• An Epstein-Barr viral gene (ZEBRA) is identified that, in human lymphoblastoid cells, activates a switch causing the virus to shift from the latent to the replicative phase of its life cycle. (pnas.org)
  • We have shown that a 2.7-kilobase-pair rearranged Epstein-Barr virus DNA fragment of this gene (BamHI fragment WZhet) induced transient expression of viral replicative antigens and polypeptides when it was transfected into a somatic cell hybrid, which was derived from the fusion of an epithelial line cell with a Burkitt lymphoma cell. (pnas.org)
  • The structure of intracellular viral DNA from a number of cell lines arising by clonal transformation of human lymphocytes in vitro with Epstein-Barr virus was analyzed. (asm.org)
  • We have not detected in Epstein-Barr viral plasmids analogs of the gross rearrangements of viral DNAs observed after lytic infections with high multiplicities of papova-, adeno-, or herpes simplex viruses. (asm.org)
  • VP16 from the virion tegument forms a complex with HCF-1 and Oct1 that binds to viral IE promoters and HCF-1 recruits histone modification enzymes and chromatin remodeling complexes that decrease histone association with viral IE genes and increase euchromatin marks on the remaining associated histones. (nih.gov)
  • The multimerized IFI16 on the viral DNA also recruits histone modification complexes that add H3K9me3 modifications to the viral chromatin, resulting in epigenetic silencing of the viral genes. (nih.gov)
  • It has been suggested to mediate protein-DNA and protein-protein interactions during chromatin organization and transcription. (wikipedia.org)
  • On episode #106 of the podcast This Week in Virology , Vincent, Dickson, and Rich continue Virology 101 with a second installment of their discussion of how viruses with DNA genomes replicate their genetic information. (virology.ws)
  • The viral DNA rapidly circularizes, and nuclear IFI16 binds to the viral DNA and multimerizes, initiating a nuclear-to-cytoplasmic signaling cascade that activates IRF-3, which dimerizes and translocates to the nucleus. (nih.gov)
  • Whereas conventional surface hybridization detection uses only a single probe, the method described by Lee and his colleagues, Jiangwei Li and Edward S. Yeung, incorporates a second probe, and the target DNA is sandwiched between this probe and the surface. (photonics.com)
  • In general, a strong association was observed between two different viral DNA/RNA extraction kits and detection frequency of targets ( P = 0.017). (iwaponline.com)
  • To determine the mechanism of this packaging motor, we used a novel high-resolution optical tweezers to observe the discrete increments of DNA packaged by a single bacteriophage. (aps.org)
  • The viral fragments were detected by transferring the DNA from the gel to nitrocellulose sheets, hybridizing radiolabeled recombinant vectors carrying fragments of viral DNA to those transfers, and visualizing the hybrids by autoradiography. (asm.org)
  • With a featured publication in the Aug. 7 issue of Science, Montana State University researchers have made a significant contribution to the understanding of a new field of DNA research, with the acronym CRISPR, that holds enormous promise for fighting infectious diseases and genetic disorders. (infectioncontroltoday.com)
  • VP16 cannot be transported into the neuronal nucleus, and HCF-1 is not localized in the nucleus so viral IE genes are not transcribed efficiently. (nih.gov)
  • The panels here represent samples with 0 (A), 10 -4 (B), 10 -3 (C), 10 -2 (D), 10 (E) and 1 percent human papilloma virus DNA. (photonics.com)
  • Purifies viral nucleic acids from human and animal samples, including plasma, serum, saliva, and urine, using magnetic bead technology amenable to high-throughput operations. (fishersci.com)
  • The Thermo Scientific MagJET Viral DNA and RNA Kit is designed for fast and efficient purification of viral nucleic acids from various human and animal liquid samples such as plasma, serum, saliva and urine, as well as from nasal, buccal and urogenital swabs and blood. (fishersci.com)
  • Nucleic acid was isolated from 200 µL of human whole blood with Omega Bio-tek's Mag-Bind® Viral DNA/RNA Kit and a comparable kit from Company A using the manufacturer's recommended protocols. (omegabiotek.com)
  • In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes. (bmj.com)
  • DNA copy number analysis was done on pure tumor cell samples enriched with flow sorting. (eurekamag.com)
  • Therefore, the researchers used Alexa Fluor 532 covalently bound to a DNA fragment. (photonics.com)
  • In order to test the ability of these tools to detect viral specific reads in NGS data from tumors, data obtained from the HPV16 positive cervical squamous cell carcinoma cell line SiHa were analyzed. (aacrjournals.org)
  • Elde's study suggests that our bodies' appropriation of viral DNA has been critically important to our survival as a species. (pewtrusts.org)
  • Mag-Bind® Viral DNA/RNA Kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from whole blood, serum, plasma, saliva and other body fluids. (omegabiotek.com)
  • In this study, two commercially available kits were compared to assess their performance, the MO BIO PowerViral Environmental DNA/RNA Isolation kit and the Qiagen QIAamp Viral RNA Mini kit. (iwaponline.com)
  • The structure of this biological machine is conceptually similar to an engineer's blueprint, and it explains how each of the parts in this complex assemble into a functional complex that efficiently identifies viral DNA when it enters the cell," Wiedenheft says. (infectioncontroltoday.com)
  • We propose to test our primary hypothesis that treatment with Peg-IFN-α-2b will result in a decrease in integrated HIV DNA in peripheral blood and tissue in chronically HIV-infected immune-reconstituted individuals (see section 3.1) in a prospective, interventional, 1-arm, open label clinical trial. (clinicaltrials.gov)
  • It was particularly surprising that AAV failed to elicit immune responses to highly expressed neoantigenic transgene products when injected into muscle ( 11 , 23 , 45 ) whereas other vector systems expressing the identical transgene, such as adenovirus ( 52 ) and naked DNA, do. (asm.org)
  • Our results highlight the value of integrative proteomics in deducing protein function and establish IFIX as an antiviral DNA sensor important for mounting immune responses. (sigmaaldrich.com)
  • Here, we describe the quantitative relation between HPV DNA copy number and mRNA expression of the viral oncogenes (E6 and E7) and the prognostic value of both measures in cervical cancer patients. (eurekamag.com)
  • Activation of the double hexamer on origin DNA requires a unique phosphorylation state of Tag: phospho-Thr124, and unmodified Ser120 and 123 , . (prolekare.cz)
  • Homomeric ring-ATPases of the ASCE family are responsible for a variety of important cellular functions, ranging from DNA translocation to protein degradation. (aps.org)
  • IFI16 also plays a role in the heterochromatinization of HSV DNA, and this review will examine how IFI16 integrates epigenetic regulation and innate sensing of foreign viral DNA to show how these two responses are related. (nih.gov)
  • They knew that, under the conditions that they used, DNA would coil randomly on the slide surface after hybridization, leading to less shearing and more robust performance of the tag throughout the hybridization step. (photonics.com)
  • The cellular sensors that initiate the chromatinization of foreign DNA have not been fully defined. (nih.gov)