [2]. (researchensemble.com)
Among these features are the peculiar tropism of the virus for Vero cells (a continuous cell line established from monkey kidney epithelial cells), its capacity for growth at 37°C (while other respiratory coronaviruses grow at lower temperatures), and its ability to infect lower respiratory tract tissues ( 13 ). (cdc.gov)

Somatic cell nuclear transplantation has become a focus of study in stem cell research. (wikipedia.org)
4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
These animals were studied between 7 and 12 mo after transplantation, at which times all thymocytes and peripheral T cells were derived from stem cells of the fetal liver graft. (rupress.org)
Nuclear transplantation is a method in which the nucleus of a donor cell is relocated to a target cell that has had its nucleus removed (enucleated). (asu.edu)
Nuclear transplantation, as it was first called, was later referred to as somatic nuclear transfer or cloning. (asu.edu)
A consequence of the latter cell flow was recently demonstrated in a clinical study in cord blood transplantation. (ashpublications.org)
The percentage of the donors against which the recipient reacts is used as a predictor of the likelihood of a positive cross-match that would prevent transplantation. (medscape.com)

We identify the main binding sites of a catalytically inactive Cas9 (dCas9) protein in bovine fetal fibroblast cells (BFFs) with chromatin immunoprecipitation sequencing (ChIP-seq). (biomedcentral.com)

T cells were then cultured for 7 days at 37°C in an 8% CO2 atmosphere with various cytokines to induce their polarization in Th1 and Th17 cells. (nih.gov)
Human hematopoietic cells and thymic epithelial cells induce tolerance via different mechanisms in the SCID-hu mouse thymus. (rupress.org)
These data demonstrate that T cells become tolerant to autologous and allogeneic HLA antigens expressed in the thymus via two different mechanisms: hematopoietic cells present in the thymus induce tolerance to "self"-antigens by clonal deletion, whereas thymic epithelial cells induce tolerance by clonal energy and possibly deletion of high affinity clones. (rupress.org)
For induce splenomegaly in most donors and in rare, severe cases splenicautologous stem cell transplants, the use of autologous PBSCT from rupture [13,14]. (fliphtml5.com)
Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)

