• Phase-separation was observed in the form of domains made of dipalmitoylphosphatidylcholine (DPPC), sphingomyelin (SM), or SM/cholesterol (SM/Chl) surrounded by a fluid matrix of dioleoylphosphatidylcholine (DOPC). (ac.be)
  • Time-lapse images collected following addition of 1 mM azithromycin revealed progressive erosion and disappearance of DPPC gel domains within 60 min. (ac.be)
  • The domains increased in average size from 100 μ² to 1500 μ² in a nonlinear manner as a function of area occupied per molecule of the DPPC, when monolayers were compressed at a rate of 20 mm²/sec. (mun.ca)
  • In this work, the formation of aqueous lipid/nanoparticle assemblies (LNAs) consisting of hydrophobic silver-decanethiol particles (5.7 ± 1.8 nm) embedded within 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers is demonstrated as a function of the DPPC/Ag nanoparticle (AgNP) ratio. (biomedcentral.com)
  • BPIFA1/SPLUNC1 binds with high affinity and specificity to dipalmitoylphosphatidylcholine, one of the major and most important surfactant phospholipids in the airway and lungs. (wikipedia.org)
  • Among the four surfactant apoproteins identified, surfactant protein B (SP-B) and SP-C are two small hydrophobic proteins that make up 2-4% of the surfactant mass and are present in commercially available surfactant preparations. (medscape.com)
  • During the last decades, the approach to overcome the complexity of biological membranes is to use simplified biomimetic models mainly consisting of binary or ternary mixtures of lipids [ 1 , 2 ]. (hindawi.com)
  • No domain formation was observed in monolayers for lipids with one or two unsaturated chains. (mun.ca)
  • Likewise, Jang et al [ 7 ] embedded 2.5-3.5 nm silicon particles with chemisorbed 1-octanol into bilayer membranes composed of DOXYL-labeled phosphocholine lipids. (biomedcentral.com)
  • Here we used real-time atomic force microscopy (AFM) imaging to visualize the interaction of the antibiotic azithromycin with lipid domains in model biomembranes. (ac.be)
  • This work provides direct evidence that the perturbation of lipid domains by azithromycin strongly depends on the lipid nature and opens the door for developing new applications in membrane biophysics and pharmacology. (ac.be)
  • Lipid rafts or ordered lipid domains may play a role in the localization, transport, and function of different proteins. (hindawi.com)
  • The caerin 1 peptides are among the most powerful of the broad-spectrum antibiotic amphibian peptides [PMID: 12717721]. (bicnirrh.res.in)
  • N-terminal Early set domain associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (also called Glycosyltrehalose trehalohydrolase) and similar proteins. (unl.edu)
  • E or "early" set domains are associated with the catalytic domain of Maltooligosyl trehalose trehalohydrolase (MTHase) and similar proteins at the N-terminal end. (unl.edu)
  • Early set domain associated with the catalytic domain of pullulanase-like proteins. (unl.edu)
  • A common feature of all EGF-like domains is that they are found in the extracellular domain of membrane-bound proteins or in proteins known to be secreted (exception: prostaglandin G/H synthase). (embl.de)
  • There are 539475 EGF domains in 145300 proteins in SMART's nrdb database. (embl.de)
  • Taxonomic distribution of proteins containing EGF domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with EGF domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing EGF domain in the selected taxonomic class. (embl.de)
  • Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. (embl.de)
  • The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains. (embl.de)
  • EGF-like domains in extracellular matrix proteins: localized signals for growth and differentiation? (embl.de)
  • Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. (embl.de)
  • This family consists of several caerin 1 proteins from Litoria species, Australian tree frogs. (bicnirrh.res.in)
  • Structurally, these proteins consist of three domains: a signal sequence, a conserved region of about 100 residues that contains four cysteines involved in two disulphide bonds, and a highly divergent C-terminal section of variable size. (bicnirrh.res.in)
  • It first binds with high affinity to specific cell-surface receptors and then induces their dimerization, which is essential for activating the tyrosine kinase in the receptor cytoplasmic domain, initiating a signal transduction that results in DNA synthesis and cell proliferation. (embl.de)
  • As a matter of fact, the number of studies on the influence of domain partitioning on interactions with peptides and nucleic acids has increased significantly in recent years [ 4 - 7 ]. (hindawi.com)
  • The N-terminal domain of MTHase may be related to the immunoglobulin and/or fibronectin type III superfamilies. (unl.edu)
  • The DEC-205 monomer forms a compact structure comprising two intercalated rings of C-type lectin-like domains, where the N-terminal cysteine-rich and fibronectin domains reside at the central intersection. (uci.edu)
  • The shapes of the domains were found to change dramatically when a small amount (2 mol %) of cholesterol was incorporated in the monolayer. (mun.ca)
