Donor cell stem cells and cloning. Medical search. Frequent questions

In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
this approach has been championed as an answer to the many issues concerning embryonic stem cells (ESCs) and the destruction of viable embryos for medical use, though questions remain on how homologous the two cell types truly are. (wikipedia.org)
Controversy surrounds human ESC work due to the destruction of viable human embryos, leading scientists to seek alternative methods of obtaining pluripotent stem cells, SCNT is one such method. (wikipedia.org)
Embryonic stem cells come from human embryos that are three to five days old. (healthline.com)
A linkurl:report;http://stemcells.alphamedpress.org/cgi/reprint/2007-0252v1.pdf published online today that researchers have cloned human embryos is not that much of an advance, according to one stem cell expert, Douglas Melton, at Harvard University. (the-scientist.com)
Several of the reconstructed oocytes developed as normal embryos, although only one of the blastocysts contained donor DNA or mitochondrial DNA. (the-scientist.com)
Researchers reported in Nature on November 22, 2007, that they successfully isolated 2 embryonic stem cell lines from cloned embryos made using cells from the skin of an adult rhesus macaque. (nih.gov)
The researcher claimed he had created stem cell lines from cloned human embryos. (asianews.it)
Embryonic stem cells can be derived legally from surplus embryos donated following IVF treatment. (eurostemcell.org)
According to the act, surplus embryos donated following IVF treatment can be used for research purposes, subject to the informed consent of the donor(s). (eurostemcell.org)
2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
Similar to embryonic stem cells (ESCs), hpSCs are pluripotent cells that can be reproduced into any type of cell within the human body without using or destroying the viable human embryos. (pharmaceutical-technology.com)
Embryonic stem cells are derived from early embryos and have the ability to differentiate into any cell type. (spiked-online.com)
One aspect to this project will be to source oocytes, or immature egg cells to generate SCNT embryos from which embryonic stem cells are harvested. (medicalxpress.com)
The recent desperation to clone human embryos may be seriously undermining accepted ethical principles of medical research, with potentially profound wider consequences. (lifeissues.net)
Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
The eggs will then be used by scientists from Newcastle and Durham Universities to create embryos from which they will attempt to derive stem cells . (progress.org.uk)
Citizens disagree about whether we should destroy human embryos for their stem cells-and if so, which embryos, with whose money, under what regulatory guidelines. (eppc.org)
The holy grail of regenerative medicine-whatever one's ethical beliefs about destroying embryos-is to "reprogram" regular cells from one's own body so that individuals can be the source of their own rejection-proof therapies. (eppc.org)
That is to say, we risk turning developed cells into developing embryos, and thus risk engaging in the very activities of embryo destruction and human cloning that we seek to avoid. (eppc.org)
Far more controversial-and for good reason-are stem cells derived from destroyed human embryos. (eppc.org)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
The use of embryonic stem cells has been a source of considerable controversy due to its sacrifice of human embryos in the blastocyst stage, which some people view as the destruction of human life . (citizendium.org)
Human embryos fertilized in the ordinary manner and harvested in the blastocyst stage have been used as an extensive source of stem cells for research purposes, and have been shown to possess therapeutic value in laboratory animals. (citizendium.org)
The most infamous study of embryonic stem cells asserted that cloned human embryos had been created via somatic cell nuclear transfer, and stem cells had been generated from these embryos. (citizendium.org)
Ethical objections to the use of human embryonic stem cells revolve around the destruction of human embryos in the blastocyst stage to obtain the stem cells. (citizendium.org)
It also produces mosaic embryos where some cells get fixed, others don't. (cosmosmagazine.com)
Mitalipov also carries the distinction of being the first to crack the long-standing problem of cloning human embryos and deriving embryonic stem cells. (cosmosmagazine.com)
Out of 58 embryos, 42 showed the normal gene in every cell. (cosmosmagazine.com)

Embryonic stem cells are known as pluripotent stem cells. (healthline.com)
Scientists have recently discovered how to turn adult stem cells into pluripotent stem cells. (healthline.com)
These new types of cells are called induced pluripotent stem cells (iPSCs). (healthline.com)
The goal of CIRM Grant ID1-06557 is to generate high quality induced pluripotent stem cells (iPSC) from blood and skin samples from 3000 donors, many of whom suffer from untreatable medical conditions, and place them in a Repository accessible to scientists around the world. (ca.gov)
Unlike induced pluripotent stem cells (iPSs), hPSCs do not manipulate the gene expression process back to a less differentiated stage, thereby avoiding the regulatory, ethical and safety obstacles. (pharmaceutical-technology.com)
Although the work has progressed very smoothly, one thing has continued to nag at her: is it actually safe to transplant induced pluripotent stem cells (iPSCs), or are these cells potentially dangerous to patients? (embs.org)
Reprogramming skin cells into pluripotent stem cells is a pretty dramatic event, and there had been several papers published suggesting that reprogramming could cause harmful mutations. (embs.org)
Scientists from the Monash Institute of Medical Research (MIMR) and colleagues from New South Wales will compare two different methods of creating patient-specific stem cells: somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPS). (medicalxpress.com)
As an alternative approach, MSCs derived from induced pluripotent stem cells (iPSCs), which may lead to further standardized-cell preparations. (biomedcentral.com)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

