Dna sequence dna catalytic. Medical search. Frequent questions

Sequence-specific cleavage of Huntington mRNA by catalytic DNA. (bcm.edu)
Here we use single-molecule and bulk biochemical experiments to determine how Cas9-RNA interrogates DNA to find specific cleavage sites. (nature.com)
We show that both binding and cleavage of DNA by Cas9-RNA require recognition of a short trinucleotide protospacer adjacent motif (PAM). (nature.com)
Distinct sequence and spacing requirements of the catalytic domain result in reduced cleavage activity on operator DNA. (rcsb.org)
Inteins possess two different enzymatic activities, self-catalyzed protein splicing and site-specific DNA cleavage. (rcsb.org)
A docking model of the DNA-enzyme complex suggests that the endonuclease domain covers the 20 bp DNA duplex encompassing the cleavage site, whereas the Stirrup domain could make an additional contact with another upstream 10 bp region. (rcsb.org)
The presence of uranyl causes catalytic cleavage of the DNA and release of the fluorophore, resulting in a dramatic increase of fluorescence intensity. (nanowerk.com)
In short, they are able to introduce transient cleavage of both strands of the DNA double helix, thereby allowing the passage of another intact DNA double strand through the cleavage. (allindianpatents.com)
The topo II poisons shift the equilibrium of the catalytic cycle towards cleavage, thereby increasing the concentration of the transient protein-associated breaks in the genome (1) (see Fig.1). (allindianpatents.com)
We also show that SpCas9-mediated cleavage is unaffected by DNA methylation and that the dosage of SpCas9 and sgRNA can be titrated to minimize off-target modification. (cdc.gov)
Although an sgRNA design consisting of a truncated crRNA and tracrRNA had been previously shown to mediate efficient cleavage in vitro 12 , it failed to achieve detectable cleavage at several loci that were efficiently modified by crRNA-tracrRNA duplexes bearing identical guide sequences 1 . (cdc.gov)
sgRNAs with +67 or +85 nucleotide (nt) tracrRNA tails mediated DNA cleavage at all target sites tested, with up to fivefold higher levels of indels than the corresponding crRNA-tracrRNA duplexes ( Fig. 1b and Supplementary Fig. 1a ). (cdc.gov)
We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

Cyclin-dependent kinases (CDKs) are key regulatory enzymes, each consisting of a catalytic CDK subunit and an activating cyclin subunit. (kegg.jp)
It would appear that the molecular system because of this up-regulation functions partly through important Ras binding, mimicking the p110-helical website mutants [16] and perhaps through stabilization from the catalytic subunit [17]. (cell-signaling-pathways.com)
Nevertheless, it did create a significant upsurge in the autophosphorylation from the catalytic subunit in oncogenic types of p110 and elevation of autophosphorylation of most wt (wild-type) isoforms. (cell-signaling-pathways.com)
Components AND Strategies Recombinant kinase synthesis All course 1a isoforms and mutants had been created in-house by co-expressing full-length human being p85 using the indicated human being full-length catalytic subunit. (cell-signaling-pathways.com)
nevertheless, just the catalytic p110 subunit was indicated (p110, "type":"entrez-nucleotide","attrs":"text message":"NM_002649″,"term_id":"539846528″,"term_text message":"NM_002649″NM_002649). (cell-signaling-pathways.com)
4. Measure telomerase activity in the tissues by quantitative immunhistochemical analysis of the catalytic subunit telomerase reverse transcritptase. (europa.eu)

