Chemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and Equipment
DolicholDolichol PhosphatesDiterpenesMevalonic AcidTerpenesFarnesolAlcoholsPentanesDolichol Monophosphate MannosePolyisoprenyl PhosphatesFatty AlcoholsChromatography, Thin LayerAlkyl and Aryl TransferasesUbiquinoneChromatography, High Pressure Liquid
Mannose-P-dolichol utilization defectOligosaccharidePhosphateGlycosylationKinaseLipidsProteinsMonophosphatePolyprenolsSynthesisStatinsIsoprenoidCarrierBacterialReductaseLiverFormPrecursorSubstratesEnzymesDonorPolyprenolDeficiencyGlcNAcEukaryotesMetabolismMonosaccharidesLipidUbiquinoneCellsIncreaseShows
Mannose-P-dolichol utilization defect1
  • Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins. (embl.de)
Oligosaccharide7
  • Dolichols function as a membrane anchor for the formation of the oligosaccharide Glc3-Man9-GlcNAc2 (where Glc is glucose, Man is mannose, and GlcNAc is N-acetylglucosamine). (wikipedia.org)
  • This oligosaccharide is transferred from the dolichol donor onto certain asparagine residues (onto a specific sequence that is "Asn-X-Ser/Thr") of newly forming polypeptide chains. (wikipedia.org)
  • During glycosylation, sugars are added to dolichol phosphate in order to build the oligosaccharide chain. (medlineplus.gov)
  • Once the chain is formed, dolichol phosphate transports the oligosaccharide to the protein that needs to be glycosylated and attaches it to a specific site on the protein. (medlineplus.gov)
  • The oligosaccharide donor for the enzyme is the dolichol pyrophosphate linked oligosaccharide GlcNAc 2 Man 9 Glc 3 . (umassmed.edu)
  • In eukaryotes, the synthesis of the oligosaccharide core is mediated by a lipid carrier called dolichol-phosphate (Dol-P) which is located in the membranes of the endoplasmic reticulum (ER, Fig. 1a ). (biomedcentral.com)
  • In addition to mature glycan structure determination, the Spiro lab also focused on studies of dolichol-oligosaccharide precursor biosynthesis, transfer to nascent polypeptides via oligosaccharyltransferase, and early glycan trimming events in the ER as a basis for studies on ER glucosidases, exo-mannosidases, and the first identification and characterization of an endo-mannosidase involved in glycan maturation. (glycobiology.org)
Phosphate17
  • Dolichols play a role in the co-translational modification of proteins known as N-glycosylation in the form of dolichol phosphate. (wikipedia.org)
  • Dolichol phosphate was discovered at the University of Liverpool in the 1960s, although researchers did not know its function at the time of discovery. (wikipedia.org)
  • During aging, the human brain shows a progressive increase in levels of dolichol, a reduction in levels of ubiquinone, but relatively unchanged concentrations of cholesterol and dolichyl phosphate. (wikipedia.org)
  • Scholars@Duke publication: A predicted geranylgeranyl reductase reduces the ω-position isoprene of dolichol phosphate in the halophilic archaeon, Haloferax volcanii. (duke.edu)
  • In N-glycosylation in both Eukarya and Archaea, N-linked oligosaccharides are assembled on dolichol phosphate prior to transfer of the glycan to the protein target. (duke.edu)
  • However, whereas only the α-position isoprene subunit is saturated in eukaryal dolichol phosphate, both the α- and ω-position isoprene subunits are reduced in the archaeal lipid. (duke.edu)
  • The agents responsible for dolichol phosphate saturation remain largely unknown. (duke.edu)
  • The present study sought to identify dolichol phosphate reductases in the halophilic archaeon, Haloferax volcanii. (duke.edu)
  • Likewise, the absence of HVO_1799 led to a loss of saturation of the ω-position isoprene subunit of C(55) and C(60) dolichol phosphate, with the effect of HVO_1799 deletion being more pronounced with C(60) dolichol phosphate than with C(55) dolichol phosphate. (duke.edu)
  • Glycosylation of dolichol phosphate in the deletion strain occurred preferentially on that version of the lipid saturated at both the α- and ω-position isoprene subunits. (duke.edu)
  • The synthesis in mouse testes of the isoprenoid lipid dolichol, the phosphate esters of which are involved in glycoprotein assembly, was investigated. (jax.org)