National Institutes of Health (NIH) Frequently Asked Questions (FAQs) Bone Marrow Transplantation and Peripheral Blood Stem Cell Transplantation: Questions and Answers about these Adult/NONembryonic Stem Cells Key Points Hematopoietic or blood-forming stem cells are. (physiciansforlife.org)
Somatic cell nuclear transplantation has become a focus of study in stem cell research. (wikipedia.org)
Even though each cadaveric organ donor can often supply multiple organs for transplantation, many patients still die before a suitable organ becomes available. (scialert.net)
1999). Majority of the organs for transplantation are donated from patients in whom brain-stem death has been diagnosed and who are then ventilated to maintain adequate oxygenation and circulation-the so called non-heart-beating donors (NHBDs) (D Allessandro et al . (scialert.net)
1995). Though this speaks volumes about the improvement in the level of health-care being provided, it also points to the important fact that the number of potential donors may be decreasing, which in turn is seriously limiting the transplantation programs. (scialert.net)
The Law on Transplantation of Organs, Tissues, and Cells (promulgated SG No. 83/19 September 2003, into force 1 January 2004) regulates the use, collection and storage (including biobanks) of all cells, organs and human tissue in Bulgaria. (eurostemcell.org)
Law on the Transplantation of Organs, Tissues and Cells (Promulgated in the State Gazette, issue No. 83/19 September 2003, entered into force on 1 January 2004), at http://bgtransplant.bg/iat/docs/Law%20on%20transplantation.doc (Word download), accessed 10 October 2011. (eurostemcell.org)
Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. (nature.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
We predict the time taken for mutant clones to expand within a host, as well as chimerism levels that can be expected following transplantation therapy, and the probability that a preconditioned host is reconstituted by donor cells. (uzh.ch)
Cross-species transplantation was possible without the rejection of the human embryonic stem cells by the mice's immune systems because the mice were genetically modified to suppress certain immune responses that would have interfered with transplantation. (citizendium.org)
Hematopoietic stem cell transplantation (HSCT) results in the alteration of several components of the immune system. (medscape.com)
Transplantation can result in granulocytopenia as well as impairment of barrier defenses, cell-mediated immunity, and humoral immunity. (medscape.com)
In autologous transplantation, the donor and recipient is the same individual. (medscape.com)
When the donor is someone other than the recipient, the procedure is described as allogeneic transplantation. (medscape.com)
Together, both IST and stem cell transplantation are reasonable treatment options for patients with AA. (springermedizin.at)
Donor cell-derived leukaemia (DCL) is an uncommon complication of allogeneic hematopoietic stem cell transplantation (HSCT). (lidsen.com)
Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)

Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)

Development will ensue normally and after many mitotic divisions, the single cell forms a blastocyst (an early stage embryo with about 100 cells) with an identical genome to the original organism (i.e. a clone). (wikipedia.org)
These cells genetically matched the donor organism from which they came. (wikipedia.org)
These cells are deemed to have a pluripotent potential because they have the ability to give rise to all of the tissues found in an adult organism. (wikipedia.org)
These stem cells are genetically matched to the donor organism, holding promise for studying genetic disease. (eurostemcell.org)
Internal signals producing apoptosis depend on interactions of several proteins and may serve to protect the organism from cancer by killing cells that have pre-cancerous changes. (agemed.org)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
A clone is an organism that is a genetic copy of an existing one. (who.int)
XI - embryonic stem cells: embryonic cells that are capable of modifying the cells of any organism tissue. (hinxtongroup.org)
… "embryo" means a human organism during the first 56 days of its development following fertilization or creation, excluding any time during which its development has been suspended, and includes any cell derived from such an organism that is used for the purpose of creating a human being. (hinxtongroup.org)
A new organism is created by asexual reproduction using a duplicate of a single cell from the parent organism. (geminigenetics.com)
This is the most known form of cloning and involves creating a genetically identical replica of a whole organism. (geminigenetics.com)
The process of reproductive cloning involves the nucleus of a somatic (body) cell from a donor organism to be cloned being transferred into an egg cell whose nucleus (genetic material) has been removed. (geminigenetics.com)

Lee Byeong-chun and Kang Seung-keun have been charged with faking, together with Wang Woo-suk, the results of embryonic stem cell research and of embezzling public funds for the studies. (asianews.it)
Human embryonic stem cell research and the derivation of human embryonic stem cell lines is not directly regulated by Bulgarian legislation, but falls partially within the scope of the Bulgarian Health Act (promulgated SG No. 70/10 August 2004, into force from 1 January 2005). (eurostemcell.org)
Peter Braude, Professor of Obstetrics and Gynaecology at Kings College London, who has also been granted HFEA licences for embryonic stem cell research in the past commented, 'This is a difficult situation because there is a strong need for eggs for research. (progress.org.uk)
However, supporters of embryonic stem cell research frequently contend that even the comparison to abortion is inappropriate, since while a several month old fetus might have sufficient neurological development to be conscious in some meaningful sense, a human embryo in the blastocyst stage has so little development that one can safely conclude that it cannot exist as a conscious being. (citizendium.org)

Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)