Several retrons are located in DNA regions next to certain protein effector-coding genes. (wikipedia.org)
On germline analysis, they identified a rare variant affecting PRKDC , protein kinase DNA-activated catalytic polypeptide (DNA-PKcs). (medscape.com)
We present a model in which the flexibility in protein-DNA interactions allows I-TevI to bind variant intronless alleles to promote intron mobility while facilitating its function in autorepression, and thereby persistence in its host. (rcsb.org)
The horseshoe-shaped Hint domain contains a catalytic center for protein splicing, which involves both N and C-terminal residues. (rcsb.org)
In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 tumor suppressor protein. (kegg.jp)
During a processive reaction, the DNA strand (blue) anneals to the alignment region in the TR (pink), the catalytic TERT protein (grey) reverse transcribes the RNA template (red), adding 6 deoxyribonucleotides (blue) to the DNA strand. (asu.edu)
Preclinical data reported here show that BN80927 retains Topo I poisoning activity in cell-free assay (DNA relaxation) as well as in living cells, in which in vivo complexes of topoisomerase experiments and quantification of DNA-protein-complexes stabilization, have confirmed the higher potency of BN80927 as compared with the Topo I inhibitor SN38. (aacrjournals.org)
The cloning of maize PPDK regulatory protein (PDRP) revealed the regulatory protein had been sequenced from more than 200 bacterial species and had been identified as DUF299 (domain of unknown function). (scirp.org)
We previously showed that two inactive fragments of green fluorescent protein, each coupled to engineered zinc finger DNA-binding proteins, were able to reassemble an active reporter complex in the presence of a predefined DNA sequence. (nebraska.edu)
Because the ultimate function of the CAS protein is to cut DNA in the CRISPR-CAS9 system, and that's why it's there! (geneticeducation.co.in)
The native CAS9 protein is a nuclease that has the power to cut either double-strand or single-strand DNA. (geneticeducation.co.in)
dCAS9 is a specialized, artificially synthesized CAS protein that lacks one of the important properties which is catalytic activity. (geneticeducation.co.in)
So now after the CAS converting into the dCAS, it has been applied to deliver any functional domain or protein to a specific DNA location. (geneticeducation.co.in)
It is comprised of DNA wrapped around protein particles called histones. (poly.edu)
When the membrane is successfully disrupted, the DNA is released from the cells into the solution along with protein molecules and other cellular miscellanea. (poly.edu)
While most DNA is double stranded, the catalytic DNA Lu's research group uses has a single strand region that can wrap around like a protein. (nanowerk.com)
A simple transposon is organized by terminal repeat domains (TRDs) embracing a gene encoding a catalytic protein, transposase, required for its relocation in the genome through a "cut-and-paste" mechanism. (biomedcentral.com)
Structural studies of the Streptococcus pyogenes Cas9 showed that the protein exhibits a bilobed architecture comprising the catalytic nuclease lobe and the α-helical lobe of the enzyme and interactions between the two lobes seem to be mediated primarily through contacts with the bound nucleic acid rather than direct protein-protein contacts. (universityofcalifornia.edu)
In 1958, Crick's review "On Protein Synthesis" appeared: this laid out, for the first time, the central dogma of molecular biology , the sequence hypothesis and the adaptor hypothesis . (wikipedia.org)
instead, they combined RNA-DNA proximity ligation ( Red-C ) with chromatin immunoprecipitation (ChIP) to create RedChIP, which identifies RNAs associated with a DNA-bound protein of interest. (epigenie.com)
As a parallel to HiChIP , which takes a protein-centric view of mapping DNA-DNA interactions , RedChIP evaluates RNA-DNA interactions occurring with a given protein. (epigenie.com)
then, a specific antibody immunoprecipitates protein-associated RNA-DNA complexes. (epigenie.com)
Finally, RNA-DNA sequencing reports on RNA-DNA interactions mediated by the protein and protein-DNA interactions mediated by RNAs. (epigenie.com)
Indeed, the identification of Kcnq1ot1, which targets Polycomb complexes to repressed genomic domains, and the significant overlap of ncRNAs isolated via a conventional technique suggest that RedChIP represents the way forward for studies of RNA-protein-DNA interactions in live cells. (epigenie.com)

HotStarTaq DNA polymerase (Qiagen). (cdc.gov)
Simple and cost-effective, the selection process can sample a very large pool of DNA (up to 1,000 trillion molecules), amplify the desired sequence by the polymerase chain reaction, and introduce mutations to improve performance. (nanowerk.com)
Eukaryotic DNA polymerase β (pol β), the polymerase thought to be responsible for DNA repair synthesis, has been extensively characterized in rats and humans. (biomedcentral.com)
The former domain possesses a template-binding function and a dRPase activity to remove the 5'-deoxyribose phosphate, the latter domain possesses DNA polymerase activity. (biomedcentral.com)