In human SCNT experiments, these eggs are obtained through consenting donors, utilizing ovarian stimulation. (wikipedia.org)
The second being a somatic cell, referring to the cells of the human body. (wikipedia.org)
Only a handful of the labs in the world are currently using SCNT techniques in human stem cell research. (wikipedia.org)
The Special Programme of Research, Development and Research Training in Human Reproduction organized a second interregional and interdisciplinary meeting on cloning (Geneva, 24 October 1997), in conjunction with a regular session of its Scientific and Ethical Review Group. (who.int)
The participants reviewed information from their organizations, countries and regions, which included statements made by governments and professional societies, general articles, and reports of official meetings and public debates, illustrating attitudes and responses to the potential uses of cloning in the area of human health. (who.int)
It appeared that the nature and extent of public information and debate on cloning and its potential advantages and disadvantages in the area of human health varied around the world. (who.int)
We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
The real experts to ask about the accurate scientific facts of human embryology are the scientific experts in human embryology who are academically credentialed Ph.D. human embryologists - not the "experts" in cell biology, genetics, doctors, nurses, theologians, lawyers or politicians, secretaries, news journalists, etc. (lifeissues.net)
While it is banned in Britain, however, human cloning is legal elsewhere, including the US. (newscientist.com)
Ethically, since eventually all such "research" will be applied to people, he cautions against the abuse of women "egg" donors, and against the premature use of vulnerable sick human patients for testing supposedly "patient-specific" stem cells in supposed "therapies", pointing to the obvious violations of standard international research ethics guidelines such clinical trials would necessarily entail. (lifeissues.net)
And he also agrees that if we don't find global agreement on human cloning, "we can probably expect dire consequences for the future of biomedical research and its impact on society at large. (lifeissues.net)
As he has questioned the HFEA before, would not the use of vulnerable human patients in clinical trials be premature, dangerous, and unethical given the already acquired knowledge in the research community that such supposed "patient-specific" stem cells would most probably cause serious immune rejection reactions in these patients? (lifeissues.net)
We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
Attempts were then made to show that mammalian cells - and human cells in particular - could also be reprogrammed back to a pluripotent state, because it is believed that such knowledge may advance our understanding of developmental mechanisms, and yield new approaches for disease treatment. (shawprize.org)
The scientists honoured by the 2008 Shaw Prize in Life Science and Medicine used different approaches to reprogramme an adult cell into the totipotent or pluripotent state, and in doing so made important contributions to potential new approaches to improve agriculture practices and to treat human diseases. (shawprize.org)
For this step, the EasySep™ Direct Human T Cell Isolation Kit was used according to the manufacturer's instructions under sterile conditions and at room temperature. (nih.gov)
DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. (snmjournals.org)
We demonstrate this approach on simulated data and experimental data of the kinase Csk and the adaptor PAG in primary human T cell immunological synapses. (nature.com)
To study the role of thymic education on the development of the human T cell repertoire, SCID-hu mice were constructed with fetal liver and fetal thymus obtained from the same or two different donors. (rupress.org)
Limiting dilution analysis revealed that T cells reacting with the human histocompatibility leukocyte antigens (HLA) of the FLD were undetectable in the CD8+ T cell population and barely measurable in the CD4+ subset. (rupress.org)
This isn't used at all to create cloned human beings, it's just for the research because Stem cells are quite important. (mystudywriters.com)
It's not possible to clone entire human beings as there is little known about cloning and the human body is just far to complex to be created in a laboratory. (mystudywriters.com)
So most scientists agree that it is not possible yet to clone entire human beings. (mystudywriters.com)
Most of these scientists highly doubt that it would be possible in the near future to clone entire human beings, but there are always the "what if" questions. (mystudywriters.com)
These are most what if questions and to be quite honest I believe that we shouldn't clone human beings. (mystudywriters.com)
In July 2005, for example, scientists announced that they had engineered adult mouse stem cells into usable mouse eggs, a technique that might one day allow for the creation of human eggs from ordinary human cells. (eppc.org)
The product is a sterile, frozen solution of pooled human plasma from several donors. (medscape.com)
In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
The CD5 antibody, clone L17F12, is derived from the hybridization of NS-1/Ag4 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human T-acute lymphoblastic leukemia (ALL) cells. (bdbiosciences.com)
A major lymphoid subpopulation in human fetal spleen: phenotypic and functional studies. (bdbiosciences.com)
Phenotypic and functional characterization of human Leu-1 (CD5) B cells. (bdbiosciences.com)
Hardy RR, Hayakawa K, Shimizu M, Yamasaki K, Kishimoto T. Rheumatoid factor secretion from human Leu-1+ B cells. (bdbiosciences.com)
Human T-cell antigens deﬁned by monoclonal antibodies: The 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. (bdbiosciences.com)
This report* was developed to assist physicians, public health officials, and other health-care professionals respond to public concerns about recently recognized, serious complications of human parvovirus B19 (B19) infection, including transient aplastic crisis (TAC), chronic anemia, and fetal death. (cdc.gov)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)

At day 35, the previously polarized Th1 and Th17 cells were seeded to a total of 0.5 x 106 T cells per sheet on half of the dermal fibroblast sheets, with a ratio of 1:1. (nih.gov)
At day 35, a fibroblast sheet with keratinocytes was superimposed on a T cell-containing fibroblast sheet, thus forming a skin substitute comprising a T-cell-enriched dermis and an epidermis. (nih.gov)

May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. (lu.se)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
This ground-breaking experiment challenged a long-standing dogma of irreversible cellular differentiation that prevailed for over a century and enabled the development of methodologies for reversal of differentiation of somatic cells, also known as nuclear reprogramming. (bioscientifica.com)
The fact that a lamb was derived from an adult cell confirms that differentiation of that cell did not involve the irreversible modification of genetic material required far development to term. (todayinsci.com)
10 MPa) stimulates chondrogenic differentiation of mesenchymal stem cells, but mechanotransduction mechanisms are not well understood. (openorthopaedicsjournal.com)
In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)

Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
A panel of DAbR1-expressing T cells and CAR T cells exhibited greater than 8-fold increased uptake of 86 Y-AABD in vitro when compared with nontransduced cells. (snmjournals.org)
Data from in vitro translation experiments and transfection of melanoma cells with bicistronic vectors documented that MELOE-3 is exclusively translated by the classical cap-dependent pathway. (oncotarget.com)
Forty-two of 104 tetramer pos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. (ashpublications.org)
To gain insight into SARS-CoV biopathology, we analyzed the relevant features of SARS-CoV HSR1 growth in vitro, including the ultrastructural analysis of the consequences of virus replication in Vero cells. (cdc.gov)

These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
Viable Offspring Derived from Petal and Adult Mammalian Cells', Nature (1997), 385 , 810. (todayinsci.com)

Peripheral T cell lymphomas (PTCLs) are generally chemotherapy resistant and have a poor prognosis. (mdpi.com)
A stage II trial of AUY922 can be open up in GIST [98] and a stage II trial has been approved but is not yet active for individuals with diffuse large B-cell lymphoma or peripheral T-cell lymphoma [99]. (forgetmenotinitiative.org)
Citation: Mera T, Heimfeld S, Faustman DL (2014) The Spleen Contributes Stem Cells to Peripheral Blood Stem Cell Transplants. (fliphtml5.com)
Samples of peripheral blood lymphocytes (PBLs) were obtained from ten normal untreateddonors and 18 normal donors treated with G-CSF. (fliphtml5.com)
G-CSF was found to mobilize both CD34+ stem cells (p=0.02)and even more dramatically mobilize Hox11+ splenic stem cells (p=0.000013) into the peripheral blood. (fliphtml5.com)
TheIntroduction later step is unique to splenic function since splenectomy results in similar accumulations of naïve B cells, reduction of memory B cells and Treatment of malignancies with allogeneic peripheral blood stem well-known susceptibilities to select infections [12]. (fliphtml5.com)
Interestingly, thiscell transplants (PBSCTs) from donors given granulocyte-colony- immature peripheral phenotype was similar to bone marrow transplantsstimulating-factor (G-CSF) has decreased relapse rates and improved before G-CSF. (fliphtml5.com)
Peripheral blood mononuclear cells (PBMCs) were used to measure the enzymatic activities of citrate synthase and complexes I, II, and IV and mtDNA copy number. (bvsalud.org)

In 1995, they produced a pair of lambs called Megan and Morag from embryonic cells. (shawprize.org)
The birth of lambs from differentiated fetal and adult cells also reinforces previous speculation that by inducing donor cells to became quiescent it will be possible to obtain normal development from a wide variety of differentiated cells. (todayinsci.com)

These cells genetically matched the donor organism from which they came. (wikipedia.org)

Adipose stem cells in reparative goat mastitis mammary gland. (sciendo.com)

Therefore, we co-cultured AML cells with macrophages. (frontiersin.org)
Macrophages are the most abundant white blood cells in the immune microenvironment and have a high degree of heterogeneity. (frontiersin.org)
Dendritic cells and macrophages of fetal liver donor (FLD) origin were abundantly present in the medullary and cortico-medullary areas. (rupress.org)

Half a century ago, it was found by John Gurdon that this developmental clock can be reversed, and that differentiated somatic cells in a frog model could regain their pluripotency or totipotency. (shawprize.org)

Nor do only the cells of the inner cell mass of the blastocyst become the later adult and none of the cells from the inner cell mass become part of the placenta, umbilical cord, etc. (lifeissues.net)

Chimeric antigen receptors (CARs) endow T cells with antigen-specific recognition, activation, and proliferation independent of major histocompatibility complex ( 1 - 3 ). (snmjournals.org)
Splenomegaly might reflect dramatic G-CSF-inducedG-CSF stimulation also in multiple studies shows faster recovery of Hox11+ stem cell proliferation. (fliphtml5.com)

They fuse as one cell," says Wilmut. (newscientist.com)
Wilmut says there were so many failures because it is difficult to ensure that the empty oocyst and the donor cell are at the same stage of the cell division cycle. (newscientist.com)
The technique synchronized the cell cycles of both cells and the results led Wilmut and Campbell to believe that any type of cell could be used to produce a clone. (shawprize.org)

