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  • fatty
  • Beta-ketoacyl-ACP synthase is a highly conserved enzyme that is found in almost all life on earth as a domain in fatty acid synthase (FAS). (wikipedia.org)
  • Certain experiments have also suggested the presence of "fatty acid transport tunnels" within the beta-ketoacyl-ACP synthase domain that lead to one of many "fatty acid cavities", which essentially acts as the active site. (wikipedia.org)
  • genes
  • Upon sterol depletion, LXRs are inactive and SREBPs are cleaved, after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. (wikipedia.org)
  • substrate specificity
  • Structural analysis shows that this domain adopts a fold not predicted by its primary sequence, and indicates how this domain maintains its high degree of substrate specificity. (pnas.org)
  • Site-directed mutagenesis at D422 shows a change in the substrate specificity of the propionyl-CoA binding site, thus indicating this residue's importance in PCC's catalytic activity. (wikipedia.org)
  • transporter
  • A representative list of proteins belonging to the NaT-DC family can be found in the Transporter Classification Database. (wikipedia.org)
  • This article incorporates text from the public domain Pfam and InterPro IPR006667 As of this edit, this article uses content from "1.A.26 The Mg2+ Transporter-E (MgtE) Family", which is licensed in a way that permits reuse under the Creative Commons Attribution-ShareAlike 3.0 Unported License, but not under the GFDL. (wikipedia.org)
  • TycD &TycE have the highest similarity to members of the ATP-binding cassette (ABC) transporter family which aid in the transport of substrates across a membrane. (wikipedia.org)
  • coli
  • LamB as a carrier molecule for the functional exposition of IgG-binding domains of the Staphylococcus aureus protein A at the surface of Escherichia coli K12. (ugent.be)
  • Solubilization tags are used, especially for recombinant proteins expressed in chaperone-deficient species such as E. coli, to assist in the proper folding in proteins and keep them from precipitating. (wikipedia.org)
  • E. coli chaperonin proteins groES and groEL: essential for folding and assembly of human PCC heteromeric subunits c. (wikipedia.org)
  • homologous
  • The α-δ subunits of these enzymes are homologous to proteins encoded within the genomes of archaea, such as Pyrococcus abyssi (Cohen et al. (wikipedia.org)
  • The α-subunits of the oxaloacetate and methylmalonyl-CoA decarboxylases are homologous to many biotin-containing enzymes including (1) pyruvate carboxylases, (2) homocitrate synthases, (3) biotin carboxyl carrier proteins, (4) isopropylmalate synthases and (5) acyl-CoA carboxylase. (wikipedia.org)
  • STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain, including STARD5. (wikipedia.org)
  • recombinant protein
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • thioester
  • Each AT domain has an α-carboxylated CoA thioester (i.e. methylmalonyl-CoA) This specificity prevents non-essential addition of enzymes within the module. (wikipedia.org)
  • synthetase
  • Mupirocin reversibly binds to the isoleucyl t-RNA synthetase in Staphylococcus aureus and Streptococcus, resulting in inhibition of protein synthesis. (wikipedia.org)
  • synthesis
  • We described here methods for the solid-phase synthesis of peptides and their coupling to PCP domains, which may be useful for in vitro studies of cross-linking and related tailoring reactions during nonribosomal glycopeptide antibiotic biosynthesis. (uzh.ch)
  • hydrophobic
  • Most importantly, the interior of the protein encompasses a 26 × 12 × 11-Angstrom hydrophobic tunnel that is apparently large enough to bind a single cholesterol molecule. (wikipedia.org)
  • The biotin-binding pocket of PCC is hydrophobic and highly conserved. (wikipedia.org)
  • predominantly
  • In suspended human fibroblasts, IGF-I tracer binds predominantly to type I IGF receptors (inhibition by IGF-I much greater than insulin greater than B-IGF-I hybrid molecules). (jci.org)
  • organisms
  • Orthologs of the TMCO6 protein have been found among sequenced organisms with the exception of invertebrates, fungi, plants and bacteria. (wikipedia.org)
