Donor egg cell somatic cell clone cells. Medical search. Frequent questions

The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
It is used in both therapeutic and reproductive cloning. (wikipedia.org)
Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning the clone embryo is implanted into a host mother for further development and brought to term. (wikipedia.org)
SCNT, or therapeutic cloning, is one method used to produce a source of individually-tailored stem cells. (medicalxpress.com)
If the cloned human organism is to be experimented upon and destroyed, the process is often called "therapeutic cloning. (cbc-network.org)
A number of large biotech companies and scientists are looking toward stem cells as the basis for a therapeutic solution to cure such illnesses as blindness, diabetes and spinal cord injuries. (thefutureofthings.com)
Therapeutic cloning refers to the production of embryonic stem cells for medicinal reasons, for example regenerative medicine and tissue replacement. (geminigenetics.com)
Therapeutic cloning involves the creation of an early-stage embryo (blastocyst) and the removal of stem cells from the developing embryo. (geminigenetics.com)
In therapeutic cloning, the blastocyst is not transferred to a womb. (eurostemcell.org)
Another long-term hope for therapeutic cloning is that it could be used to generate cells that are genetically identical to a patient. (eurostemcell.org)
To date, no human embryonic stem cell lines have been derived using therapeutic cloning, so both these possibilities remain very much in the future. (eurostemcell.org)
One of the next preclinical steps, according to the authors, is to evaluate, in the lab, differentiated patient-specific human embryonic stem cell lines for immune-system tolerance, therapeutic efficacy and safety. (scienceblog.com)
A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
One of the most promising techniques is what is known as therapeutic cloning. (spiked-online.com)
Otherwise, such a treaty would not recognize the inherent human nature of the early human embryo or fetus until after birth , and thus cloning them and using them for research - both "therapeutic" and "reproductive" -- would not be banned, and women undergoing "infertility treatments" could surely be put in danger. (lifeissues.net)
The alleged need for so-called therapeutic cloning - cloning embryos for research - is now passé. (bioedge.org)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
One important distinction is that while the method might be considered a technique for cloning stem cells, commonly called therapeutic cloning, the same method would not likely be successful in producing human clones otherwise known as reproductive cloning. (ohsu.edu)

One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
In genetics and developmental biology, somatic cell nuclear transfer (SCNT) is a laboratory strategy for creating a viable embryo from a body cell and an egg cell. (wikipedia.org)
In human SCNT experiments, these eggs are obtained through consenting donors, utilizing ovarian stimulation. (wikipedia.org)
Controversy surrounds human ESC work due to the destruction of viable human embryos, leading scientists to seek alternative methods of obtaining pluripotent stem cells, SCNT is one such method. (wikipedia.org)
For example, if a person with Parkinson's disease donated their somatic cells, the stem cells resulting from SCNT would have genes that contribute to Parkinson's disease. (wikipedia.org)
Another application of SCNT stem cell research is using the patient specific stem cell lines to generate tissues or even organs for transplant into the specific patient. (wikipedia.org)
Only a handful of the labs in the world are currently using SCNT techniques in human stem cell research. (wikipedia.org)
Somatic cell nuclear transfer (SCNT) is a type of cloning that has to be done in a lab. (bartleby.com)
In SCNT they take the nucleolus out of an egg cell, replace it with the nucleolus of a somatic cell (body cell with two complete sets of chromosomes), and make the egg cell divide into a blastocyst ("What Is Cloning? (bartleby.com)
Scientists from the Monash Institute of Medical Research (MIMR) and colleagues from New South Wales will compare two different methods of creating patient-specific stem cells: somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPS). (medicalxpress.com)
One aspect to this project will be to source oocytes, or immature egg cells to generate SCNT embryos from which embryonic stem cells are harvested. (medicalxpress.com)
Unlike SCNT, iPS cells are derived from adult cells, such as skin, which are reprogrammed to behave like embryonic stem cells. (medicalxpress.com)
Once our team produces the iPS cells, we will compare the properties of iPS versus cells developed through SCNT. (medicalxpress.com)
Australia is already a global leader in overall stem cell research and this new and clear regulatory framework gives us an opportunity to extend our leadership into SCNT which could transform how we treat diabetes, heart diseases and Parkinson's," Mr Jennings said. (medicalxpress.com)
The primary cloning technique is called "somatic cell nuclear transfer" (SCNT). (cbc-network.org)
If the authors of this bill really meant what they appear to have written, their legislation would ban all human cloning, since as we have seen, biologically, a new human organism, that is, a new human being, comes into existence with the completion of SCNT. (cbc-network.org)
This is junk biology since implanting isn't the act of asexual reproduction: SCNT cloning is. (cbc-network.org)
Indeed, if passed, Hatch/Feinstein/Kerry would explicitly legalize doing in humans the very cloning procedure -- somatic cell nuclear transfer (SCNT) -- that was used to make Dolly the sheep . (lifeissues.net)
Comment: Indeed, if passed, "total cloning bans" H.R. 534, H.R. 234, H.R. 916, and S. 245 would not ban anything either - not even the SCNT cloning technique that was used to make Dolly the sheep. (lifeissues.net)
Using eggs from adult women who had previously donated for successful fertility treatments, the researchers used SCNT to transfer DNA into the egg cells. (the-scientist.com)
Cloning, or somatic cell nuclear transfer (SCNT), is the technique used to produce Dolly the sheep, the first animal to be produced as a genetic copy of another adult. (eurostemcell.org)
This method is called "somatic cell nuclear transfer" or SCNT. (scienceblog.com)
Last week's newspapers carried the news of what was widely described as a significant "breakthrough" in stem cell science: the first successful human use of a technology known as Somatic Cell Nuclear Transfer (also referred to as SCNT). (intlstemcell.com)
I realize that I could be accused of bias because I am one of the founders of International Stem Cell, but, in fact, our company also owns license rights to some of the key intellectual property that is required to create cells through SCNT technology and our scientists are very familiar with its promise and its limitations. (intlstemcell.com)
Like embryonic stem cells and SCNT cells, these cells can be converted into almost any cell in the human body and thus have enormous potential for human therapy. (intlstemcell.com)
Unlike SCNT cells, parthenogenetic stem cells require no genetic manipulation or insertion of foreign DNA. (intlstemcell.com)
The cell lines that are produced from this method, unlike cell lines from embryonic stem cells or from SCNT, can potentially be matched to millions of people in the same way that an organ transplant is matched between donor and patient. (intlstemcell.com)
Again, Saunders is referring to SCNT as "THE" cloning procedure, when there are many other ways to clone a human being as well, and he is scientifically mis-defining the product of SCNT (i.e., the cloned human embryo). (lifeissues.net)
Dolly was the first mammal cloned from specialized adult (somatic) cells with the technique of somatic cell nuclear transfer (SCNT). (asu.edu)
The technique used by Drs. Mitalipov, Paula Amato, M.D. , and their colleagues in OHSU's Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, is a variation of a commonly used method called somatic cell nuclear transfer, or SCNT. (ohsu.edu)
Here's what I mean: Each try at somatic cell nuclear transfer (SCNT) cloning to manufacture a human being (or, member of the species Homo sapiens , if you prefer) requires a human egg. (cbc-network.org)
There are enough for IVF, but way too few for the hundreds of thousands needed for the extensive trial and error process that will be required to prefect human SCNT, at least to develop the cloned embryo to the point where stem cells can be harvested and eventually, cloned embryos eventually gestated to birth. (cbc-network.org)