The genetic material of the donor egg cell is removed and discarded, leaving it 'deprogrammed. (wikipedia.org)
Next, the researchers take cells containing donor genetic material. (newscientist.com)

First, they extract immature, unfertilised egg cells called oocytes from the ovaries of ewes. (newscientist.com)

Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)

The disease specific stem cell lines could then be studied in order to better understand the condition. (wikipedia.org)
Stem Cell Res. (sciendo.com)
In addition, Dolly's DNA may have come from a stem cell that had not yet matured into an udder cell. (newscientist.com)
J Stem Cell Res Ther 4: 253. (fliphtml5.com)
Stem Cell Mera et al. (fliphtml5.com)
The findingssupport the hypothesis that G-CSF mobilizes two distinct stem cell populations, one from the bone marrow andthe other from the spleen. (fliphtml5.com)
Our hypothesis about a splenic stem cell contributionor maintained survival compared to bone marrow transplants, to PBSCT also derives from the observation that G-CSF mobilizationsalthough graft versus host disease (GVHD) still occurs [1]. (fliphtml5.com)
This is our sad state of stem cell affairs. (eppc.org)
But if we are to make wise policy the stem cell/cloning arena, we need to step back, sort out the various scientific alternatives and moral issues, and search for a way forward that all citizens can embrace. (eppc.org)
To this end, we offer a detailed analysis of the stem cell/cloning question-where is the science, what are the political alternatives, and what moral obligations should guide us? (eppc.org)
Last May, the House of Representatives passed a bill to create a cord blood stem cell bank, legislation that is likely to become law with virtually unanimous support. (eppc.org)
These moral perils are surely not a reason to oppose adult stem cell research, which deserves vigorous and expanded public support. (eppc.org)
Stem cell system is new hope lighting for medical science. (stem-cells-therapy.com)
Through stem cell, we will able to recover many parts of our body and also will able to get rid of many diseases without any side effects. (stem-cells-therapy.com)
Great Iranian Muslim scholars netics, stem cell research, and organ trans- laid huge emphasis on teaching and practis- plantation are some of the medical issues ing ethics. (who.int)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)

Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

DNA cloning or recombinant DNA technology is to transfer one piece of DNA into something that can duplicate himself. (mystudywriters.com)

You could for instance and theoretically seen once again, clone a kidney. (mystudywriters.com)

In the field of cancer immunotherapy, the recently described effectiveness of checkpoint inhibitors such as anti-PD1 blocking antibodies to boost anti-tumor T cell responses is very encouraging [ 1 ]. (oncotarget.com)
Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. (bdbiosciences.com)
Warnke RA, Levy R. Detection of T and B cell antigens with hybridoma monoclonal antibodies: a biotinavidin-horseradish peroxidase method. (bdbiosciences.com)
Cell kill indicates that the recipient has antibodies against the donor cells. (medscape.com)

Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
Thymocytes of SCID-hu mice transplanted with liver and thymus of two different donors (FLDA/FTDB animals) were nonresponsive to Epstein-Barr virus-transformed B cell lines (B-LCL) established from both the FLDA and FTDB, but proliferated vigorously when stimulated with third-party allogeneic B-LCL. (rupress.org)

We hypothesize that the reason why CD34+-enriched PBSCTsare not more effective is because the enrichment and purification process leaves out G-CSF-mobilized stem cells fromanother source, the spleen, which holds a unique reservoir of Hox11+ stem cells. (fliphtml5.com)
The spleen also uniquely contributes toGraft Versus Host Disease (GVHD) complete B cell memory [11]. (fliphtml5.com)
The stem cells of the spleen allow for full maturation of immature transitional B cells into naive B cells. (fliphtml5.com)

Review of Critical Article: Cobbe, 'Why the apparent haste to clone humans? (lifeissues.net)
See Neville Cobbe, "Why the apparent haste to clone humans? (lifeissues.net)
Now you may ask what this has to do with the whole "can we clone humans" and the ethical and moral issues. (mystudywriters.com)
Can we clone humans using the DNA cloning? (mystudywriters.com)
The benefits of cloning (parts of) humans being that we wouldn't need any more donors, or we could do research on organs without having to use humans themselves. (mystudywriters.com)
Scientists believe that it can be possible by using stem cells as a substitute for humans. (stem-cells-therapy.com)