  • This gene provides instructions for making the enzyme dolichol kinase, which facilitates the final step of the production of a compound called dolichol phosphate. (medlineplus.gov)
  • Without properly functioning dolichol kinase, dolichol phosphate is not produced and glycosylation cannot proceed normally. (medlineplus.gov)
  • We have isolated a portion of the uridine diphosphate N-acetyl-D-glucosamine:dolichol phosphate N-acetyl-glucosamine-1-phosphate transferase gene (GTR2) from the genome of a tunicamycin-resistant clonal Chinese hamster ovary cell line, 3E11. (johnshopkins.edu)
  • Scocca, JR & Krag, SS 1990, ' Sequence of a cDNA that specifies the uridine diphosphate N-acetyl-D-glucosamine:dolichol phosphate N-acetylglycosamine-1-phosphate transferase from Chinese hamster ovary cells ', Journal of Biological Chemistry , vol. 265, no. 33, pp. 20621-20626. (johnshopkins.edu)
  • Tunicamycin blocks the formation of protein N-glycosidic linkages by inhibiting the transfer of N-acetylglucosamine 1-phosphate to dolichol monophosphate. (sigmaaldrich.com)
  • Phosphomannomutase 2 is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. (prospecbio.com)
Glycosylation1
  • Moreover, because of the requirements and spin-offs of this project, new significant research objectives were identified and targeted, such as the structural characterisation and utilisation of the dolichol-phospho- beta -d-mannose synthase, which was a key enzyme for human and pathogenic glycosylation, and the elucidation of the d-mannose function in teicoplanin antibiotic action. (europa.eu)
Kinase2
  • Mutations in the DOLK gene lead to the production of abnormal dolichol kinase with reduced or absent activity. (medlineplus.gov)
  • Helander A, Stodberg T, Jaeken J, Matthijs G, Eriksson M, Eggertsen G. Dolichol kinase deficiency (DOLK-CDG) with a purely neurological presentation caused by a novel mutation. (medlineplus.gov)
Lipids1
  • Because the pathways for dolichol and cholesterol branch from a common sequence of reactions, comparisons of the rates of synthesis of these two lipids can reveal specific alterations in the flow of metabolites along the branch leading to dolichol. (jax.org)
Proteins1
  • In addition, dolichols can be adducted to proteins as a posttranslational modification, a process in which branched carbohydrate trees are formed on a dolichol moiety and then transferred to an assembly of proteins to form a large glycoprotein in the rough endoplasmic reticulum. (wikipedia.org)
Monophosphate1
  • Dolichol monophosphate glucose: an intermediate in glucose transfer in liver. (neglectedscience.com)
Polyprenols3
  • Dolichols are found in eukaryotes and in archaea, although similar polyprenols molecules are found in bacteria. (wikipedia.org)
  • The Australian pharmaceutical company Solagran has been investigating the medical significance of polyprenols and their substitution with dolichols when ingested where there is a deficiency. (wikipedia.org)
  • A total of 92 neutral compounds including triterpenoids, polyprenols, and dolichols were identified using GC-MS and HPLC methods. (researchgate.net)
Synthesis4
  • Elevated dolichol synthesis in mouse testes during spermatogenesis. (jax.org)
  • Our results showed that the ratio of dolichol to sterol synthesis in testes was from 20- to 72-fold higher than any ratios we have measured in murine liver or cell cultures. (jax.org)
  • Thus, the high rate of dolichol synthesis in normal testes appeared to be associated with one or more of the spermatogenic cell types. (jax.org)
  • Lec15 and Lec35 are recessive Chinese hamster ovary (CHO) cellglycosylation mutations characterized by inefficient synthesis andutilization, respectively, of mannose-P-dolichol (MPD). (embl.de)
Statins3
  • Statins decrease dolichol levels in the body. (wikipedia.org)
  • For neuropathy caused by statins, there is new research on the use of dolichols that looks encouraging. (earthclinic.com)
  • Besides blocking cholesterol production, the statins block production of coenzyme Q10, which has been associated with cardiac benefit and dolichol, which has associated with cardiac function. (medpagetoday.com)
