Domain carbohydrate sequence. Medical search. Frequent questions

CBMs are classified into numerous families, based on amino acid sequence similarity. (wikipedia.org)
Many putative CBMs have been identified by amino acid sequence alignments but only a few representatives have been shown experimentally to have a carbohydrate-binding function. (wikipedia.org)
Multidimensional heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the tertiary structure of the 152 amino acid N-terminal cellulose-binding domain from C. fimi 1,4-beta-glucanase CenC (CBDN1). (wikipedia.org)
We knew that these broadly neutralizing antibodies existed for many years, but it came as a great surprise that they were directed to carbohydrate epitopes rather than the more common amino acid epitopes," Berman said. (sciencedaily.com)
Analysis of their deduced amino acid sequences revealed several important differences from prototypic subtype B strains, including changes in the number and distribution of cysteine residues, substantial length differences in hypervariable regions, and premature truncations in the gp41 domain. (duke.edu)
One of these, derived from a patient with rapid CD4 cell decline, contained an amino acid substitution in a highly conserved endocytosis signal (Y721C), as mediated virus entry with very poor efficiency, although they did not contain sequence changes predicted to alter protein function. (duke.edu)
Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). (rndsystems.com)
Amino acid sequence and carbohydrate structure of a recombinant human tissue factor pathway inhibitor expressed in Chinese hamster ovary cells: one N-and two O-linked carbohydrate chains are located between Kunitz domains 2 and 3 and one N-linked carbohydrate chain is in Kunitz domain 2. (expasy.org)
Contiguous amino acid sequences within a carbohydrate-active enzyme with discrete folds having carbohydrate binding activity. (bvsalud.org)
They are classified into 43 different families based on AMINO ACID SEQUENCE, binding specificity, and structure. (bvsalud.org)

The enzymes contain ~250-300 residues, which encode putative signal sequences and carbohydrate attachment sites. (embl.de)

In molecular biology, a carbohydrate-binding module (CBM) is a protein domain found in carbohydrate-active enzymes (for example glycoside hydrolases). (wikipedia.org)
More recently, anaerobic fungi, typified by Piromyces equi, have been suggested to also synthesise a cellulosome complex, although the dockerin sequences of the bacterial and fungal enzymes are completely different. (wikipedia.org)
For example, the fungal enzymes contain one, two or three copies of the dockerin sequence in tandem within the catalytic polypeptide. (wikipedia.org)
The carbohydrate-active enzymes database (CAZy", NUCLEIC ACIDS RES. (sumobrain.com)
The majority of Xyn10C-like enzymes shared the same interrupted domain architecture and were vastly distributed in different xylan utilization loci found in gut Bacteroidetes, indicating the importance of this enzyme in glycan acquisition for gut microbiota. (hal.science)

but in anaerobic fungi they are protein binding domains, referred to as dockerin domains. (wikipedia.org)
This emphasizes the need to consider factors other than direct protein-inhibitor interactions to guide the design of domain-selective ACE inhibitors, especially in the case of larger peptides. (rcsb.org)
Click on the protein counts, or double click on taxonomic names to display all proteins containing CLECT domain in the selected taxonomic class. (embl.de)
The part of an antigen that is recognized by an antibody is called an epitope, and it is usually a specific sequence of amino acids in the antigen protein. (sciencedaily.com)
Scientists studying antibodies from elite neutralizers, however, discovered that many of the most potent neutralizing antibodies actually recognize carbohydrate components called glycans that are attached to the gp120 envelope protein. (sciencedaily.com)
Proteinet visade sig t o m ha en isopeptidbinding, vilket är den första som observerats i ett protein uttryckt av en Gram-negativ bakterie. (umu.se)
Glycosylation refers to a sequence of events that take place in the Golgi body and the endoplasmic reticulum of the cells of the expression system (predominantly mammalian) that lead to post-translational addition and processing of carbohydrate or glycan moieties to the protein backbone (usually serine- or threonineâ linked glycosylation for O-linked glycans and asparagineâ linked glycosylation for N-linked glycans) (6). (chromatographyonline.com)
TIM barrel domain, Glyoxalase/fosfomycin resistance/dioxygenase domain, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily [InterProScan]. (ntu.edu.sg)
They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. (rndsystems.com)
is to generate an antibody-like molecule by combining framework sequences from a human mAb with sequences from a human protein that carries a target-recognition function. (coek.info)
The most common example of this type of fusion protein combines the hinge and Fc regions of an immunoglobulin (Ig) with domains of a cell-surface receptor that recognizes a specific ligand (Box 1). (coek.info)
Immunoadhesin structure The majority of immunoadhesins combine the hinge and Fc regions of an IgGl heavy chain with the extracellular domain (ECU) of a type I transmembrane protein, usually a receptor or an adhesion molecule (Fig. 1). (coek.info)
One conserved basic residue is identical in position with that of a conserved basic residue that mediates hyaluronate binding in the structurally related proteoglycan tandem repeat (PTR) domain in G1 and in link protein. (brighton.ac.uk)
The MUC1 protein, also known as polymorphic epithelial mucin or epithelial membrane antigen, has a large extracellular region, a transmembrane sequence, and a cytosolic domain. (medscape.com)
By recording the solvent proton spin relaxation rate over a wide range of magnetic field strengths, it is possible to Submitted November 8, 2004, and accepted for publication December 28, resolve (in the frequency domain) protein oligomers with 2004. (lu.se)