Development will ensue normally and after many mitotic divisions, the single cell forms a blastocyst (an early stage embryo with about 100 cells) with an identical genome to the original organism (i.e. a clone). (wikipedia.org)
The aim of carrying out this procedure is to obtain pluripotent cells from a cloned embryo. (wikipedia.org)
Embryonic stem cells are undifferentiated cells of an embryo. (wikipedia.org)
It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
A little shot of electricity comes next, and if all goes well, a new human cloned embryo comes into being and begins to develop in the same way as a sexually created embryo. (cbc-network.org)
Cloning of a human being" means asexual reproduction by implanting or attempting to implant the product of nuclear transplantation [e.g., an embryo] into a uterus or substitute for a uterus with the purpose of producing a human being. (cbc-network.org)
Park Se-pill, head of Seoul-based fertility clinic Maria Biotech, said the tests can silence Hwang's critics who have suggested the dog might be a twin created from a split embryo rather than a clone. (blogspot.com)
The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
The resulting embryo is then implanted into a surrogate mother, resulting in the birth of an animal genetically identical to the body cell donor. (geminigenetics.com)
An electrical impulse is then applied to the egg cell to stimulate it to become an embryo. (geminigenetics.com)
Though pet cloning may be considered a relatively new technology, the process of cloning as defined above is first documented in 1885, where Hans Adolf Eduard Driesch demonstrated artificial embryo twinning on a sea-urchin. (geminigenetics.com)
… "embryo" means a human organism during the first 56 days of its development following fertilization or creation, excluding any time during which its development has been suspended, and includes any cell derived from such an organism that is used for the purpose of creating a human being. (hinxtongroup.org)
… "human clone" means an embryo that, as a result of the manipulation of human reproductive material or an in vitro embryo, contains a diploid set of chromosomes obtained from a single - living or deceased - human being, fetus, or embryo. (hinxtongroup.org)
The egg is artificially stimulated to divide and behave in a similar way to an embryo fertilised by sperm. (eurostemcell.org)
After many divisions in culture, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original donor who provided the adult cell - a genetic clone. (eurostemcell.org)
Hwang and colleagues report that the cells are chromosomally normal, self-renewing and "pluripotent" - meaning they have the ability to form the three major types of cells in the early embryo that give rise to all other cells in the body. (scienceblog.com)
This question had been asked by embryologists since 1886 ( Rauber, 1886 ), and Spemann ( Spemann, 1938 ) had demonstrated by an egg ligation experiment that the nuclei of an eight-cell frog embryo are developmentally totipotent. (biologists.com)
However, Briggs and King ( Briggs and King, 1957 ) had also found that the nucleus of an endoderm cell from a neurula embryo could no longer support normal development ( Fig. 2 ). (biologists.com)
But it is perhaps not auspicious to quote him for purposes of the scientific debates on human cloning, because Ramsey agreed with and supported the scientific myth of the "pre-embryo" 47 made famous by Jesuit Richard McCormick and frog embryologist Clifford Grobstein. (lifeissues.net)
Perhaps Ramsey would give other extraordinarily powerful arguments as to why human cloning is unethical, but he obviously would not be able to base it on his unscientific "pre-embryo" position. (lifeissues.net)
This process gets rid of unneeded cells and is particularly important for "sculpting" tissue and organ structure during development of the embryo (or larval metamorphosis in insects), but may occur at any time even in adult cells when a tissue needs to be remodeled. (agemed.org)
We can therefore disentangle the "life issue" of embryo-destruction from the "dignity issue" of baby manufacture, and enact a legislative ban on cloning and other degrading forms of baby-making, as recommended unanimously by the President's Council on Bioethics: Prohibit all attempts to conceive a child by any means other the union of egg and sperm, both obtained from adults. (bioedge.org)
Normally, the embryo comes into being through sexual conception, in which the female egg cell is fertilized by a male sperm cell. (actionlife.org)
Keith Henry Stockman Campbell studied embryo growth and cell differentiation during the twentieth and twenty-first centuries in the UK. (asu.edu)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
Last year, Hwang's team said it successfully cloned a human embryo from embryonic stem cells. (blogspot.com)
According to MBC, the scientist "maintains that Hwang's team fabricated data because in reality it failed to clone a somatic cell and instead used a frozen embryo from the hospital to make stem cells. (blogspot.com)
This union marks fertilization and the fertilized egg undergoes series of mitotic divisions to give rise to an embryo comprised of diploid cells. (biologyonline.com)