  • perpendicular
  • The noncanonical "perpendicular" binding observed potentially represents the general form of a kinetic intermediate. (ox.ac.uk)
  • Biotin and propionyl-CoA bind perpendicular to each other in the oxyanion hole-containing active site. (wikipedia.org)
  • structurally
  • These proteins are different from plant nonspecific lipid transfer proteins but structurally similar to small proteins of unknown function from Thermus thermophilus. (wikipedia.org)
  • We have structurally characterized this C-terminal domain and have discovered that it adopts the aryl-alkylamine- N -acetyl transferase (AANAT) fold, which is unprecedented in NRPS biology. (pnas.org)
  • enzymatic
  • StAR or STARD1) and lipids like phosphatidylcholine (phosphatidylcholine transfer protein, also called PCTP or STARD2) and have enzymatic activities. (wikipedia.org)
  • Protein tags may allow specific enzymatic modification (such as biotinylation by biotin ligase) or chemical modification (such as reaction with FlAsH-EDT2 for fluorescence imaging). (wikipedia.org)
  • affinity
  • The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. (patentsencyclopedia.com)
  • Affinity tags are appended to proteins so that they can be purified from their crude biological source using an affinity technique. (wikipedia.org)
  • This is because they share the same binding site, as well as binding affinity to the microtubule. (wikipedia.org)
  • conformation
  • The vancomycin aglycone contains three cross-links between aromatic amino acid side-chains, which stabilize the folded backbone conformation required for binding to the target D-Ala-D-Ala dipeptide. (uzh.ch)
  • The native conformation of the D' domain of vWF is not only required for factor VIII (FVIII) binding but also for normal multimerisation and optimal secretion. (wikipedia.org)
  • The Mg2+-bound state adopts a compact, globular conformation, which is stabilized by the coordination of a number of Mg2+ ions between these domains. (wikipedia.org)
  • selectivity
  • Importantly, in some cases, the P-2 residue formed interactions outside of the binding groove, providing insight into the influence of residues remote from the binding groove on selectivity. (ox.ac.uk)
  • catalysis
  • The BC and CT sites are approximately 55 Å apart, indicative of the entire BCCP domain translocating during catalysis of the carboxylation of propionyl-CoA. (wikipedia.org)
  • incubation
  • The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease. (patentsencyclopedia.com)
  • Modulation of insulinlike growth factor I binding to human fibroblast monolayer cultures by insulinlike growth factor carrier proteins released to the incubation media. (jci.org)
  • We postulated that during the binding incubation (5 h, 15 degrees C), IGF-I tracer partitioned between binding sites on the cell surface and IGF carrier proteins released to the incubation media. (jci.org)
  • In support of this hypothesis, carrier proteins were demonstrated in the media at the end of the binding incubation with fibroblast monolayers, and the concentration of unsaturated binding sites in the media correlated inversely with tracer binding to the cells. (jci.org)
  • adenylation
  • This is accomplished through the subdomains for adenylation (A), peptityl carrier protein (PCP), condensation (C), and depending on the amino acid position, an epimerization (E). The adenylation subdomain is used in activating the specific amino acid. (wikipedia.org)
  • membrane
  • Expression of mislocalized mutant syntaxin 3 in Madin-Darby canine kidney cells leads to basolateral mistargeting of apical membrane proteins, disturbance of tight junction formation, and loss of ability to form an organized polarized epithelium. (rupress.org)
  • These results indicate that SNARE proteins contribute to the overall specificity of membrane trafficking in vivo, and that the polarity of syntaxin 3 is essential for epithelial cell polarization. (rupress.org)
  • These targeting signals are thought to be recognized in the TGN or endosomes, which leads to the sorting of cargo proteins into transport vesicles destined for the apical or basolateral plasma membrane. (rupress.org)