Scientists produced embryonic stem cells from the DNA of one person combined with a human donor egg. (technologyreview.com)
In January 2018, a team of scientists in Shanghai announced the successful cloning of two female crab-eating macaques (named Zhong Zhong and Hua Hua) from foetal nuclei. (wikipedia.org)
In the United States, scientists at the Harvard Stem Cell Institute, the University of California San Francisco, the Oregon Health & Science University, Stemagen (La Jolla, CA) and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce embryonic stem cells. (wikipedia.org)
Since stem cells have the ability to turn into various other types of cells, scientists believe that they can be useful for treating and understanding diseases. (healthline.com)
To create iPSCs, scientists genetically reprogram the adult stem cells so they behave like embryonic stem cells. (healthline.com)
Scientists are hoping that the cells can be made from someone's own skin to treat a disease. (healthline.com)
Our lab has been tasked with converting control and patient blood or skin cells, collected by scientists throughout the state of California, into high quality iPSCs. (ca.gov)
ISCO will simultaneously partner with leading scientists across the world to showcase the therapeutic applicability and the potential advantages of hPSC over other stem cells. (pharmaceutical-technology.com)
Yesterday, scientists in the United Kingdom announced that they'd been granted permission by the Human Fertilization and Embryology Authority to create stem cells by therapeutic cloning. (sentientdevelopments.com)
In therapeutic cloning, scientists take a human egg from a healthy donor, and remove its nucleus. (sentientdevelopments.com)
Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
The exact process of differentiation is not yet understood and although embryonic stem cells can, in principle, provide for all human tissue, scientists are some way from controlling the process. (spiked-online.com)
It might be expected that the richest nation on Earth would encourage its top scientists to pursue this work with vigor rather than limiting funding opportunities, creating legal barriers and fencing off any newly developed cell lines. (spiked-online.com)
Long before the controversy emerged over human embryonic stem cells, scientists and doctors began using first-generation stem cells from adult bone marrow. (eppc.org)
But they are also less equipped to produce every cell type of the body and less able to reproduce themselves indefinitely, which makes them less appealing to scientists interested in basic research. (eppc.org)
In July 2005, for example, scientists announced that they had engineered adult mouse stem cells into usable mouse eggs, a technique that might one day allow for the creation of human eggs from ordinary human cells. (eppc.org)
Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
1. Cloning is an umbrella term traditionally used by scientists to describe different processes for duplicating biological material. (who.int)

In genetics and developmental biology, somatic cell nuclear transfer (SCNT) is a laboratory strategy for creating a viable embryo from a body cell and an egg cell. (wikipedia.org)
Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning the clone embryo is implanted into a host mother for further development and brought to term. (wikipedia.org)
The aim of carrying out this procedure is to obtain pluripotent cells from a cloned embryo. (wikipedia.org)
Embryonic stem cells are undifferentiated cells of an embryo. (wikipedia.org)
During the process of harvesting embryotic stem cells, the embryo is destroyed. (healthline.com)
After many divisions in culture, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original donor who provided the adult cell - a genetic clone. (eurostemcell.org)
The egg then "reprograms" the adult nucleus so that the cell behaves like an embryo but has the genes of the adult cell. (nih.gov)
When an embryo like this is implanted into a uterus, as with Dolly, the process is called reproductive cloning. (nih.gov)
In another strategy, called therapeutic cloning, the embryo can instead be used to create stem cells that are genetically identical to a patient. (nih.gov)
Since embryonic stem cells have the ability to form virtually any cell type in the body, those taken from a cloned embryo could potentially be used to treat many diseases. (nih.gov)
This process gets rid of unneeded cells and is particularly important for "sculpting" tissue and organ structure during development of the embryo (or larval metamorphosis in insects), but may occur at any time even in adult cells when a tissue needs to be remodeled. (agemed.org)
Research advocates attack President Bush for "banning stem cell research," while pro-life advocates lament a Republican administration and Congress that have banned nothing-not embryo destruction, not human cloning, not fetal farming, not genetic engineering. (eppc.org)
Before leaving office, President Clinton sought to get around the existing law without actually changing it, by funding research on embryonic stem cells so long as the actual embryo destruction was paid for with private dollars. (eppc.org)
Totipotent cells have the capacity to differentiate to all cell types, including somatic cells, germ cells, and certain cells that exist outside the embryo and are important to fetal development that are termed extraembryonic cells. (citizendium.org)
Such tissue renewal may be accomplished via the use of adult stem cells, or embryonic stem cells, which may be derived from a human embryo in the blastocyst stage. (citizendium.org)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
… "human clone" means an embryo that, as a result of the manipulation of human reproductive material or an in vitro embryo, contains a diploid set of chromosomes obtained from a single - living or deceased - human being, fetus, or embryo. (hinxtongroup.org)
The resulting embryo is then implanted into a surrogate mother, resulting in the birth of an animal genetically identical to the body cell donor. (geminigenetics.com)
Therapeutic cloning involves the creation of an early-stage embryo (blastocyst) and the removal of stem cells from the developing embryo. (geminigenetics.com)
An electrical impulse is then applied to the egg cell to stimulate it to become an embryo. (geminigenetics.com)
Though pet cloning may be considered a relatively new technology, the process of cloning as defined above is first documented in 1885, where Hans Adolf Eduard Driesch demonstrated artificial embryo twinning on a sea-urchin. (geminigenetics.com)

The blastocyst stage is developed by the egg to help create embryonic stem cells from the inner cell mass of the blastocyst. (wikipedia.org)
To produce Dolly, the cloned blastocyst was transferred into the womb of a recipient ewe, where it developed and when born quickly became the world's most famous lamb. (eurostemcell.org)
In therapeutic cloning, the blastocyst is not transferred to a womb. (eurostemcell.org)
Instead, embryonic stem cells are isolated from the cloned blastocyst. (eurostemcell.org)

2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
Cellular therapy based on mesenchymal stem cells (MSCs) is a promising novel therapeutic strategy for the osteonecrosis of the femoral head (ONFH), which is gradually becoming popular, particularly for early-stage ONFH. (biomedcentral.com)
RepliCel Life Sciences Inc. today announced that it has been granted a patent by Japan's Ministry of International Trade and Industry for hair follicle mesenchymal stem cells and their use thereof. (baldingblog.com)

Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
We also examined the numbers of colony-forming progenitor cells (CFUs) before and after therapy. (springermedizin.at)
The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

Somatic cell nuclear transfer is a technique for cloning in which the nucleus of a somatic cell is transferred to the cytoplasm of an enucleated egg. (wikipedia.org)

The stem cells could be studied in the laboratory to help researchers understand what goes wrong in diseases like these. (eurostemcell.org)
Using eggs from adult women who had previously donated for successful fertility treatments, the researchers used SCNT to transfer DNA into the egg cells. (the-scientist.com)
Researchers have been hoping to harness the therapeutic potential of cloning ever since the cloning of Dolly the sheep in 1997. (nih.gov)
Before this new study was published, Nature asked another group of researchers to confirm that the stem cells were genetically identical to the donor skin cells. (nih.gov)
The stem cells, the researchers showed, could turn into heart or nerve cells in the laboratory, and had other characteristics of established embryonic stem cell lines. (nih.gov)
In addition to making sure researchers end up with the plasmid they want and generating large combinatorial libraries, j5 addresses "all the considerations of traditional multiple cloning site technology. (genomeweb.com)
IEEE Pulse spoke with these two researchers about the study, their findings, and how they hope the results will impact stem-cell research and associated therapies. (embs.org)
However, researchers at the Harvard Stem Cell Institute say reprogrammed cells won't eliminate the need or value of studying embryonic stem cells. (cbc.ca)
Researchers have recently described new lines of stem cells derived from amniotic fluid [9] . (citizendium.org)
Researchers are making great strides with hair cloning, but I have no idea how many years it's going to be before anything reliably safe and effective is commercially available. (baldingblog.com)
Numerous biological components, including genes, cells, tissues, and even complete creatures like sheep, have been cloned by researchers, and now cat, dog and equine cloning is widely and reliably available via international companies such as our partner, ViaGen Pets & Equine. (geminigenetics.com)

One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
After the somatic cell transfers, the cytoplasmic factors affect the nucleus to become a zygote. (wikipedia.org)
The process of somatic cell nuclear transfer involves two different cells. (wikipedia.org)
The second being a somatic cell, referring to the cells of the human body. (wikipedia.org)
What is left is a somatic cell and an enucleated egg cell. (wikipedia.org)
These are then fused by inserting the somatic cell into the 'empty' ovum. (wikipedia.org)
After being inserted into the egg, the somatic cell nucleus is reprogrammed by its host egg cell. (wikipedia.org)
The resulting cells would be genetically identical to the somatic cell donor, thus avoiding any complications from immune system rejection. (wikipedia.org)
Cloning, or somatic cell nuclear transfer (SCNT), is the technique used to produce Dolly the sheep, the first animal to be produced as a genetic copy of another adult. (eurostemcell.org)
Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)
2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)

Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
Another application of SCNT stem cell research is using the patient specific stem cell lines to generate tissues or even organs for transplant into the specific patient. (wikipedia.org)
These stem cells come from developed organs and tissues in the body. (healthline.com)
The reproductive cloning of people is prohibited, including that for the purposes of donating organs, tissues and cells. (eurostemcell.org)
Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
Today, we can derive stem cells from a range of adult and newborn tissues: liver cells, kidney cells, brain cells, fat cells, and umbilical cord blood. (eppc.org)

A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patient's particular disease. (wikipedia.org)
For example, if a person with Parkinson's disease donated their somatic cells, the stem cells resulting from SCNT would have genes that contribute to Parkinson's disease. (wikipedia.org)
Two separate research teams have figured out how to "reprogram" cells with just a handful of genes to give them the characteristics of embryonic stem cells. (nih.gov)
This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and regulation of cloned genes can be followed within the context of a well characterized developmental programme. (nature.com)
Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
Cells become cancerous by accumulating, stepwise, a series of several mutations that alter the function of genes important for cell growth. (agemed.org)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
But previous approaches required the use of viruses to deliver the four genes needed to activate the cell and accomplish that task. (cbc.ca)
The precise pathophysiology of inherited single cell and multilineage cytopenias has not been elucidated despite the identification of many of the genes mutated in these disorders. (medscape.com)

Unlike SCNT, iPS cells are derived from adult cells, such as skin, which are reprogrammed to behave like embryonic stem cells. (medicalxpress.com)

If we can overcome this, they could certainly offer a great alternative to embryonic stem cells. (medicalxpress.com)
Such cells provide a potential alternative to embryonic stem cells. (citizendium.org)