2.0 fluorometer (Invitrogen, USA) and a Bioanalyzer (Agilent Technologies, USA), sequencing was performed on an Illumina MiSeq using a v. 3 reagent kit (Illumina), generating a dataset of 300 nucleotide paired-end reads. (cdc.gov)
They recognize long asymmetric nucleotide sequences and cleave both DNA strands in a monomer form. (rcsb.org)
These nanoscale applications often involve the synthesis of large sequences comprising thousands of the building blocks that DNA is made of, known as the A, T, C and G nucleotide bases, which can be further folded and structured due to the specific base-pairing abilities between As and Ts, and Cs and Gs, respectively. (nanowerk.com)
The TR component contains a template region that is complementary to the telomeric DNA sequence, while TERT contains the catalytic reverse transcriptase domain responsible for nucleotide addition. (asu.edu)
A variety of chemical modifications decorate the nucleic acids, increasing the alphabet of DNA to about a dozen known nucleotide variants. (pharmaceuticalintelligence.com)

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA-DNA base-pairing to target foreign DNA in bacteria. (nature.com)
Discovered by Carol Greider and Liz Blackburn in 1985 , telomerase is a unique ribonucleoprotein (RNP) enzyme that adds telomeric repeats to the 3' end of chromosomes to counteract the DNA degradation that occurs due to the end replication problem. (asu.edu)
DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two DNA fragments by catalysing the formation of an internucleotide ester bond between phosphate and deoxyribose. (embl.de)
With the exception of the atypically small PBCV-1 viral enzyme, two regions of primary sequence are common to all members of the family. (embl.de)
Here we endow the SEER system with catalytic capability using the reporter enzyme TEM-1 β-lacatamase. (nebraska.edu)
The catalytic domain of the enzyme is modified in such a way that it keeps intact its DNA binding abilities but doesn't execute cleaving. (geneticeducation.co.in)
A novel gene lipB, which encodes an extracellular lipolytic enzyme, was identified in the Bacillus subtilis genomic DNA sequence. (inra.fr)
The catalytic activities of these two COMT variants, expressed in E. coli, were similar, but the Met-108 enzyme was more thermolabile already at physiological temperature (37 °C). The reported existence of a common polymorphism of the human COMT gene coding for a thermolabile low activity, COMT(L), and a thermostable high activity, COMT(H), is discussed in light of the different thermostability of the two enzyme forms. (lu.se)
The antitumor effect is explained by the ability to inhibit the nuclear enzyme DNA topo II. (allindianpatents.com)

A retron is a distinct DNA sequence found in the genome of many bacteria species that codes for reverse transcriptase and a unique single-stranded DNA/RNA hybrid called multicopy single-stranded DNA (msDNA). (wikipedia.org)
Host sequences were removed by mapping the reads to the UCSC hg19 human reference genome. (cdc.gov)
The viral genome sequence was annotated by comparison with the KSHV and RFHVMn genome sequences (Geneious v. 11.1.3 ( 5 ). (cdc.gov)
The CbGHV1 genome sequence was deposited in NCBI GenBank (accession number MH932584). (cdc.gov)
SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. (cdc.gov)
Performing genome-wide target profiling on data sets obtained by retrieving chromosomal targeting sequences from individual clones, we have identified several piggyBac and Tol2 hotspots and observed that (4) piggyBac and Tol2 display a clear difference in targeting preferences in the human genome. (biomedcentral.com)
DNA transposons are natural genetic elements residing in the genome as repetitive sequences. (biomedcentral.com)
The CRISPR-Cas9 system can be used to quickly and specifically target and cleave DNA at sites defined by engineered single-guide RNAs (sgRNAs) and has led to its adoption as a robust and versatile platform for genome engineering. (universityofcalifornia.edu)
the m 6 A-containing fragments are then sequenced and aligned to the genome, thus allowing us to locate the positions of methylation marks. (pharmaceuticalintelligence.com)
While RNA-DNA proximity ligation techniques map genome-wide ncRNA interactions , they don´t describe the proteins involved. (epigenie.com)
To facilitate mammalian genome engineering applications, we provide a web-based software tool to guide the selection and validation of target sequences as well as off-target analyses. (cdc.gov)
The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. (lu.se)