  • Matveeva, E.A.;Price, D.A.;Whiteheart, S.W.;Vanaman, T.C.;Gerhardt, G.A.;Slevin, J.T. "Reduction of vesicle-associated membrane protein 2 expression leads to a kindling-resistant phenotype in a murine model of epilepsy. (uky.edu)
  • a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent. (patentsencyclopedia.com)
  • They are found bound to the outer membrane of mitochondria in most cell types in the body. (genecards.org)
  • Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. (wikipedia.org)
  • The single transmembrane domain of 22 (mostly) non-polar residues crosses the plasma membrane in a single alpha helix. (wikipedia.org)
  • These inorganic phosphates are then bound to carrier proteins which deliver the inorganic phosphates to a specific high-affinity transport system, known as the Pst system, which transports phosphate across the cytoplasmic membrane. (wikipedia.org)
  • These include large membrane-associated proteins with N-terminal ACB domains, multifunctional enzymes with both ACB and peroxisomal enoyl-CoA Delta(3), Delta(2)-enoyl-CoA isomerase domains, and proteins with both an ACB domain and ankyrin repeats. (wikipedia.org)
  • residues
  • Histone deacetylases (HDACs) are conserved metalloproteases which aim to remove acetyl groups from lysine residues of targeted proteins. (hindawi.com)
  • The ligand is bound through specific interactions with residues on the protein, most notably several conserved positive charges that interact with the phosphate group on the adenosine-3'phosphate moiety, and the acyl chain is sandwiched between the hydrophobic surfaces of CoA and the protein. (wikipedia.org)
  • structural
  • It can be activated by collectin proteins which recognize the structural pattern of polysaccharide on the surface of microorganisms. (scirp.org)
  • Other proteins have structural or mechanical functions, such as those that form the cytoskeleton, a system of scaffolding that maintains the cell shape. (wikipedia.org)
  • Both the disulfide bonds and calcium coordination are necessary for the structural integrity of the domain during the receptor's repeated trips to the highly acidic interior of the endosome. (wikipedia.org)
  • exons
  • Chimeric RNA, sometimes referred to as a fusion transcript, is composed of exons from two or more different genes and have the potential to encode novel proteins. (wikipedia.org)
  • The exons of these 25,000 genes can be spliced in many different ways to create countless combinations of RNA transcripts and ultimately countless proteins. (wikipedia.org)
  • modulates
  • Deposition of matricellular proteins modulates growth factor signaling and contributes to the spatial and temporal regulation of the reparative response. (jci.org)
  • lipid
  • Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. (wikipedia.org)
  • As such, many lipid signaling molecules cannot circulate freely in solution but, rather, exist bound to special carrier proteins in serum. (wikipedia.org)
  • enhancer
  • Together with histone acetylases (HATs), HDACs regulate acetylation level of histones (H3, H2AK5, H4K5, H4K12, H2B, H4K8, and H4K16) as well as some other proteins (p65 and myocyte enhancer factor 2) [ 5 ]. (hindawi.com)
  • Procollagen C-endopeptidase enhancer 1 precursor (Procollagen COOH- terminal proteinase enhancer 1) (Procollagen C-proteinase enhancer 1) (PCPE-1) (Type I procollagen COOH-terminal proteinase enhancer) (Type 1 procollagen C-proteinase enhancer protein). (yellowcouch.org)
  • receptor
  • 168 of 444 (37.8%) LPS-induced proteins were significantly reduced with the treatment of RGFP966, which mainly concentrated on Toll-like receptor signaling pathway. (hindawi.com)
  • Nuclear receptor-binding factor 2 (NRBF-2) (Comodulator of PPAR and RXR). (yellowcouch.org)
  • These effects may be mediated by the defective functioning of estrogen via the estrogen receptor, which contains a zinc finger protein. (wikipedia.org)
  • The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). (wikipedia.org)
  • It has been speculated that the domain may have ancestrally acted as a spacer to push the receptor beyond the extracellular matrix. (wikipedia.org)