  • Exposure of cells to either octanoic acid (C8:0), a C8-ceramide hydrolysis product, or oleic acid (C18:1) did not affect MDR1 expression. (sphingolipidclub.com)
  • BPI fold containing family A, member 1 (BPIFA1), also known as Palate, lung, and nasal epithelium clone (PLUNC), is a protein that in humans is encoded by the BPIFA1 gene. (wikipedia.org)
  • Secondary outcomes included lung function (forced expiratory volume at one second (FEV 1 )), P aeruginosa density in sputum, and the Cystic Fibrosis Quality of Life Questionnaire-Revised (CFQ-R). (bmj.com)
  • Inferring functional information from domain co-evolution. (auth.gr)
  • Gonzalez A., Duran Sepulveda L., Araya-Secchi R., Garate J. A., Pessoa-Mahana C. D., Lagos C. F. and Perez-Acle T. Computational modeling study of functional microdomains in cannabinoid receptor type 1. (cienciavida.org)
  • These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. (unl.edu)
  • The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). (unl.edu)
  • BPIFA1 is a member of a BPI fold protein superfamily defined by the presence of the bactericidal/permeability-increasing protein fold (BPI fold) which is formed by two similar domains in a "boomerang" shape. (wikipedia.org)
  • This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. (unl.edu)
  • The SP-B gene is on human chromosome 2, and its primary translation product is 40 kd, which is clipped to become an 8-kd protein in the type II cells before entering lamellar bodies to be cosecreted with phospholipids. (medscape.com)
  • The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions. (embl.de)
  • The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. (embl.de)
  • FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
  • Acute exposure ofMCF-7 and MDA-MB-231 cells to C8-glucosylceramide (10 μg/ml culture medium), a cell-permeable analog of glucosylceramide, increased MDR1 mRNA levels by 2- and 4-fold, respectively. (sphingolipidclub.com)
  • We suggest that the higher membrane stability of SM-containing domains results from stronger intermolecular interactions between SM molecules. (ac.be)
  • These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. (unl.edu)
  • Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
  • Unite de chimie des interfaces, Universite catholique de Louvain, Croix du Sud 2/18, B-1348 Louvain-la-Neuve, Belgium. (ac.be)
  • Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)
  • The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. (embl.de)
  • We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. (embl.de)
  • The resultant images are captured on conventional high resolution photographic film [1] , digitized using a standard flatbed scanner, and then subjected to computer processing using a standard PC running MS Windows to yield a 3-dimensional image which can be further manipulated, based on available software and the investigators' objectives. (chronopause.com)
  • The size, shape and growth of these domains were quantitatively characterized and found to be dependent on compression rates. (mun.ca)
  • the E-value for the EGF domain shown below is 1.07e-5. (embl.de)
  • The EGF-like domain includes six cysteine residues which have been shown to be involved in disulfide bonds. (embl.de)
  • the E-value for the EGF domain shown below is 6.25e-7. (embl.de)
  • An illustration of a vesicle assembly is shown in Figure 1 (not to scale). (biomedcentral.com)
  • [8] The process of USSTEM is shown in Figure 1 . (chronopause.com)
  • Although acetoxy derivatives of the 3,13- and 2,13-dienes showed almost the same mass spectra, the alcohols were distinguished by comparing the relative intensities of [M−18] + at m ⁄ z 248, indicating direct differentiation of the two positional isomers without derivatization. (go.jp)
  • The phase changes were observed by incorporating 1 mol% of a fluorescent probe, NBD-PC, into the lipid forming the monolayer. (mun.ca)
  • The liquid - expanded to liquid - condensed phase transition was visualized as the formation of dark patches (domains) from a fluorescent background. (mun.ca)
  • The serial images obtained using TEM are aligned and stacked, at which point software is used to render a 3-dimensional representation of the imaged tissue ( Figure 2 and Figure 19 ). (chronopause.com)
  • Influence of Sphingolipids on Expression of the Multidrug Resistance Phenotype1,2. (sphingolipidclub.com)
  • This subfamily also includes bacterial alpha amylases and 1,4-alpha-glucan branching enzymes which are highly similar to MTHase. (unl.edu)
  • Epidermal growth factor-like domain. (embl.de)
  • At a slow compression speed (0.13 A²/molecule/sec) the domains were larger in size and more regular in shape. (mun.ca)
  • MTSase is responsible for converting the alpha-1,4-glucosidic linkage to an alpha,alpha-1,1-glucosidic linkage at the reducing end of the maltooligosaccharide through an intramolecular transglucosylation reaction, while MTHase hydrolyzes the penultimate alpha-1,4 linkage of the reducing end, resulting in the release of trehalose. (unl.edu)