These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
This gives them the ability to create patient specific pluripotent cells, which could then be used in therapies or disease research. (wikipedia.org)
The belief is that the use of stem cells in cell-replacement therapies will be a reality, so it's important to know whether the cells that are going to be put into a patient's body are full of deleterious mutations. (embs.org)
So we looked at three of these strategies to see if any of them induced deleterious mutations, and, if they did, we wanted to know whether those mutations actually had some kind of functional effects that would compromise resulting stem cells as cell-replacement therapies. (embs.org)
Ethically, since eventually all such "research" will be applied to people, he cautions against the abuse of women "egg" donors, and against the premature use of vulnerable sick human patients for testing supposedly "patient-specific" stem cells in supposed "therapies", pointing to the obvious violations of standard international research ethics guidelines such clinical trials would necessarily entail. (lifeissues.net)
In May 2007, Ontario and California announced a $30-million stem cell research deal aimed at finding new therapies for those diseases. (cbc.ca)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
Another long-term hope for therapeutic cloning is that it could be used to generate cells that are genetically identical to a patient. (eurostemcell.org)
The term "clone", from the Greek word for twig, denotes a group of identical entities. (who.int)
In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)
In syngeneic transplants, the donor is the identical twin of the recipient. (medscape.com)
The word "cloning" refers to a variety of procedures that may be used to create biological copies that are genetically identical to the original. (geminigenetics.com)
Asexual reproduction is a natural method used by certain plants, bacteria, and single-celled creatures to create genetically identical offspring, i.e. clones. (geminigenetics.com)
Humans and other mammals may produce natural clones, commonly referred to as identical twins. (geminigenetics.com)
Pet cloning is the process where a genetically identical twin is created of your original animal companion. (geminigenetics.com)
The surrogate mum carries the cloned pet for the gestation period and once ready, gives birth to the clone who will be an identical genetic twin to the original pet whose skin sample was used to make the nucleus of the donor egg cell. (geminigenetics.com)

This means they can potentially produce new cells for any organ or tissue. (healthline.com)
Procedures for the shipping, infectious disease testing, and processing of donor samples were successfully implemented with the seven Tissue Collectors. (ca.gov)
Cellular Dynamics International (CDI) has continued to generate passage 5 iPSC lines from donor samples submitted to us by Tissue Collectors throughout the state of California. (ca.gov)
Because of delays in receiving donor samples from our Tissue Collectors we have requested and received a one-year No Cost Extension to complete the activities associated with the grant by 30 November 2017. (ca.gov)
An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
Current screening of potential new drugs is done using cell lines derived from animals or 'abnormal' human tissue such as tumor cells. (spiked-online.com)
CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)
The term stem cell is also used in reference to any adult cells that are capable of assisting in the restoration of adult tissue via self-renewal. (citizendium.org)
The use of the pluripotent and/or self-renewing qualities of stem cells is believed to have therapeutic benefits for the regeneration of tissue in humans. (citizendium.org)
Therapeutic cloning refers to the production of embryonic stem cells for medicinal reasons, for example regenerative medicine and tissue replacement. (geminigenetics.com)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)

Every time that you are cloning a different enzyme or gene, you might have to use a different pair of restriction sites," he said. (genomeweb.com)
This topical collection on " Gene Editing " aims to provide a forum for discussions on the latest technical developments in the fields of general genome engineering technologies, including (i) the establishment of cell culture systems, and (ii) the development of established and emerging organismal models by CRISPR/Cas9 or similar genome engineering tools. (mdpi.com)
To create mouse stem cells with a conditional knockout of the humanized Snca gene, a previously obtained clone with the first Snca exon flanked by LoxP sites, was used. (eco-vector.com)
The CRISPR/Cas9-mediated homologous recombination system with donor DNA oligonucleotides of the human sites of the corresponding gene sites was used to humanize the fourth and fifth exons. (eco-vector.com)
As a result of the study, a strategy for CRISPR/Cas9-assisted homologous recombination in the genome of mouse embryonic stem cells has been developed to create a fully humanized Snca gene encoding α-synuclein, and the clone genome of mouse embryonic stem cells has been edited using a CRISPR technology. (eco-vector.com)
Cell engineering through gene editing is fundamentally a two-step bioprocess. (nature.com)
On e of the wardens protecting these animals in Chillingham Cattle Park, Denene Crossley, states how "being isolated, they've managed to essentially purify their gene pool, to the point where they're natural clones of each other. (geminigenetics.com)
Molecular cloning refers to the production of multiple copies of a DNA fragment or gene. (geminigenetics.com)
Gene cloning refers to the identification and duplication of a single gene or a DNA segment, for the intention of investigating its function or creating a particular protein. (geminigenetics.com)
Zettner MA, Flor S, Ludewig G, Wagner J, Robertson LW, Lehmann L. Quinoid metabolites of 4-monochlorobiphenyl induce gene mutations in cultured Chinese hamster v79 cells. (who.int)

Mouse embryonic stem cells are known to be tumorigenic - that is, they can grow uncontrollably and become cancerous. (spiked-online.com)