Due to a common structural element, the amino-terminal lectin-like domain, the selectins are able to bind to carbohydrate ligands. (biovendor.com)
Their biological roles, initially understood as limited to recognition of endogenous ("self") carbohydrate ligands in embryogenesis and early development, dramatically expanded in later years by the discovery of their roles in tissue repair, cancer, adipogenesis, and regulation of immune homeostasis. (nsf.gov)
This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)

The chemical reactions and pathways involving carbohydrate derivative. (ntu.edu.sg)

A strikingly distinct set of carbohydrate esterases (CE) are present in M. phaseolina , with the CE9 and CE10 families remarkably higher than any other fungi. (biomedcentral.com)
The current state of knowledge of diversity, classification and structure-function relationship of these three types of xylanolytic carbohydrate esterases is discussed with emphasis on important aspects of their future research relevant to their industrial applications. (inrae.fr)
Glucuronoyl esterases (GEs) belonging to the carbohydrate esterase family 15 (CE15) are involved in microbial degradation of lignocellulosic plant materials. (inrae.fr)

Sequence analysis showed details of the unusual domain organization of this enzyme. (hal.science)

Availability of genome sequences facilitated the identification of pneumococcal surface proteins bearing characteristic motifs such as choline-binding proteins (Cbp) and peptidoglycan binding (LPXTG) proteins. (biomedcentral.com)

These isoforms have extracytoplasmic sequences ranging from 391 to 552 amino acids long with numerous N-linked carbohydrate attachment sites. (beckman.com)

No InterPro domain matches or not analyzed. (cornell.edu)

This domain contains 4 conserved cysteine residues which are involved in the formation of two disulfide bonds. (wikipedia.org)
This suggests the involvement of these subsites in conferring C-domain-selective BPPb binding, in agreement with the mutagenesis results where unique residues governing differences in active site exposure, lid structure and dynamics between the two domains were the major drivers for C-domain-selective BPPb binding. (rcsb.org)
Mere disruption of BPPb interactions with unique S 2 and S 4 subsite residues, which synergistically assist in BPPb binding, was insufficient to abolish C-domain selectivity. (rcsb.org)
The combination of unique S 9 -S 4 and S 2 ' subsite C-domain residues was required for the favourable entry, orientation and thus, selective binding of the peptide. (rcsb.org)
This domain, which is known as the C-type lectin domain (CTL) or as the carbohydrate-recognition domain (CRD), consists of about 110 to 130 residues. (embl.de)
Alignment of 131 sequences from all seven groups in the CRD superfamily defined a consensus length of 136 residues, in which 32% of residues were conserved. (brighton.ac.uk)
Although the G3 CRD sequences agreed with this consensus, they also contained five fully conserved basic residues that are atypical of the CRD superfamily. (brighton.ac.uk)
Homology modelling showed that four of these residues are located on a surface region on the CRD that is separate from the Ca2+-binding residues involved in carbohydrate interactions. (brighton.ac.uk)
The alignment of 13 G3 SCR sequences with 101 sequences in the SCR superfamily showed good agreement with conserved residues in the SCR superfamily. (brighton.ac.uk)
It is concluded that both the CRD and SCR domains in G3 possess basic residues that are atypical of their superfamilies and might be related to function, and that the G3 CRD domain shows an evolutionary relationship to the PTR domain in G1. (brighton.ac.uk)

The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)

They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. (beckman.com)
Genomic sequencing of A. fulgidus suggests that three [Ni-Fe]-CODHs are present, a "bacterial" monomeric CooS and two archaeal CdhAB-type CODHs [ 9 - 11 ]. (hindawi.com)

CBMs were previously known as cellulose-binding domains. (wikipedia.org)
It consists of one catalytic domain, which is intercalated by two carbohydrate binding modules (CBMs) from family 4. (hal.science)