The egg is now viable and capable of producing an adult organism containing all necessary genetic information from just one parent. (wikipedia.org)
These cells genetically matched the donor organism from which they came. (wikipedia.org)
These cells are deemed to have a pluripotent potential because they have the ability to give rise to all of the tissues found in an adult organism. (wikipedia.org)
On the other hand, a chimera is defined as an organism in which cells from two or more different organisms have contributed. (frontiersin.org)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
At that point - and this is important to understand - there is no more cloning to be done since a new human organism now exists. (cbc-network.org)
Or to put it the other way around, cloning, not implantation, is what produces a new and distinct human organism. (cbc-network.org)
At the top of the list comes the zygote-a fertilized egg, which of course has the ability to divide and differentiate into all cell types in the body and create a new organism. (thefutureofthings.com)
The first three divisions of the zygote give birth to eight totipotent cells, each of which also has the ability to become an entire organism. (thefutureofthings.com)
A new organism is created by asexual reproduction using a duplicate of a single cell from the parent organism. (geminigenetics.com)
This is the most known form of cloning and involves creating a genetically identical replica of a whole organism. (geminigenetics.com)
The process of reproductive cloning involves the nucleus of a somatic (body) cell from a donor organism to be cloned being transferred into an egg cell whose nucleus (genetic material) has been removed. (geminigenetics.com)
XI - embryonic stem cells: embryonic cells that are capable of modifying the cells of any organism tissue. (hinxtongroup.org)
These stem cells are genetically matched to the donor organism, holding promise for studying genetic disease. (eurostemcell.org)
Cloning is the process of creating a genetically identical duplicate of an organism. (humansfuture.org)
The related concept of Longevity Determination , however, is the result of a species-specific genomic expression during early development that positions the somatic tissues of an organism to survive long after its reproductive period has been completed. (agemed.org)
Internal signals producing apoptosis depend on interactions of several proteins and may serve to protect the organism from cancer by killing cells that have pre-cancerous changes. (agemed.org)
This is cloning, a process in which the body cell that donated the replacement nucleus supplies the chromosomes of the new human organism. (actionlife.org)
Whether the new organism is produced by fertilization or by cloning, each new human organism is a distinct entity. (actionlife.org)
1 Reprogramming: Reprogramming is the process that adult somatic cells acquire the ability to differentiate into all cell types of an organism. (mynewsdesk.com)
A clone is an organism that is a genetic copy of an existing one. (who.int)
This is in contrast to asexual reproduction where an organism reproduces without involving gametes and the resulting offspring is a clone of the parent. (biologyonline.com)

The blastocyst stage is developed by the egg to help create embryonic stem cells from the inner cell mass of the blastocyst. (wikipedia.org)
A blastocyst (cloned or not), because it lacks any trace of a nervous system, has no capacity for suffering or conscious experience in any form - the special properties that, in our view, spell the difference between biological tissue and a human life worthy of respect and rights. (wikiquote.org)
To produce Dolly, the cloned blastocyst was transferred into the womb of a recipient ewe, where it developed and when born quickly became the world's most famous lamb. (eurostemcell.org)
Instead, embryonic stem cells are isolated from the cloned blastocyst. (eurostemcell.org)
Stem cells were then derived from the inner cell mass of the blastocyst. (scienceblog.com)
The Korean researchers who performed this stem cell research improved upon their protocols that yielded the first embryonic stem cell line from a cloned human blastocyst. (scienceblog.com)

In 1996, Dolly the sheep became famous for being the first successful case of the reproductive cloning of a mammal. (wikipedia.org)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
The cloning of 'Dolly The Sheep' in 1996 by the Rosalind Institute in Scotland, UK, is the most recognised example of reproductive cloning. (geminigenetics.com)
Scientists plan to use somatic cell nuclear transfer for the first human clone, which is the same technique that was used to create Dolly the sheep. (humansfuture.org)
This is essentially a variation on a process that was used some years ago to create a cloned sheep named Dolly. (intlstemcell.com)
I am talking about human stem cells derived from a process called, "Parthenogenesis", developed and first announced in 2007 by a company called International Stem Cell Corporation, whose discoveries were first published in the peer reviewed journal, Cloning and Stem Cells, edited by the scientist who first created "Dolly", the first cloned animal. (intlstemcell.com)
is a British developmental biologist who was the first to use nuclear transfer of differentiated adult cells to generate a mammalian clone, a Finn Dorset sheep named Dolly, born in 1996. (mathisfunforum.com)
Campbell and his team also cloned a sheep from adult cells in 1996, which they named Dolly. (asu.edu)

Somatic cell nuclear transplantation has become a focus of study in stem cell research. (wikipedia.org)
It is the policy of Washington state that research involving the derivation and use of human embryonic stem cells, human embryonic germ cells, and human adult stem cells from any source, including somatic cell nuclear transplantation , is permitted upon full consideration of the ethical and medical implications of this research. (cbc-network.org)
As a brand new graduate student starting in October 1956, my supervisor Michail Fischberg, a lecturer in the department of Zoology at Oxford, suggested that I should try to make somatic cell nuclear transplantation work in the South African frog Xenopus laevis . (biologists.com)
Sir John Bertrand Gurdon further developed nuclear transplantation, the technique used to clone organisms and to create stem cells, while working in Britain in the second half of the twentieth century. (asu.edu)

Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
In the ongoing debate about cloning human embryos for research, and about destroying them in order to harvest their stem cells, it is important to keep some basic facts in mind. (actionlife.org)