Cytosine C5 methylation is an important epigenetic control mechanism in a wide array of eukaryotic organisms and generally carried out by proteins of the C-5 DNA methyltransferase family (DNMTs). (mdpi.com)
These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. (arigobio.com)
Surprisingly, a Dnmt1 mutant construct lacking the CXXC domain formed covalent complexes with cytosine bases both in vitro and in vivo and rescued DNA methylation patterns in dnmt1−/− embryonic stem cells (ESCs) just as efficiently as wild type Dnmt1. (cipsm.de)

These results reveal how Cas9 uses PAM recognition to quickly identify potential target sites while scanning large DNA molecules, and to regulate scission of double-stranded DNA. (nature.com)
Studies aimed at defining those elements necessary for the design of synthetic double strand DNA cleaving molecules with defined target sequences and binding site sizes are described. (caltech.edu)
The covalent modification of sequence specific DNA binding molecules with a chelated metal capable of redox chemistry generates bifunctional DNA cleaving molecules. (caltech.edu)
The research done in the Cravens lab is inspired by a desire to understand life at a fundamental level, primarily through biophysical studies of DNA and sequence-specific DNA targeting molecules. (gonzaga.edu)
The DNA will be extracted using the basic biochemical techniques for isolating, purifying, and digesting DNA molecules. (poly.edu)
The base sequence acts as the instruction manual of the cell, directing it on how to make proteins and other important molecules that an organism needs to survive and function. (poly.edu)
But these information molecules are much more than their core sequences. (pharmaceuticalintelligence.com)

Two recently published human COMT cDNA sequences differed in the position of S-COMT amino acid 108, the residue being either Val-108 [Lundstrom et al. (lu.se)
One family pedigree was described with X-linked ichthyosis associated with normal levels of STS and a normal molecular pattern, as detectable with a complementary DNA (cDNA) probe for the STS gene. (medscape.com)

The scope of studies ranges from those focused-on sequencing, mass spectrometry, bioimaging and other high-through-put techniques in data to medicine, pharmacology, and populational studies. (nih.gov)

Synthesis of DNA by the retron-encoded reverse transcriptase (RT) results in a DNA/RNA chimera which is composed of small single-stranded DNA linked to small single-stranded RNA. (wikipedia.org)
The DNA portion of msDNA is encoded by the msd gene, the RNA portion is encoded by the msr gene, while the product of the ret gene is a reverse transcriptase similar to the RTs produced by retroviruses and other types of retroelements. (wikipedia.org)

Mitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. (kegg.jp)
It is active during DNA replication, DNA repair and DNA recombination. (embl.de)
They compete with endogenous nucleotides (dNTP/NTP) for incorporation into nascent DNA/RNA and inhibit replication by preventing subsequent primer extension. (plos.org)
Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. (arigobio.com)
Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. (arigobio.com)
Pol γ "reads" sequences of mtDNA and uses them as templates to produce new copies of mtDNA in a process called DNA replication . (medlineplus.gov)

[ 10 ] Boland et al performed whole exome sequencing on germline DNA from blood samples and polyp tissue from a gastric and a colonic polyp. (medscape.com)

The ability of restriction endonucleases to cleave double helical DNA on opposite strands at or near 4-6 base pair recognition sequences makes possible DNA sequencing, gene isolation and recombinant DNA technology. (caltech.edu)
Fe(II) (10 -6 M) sequence specifically single strand cleave pBR-322 plasmid DNA (10 -5 bp) in the presence of O 2 and dithiothreitol (DTT). (caltech.edu)

The approach gives us tremendous creative freedom: we can not only synthesize the same piece of DNA again and again as new additions of a growing sequence, but we can also vary the types of DNA sequences to be appended simply by changing the composition of catalytic hairpin DNAs and primers in the mix while the assembly is ongoing. (nanowerk.com)

Amino acid sequence alignments were constructed for individual genes using Bioedit v. 7.2.0 or Geneious v. 11.1.3 ( 5 ) to calculate percentage identity. (cdc.gov)
Each of these pages lists information related to the component, the RNA or amino acid sequence, the genbank accession number, and a link to the record at the National Center for Biotechnology Information (NCBI) . (asu.edu)
The alignments page contains multiple RNA and amino acid sequence alignments based on both sequence and structural similarities. (asu.edu)
The amino acid located between the regulatory threonine and the catalytic histidine is highly conserved being serine in PPDK and cysteine in PEPS. (scirp.org)
A comparison of known PPDK and PEPS sequences revealed a high level of conservation of the amino acid sequence surrounding the catalytic histidine located within a central domain of the enzymes that was composed of GGXTS/CHAAI/VI/VA/SR with the regulatory threonine and catalytic histidine (shown in bold) conserved in all species. (scirp.org)
The catalytic triad of LipB consists of residues Ser78, Asp134, and His157 as demonstrated by amino-acid sequence alignments and site-directed mutagenesis. (inra.fr)

