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  • recombinant
  • A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. (osti.gov)
  • Superfamily
  • Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. (nih.gov)
  • since MFS proteins facilitate the transport of many different substrates, the residues involved in substrate binding may not be strictly conserved among superfamily members. (nih.gov)
  • membranes
  • MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. (nih.gov)
  • eukaryotes
  • About 200 domains in eukaryotes occur in diverse architectures and provide a challenge for phylogenetic inference, as proteins can be composed of non-homologous elements. (plos.org)
  • interacts
  • ALP includes both an N-terminal PDZ domain, which interacts with the α-actinin-2, and a C-terminal LIM domain. (pubmedcentralcanada.ca)
  • At subsequent stages of development, DLT interacts with the apical determinant Crumbs (CRB) and the laterally localized protein Neurexin IV (NRX IV). (nih.gov)
  • RCF3 interacts with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2), ultimately affecting the phosphorylation of one of the main DICER-LIKE1 (DCL1) accessory proteins, HYPONASTIC LEAVES1 (HYL1), with a concomitant effect on miRNA production. (pnas.org)
  • Findings
  • Our findings predict that cell-specific mutant phenotypes caused by loss of LEM-D proteins reflect both the constellation of LEM-D proteins within the nuclear lamina and the capacity of functional compensation of the remaining LEM-D proteins. (genetics.org)
  • Annotation
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein. (uniprot.org)
  • patterns
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • Four ALP and three Enigma proteins have been identified in mammals, each having multiple splice variants and unique expression patterns. (pubmedcentralcanada.ca)
  • similarities
  • On the basis of similarities in protein structure, PDZ-LIM proteins have been divided into four subfamilies: Actinin-associated LIM protein (ALP) [ALP, CLP36, reversion-induced LIM (RIL) and Mystique], Enigma [Enigma, Enigma homologue protein (ENH) and Cypher/ZASP], LIM kinases (LMK1 and LMK2) and LMO7 ( te Velthuis and Bagowski, 2007 ). (pubmedcentralcanada.ca)
  • neuronal
  • ALP and Enigma proteins have been implicated in cardiac and skeletal muscle structure, function and disease, neuronal function, bipolar disorder, tumor growth, platelet and epithelial cell motility and bone formation. (pubmedcentralcanada.ca)
  • motif
  • This adaptor protein is known to contain a clathrin-binding motif and a motif that recognizes Dab2 cargoes like integrin β1. (fredhutch.org)
  • distinct
  • This conclusion is supported by studies showing that ectopically produced LEM-D proteins have distinct capacities to rescue the tissue-specific phenotypes found in single lem-d mutants. (genetics.org)
  • nuclear
  • THE nuclear lamina is an extensive protein network underlying the nuclear envelope. (genetics.org)
  • Recent evidence suggests that some LEM-D proteins are not enriched in the nuclear lamina, but shuttle between the cytoplasm and nucleus. (genetics.org)
  • In a first step, DCL1, aided by cofactors including the C2H2-zinc finger protein SERRATE (SE), the nuclear cap-binding complex (CBC), the double-stranded RNA-binding protein HYPONASTIC LEAVES1 (HYL1), and the HYL1 phosphatase C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 (CPL1), removes the two single-stranded RNA tails of the pri-miRNAs to form a largely double-stranded miRNA precursor (premiRNA) ( 2 ⇓ ⇓ - 5 ). (pnas.org)
  • concentration
  • The SP Sepharose and Q Sepharose column fractions can be rapidly assayed by using the Bio-Rad protein concentration reagent to identify the peak fractions. (bioon.com)
  • Protein concentration is above or equal to 0.05 mg/ml. (abcam.com)
  • amino
  • The LIM domains, represented by white ovals, are predominately localized at the amino terminus (left), however, some can be found at the carboxyl terminus (right). (sigmaaldrich.com)
  • MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. (nih.gov)