By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
The disease specific stem cell lines could then be studied in order to better understand the condition. (wikipedia.org)
In the United States, scientists at the Harvard Stem Cell Institute, the University of California San Francisco, the Oregon Health & Science University, Stemagen (La Jolla, CA) and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce embryonic stem cells. (wikipedia.org)
Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
A controversial scheme to extend the practise of ' egg sharing' has been approved by the UK's Human Fertilisation and Embryology Authority (HFEA) to provide greater numbers of eggs for embryonic stem cell (ES cell) research. (progress.org.uk)
The cost will be met by the North-East England Stem Cell Institute (Nesci). (progress.org.uk)
Peter Braude, Professor of Obstetrics and Gynaecology at Kings College London, who has also been granted HFEA licences for embryonic stem cell research in the past commented, 'This is a difficult situation because there is a strong need for eggs for research. (progress.org.uk)
Victoria and New South Wales have put aside their competitive interstate rivalry to collaborate on a stem cell research project, as announced by Innovation Minister Gavin Jennings and NSW Minister for Science and Medical Research, Verity Firth, today. (medicalxpress.com)
Dr Paul Verma, Program Leader of MIMR's Stem Cell Biology Program and the Chief Investigator of the Victorian project team, will create the iPS cells for this project. (medicalxpress.com)
Professor Tuch's team will also use their proficiencies in characterising human embryonic stem cell lines and bioinformatics. (medicalxpress.com)
Mr Jennings said changes to the Victorian Infertility Treatment Act and the NSW Human Cloning and Other Prohibited Practices Act have opened up new opportunities for stem cell research in Australia. (medicalxpress.com)
The bill purports to promote stem-cell research, while outlawing the cloning of a human being. (cbc-network.org)
While stem-cell research holds enormous potential for treating or even curing some diseases, the cloning of a human being is morally and ethically unacceptable…Any attempt to clone a human being is in direct conflict with the public policies of this state. (cbc-network.org)
Snuppy was cast under suspicion following revelations that the Korean scientist had fabricated his stem cell research. (blogspot.com)
It brightens the prospects that his team retains the source technologies for stem cell research," Park said. (blogspot.com)
Only two of Hwang's 11 claimed stem-cell lines existed when he submitted his 2005 paper to the journal, Science, the university said on Dec. 23. (blogspot.com)
It's given name is the "Human Cloning Ban and Stem Cell Research Protection Act of 2003," the stated purpose of which, supposedly, is to "prohibit human cloning and to protect important areas of medical research, including stem cell research. (lifeissues.net)
While regarded by many top scientists as the Holy Grail of medicine, others consider embryonic stem-cell research sacrilegious. (thefutureofthings.com)
Recent advances in the field of stem-cell research are giving hope to millions. (thefutureofthings.com)
Avant-garde approaches to stem-cell therapy may be the first stepping-stones to a bright new future of stem-cell medicine and are emerging in leading laboratories worldwide. (thefutureofthings.com)
The term stem cell can be defined by two very important qualities: the cell has the ability to self-renew and, in a more general sense, the cell has not completed differentiation into its final state. (thefutureofthings.com)
A particular field encouraged by the foundation is stem-cell research, with the great hope that it will result in the ability to get cells to differentiate into neurons and support cells to bridge the gap of a spinal cord injury. (thefutureofthings.com)
A linkurl:report;http://stemcells.alphamedpress.org/cgi/reprint/2007-0252v1.pdf published online today that researchers have cloned human embryos is not that much of an advance, according to one stem cell expert, Douglas Melton, at Harvard University. (the-scientist.com)
It would be hard to call this a major advance," Douglas Melton, a stem cell researcher at Harvard University, told The Scientist in an Email. (the-scientist.com)
What is cloning, and what does it have to do with stem cell research? (eurostemcell.org)
This form of cloning is unrelated to stem cell research. (eurostemcell.org)
Scientists have isolated the first human embryonic stem cell lines specifically tailored to match the nuclear DNA of patients, both males and females of various ages, suffering from disease or spinal cord injury. (scienceblog.com)
Each of the 11 new human embryonic stem cell lines was created by transferring the nuclear genetic material from a non-reproductive cell of a patient into a donated egg, or "oocyte," whose nucleus had been removed. (scienceblog.com)
The stem cell lines produced from patients with disease will likely display characteristics of the disease, so they will probably not be appropriate for direct use in treating patients. (scienceblog.com)
In a Science "Policy Forum" related to the team's latest findings, David Magnus and Mildred Cho from Stanford University in Palo Alto, CA discuss international oversight and ethical issues in oocyte donation, including the need to promote realistic expectations of the outcomes of stem cell research. (scienceblog.com)
From the 31 nuclear-transfer blastocysts, the scientists derived 11 stem cell lines. (scienceblog.com)
The researchers generated these stem cell lines ten times more efficiently than in their 2004 Science study, using improved laboratory methods. (scienceblog.com)
The fall from grace of stem cell scientist Hwang Woo-suk would be as spectacular as his meteoric ascent. (koreatimes.co.kr)
The neo-conservative administration of George W Bush ― an administration with a strongly Christian support base to appease ― banned stem cell research. (koreatimes.co.kr)
As a result, International Stem Cell will benefit from the development of either technology, but it is important that the public and the scientific community be fully aware of all alternatives in the field of regenerative medicine, not just the ones that capture public imagination at any particular time. (intlstemcell.com)
Protocols are approved by Independent Review Boards (IRBs) to protect the safety of donors and by an independent Stem Cell Research Oversight (SCRO) committee to insure compliance with state laws and research ethics, regulations established by the U.S. Food and Drug Administration (FDA) and the U.S. Department of Health and Human Services (HHS) Office for Human Research Protections, in addition to state-level requirements. (intlstemcell.com)
In fact, by some estimates, as few as 100 parthenogenetic stem cell lines could provide immune-matched cells to over 50 percent of the world's population, and could accelerate disease therapies and treatments for severe chronic conditions, including diabetes, spinal cord injuries, liver diseases, blinding diseases such as macular degeneration, and neural diseases such as Parkinson's and Alzheimer's. (intlstemcell.com)
Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
Not only might stem cell technology obviate the need for cadaver donation - it could also resolve the problem of rejection. (spiked-online.com)
Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
There are major obstacles to overcome before a stem cell line is liable to reach clinical trials. (spiked-online.com)
In November 22nd, 2016, The Korean Intellectual Property Office has officially certified Sooam's Patent Application on Embryonic Stem Cell Line and its method of generation. (sooam.com)
The Korea Centers for Disease Control and Prevention has registered "Sooam-hES-1" human stem cell line to the National Stem Cell Bank of National Centre for Stem Cell and Regenerative Medicine. (sooam.com)
Sooam-hES-1" stem cell line is noted for its molecular and developmental capacities as an embryonic stem cell. (sooam.com)
Update 05/23/2013: OHSU releases statement on questions about photos in stem cell paper. (ohsu.edu)
It is believed that stem cell therapies hold the promise of replacing cells damaged through injury or illness. (ohsu.edu)
Diseases or conditions that might be treated through stem cell therapy include Parkinson's disease, multiple sclerosis, cardiac disease and spinal cord injuries. (ohsu.edu)
While there is much work to be done in developing safe and effective stem cell treatments, we believe this is a significant step forward in developing the cells that could be used in regenerative medicine. (ohsu.edu)
This is a remarkable accomplishment by the Mitalipov lab that will fuel the development of stem cell therapies to combat several diseases and conditions for which there are currently no treatments or cures," said Dr. Dan Dorsa, Ph.D. , OHSU Vice President for Research. (ohsu.edu)
And the MSM (mainstream media), who remain desperately committed to push embryonic stem cell research despite its failures, dangers, and immoral foundations, may soon be reeling at the fall of one of their heroes. (blogspot.com)
hero" is the right word for how Hwang Woo-suk is revered by the media -- and by a large section of the Korean public who have bought into the false promises of embryonic stem cell and cloning researchers. (blogspot.com)
embryonic stem cell research has not had a good year. (blogspot.com)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)