Using standard bioinformatics tools, searches were also conducted for potentially novel genes that had been missed in previous analyses of the KSHV and RFHVMn sequences. (cdc.gov)
A unique feature of our uranium sensor is that it contains a small piece of DNA, the same basic building blocks of our genes,' said Lu, who also is a researcher at the university's Beckman Institute for Advanced Science and Technology, and at the Center of Advanced Materials for the Purification of Water with Systems. (nanowerk.com)

May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. (arigobio.com)
That DNA binding capability of the dead CAS, scientists use to perform various functions like altering epigenetic activities, investigating chromatin structure, base editing, epigenetic remodeling and programming chromatin interactions. (geneticeducation.co.in)
The nucleus houses the DNA in the form of chromatin. (poly.edu)
Chromatin is the active form of DNA in the cell when it is not preparing for cell division. (poly.edu)
Several mammalian proteins involved in chromatin and DNA modification contain CXXC zinc finger domains. (cipsm.de)

Like other reverse transcriptases, the retron RT contains seven regions of conserved amino acids (labeled 1-7 in the figure), including a highly conserved tyr-ala-asp-asp (YADD) sequence associated with the catalytic core. (wikipedia.org)

In addition, BN80927 inhibits Topo II-mediated DNA relaxation in vitro but without cleavable-complex stabilization, thus indicating catalytic inhibition. (aacrjournals.org)

On the top, a 'catalytic PER hairpin' binds a first 'primer' (shown as a short grey strand), triggers its elongation with a sequence encoded by the hairpin itself, and releases it to start another cycle with the already extended primer, and so forth, until a long transcript is generated. (nanowerk.com)
Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. (arigobio.com)
In contrast, the CXXC domain of Dnmt1 selectively binds DNA substrates containing unmethylated CpG sites. (cipsm.de)

Non-target DNA binding affinity scales with PAM density, and sequences fully complementary to the guide RNA but lacking a nearby PAM are ignored by Cas9-RNA. (nature.com)
Cas9 contains two nuclease active sites that function together to generate DNA double-strand breaks (DSBs) at sites complementary to the guide RNA sequence and adjacent to a protospacer adjacent motif. (universityofcalifornia.edu)

For the double-strand break, the two strands in the DNA duplex were cleaved by PI-PfuI with different efficiencies. (rcsb.org)

This system, designated sequence-enabled reassembly (SEER), was demonstrated in vitro to produce a DNA-concentration-dependent signal. (nebraska.edu)
To search for the unique sequence of DNA that could distinguish uranyl from other metal ions, the researchers used a combinatorial approach called in vitro selection. (nanowerk.com)

The RNA strand is joined to the 5′ end of the DNA chain via a 2′-5′ phosphodiester linkage that occurs from the 2′ position of the conserved internal guanosine residue. (wikipedia.org)
Competition assays provide evidence that DNA strand separation and RNA-DNA heteroduplex formation initiate at the PAM and proceed directionally towards the distal end of the target sequence. (nature.com)
One is called a "catalytic DNA hairpin mediator", which is a single-stranded DNA molecule that partially pairs up with itself to form a hairpin structure with a short overhanging single strand. (nanowerk.com)
The telomerase reaction within vertebrates and ciliates is processive, iteratively adding hundreds of telomeric repeats onto the DNA strand. (asu.edu)

10976 in exon 8 in the catalytic region of ing factors that may increase cardiovascu- the FVII gene and an insertion of a decanu- lar disease. (who.int)

Through a series of elongation and displacement reactions, the primer is extended with a sequence provided by the catalytic hairpin mediator and then expelled. (nanowerk.com)
This frees up the catalytic hairpin mediator to cascade the next round of the process, either by capturing a new starting primer or the already elongated primer -- and so forth. (nanowerk.com)
This substitution lies in a hairpin sequence within an 8-kDa domain, likely to be important in DNA binding. (biomedcentral.com)