Only a handful of the labs in the world are currently using SCNT techniques in human stem cell research. (wikipedia.org)
However, more research is needed to help understand the potential uses of amniotic fluid stem cells. (healthline.com)
What is cloning, and what does it have to do with stem cell research? (eurostemcell.org)
This form of cloning is unrelated to stem cell research. (eurostemcell.org)
In particular, Cellular Dynamics International (CDI) has taken lease to approximately 5000 square feet of lab space at the Buck Institute for Research on Aging in Novato, CA. The majority of this space is located within the new CIRM-funded Stem Cell Research Building at the Buck Institute and was extensively reconfigured to meet the specific needs of this grant. (ca.gov)
Seoul (AsiaNews) Seoul National University (SNU) yesterday suspended two colleagues of Hwang Woo-suk, a vet and university professor charged with faking his research results on human cloning. (asianews.it)
The facility is also near leading Californian research institutions that have already collaborated with ISCO on basic stem cell biology and therapeutic applications. (pharmaceutical-technology.com)
Now the U.K. is positioned to lead the world in translating the potential benefits of stem-cell research into patients. (sentientdevelopments.com)
Alternatively, research using eggs may point the way to methods which mimic their properties using other human cells and chemical agents. (spiked-online.com)
Surprisingly, even though stem-cell research is currently underway in many labs around the world, this basic question has remained unanswered. (embs.org)
So Loring, a professor of developmental neurology at the Scripps Research Institute in La Jolla, California, discussed it with Nicholas Schork, a professor and the director of human biology at the J. Craig Venter Institute, also in La Jolla, and together they embarked on a comprehensive analysis to find out whether stem cells are safe or not. (embs.org)
Victoria and New South Wales have put aside their competitive interstate rivalry to collaborate on a stem cell research project, as announced by Innovation Minister Gavin Jennings and NSW Minister for Science and Medical Research, Verity Firth, today. (medicalxpress.com)
Mr Jennings said changes to the Victorian Infertility Treatment Act and the NSW Human Cloning and Other Prohibited Practices Act have opened up new opportunities for stem cell research in Australia. (medicalxpress.com)
Australia is already a global leader in overall stem cell research and this new and clear regulatory framework gives us an opportunity to extend our leadership into SCNT which could transform how we treat diabetes, heart diseases and Parkinson's," Mr Jennings said. (medicalxpress.com)
Finally, and inexorably, a true professional scientist poses clearly challenging questions to his research colleagues, and to the scientific enterprise in general, about the dubious "scientific" justification for the current rush to clone human beings - for both "therapeutic" and for "reproductive" purposes. (lifeissues.net)
And he also agrees that if we don't find global agreement on human cloning, "we can probably expect dire consequences for the future of biomedical research and its impact on society at large. (lifeissues.net)
But he is equally concerned about the unethical aspects inherent in the rush to perform " therapeutic " human cloning research, including the abuses to all vulnerable human patients who would be required to participate in clinical trials. (lifeissues.net)
As he has questioned the HFEA before, would not the use of vulnerable human patients in clinical trials be premature, dangerous, and unethical given the already acquired knowledge in the research community that such supposed "patient-specific" stem cells would most probably cause serious immune rejection reactions in these patients? (lifeissues.net)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
A controversial scheme to extend the practise of 'egg sharing' has been approved by the UK's Human Fertilisation and Embryology Authority (HFEA) to provide greater numbers of eggs for embryonic stem (ES) cell research. (progress.org.uk)
It is hoped that one donor per week will be recruited and that each new recruit will donate six to ten eggs for the research. (progress.org.uk)
Professor Alison Murdoch who leads the Newcastle team said, 'It is of paramount importance to ensure that all donors are not recruited to participate in this research against their best interest by coercion or excessive financial inducement. (progress.org.uk)
These moral perils are surely not a reason to oppose adult stem cell research, which deserves vigorous and expanded public support. (eppc.org)
In December 1999, the editors of Science, the journal devoted to scientific and medical matters, called stem cell research the 'Breakthrough of the Year. (cbc.ca)
Since then, there has been a flurry of announcements about developments in stem cell research and hints of promising treatments for diseases such as Alzheimer's, Parkinson's and cancer. (cbc.ca)
Ontario and California together account for about 70 per cent of the stem cell research currently conducted in North America. (cbc.ca)
Some of that money would be aimed at turning the state into the second-largest stem cell research region in the United States. (cbc.ca)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
The aim of the study was to create a genetically modified clone of mouse stem cells with a conditional knockout of humanized α-synuclein, which can be used for the reinjection into mouse blastocysts, as well as for basic and applied in vitro research in the field of pathophysiology and neuropharmacology. (eco-vector.com)
Great Iranian Muslim scholars netics, stem cell research, and organ trans- laid huge emphasis on teaching and practis- plantation are some of the medical issues ing ethics. (who.int)

Usually organs are retrieved from only about 15-20% of the eligible cadaveric donors available each year. (scialert.net)
However, there are a number of factors limiting the procurement of organs and accordingly, therapeutic cloning that perhaps can yield still better results needs to be considered as an alternative. (scialert.net)
They are in an early stage of development and have the ability to become any type of cell to form skin, bones, organs or other body parts. (cbc.ca)

Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)

General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)
WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)

This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
In 1996, Dolly the sheep became famous for being the first successful case of the reproductive cloning of a mammal. (wikipedia.org)
The cloning of 'Dolly The Sheep' in 1996 by the Rosalind Institute in Scotland, UK, is the most recognised example of reproductive cloning. (geminigenetics.com)

iPSCs have the great advantage that they can be generated from adults suffering from a known disease and then converted ("differentiated") into any cell type in the body. (ca.gov)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

Embryonic stem cells are immortal, and have the potential to develop into any type of adult cell, even after months growing in culture dishes. (news-medical.net)
The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)
The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
For example, stem cells could be generated using the nuclear transfer process described above, with the donor adult cell coming from a patient with diabetes or Alzheimer's. (eurostemcell.org)
Cloning entails taking the nucleus - the compartment that contains the DNA - from an adult cell and putting it into an egg from which the original nucleus has been removed. (nih.gov)

The genetic material of the donor egg cell is removed and discarded, leaving it 'deprogrammed. (wikipedia.org)
The key is that the DNA, the genetic material in those embryonic stem cells, comes from the patient, whose immune system won't reject the stem cells. (sentientdevelopments.com)
Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
The DNA within the skin sample is cultured and inserted into a donor egg cell whose nucleus (genetic material) has been removed. (geminigenetics.com)

Doctors have been performing stem cell transplants, also known as bone marrow transplants, for decades using hematopoietic stem cells in order to treat certain types of cancer. (healthline.com)
Donors for allogeneic transplants may be related or unrelated to the recipient. (medscape.com)
Allogeneic transplants are further categorized by the degree of human leukocyte antigen (HLA) match between the donor and recipient. (medscape.com)

Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
The transplant lobby considers this a big problem because administering anesthesia is perceived as an additional difficulty in telling the relatives that the donor is not actually dead but in an unsurvivable coma. (scialert.net)
Could a Hair Transplant Today Cause Problems for Any Potential Cure or Cloning in the Future? (baldingblog.com)
If hair cloning is a viable option in the future (perhaps in 15-20 years since I know the time line keeps moving every year) would you expect to see a large number of people elect to have a hair transplant for the sole reason of increasing overall hair density? (baldingblog.com)
The transplant procedure requires the harvesting of hematopoietic stem cells from a donor. (medscape.com)
We used a transplant model to determine whether CD341 cells migrate from the bone marrow to Ewing s sarcoma tumors and participate in the neovascularization process that supports tumor growth. (conicet.gov.ar)

SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
In human SCNT experiments, these eggs are obtained through consenting donors, utilizing ovarian stimulation. (wikipedia.org)
SCNT, or therapeutic cloning, is one method used to produce a source of individually-tailored stem cells. (medicalxpress.com)
Once our team produces the iPS cells, we will compare the properties of iPS versus cells developed through SCNT. (medicalxpress.com)

The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
CD341 bone marrow stem cells can differentiate into endothelial and hematopoietic cells. (conicet.gov.ar)
These data show that bone marrow stem cells contribute to the growing tumor vasculature in Ewing s sarcoma and that VEGF165 is critical for the migration of CD341 cells from the bone marrow into the tumor. (conicet.gov.ar)

This produces either heterozygous human parthenogenetic stem cell lines that can be precisely HLA-matched or stem cell lines histocompatible with the oocyte donors or HLA homozygous hPSC that are histocompatible with the major segments of human population. (pharmaceutical-technology.com)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)

Many of these donors suffer from a variety of multigenic disorders and high-quality iPSC lines should prove invaluable in improving our understanding of these devastating diseases in addition to providing a starting point for the discover of effective therapeutics. (ca.gov)
The hpSC cell line will be used to treat a host of degenerative diseases. (pharmaceutical-technology.com)
Already, non-embryonic stem cells are being used to treat a variety of diseases-most notably certain cancers of the blood. (eppc.org)
The recovered stem cells can then be used in the treatment of diseases and to aid the recovery of injuries. (geminigenetics.com)
They can develop into any kind of mature functional cell and thus help cure many degenerative and auto-immune diseases. (earthtomarrakech.org)

The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
In particular, the efficiency of the process will have to be improved before the technique could be applied in the clinic using human cells. (nih.gov)
Furthermore, the use of diphtheria toxin -- which is far more toxic to human cells than mouse cells -- to destroy the human neurons in the mice reversed the observed improvements in motor function. (citizendium.org)
The tumor sections expressed both human VE-cadherin and mouse CD31, indicating involvement of donor-derived human cells in the tumor vessels. (conicet.gov.ar)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)

Aplastic anemia is a disorder of the hematopoietic stem cell that results in a loss of blood cell precursors, hypoplasia or aplasia of bone marrow, and cytopenias in two or more cell lines (red blood cells, white blood cells, and/or platelets). (msdmanuals.com)

Science retracted both articles after the SNU revealed that the studies were faked, and that the stem cells had not been genetically created but had come from donors' eggs. (asianews.it)
Unfertilised human eggs are used to produce parthenogenic stem cells (hpSC) that have the ability to be immune-matched with millions of patients. (pharmaceutical-technology.com)

Cancer A clonal growth (cells all descended from one ancestral cell) that undergo continuing mitotic divisions and are not inhibited in their growth when they come in contact with neighboring cells (contact inhibition). (agemed.org)
Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. (nature.com)
Each colony is split and sequentially exported for on-target sequencing and further off-chip clonal expansion of the validated clones. (nature.com)

The technique consists of taking an denucleated oocyte (egg cell) and implanting a donor nucleus from a somatic (body) cell. (wikipedia.org)

Researcher Jeanne Loring thinks she has a good method for reversing the symptoms of Parkinson's disease, and she believes this method-a stem-cell therapy-will find its way to clinics in as little as twoand- a-half years. (embs.org)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)

The mitochondrial DNA of the stem cells, however, matched the donor egg's mitochondrial DNA. (news-medical.net)
Known as mitochondrial allostatic load, this (phenomenon) occurs when mitochondria are unable to recalibrate and maintain cell homeostasis. (bvsalud.org)