That is what New Jersey legislators did when they passed and then Governor James McGreevey signed S-1909 last year, a law that was sold to the public as outlawing human cloning but which actually permits the creation of cloned human life, and its implantation and gestation up to and including the very moment prior to the emergence of the cloned baby from the birth canal. (cbc-network.org)

Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
Dec. 29, 2005 -- South Korean laboratories used by scientist Hwang Woo Suk no longer have any stem cells created from patients' tissues, the result of the researcher's landmark May 2005 paper, the Seoul National University said. (blogspot.com)
In addition to their ability to supply cells at the turnover rate of their respective tissues, they can be stimulated to repair injured tissue caused by liver damage, skin abrasions and blood loss. (thefutureofthings.com)
The ability of our body to regenerate some of its tissues is largely owed to the reserves of adult stem cells. (thefutureofthings.com)
Numerous biological components, including genes, cells, tissues, and even complete creatures like sheep, have been cloned by researchers, and now cat, dog and equine cloning is widely and reliably available via international companies such as our partner, ViaGen Pets & Equine. (geminigenetics.com)
Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
By this technique, the team established primary cultured cells using tissues collected from postmortem Oryx dammah, a species extinct in the wild, and succeeded in activating embryonic genes from their cell nuclei. (mynewsdesk.com)

The technique consists of taking an denucleated oocyte (egg cell) and implanting a donor nucleus from a somatic (body) cell. (wikipedia.org)
The first being a female gamete, known as the ovum (egg/oocyte). (wikipedia.org)
In the case of asexually creating a human, the biotechnologist removes the nucleus from a mature human egg (an oocyte). (cbc-network.org)
Oocyte donors and patients who donated non-reproductive cells were all unpaid volunteers. (scienceblog.com)
The single cell line generated in the 2004 Science paper resulted from nuclear transfer in which the oocyte and non-reproductive ("somatic") cell came from the same healthy female. (scienceblog.com)
7. "[footnote 16]: The cloning procedure supplies the oocyte with a complete set of chromosomes, all of which are contained in the nucleus which is transferred into the denucleated oocyte. (lifeissues.net)

In January 2018, a team of scientists in Shanghai announced the successful cloning of two female crab-eating macaques (named Zhong Zhong and Hua Hua) from foetal nuclei. (wikipedia.org)
Treatment at the Newcastle NHS Fertility Centre, which will offer the scheme, usually costs £3,500, if a woman agrees to surrender half of her derived eggs to scientists the fee will be halved. (progress.org.uk)
The eggs will then be used by scientists from Newcastle and Durham Universities to create embryos from which they will attempt to derive stem cells . (progress.org.uk)
Until now scientists have been restricted to using 'left-over' eggs from IVF treatment, these are usually poor quality and are already older than those that will be donated through the new scheme. (progress.org.uk)
Discussing the planned public consultation Angela McNab, chief executive of the HFEA, said, 'We know there are a wide variety of views on the subject of donating eggs for research and we anticipate a strong response to the consultation from professional groups, scientists, clinicians and patients as well as the public. (progress.org.uk)
The work also moves scientists one step closer to the goal of transplanting healthy cells into humans to replace cells damaged by diseases such as Parkinson's and diabetes. (scienceblog.com)
Religious groups believe that the raw material from which stem cells are sourced are themselves forms of human life, and by creating little chunks of humans in Petri dishes, scientists are, critics believe, playing God. (koreatimes.co.kr)
The exact process of differentiation is not yet understood and although embryonic stem cells can, in principle, provide for all human tissue, scientists are some way from controlling the process. (spiked-online.com)
It might be expected that the richest nation on Earth would encourage its top scientists to pursue this work with vigor rather than limiting funding opportunities, creating legal barriers and fencing off any newly developed cell lines. (spiked-online.com)
Gurdon's research built on the work of Thomas King and Robert Briggs in the United States, who in 1952 published findings that indicated that scientists could take a nucleus from an early embryonic cell and successfully transfer it into an unfertilized and enucleated egg cell. (asu.edu)
1. Cloning is an umbrella term traditionally used by scientists to describe different processes for duplicating biological material. (who.int)
Scientists at Oregon Health & Science University and the Oregon National Primate Research Center (ONPRC) have successfully reprogrammed human skin cells to become embryonic stem cells capable of transforming into any other cell type in the body. (ohsu.edu)
The advance, if confirmed, might provide a new source of eggs for treating infertility, though scientists say it is far too early to tell if the work holds such promise…The new research, by a team led by the biologist Jonathan L. Tilly, depends on a special protein found to mark the surface of reproductive cells like eggs and sperm. (cbc-network.org)

After being inserted into the egg, the somatic cell nucleus is reprogrammed by its host egg cell. (wikipedia.org)