Recurrent chimeric RNAs enriched in human prostate cancer identified by deep-sequencing. (bcm.edu)
In addition, about 250 noncoding RNA sequences-including well-characterized long noncoding RNAs (lncRNAs), such as the XIST transcripts that have a key role in X-chromosome inactivation-are decorated by m 6 A. In almost all cases, the epigenetic mark was found on adenosines embedded in the predicted A/G-methylated A-C sequence. (pharmaceuticalintelligence.com)

It should be pointed out that although the two proteins (PPDK and PEPS) share some homology, especially around a central catalytic site, they can be identified using the signature sequences identified by Tjaden and colleagues [10]. (scirp.org)
This enabled the diversified complementarity and secondary structures that allow RNA species to specifically interact with other components of the cellular machinery such as DNA and proteins. (pharmaceuticalintelligence.com)
Now, "RedChIP" has us stampeding in anticipation towards the exploration of the complex interactions taking place between RNA, DNA, and proteins in the nucleus. (epigenie.com)

The sequences presented within the database were used for the construction of the alignments. (asu.edu)

The Wyss Institute's team used the new concept to design a series of such PER DNA transcripts for very diverse applications, including the autonomous synthesis of large DNA nanostructures known as DNA-origamis, and synthetic biology approaches, in which the synthesis of a DNA transcript hinges on a trigger, such as a cancer-associated small micro RNA. (nanowerk.com)

Moreover, the anthracyclines are capable of chelating iron and forming ternary complexes with DNA. (allindianpatents.com)

Furthermore, PAM interactions trigger Cas9 catalytic activity. (nature.com)
We modified the Dz13 DNAzyme (which showed promise of a therapeutic agent in preclinical studies by targeting the c-jun mRNA 14 ) and preprogrammed it into a library of Boolean logic gates where only upon specific conditions, Dz13 regains its catalytic activity. (nature.com)
Without nucleophilic catalytic activity, what is the use of CAS9? (geneticeducation.co.in)
Either mutation is designed for only preventing enzymatic activity but not DNA binding capability. (geneticeducation.co.in)
Four different lipase assays were used to determine its catalytic activity: pH-stat, spectrophotometry, fluorimetry and the monomolecular film technique. (inra.fr)
We show that the CXXC domain of Tet1 has no DNA binding activity and is dispensable for catalytic activity in vivo. (cipsm.de)
Because the major difference between this sgRNA design and the native crRNA-tracrRNA duplex is the length of the tracrRNA sequence, we tested whether extension of the tracrRNA tail would improve SpCas9 activity. (cdc.gov)

The sequences page is further broken down into separate pages for each of the components of telomerase. (asu.edu)

The cell wall is the first barrier in that must be broken to extract the DNA molecule inside the cell. (poly.edu)
The DNA molecule is a double-helical polymer consisting of a sugar-phosphate backbone with nitrogenous bases running perpendicular to the backbone. (poly.edu)

In particular, the invention relates to the use of a topo II catalytic inhibitor such as the bisdioxopiperazine ICRF-187 for the manufacture of a medicament for the treatment of an accidental extravasation of a topoisomerase II poison and a method for treatment of such extravasation of a topoisomerase poison such as the anthracyclines daunorubicin, doxorubicin, epirubicin, or idarubicin. (allindianpatents.com)
The present invention relates to a pharmaceutical composition for the prevention or treatment of tissue damage due to extravasation caused by a topoisomerase II poison in a patient receiving treatment with saved poisons and to the use of said topo II catalytic inhibitor for the manufacture of a medicament for the prevention or treatment of said tissue damage due to extravasation caused by said topoisomerase II poison. (allindianpatents.com)
Topoisomerase II, topoisomerase II poisons, and topoisomerase H catalytic inhibitors The topoisomerase II (topo II) enzymes belong to a system of nuclear enzymes involved in the processing of DNA during the cell cycles. (allindianpatents.com)

Published in Nature Chemistry ( 'Programmable autonomous synthesis of single-stranded DNA' ), research by Peng Yin at Harvard's Wyss Institute for Biologically Inspired Engineering provides a broadly applicable solution to this problem. (nanowerk.com)

Figure 1: DNA curtains assay for target binding by Cas9-RNA. (nature.com)
Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)