  • e.g. a truncation of the receptor protein at residue number 660 leads to domains 3,4 and 5 of the EGF precursor domain being missing. (wikipedia.org)
  • This precludes the movement of the receptor from the ER to the Golgi, and leads to degradation of the receptor protein. (wikipedia.org)
  • ACBP is also known as diazepam binding inhibitor (DBI) or endozepine (EP) because of its ability to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. (wikipedia.org)
  • It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. (wikipedia.org)
  • fusion
  • For example, one would predict that mislocalization of the apical syntaxin 3 to the basolateral domain would allow the inappropriate fusion of apical transport vesicles with that domain and reduce the fidelity of polarized trafficking. (rupress.org)
  • 3. The fusion protein according to claim 1, wherein the non-cytotoxic protease is a clostridial neurotoxin L-chain or an IgA protease. (patentsencyclopedia.com)
  • 4. The fusion protein according claim 1, wherein the translocation domain is the H N domain of a clostridial neurotoxin. (patentsencyclopedia.com)
  • 5. The fusion protein according to claim 2, wherein the protease cleavage site is a protease cleavage site that is cleaved by a protease selected from Enterokinase, Factor Xa, Tobacco Etch virus (TEV), Thrombin or PreScission. (patentsencyclopedia.com)
  • This chimeric RNA can then be translated into a fusion protein. (wikipedia.org)
  • Fusion proteins are very tissue-specific and they are frequently associated with cancers such as colorectal, prostate, and mesotheliomas. (wikipedia.org)
  • regulate
  • The structure, function and molecular biology of Ca2+ dependent regulatory proteins (particularly calmodulin) and the target proteins which they regulate is under study. (uky.edu)
  • variants
  • This disease displays autosomal dominant inheritance, and it is associated with a large number of variants in both sarcomeric and non-sarcomeric proteins. (frontiersin.org)
  • At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4. (wikipedia.org)
  • this transcript contains an alternate open reading frame (ARF) that specifies a protein that is structurally unrelated to the products of the other variants. (wikipedia.org)
  • interact
  • NPs may interact with these proteins in a similar way, and the interaction may lead to beneficial outcomes in vaccine delivery. (scirp.org)
  • The ARF product functions as a stabilizer of the tumor suppressor protein p53, as it can interact with and sequester MDM2, a protein responsible for the degradation of p53. (wikipedia.org)
  • pathway
  • The complement or mannose binding pathway, a part of the pathways in the innate immune systems, is one of the first lines of defense for the host cells that the NPs drug delivery system would encounter. (scirp.org)
  • complexes
  • PRC1 and PRC2 are two protein complexes that modify the expression of p16 through the interaction of various transcription factors that execute methylation patterns that can repress transcription of p16. (wikipedia.org)
  • promoter
  • Regulation of p16 is complex and involves the interaction of several transcription factors, as well as several proteins involved in epigenetic modification through methylation and repression of the promoter region. (wikipedia.org)
  • Transcription Factors
  • Like most transcription factors, the HIF transcription factor is responsible for the expression of a protein. (wikipedia.org)
  • p16 acts as a tumor suppressor by binding to CDK4/6 and preventing its interaction with cyclin D. This interaction ultimately inhibits the downstream activities of transcription factors, such as E2F1, and arrests cell proliferation. (wikipedia.org)
  • novel
  • Biochemical and biotechnological development has allowed novel approaches to this issue, in the form of engineered O2 carriers, widely known as "blood substitutes. (wikipedia.org)
  • conformational
  • On the other hand the fluorescence emission of the protein increased upon binding to the PLGA-PEG-NH2 indicating conformational changes in the protein structure. (scirp.org)
  • It is thought that this region is responsible for the pH-dependent conformational shift that causes bound LDL to be released in the endosome. (wikipedia.org)