The Ethical Debate Concerning Cloning In the year that has elapsed since the announcement of Dolly's birth, there has been much discussion of the ethical implications of cloning humans. (bartleby.com)
However, the idea of cloning humans is a highly charged topic. (bartleby.com)
In rodents, and even in some preliminary trials in humans, human embryonic stem cells have been shown to bridge gaps in spinal cord injuries , allowing restoration of motor functions. (thefutureofthings.com)
Humans and other mammals may produce natural clones, commonly referred to as identical twins. (geminigenetics.com)
In most countries, it is illegal to attempt reproductive cloning in humans. (eurostemcell.org)

Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)
2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)

A Korean television station whose investigative report was the nail in the coffin that prompted human cloning scientist Hwang Woo-suk to admit he lied about egg donations his researchers made says he may have lied about the results of his research as well. (blogspot.com)

Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)

In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)
2 Cloning technology: Development of reconstructed embryos produced by transplanting cell nuclei into pre-fertilized eggs is induced by activation stimulation, and then the reconstructed embryos are to be implanted in oocytes to generate cloned animals that share identical genomes as the donor cells. (mynewsdesk.com)

Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)

These adult stem cells are considered multipotent, having the ability to differentiate into different cell types, albeit with a more limited repertoire than embryonic stem cells. (thefutureofthings.com)
Embryonic stem cells are derived from early embryos and have the ability to differentiate into any cell type. (spiked-online.com)

In their new paper, Science author Woo Suk Hwang from Seoul National University in Seoul, Korea and colleagues replaced the nuclei from donated oocytes with nuclei from skin cells from male and female patients, ranging in age from 2 to 56, who had spinal cord injuries, juvenile diabetes and the genetic disease "congenital hypogamma-globulinemia. (scienceblog.com)
Even advanced donor cells from the endoderm of Xenopus tadpoles have nuclei that can sometimes yield normal individuals after nuclear transfer [data taken from Briggs and King ( Briggs and King, 1957 ) for Rana and from Gurdon ( Gurdon, 1962 ) for Xenopus ]. (biologists.com)
This allows induction of reprogramming of various animal cell nuclei, which was difficult with conventional cloning methods. (mynewsdesk.com)
The research team succeeded in inducing the activation of embryonic genes*4, which is an essential reprogramming phenomenon, with various animal somatic nuclei in mouse embryos arrested at the early stage, which has not been used in conventional cloning techniques. (mynewsdesk.com)

Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. (wikiquote.org)
This technique was developed by modifying the conventional cloning technology*2 and is the world's first successful induction of gene activation*3, which is specifically expressed in early embryos from genomes of Oryx dammah, now extinct in the wild. (mynewsdesk.com)
The new cloning technology developed in this research will make it possible to analyze the functions of the endangered or extinct animal genomes. (mynewsdesk.com)

The second way to reproduce is a strictly human invention - known as "asexual" reproduction - or more commonly, cloning. (cbc-network.org)
Asexual reproduction is a natural method used by certain plants, bacteria, and single-celled creatures to create genetically identical offspring, i.e. clones. (geminigenetics.com)

The genetic material of the donor egg cell is removed and discarded, leaving it 'deprogrammed. (wikipedia.org)
Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
Lytic cycle - the replication process in viruses in which the virus's genetic material uses the copying machinery of the host cell to make new viruses. (edrawmind.com)
Replication - the fundamental process of all cells, in which the genetic material is copied before the cell reproduces. (edrawmind.com)
The DNA within the skin sample is cultured and inserted into a donor egg cell whose nucleus (genetic material) has been removed. (geminigenetics.com)
From the 185 donated oocytes, endowed with the genetic material from a different person (or in one case, the same person), the researchers report development of 31 hollow balls of cells called "human nuclear-transfer blastocysts. (scienceblog.com)
It involves transplanting the nucleus of one cell, containing an individual's DNA, into an egg cell that has had its genetic material removed. (ohsu.edu)
Furthermore, because these reprogrammed cells can be generated with nuclear genetic material from a patient, there is no concern of transplant rejection," explained Dr. Mitalipov. (ohsu.edu)
Metaphase is a stage in the cell's natural division process (meiosis) when genetic material aligns in the middle of the cell before the cell divides. (ohsu.edu)
The sperm cell upon finding the egg cell will penetrate it so that their genetic material could combine as one full set (diploid). (biologyonline.com)

Here, we transfected the LMNB1 gene into the telomerase reverse transcriptase-immortalized benign prostatic epithelial cell line, EP156T to generate a LMNB1-overexpressing EP156T (LMN-EP156T) cell line with increased cellular proliferation. (bvsalud.org)
Researchers at Massachusetts General Hospital say they have extracted stem cells from human ovaries and made them generate egg cells. (cbc-network.org)
Using a cell-sorting machine that can separate out the marked cells, the team obtained reproductive cells from mouse ovaries and showed that the cells would generate viable egg cells that could be fertilized and produce embryos. (cbc-network.org)

Moreover, most early-stage embryos that are produced naturally (that is, through the union of egg and sperm resulting from sexual intercourse) fail to implant and are therefore wasted or destroyed. (wikiquote.org)
The first is that great old standard, "sexual" reproduction, in which sperm meets egg. (cbc-network.org)
2. A somatic cell (a non-sperm, non-egg cell), which contains DNA, is then taken from the person to be cloned. (humansfuture.org)
Should there be a 'dead donor rule' for sperm donation? (bioedge.org)
In sexual reproduction the new individual gets half of its chromosomes from the nucleus of the sperm cell and half from the nucleus of the egg cell. (actionlife.org)
One of these techniques stages the encounter of sperm with egg in a laboratory dish rather than in a woman's body. (actionlife.org)
egg and sperm). (biotechfront.com)
On the other hand, in germline gene therapy , the egg and sperm cells of the parents are altered to be passed on to their offspring. (biotechfront.com)
The egg cells, when injected into mice, generated follicles, the ovarian structure in which eggs are formed, as well as mature eggs, some of which had a single set of chromosomes, a signature of eggs and sperm. (cbc-network.org)
Sexual reproduction is a mode of reproduction involving the fusion of haploid female gamete (egg cell) and haploid male gamete ( sperm cell ). (biologyonline.com)
In males, the mature gamete is a sperm cell (also called a spermatozoon ). (biologyonline.com)