Some of these domains are found on cellulosomal scaffoldin proteins. (wikipedia.org)
Carbohydrate-binding module family 10 (CBM10) is found in two distinct sets of proteins with different functions. (wikipedia.org)
There are 74207 CLECT domains in 53821 proteins in SMART's nrdb database. (embl.de)
Taxonomic distribution of proteins containing CLECT domain. (embl.de)
The complete taxonomic breakdown of all proteins with CLECT domain is also avaliable . (embl.de)
Agrin-like glycoproteins and proteins with sugar-binding domains are also predominant. (biomedcentral.com)

structure of bacillus subtilis pdaa in complex with nag, a family 4 carbohydrate esterase. (berkeley.edu)

Chitinases are glycosyl hydrolases that catalyze the hydrolysis of the β-1,4 linkages in complex carbohydrates and those that contain GlcNAc. (researchgate.net)
1,4 linkages in complex carbohydrates and those that contain GlcNAc. (researchgate.net)
Lectins can be found in many organisms and are involved in a multitude of cellular processes that depend on specific recognition of complex carbohydrates. (embl.de)

Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil‑coil neck domain (aa 96‑136), a C‑type lectin-like domain (CTLD) of the type found in human DC‑SIGN and DC‑SIGN receptor (aa 165‑289), and a C-terminal Ca 2+ -dependent carbohydrate‑recognition domain (C-type CRD) (1). (rndsystems.com)

Structural basis of lectin-carbohydrate recognition. (embl.de)
The PAP20 glycopeptides were isolated by reversed phase high pressure liquid chromatography and subjected to the structural analyses by quadrupole time-of-flight electrospray ionization mass spectrometry and to the sequencing by Edman degradation. (nih.gov)
Carbohydrate microarray analyses, corroborated by structural considerations, show that TgMIC13, TgMIC1, and its homologue Neospora caninum MIC1 (NcMIC1) share a preference for α2-3- over α2-6-linked sialyl-N-acetyllactosamine sequences. (unl.pt)
The CRD and SCR domains in 13 G3 sequences were each subjected to structural analysis. (brighton.ac.uk)
Galectins are a family of ß-galactoside-binding lectins characterized by a unique sequence motif in the carbohydrate recognition domain, and evolutionary and structural conservation from fungi to invertebrates and vertebrates, including mammals. (nsf.gov)

Lectin-carbohydrate interactions: different folds, common recognition principles. (embl.de)

CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. (biolegend.com)
Solid-phase oligosaccharide synthesis and combinatorial carbohydrate libraries. (mpg.de)

In anaerobic bacteria that degrade plant cell walls, exemplified by Clostridium thermocellum, the dockerin domains of the catalytic polypeptides can bind equally well to any cohesin from the same organism. (wikipedia.org)
In contrast, all the C. thermocellum cellulosome catalytic components contain a single dockerin domain. (wikipedia.org)

To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)

So Berman's lab set out to produce antigens that would induce a strong antibody response to the glycan-dependent epitopes in the V1/V2 domain. (sciencedaily.com)
5] These carbohydrate epitopes may be antigenically different than those in normal breast cells. (medscape.com)
These 2 antibodies target epitopes located on the peptide core of the extracellular domain of MUC1. (medscape.com)

G3 is implicated in the intracellular processing of aggrecan and contains a C-type lectin carbohydrate recognition domain (CRD), frequent occurrences of a C-terminal short complement repeat (SCR) domain, and occasionally an N-terminal epidermal growth factor domain. (brighton.ac.uk)

Carbohydrate-binding module family 5 (CBM5) binds chitin. (wikipedia.org)
Both cleft A and cleft B can bind cello-oligosaccharides, laminarin preferentially binds in cleft A, xylooligosaccharides only bind in cleft A and beta1,4,-beta1,3-mixed linked glucans only bind in cleft B. Carbohydrate-binding module family 9 (CBM9) binds to crystalline cellulose. (wikipedia.org)
Det innebär att en eller flera fettsyror binds till proteinets N-terminal vilket senare utgör en signal till transportsystemet (LolACDE) att proteinet ska transporteras över periplasman. (umu.se)

Phylogenetic analyses of full-length env sequences indicated that the panel included members of all major sequence subtypes of HIV-1 group M (clades A to G), as well as an intersubtype recombinant (F/B) from an infected individual in Brazil. (duke.edu)

We found that the vaccine that gave partial protection in the RV 144 trial had very little of the kind of carbohydrate required to bind these antibodies. (sciencedaily.com)
After activation C3b can bind covalently, via its reactive thiolester, to cell surface carbohydrates or immune aggregates. (lu.se)