G-CSF mobilizesCD34+ hematopoietic stem cells from bone marrow into the blood. (fliphtml5.com)

1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)

The lack of targeted immunotherapeutic approaches for T cell malignancies results in part from potential risks associated with targeting broadly expressed T cell markers, namely T cell depletion and clinically significant immune compromise. (mdpi.com)
Successful treatment of B-cell malignancies with CD19-CAR T cells was a milestone for this approach ( 4 , 5 ). (snmjournals.org)

Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
Transfer of HSV-tk to DLI preserves the beneficial anti-tumor effect and allows in vivo elimination of donor T cells if severe GvHD occurs. (haematologica.org)
In addition, the feasibility of imaging of DAbR1-positive T cells in vivo after intravenous injection of 86 Y/ 177 Lu-AABD was studied and radiation doses determined. (snmjournals.org)
Selective T cell ablation in vivo with 177 Lu-AABD seems feasible for clustered T-cell populations. (snmjournals.org)
We have demonstrated for the first time that T cells can be modified with DAbR1, enabling their in vivo tracking via PET and SPECT. (snmjournals.org)
Therefore, ex vivo NK cell expansion protocols are currently being developed. (frontiersin.org)
In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)

The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)
They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)

The blastocyst can then be transferred to a recipient (h) and cloned animals are born after completion of gestation (i). (biomedcentral.com)

Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)

As an example, Vector NTI identifies restriction sites in a plasmid, which works well for the more traditional methods of assembling DNA, such as PCR, but Hillson notes that newer tools like SLIC and the Gibson method don't use restriction enzymes or multiple cloning sites in their protocols. (genomeweb.com)
B)The use of BACs reduces the frequency with which specific genes will be cut within the coding region by restriction enzymes and divided up among two or more clones. (easynotecards.com)

The resulting cells would be genetically identical to the somatic cell donor, thus avoiding any complications from immune system rejection. (wikipedia.org)
The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors. (ashpublications.org)

B19 has also been associated with fetal death (both spontaneous abortions and stillbirths), acute arthralgias and arthritis, and chronic anemia in immunodeficient patients (5-14). (cdc.gov)

This gives them the ability to create patient specific pluripotent cells, which could then be used in therapies or disease research. (wikipedia.org)
In addition, the possibility of reprogramming adult stem cells back to a "pluripotent" (or embryonic-like) state raises the biological prospect of going back too far. (eppc.org)

However, a significant number of patients are non responders to these therapies and thus, there is still room for improvement using antigen-specific immunotherapy, whether through vaccination or through T cell transfer. (oncotarget.com)

Anatomy25

Organisms14

Diseases10

Chemicals and Drugs51

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Psychiatry and Psychology1

Phenomena and Processes61

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Named Groups3

Health Care7

Embryos23

Embryo26

Embryonic stem8

Therapeutic13

Reproductive8

Nuclei5

Mammal5

Dolly8

Sheep10

Oocyte4

Gene9

Transgenic7

Serum4

Fibroblasts3

Tissue13

Healthy donors3

Fetuses1

Targeted antigens1

Expression15

Nucleus of a somatic cell1

Tissues11

Transplantation7

Chromatin1

Induce5

Human35

Genetically modified1

Fibroblast2

Differentiation7

Vitro6

Mammalian cells2

Peripheral8

Lambs2

Organism1

Mammary1

Macrophages3

Developmental1

Placenta1

Proliferation2

Wilmut3

Genetic material2

Oocytes1

Subpopulation1

Stem cell16

Autologous4

Recombinant1

Kidney1

Antibodies5

Allogeneic2

Spleen3

Humans6

Bone marrow1

Malignant cells3

Malignancies2

Graft2

Vivo9

Tumor cells2

Recipient1

Mesenchymal stem1

Restriction enzymes2

Immune2

Acute1

Pluripotent2

Antigen-specific1