A type of cloning that occurs naturally is when identical twins are born ("What Is Cloning? (bartleby.com)
There are also naturally occurring clones among animal populations. (geminigenetics.com)
As well as their distinctive white markings and long curved horns, these cattle are special because they are now considered a herd of naturally occurring clones. (geminigenetics.com)

Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
The genetically modified egg now has 46 chromosomes, the full human compliment. (cbc-network.org)

In 1995, Campbell and his scientific team used cells grown and differentiated in a laboratory to clone sheep for the first time. (asu.edu)

If there are intact cells in this tissue they have been 'stored' frozen. (wikiquote.org)
and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
Current screening of potential new drugs is done using cell lines derived from animals or 'abnormal' human tissue such as tumor cells. (spiked-online.com)
Various labs are working together to try and culture mammoth tissue and obtain a viable cell. (sooam.com)

In this procedure, the nucleus of an egg cell is removed and replaced by the nucleus of a cell from another adult. (eurostemcell.org)
Currently, the procedure for isolating non-reproductive cells for the nuclear transfer method involves animal enzymes and serum. (scienceblog.com)
No donor is every subjected to any additional physical risk beyond what she has already agreed to as part of the IVF procedure in which she elected to participate. (intlstemcell.com)

The stem cells could be studied in the laboratory to help researchers understand what goes wrong in diseases like these. (eurostemcell.org)
Before patient-specific stem cells can potentially be used in the clinic, a variety of issues must be addressed, the researchers emphasized. (scienceblog.com)
In addition, researchers must develop methods to efficiently direct the differentiation of embryonic stem cells to specific stable cell types. (scienceblog.com)
Through moving findings between monkey cells and human cells, the researchers were able to develop a successful method. (ohsu.edu)
Paul Root Wolpe of the Center for Bioethics at the University of Pennsylvania points out that Hwang did not resign because he used eggs from his researchers but because he lied about it and brought shame "in a country where public shame is so powerful. (blogspot.com)

After the somatic cell transfers, the cytoplasmic factors affect the nucleus to become a zygote. (wikipedia.org)
It was clear that a definitive experiment required the replacement of a zygote nucleus by a somatic cell nucleus, asking whether the somatic nucleus could functionally replace the zygote nucleus by eliciting normal development of the enucleated recipient egg ( Fig. 1 )? (biologists.com)
Every human being begins as a single-cell zygote, grows through the embryonic stage, then the fetal stage, is born and develops through infancy, through childhood, and through adulthood, until death. (actionlife.org)
The egg is then stimulated by an electrical charge, creating a living human zygote. (actionlife.org)
Syngamy refers to the fusion of haploid sex cells resulting in the formation of a diploid zygote. (biologyonline.com)

With this, Hwang demonstrated his team's technical prowess in cloning. (blogspot.com)
The Mitalipov team's success in reprogramming human skin cells came through a series of studies in both human and monkey cells. (ohsu.edu)

The surrogate mum carries the cloned pet for the gestation period and once ready, gives birth to the clone who will be an identical genetic twin to the original pet whose skin sample was used to make the nucleus of the donor egg cell. (geminigenetics.com)
The three parties will work together to retrieve and research mammoth samples for the purpose of understanding their genomics and possibly cloning a live mammoth from a surrogate elephant effectively restoring the mammoth from extinction. (sooam.com)

The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)

Should the use of nuclear transfer ever become widespread there will be an urgent need for human egg donors and it is unlikely that the willing population will be sufficient to meet demand. (spiked-online.com)
Previous unsuccessful attempts by several labs showed that human egg cells appear to be more fragile than eggs from other species. (ohsu.edu)

The process of somatic cell nuclear transfer involves two different cells. (wikipedia.org)
The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
This process allows people to clone living things of any sort. (bartleby.com)
In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
If it is to be brought to birth, the process is usually called "reproductive cloning. (cbc-network.org)
Pet cloning is the process where a genetically identical twin is created of your original animal companion. (geminigenetics.com)
When the cloning process is used in this way, to produce a living duplicate of an existing animal, it is commonly called reproductive cloning. (eurostemcell.org)
For example, stem cells could be generated using the nuclear transfer process described above, with the donor adult cell coming from a patient with diabetes or Alzheimer's. (eurostemcell.org)
What is not generally known is that the IVF process can often result in the creation of far more unfertilized eggs than will ever be needed for fertility purposes. (intlstemcell.com)
In fact, all egg donors voluntarily participate through a very transparent, peer-reviewed, and medically supervised process. (intlstemcell.com)
His embryological work consisted of experiments in understanding the process of generation, and led him to adopt the model of preformationism called ovism (the idea that the new individual exists within the maternal egg prior to conception). (asu.edu)
The close proximity of the laboratory with the expedition sites which will hopefully accelerate the cell obtaining process that is the basis for cloning. (sooam.com)
The key to this success was finding a way to prompt egg cells to stay in a state called "metaphase" during the nuclear transfer process. (ohsu.edu)
The research team found that chemically maintaining metaphase throughout the transfer process prevented the process from stalling and allowed the cells to develop and produce stem cells. (ohsu.edu)
While ethical issues about (egg) donation should be debated and the process regulated, the scientific conclusions of Dr. Hwang's research remain intact," Schatten said in a statement. (blogspot.com)
In brief, gametogenesis is a biological process that incorporates cell divisions, particularly mitosis and meiosis , to produce gametes (sex cells). (biologyonline.com)

However, donor organs are in absolute shortage, and sadly, most patients die while waiting for a donor organ. (frontiersin.org)
Presently, in the United States, another person is added to an organ transplant list every 10 min, 17 people die each day while waiting for donor organs, and approximately 105,800 patients are waitlisted for an organ transplant according to the health resources and services administration (HRSA). (frontiersin.org)
The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)

These are then fused by inserting the somatic cell into the 'empty' ovum. (wikipedia.org)
The ovum, now containing the somatic cell's nucleus, is stimulated with a shock and will begin to divide. (wikipedia.org)
In females, the mature gamete is an egg cell (also called an ovum ). (biologyonline.com)

General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)

Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)

In particular, genomic reprogramming resets the epigenetic information accumulated in the adult somatic cells, and the cells revert back to an early embryonic state. (mynewsdesk.com)

Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)
Instead, it may depend on the host and donor molecular similarities or distinctions critical for the organogenesis program. (frontiersin.org)
Molecular cloning refers to the production of multiple copies of a DNA fragment or gene. (geminigenetics.com)
In summary, LMNB1 may play a role in the early steps of PC progression, and additional molecular alterations may be needed to confer full malignancy potential to initiated cells. (bvsalud.org)

These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
Claims that you could clone individual treatments of human beings to treat common diseases like diabetes, suggests you need a huge supply of human eggs. (wikiquote.org)
The recovered stem cells can then be used in the treatment of diseases and to aid the recovery of injuries. (geminigenetics.com)
Moreover, they can be used independently to research how, for example, diseases attack cells. (koreatimes.co.kr)

As with the mice, the team was able to retrieve reproductive cells that produced immature egg cells when grown in the laboratory. (cbc-network.org)

A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patient's particular disease. (wikipedia.org)

A Korean institute Wednesday, Dec. 27, said that its DNA tests proved Prof. Hwang Woo-suk at Seoul National University (SNU) had successfully cloned a dog. (blogspot.com)
Several years of monkey studies that utilize somatic cell nuclear transfer have never successfully produced monkey clones. (ohsu.edu)
However, Korean television station MBC has conducted interviews with an unnamed member of Hwang's research team who says the cells were never cloned successfully. (blogspot.com)

from nationalreview.com Let's call it "stealth human-cloning legalization. (cbc-network.org)
article: Now, he's done it again by signing up as a co-sponsor (along with Senators Orin Hatch and Dianne Feinstein) of what could be called the Human Cloning Legalization and Legitimization Act of 2003 (S. 303) . (lifeissues.net)
The cloning "bans" being supported in his article could likewise be called "the Human Cloning Legalization and Legitimazation Acts of 2003" (e.g. (lifeissues.net)

These cell lines will enable the study of human disease in cells in the laboratory. (scienceblog.com)
In laboratory culture, these cell lines displayed signs of immunological compatibility with the patients' cells, Science authors reported. (scienceblog.com)
Other improvements over the last paper include the reduced use of animal products in laboratory procedures and better evidence that the cell lines matched the patients' cells and did not have a parthenogenetic origin, where unfertilized eggs can divide on their own. (scienceblog.com)
Dr Thomas Okarma, who heads up the Geron Corporation, claims to have heart cells that 'beat' in laboratory dishes developed from stem cells (2). (spiked-online.com)

Although the simple use of the word 'clone' may have negative connotations, many people have resigned themselves to the idea of cloning cows that produce more milk or using a cloned mouse for use in controlled experimentation. (bartleby.com)
Hwang's early work was with pigs and cows, though his cloning experiments in this field, while gaining him some visibility within Korea, were not backed up by internationally credible data. (koreatimes.co.kr)

Somatic cell nuclear transfer is a technique for cloning in which the nucleus of a somatic cell is transferred to the cytoplasm of an enucleated egg. (wikipedia.org)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
A thorough examination of the stem cells derived through this technique demonstrated their ability to convert just like normal embryonic stem cells, into several different cell types, including nerve cells, liver cells and heart cells. (ohsu.edu)

Both methods need to be investigated further so we can determine which will produce the most robust cell lines," Dr Verma said. (medicalxpress.com)
The present report gives an overview of the terms and methods used in cloning and summarizes the debates in the General Assembly. (who.int)
As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)
Detailed descriptions of methods used in animal cloning and biotechnology are provided in the report Animal Biotechnology: Science-Based Concerns (NRC, 2002). (nationalacademies.org)
In addition, the committee was charged with evaluating methods to detect potential, unintended, adverse health effects of foods derived from cloned animals. (nationalacademies.org)
Alternatively, research using eggs may point the way to methods which mimic their properties using other human cells and chemical agents. (spiked-online.com)
Therefore, known reprogramming methods stalled before stem cells were produced. (ohsu.edu)

Reprogramming of human somatic cells to pluripotency is an enormously significant achievement, one that boosters of medical progress and defenders of human dignity can celebrate without qualification… The ethical and political benefits may be equally great. (bioedge.org)
Various cloned animals have been created with the reprogramming technology that restores cells to the undifferentiated state by transplanting differentiated adult somatic cells into oocytes, and it has been also expected to revive endangered or extinct species. (mynewsdesk.com)
Here, we summarize current reprogramming methodologies with a focus on the production of transgene-free or genetically unmanipulated iPSCs and highlight important technical details that ultimately may influence the biological properties of pluripotent stem cells. (biomedcentral.com)
To circumvent these obstacles, considerable effort has been invested in attempting to derive ESC-like cells by reprogramming somatic cells to an embryonic state. (biomedcentral.com)
Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)

Anatomy59

Organisms22

Diseases4

Chemicals and Drugs58

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes76

Disciplines and Occupations1

Technology, Industry, Agriculture6

Information Science5

Named Groups4

Health Care2

Therapeutic19

SCNT38

Embryo28

Organism22

Blastocyst6

Dolly9

Nuclear transplantation4

Embryos for research2

Stem cell55

Transplantation3

Creation of cloned human1

Tissues7

Oocyte6

Scientists13

Nucleus is reprogrammed1

Humans5

Reproductive cloning of huma5

Scientist Hwang Woo1

20231

Identical genomes2

Regenerative medicine1

Ability to differentiate2

Nuclei4

Genomes3

Asexual2

Genetic material11

Generate3

Sperm11

Naturally3

Chromosomes2

Sheep1

Tissue4

Procedure3

Researchers5

Zygote5

Team's2

Surrogate2

Cytoplasmic factors1

Human egg2

Process17

Organs3

Ovum3

Endorsed in 19971

Create embryonic stem1

Early embryonic1

Molecular4

Diseases4

Immature egg cells1

Genetically matched1

Successfully3

Legalization3

Laboratory4

Cows2

Technique3

Methods7

Reprogramming5