Here, we used site-directed mutagenesis, an ACE chimera and X-ray crystallography to unveil the molecular basis for C-domain-selective ACE inhibition by the bradykinin-potentiating peptide b (BPPb), naturally present in Brazilian pit viper venom. (rcsb.org)
We present the BPPb N-domain structure in comparison with the previously reported BPPb C-domain structure and highlight key differences in peptide interactions with the S 4 to S 9 subsites. (rcsb.org)
The high incidence of these replacements and their detection also in other cancer cell lines imply that the conserved tandem repeat domain of MUC1 is polymorphic with respect to the peptide sequence. (nih.gov)

Carbohydrate-binding module family 1 (CBM1) consists of 36 amino acids. (wikipedia.org)
The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. (beckman.com)

Carbohydrate-binding module family 6 (CBM6) is unusual in that it contains two substrate-binding sites, cleft A and cleft B. Cellvibrio mixtus endoglucanase 5A contains two CBM6 domains, the CBM6 domain at the C-terminus displays distinct ligand binding specificities in each of the substrate-binding clefts. (wikipedia.org)

Portable sulphotransferase domain determines sequence specificity of heparan sulphate 3-O-sulphotransferases. (mpg.de)

The CA 15-3 antigen (also known as MUC1, from which it is derived) represents sequences of mucins that are often overexpressed in malignant glandular cells, such as breast cancer. (medscape.com)

The prototypic immunoadhesin is a disulfidelinked homodimer, which resembles an IgGl molecule, but which lacks C,l domains and light chains. (coek.info)
2~). In the absence of light chains, deletion of the C,,l domain improves assembly and secretion of immunoadhesins by the host cell. (coek.info)

The mucin-like glycoprotein PSGL-1 (P-selectin Glycoprotein Ligand-1) has been cloned and sequenced. (biovendor.com)

This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)

Scholars@Duke publication: Molecular cloning and analysis of functional envelope genes from human immunodeficiency virus type 1 sequence subtypes A through G. The WHO and NIAID Networks for HIV Isolation and Characterization. (duke.edu)
To generate a more representative panel of genetically diverse envelope genes, we PCR amplified, cloned, and sequenced complete gp160 coding regions of 35 primary (peripheral blood mononuclear cell-propagated) HIV-1 isolates collected at major epicenters of the current AIDS pandemic. (duke.edu)

Carbohydrate-binding module family 2 (CBM2) contains two conserved cysteines - one at each extremity of the domain - which have been shown to be involved in a disulfide bond. (wikipedia.org)
Carbohydrate-binding module family 3 (CBM3) is involved in cellulose binding and is found associated with a wide range of bacterial glycosyl hydrolases. (wikipedia.org)
Carbohydrate-binding module family 4 (CBM4) includes the two cellulose-binding domains, CBD(N1) and CBD(N2), arranged in tandem at the N terminus of the 1,4-beta-glucanase, CenC, from Cellulomonas fimi. (wikipedia.org)
Carbohydrate-binding module family 11 (CBM11) is found in a number of bacterial cellulases. (wikipedia.org)

N-terminal domain, C-terminal domain, FGGY family of carbohydrate kinases [InterProScan]. (ntu.edu.sg)
transmembrane domain, AhpC/TSA family [InterProScan]. (ntu.edu.sg)

The majority of these domains have carbohydrate-binding activity. (wikipedia.org)
Carbohydrate binding ability may have evolved independently and sporadically in numerous unrelated families, where each evolved a structure that was conserved to fulfil some other activity and function. (embl.de)

Moreover, transiently expressed glycoprotein precursor molecules varied considerably in both size and carbohydrate content. (duke.edu)

One key to improving the vaccine, Berman said, came from independent lines of investigation pointing to the importance of antibodies that recognize a specific segment of gp120 known as the V1/V2 domain. (sciencedaily.com)

Anatomy1

Organisms3

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes14

Technology, Industry, Agriculture1

Information Science2

Health Care1

Amino acid10

Putative1

Enzymes5

Protein15

Ligands3

Derivative1

Esterases3

Enzyme1

Genome Sequences1

Attachment sites1

Interpro1

Residues10

Metabolism1

Genomic2

CBMs2

Proteins6

Esterase1

Complex carbohydrates3

Lectin-like domain1

Structural5

Folds1

Oligosaccharide2

Catalytic2

Species1

Epitopes3

Recognition domain1

Binds3

Analyses1

Bind2

Peptide3

Consists2

Endoglucanase1

Specificity1

Malignant1

Chains2

Ligand1

Differences1

Genes2

Module4

Family2

Activity2

Precursor1

Recognize1