Isoprenoid2
  • Dolichol refers to any of a group of long-chain mostly unsaturated organic compounds that are made up of varying numbers of isoprene units terminating in an α-saturated isoprenoid group, containing an alcohol functional group. (wikipedia.org)
  • Lastly, the α isoprenoid unit is saturated by α-saturase (still a hypothetical enzyme), and this α-saturated polyprenyl alcohol is known as dolichol. (wikipedia.org)
Carrier1
  • Dolichol is also involved in transfer of the monosaccharides to the forming Glc3-Man9-GlcNAc2-Dolichol carrier. (wikipedia.org)
Bacterial1
  • For these reasons we chose to prepare them biosynthetically, through exploitation of the action of another recently identified bacterial glycosyltrasferase known as dolichol-phospho-beta-d-mannose synthase (DPMS). (europa.eu)
Reductase1
  • Dolichol is a product of the HMG-CoA reductase pathway (also known as the mevalonate pathway), and as such their creation and availability are affected by mevalonate inhibition. (wikipedia.org)
Liver1
  • It was also observed that newly biosynthesized testicular dolichols are slightly more polar than the pig liver dolichols used as standards. (jax.org)
Form1
Precursor1
  • Dolichols were first described about 30 years ago when animal tissues were being examined for the presence of a putative precursor to the polyisoprenoid side chain of ubiquinone. (nih.gov)
Substrates2
  • they include defects in synthetic pathways for N-linked oligosaccharides, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols. (nih.gov)
  • Within the ER (in the lumen), activated dolichol-bound sugars are used as donor substrates instead of nucleotide sugars for glycosyltransferases that synthesize N-glycans. (kth.se)
Enzymes1
  • These enzymes use dolichol-P-mannose as the sugar donor. (cazy.org)
Donor1
  • This oligosaccharide is transferred from the dolichol donor onto certain asparagine residues (onto a specific sequence that is "Asn-X-Ser/Thr") of newly forming polypeptide chains. (wikipedia.org)
Polyprenol2
  • CHB11-1-3 was found to be in the Lec9 complementation group, which synthesizes polyprenol rather than dolichol. (nih.gov)
  • Man5GlcNAc2-P-P-polyprenol and Man5GlcNAc5-P-P-dolichol both appeared to be translocated efficiently in an in vitro reaction. (nih.gov)
Deficiency2
  • The Australian pharmaceutical company Solagran has been investigating the medical significance of polyprenols and their substitution with dolichols when ingested where there is a deficiency. (wikipedia.org)
  • Blocking dolichols can lead to your cells responding chaotically because of dolichol deficiency. (redolaughlin.com)
GlcNAc2
  • Dolichols function as a membrane anchor for the formation of the oligosaccharide Glc3-Man9-GlcNAc2 (where Glc is glucose, Man is mannose, and GlcNAc is N-acetylglucosamine). (wikipedia.org)
  • 9. The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing. (nih.gov)
Eukaryotes1
  • Dolichols are found in eukaryotes and in archaea, although similar polyprenols molecules are found in bacteria. (wikipedia.org)
Metabolism3
Monosaccharides1
  • Dolichol is also involved in transfer of the monosaccharides to the forming Glc3-Man9-GlcNAc2-Dolichol carrier. (wikipedia.org)
Lipid1
  • Dolichols are the major lipid component (14% by mass) of human substantia nigra (SN) neuromelanin. (wikipedia.org)
Ubiquinone2
  • In the neurodegenerative disease Alzheimer's disease, the situation is reversed, with decreased levels of dolichol and increased levels of ubiquinone. (wikipedia.org)
  • Administration of 2-(diethylhexyl)phthalate to control mice resulted in elevated ubiquinone levels in the liver, while dolichol, dolichyl-P and cholesterol concentrations remained unchanged. (psu.edu)
Cells1
  • The Man5GlcNAc2 oligosaccharide attached to prenol in CHB11-1-3 cells and to dolichol in parental cells is the same structure, as determined by acetolysis. (nih.gov)
Increase1
  • In the infantile form, there is storage of saposins A and D. There is an increase in dolichols in the urine and brain of the patients. (nih.gov)
Shows1