The disease specific stem cell lines could then be studied in order to better understand the condition. (wikipedia.org)
To date, no human embryonic stem cell lines have been derived using therapeutic cloning, so both these possibilities remain very much in the future. (eurostemcell.org)
The team that isolated the embryonic stem cell lines was led by Dr. Shoukhrat Mitalipov at Oregon Health and Science University in Portland. (nih.gov)
First year progress on grant ID1-06557, " Generation and Characterization of High-Quality, Footprint-Free Human Induced Pluripotent Stem Cell (iPSC) Lines From 3000 Donors to Investigate Multigenic Disease" has met all agreed-upon milestones. (ca.gov)
For example, iPSC lines from patients with heart disease can be converted into heart cells, iPSC lines from patients with Alzheimer's disease can be converted to brain cell, and iPSC lines from patients with pulmonary fibrosis can be converted into cells of the lung. (ca.gov)
Comparison of these "patient-derived" iPSC lines with those from healthy donors can help to illuminate the underlying cause of disease and also to serve as a system for discovering drugs to treat the disease in question. (ca.gov)
Banking of multiple cell lines with varying genetic spectrum that can be matched to patients is one possibility. (spiked-online.com)
Currently, iPS cell lines show variability in their potential to produce mature cells. (medicalxpress.com)
Both methods need to be investigated further so we can determine which will produce the most robust cell lines," Dr Verma said. (medicalxpress.com)
Professor Tuch's team will also use their proficiencies in characterising human embryonic stem cell lines and bioinformatics. (medicalxpress.com)
shows with high certainty that every NT-hESC line derived here originated from the respective patient donor and that these lines were not the result of enucleation failures and subsequent parthenogenetic activation. (citizendium.org)
The other is that co-authors Jin-Soo Kim at Seoul National University in South Korea and Juan Carlos Belmonte at the Salk institute in California - both pioneers of the CRISPR technique - had meticulously optimized the choice of 'guides' for the CRISPR editor by testing them in iPS cell lines that carried the same genetic fault. (cosmosmagazine.com)
Moreover, even when high-purity single-cell sorting can be achieved, viability after sorting is often low to moderate, especially for cell types that are particularly sensitive to hydrodynamic stress or low-density culture conditions (e.g., primary cells or pluripotent stem cell lines). (nature.com)
Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

The stem cell facility will produce human parthenogenic stem cells (hpSCs) that are derived from parthenogenetically induced human oocytes. (pharmaceutical-technology.com)
The HLA genotypes of stem cells obtained from such oocytes are heterozygous and are genetically cloned to the donors. (pharmaceutical-technology.com)

The stem cell source may be bone marrow, peripheral blood, or umbilical cord blood. (medscape.com)

All humans start out as only one cell. (healthline.com)
In most countries, it is illegal to attempt reproductive cloning in humans. (eurostemcell.org)
Review of Critical Article: Cobbe, 'Why the apparent haste to clone humans? (lifeissues.net)
See Neville Cobbe, "Why the apparent haste to clone humans? (lifeissues.net)
Properly controlled, adequately sized studies have yet to demonstrate that human embryonic stem cells have medical value in humans. (citizendium.org)

Stem cells have also been found in amniotic fluid. (healthline.com)

NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
We also analyse the engraftment of donor stem cells into non-conditioned and conditioned hosts, quantifying the impact of different treatment scenarios. (uzh.ch)

Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
There are major obstacles to overcome before a stem cell line is liable to reach clinical trials. (spiked-online.com)
That's also true for a researcher who may not be using stem cells for clinical applications but is using them in assays studying phenomena in a petri dish or in an animal model. (embs.org)
The first clinical trials involving a patient receiving human embryonic stem cells began in October 2010 at the Shepard Center, a spinal cord injury hospital in Atlanta. (cbc.ca)
No obvious correlations between clinical parameters, including age, karyotype, existence of paroxysmal nocturnal hemoglobinuria clones, pretreatment blood counts, progenitor cell counts, and the response to immunosuppressive therapy (IST), were found. (springermedizin.at)
BACKGROUND: A group of genetically altered cells that have not transformed into a clinical or histologically identifiable state of malignancy but contains a higher risk of transforming into one is known as the field of cancerization. (bvsalud.org)
We will also explore whether lower MIH will be related to higher cell-free mtDNA (ccf-mtDNA) levels and poor clinical outcomes. (bvsalud.org)
A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)

The downstream step involves identification and selection of the cells that have been properly edited. (nature.com)
The precise mechanism remains unclear, but in the majority of acquired cases, the mechanism involves an immune attack on the hematopoietic stem cell. (msdmanuals.com)

They put the nucleus of the patient's cell into the egg whose nucleus has been removed. (sentientdevelopments.com)

In the upstream step, delivery of genome editing machinery to the cell type of interest generates efficient and specific edits. (nature.com)
When genome editing induces a phenotypic alteration that is detectable by fluorescence (i.e., cell-surface expression of a target that can be non-lethally assessed with fluorescently labeled antibody), FACS provides a method of enriching edited cells 9 , narrowing the number of clones to propagate and analyze. (nature.com)
Therefore, the development of a method that allows screening of edited cells and minimizes cell manipulation and hands-on culturing would constitute a significant addition to the current genome engineering toolbox. (nature.com)

Anatomy48

Organisms20

Diseases9

Chemicals and Drugs40

Analytical, Diagnostic and Therapeutic Techniques and Equipment73

Phenomena and Processes63

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science4

Named Groups3

Health Care2

Embryos33

Pluripotent stem11

Transplantation20

20231

Organism12

Embryonic stem cel4

Hematopoietic cell1

Scientists20

Embryo21

Blastocyst4

Mesenchymal stem7

Progenitor cells8

Nucleus of a somatic1

Researchers11

Somatic cell14

Reproductive cloning of huma5

Tissues7

Genes9

Behave like embryonic stem1

Alternative to embryonic stem2

Therapies10

Identical12

Tissue15

Create embryonic stem1

Gene10

Mouse embryonic stem1

Research30

Organs3

Differentiation2

19972

Dolly3

IPSCs2

Adult cell5

Genetic material4

Transplants3

Transplant6

SCNT6

Bone marrow stem cells3

Parthenogenetic stem cell2

Diseases5

Human Cells5

Aplastic anemia1

Eggs2

Clonal3

Oocyte1

Parkinson's2

Mitochondrial2

Lines14

Oocytes2

Umbilical cord1

Humans5

Amniotic fluid1

Engraftment3

Clinical10

Autologous3

Involves2

Patient's1

Genome3