Following crRNA and tracrRNA hybridization, SpCas9 is targeted to genomic loci matching a 20-nt guide sequence within the crRNA, immediately upstream of a required 5′-NGG protospacer adjacent motif (PAM) 11 . (cdc.gov)

The topo II catalytic inhibitor is an entirely different group of drugs. (allindianpatents.com)

One is the inhibition of the initial binding of topo II to DNA as in the case of chloroquine (3) and aclarubicin (4,5). (allindianpatents.com)

The catalytic region comprises six conserved sequence motifs (I, III, IIIa, IV, V-VI), motif I includes the lysine residue that is adenylated in the first step of the ligation reaction. (embl.de)
Using antibodies, we demonstrated that the regulation of both Listeria monocytogenes PPDK and Escherichia coli PEP synthetase involves the phosphorylation of a threonine residue located close to the catalytic histidine residue. (scirp.org)

Since retrons are not mobile, their appearance in diverse bacterial species is not a "selfish DNA" phenomenon. (wikipedia.org)

Epigenetics broadly is a heritable genetic characteristic that is a cell-specific gene expression and regulation achieved by the interaction between DNA and transcriptional factors. (geneticeducation.co.in)
To fully explore the potential of two highly active DNA transposons, piggyBac and Tol2 , as mammalian genetic tools, we have conducted a side-by-side comparison of the two transposon systems in the same setting to evaluate their advantages and disadvantages for use in gene therapy and gene discovery. (biomedcentral.com)
Since the first discovery of DNA transposons in Maize by Barbara#McClintock in 1950 [ 1 ], transposons have been used extensively as genetic tools in invertebrates and in plants for transgenesis and insertional mutagenesis [ 2 - 7 ]. (biomedcentral.com)

After extension, the DNA dissociates from the RNA and the newly synthesized DNA (blue) anneals to alignment region in the TR (pink), ready for reverse transcription to occur. (asu.edu)
Sequence alignment showed that both CXXC domains have a very similar framework but differ in the central tip region. (cipsm.de)

Eukaryotic cells have a distinct, membrane-bound nucleus that isolates the DNA from the rest of the cell. (poly.edu)
Coverage includes membrane reactors and catalytic design (catalytic membrane reactors). (lu.se)

The DNA extraction process is a fairly simple biochemical procedure that can be divided into three major steps: breaking open the cell (lysis), destroying membranes within the cell, and precipitating the DNA out of the solution. (poly.edu)
The following sections describe how each step relates to the physical and biochemical properties of DNA. (poly.edu)

However, so far, researchers do not have tools at their disposal that would allow larger single-stranded sequences to autonomously grow and then join each other end-to-end following a molecular design plan, a capability that could generate structures and devices with diverse capabilities. (nanowerk.com)

Eukaryotic cells respond to DNA damage by activating signaling pathways that promote cell cycle arrest and DNA repair. (kegg.jp)
Eukaryotic, archaebacterial, viral and some eubacterial DNA ligases are ATP-dependent. (embl.de)

Crystallographic studies showed that the overall interactions of the DNA-binding domain with the operator and homing sites are similar, but have some different hydrogen-bonding contacts. (rcsb.org)

Recent advances in DNA microar- bones, studying mammalian entrails or ray technologies have made it possible searching with divining rods. (lu.se)

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. (nature.com)

We compared the structure and function of the CXXC domains in the DNA methyltransferase Dnmt1 and the methylcytosine dioxygenase Tet1. (cipsm.de)

A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. (nature.com)
In contrast with the I-CreI homing endonuclease, PI-PfuI possibly has two asymmetric catalytic sites at the center of a putative DNA-binding cleft formed by a pair of four-stranded beta-sheets. (rcsb.org)

[4] The road outside the new building is named Francis Crick Avenue after the 1962 joint Nobel Prize winner and LMB alumnus, who co-discovered the helical structure of DNA in 1953. (wikipedia.org)
Watson and Crick discovered the double-helical structure of DNA , which revealed that biological information was encoded in a linear structure and how this information could be duplicated during cell division . (wikipedia.org)

Our characterization of pol β from a model fish organism contributes to the study of the function and evolution of DNA polymerases, which are emerging as important cellular targets for chemical intervention in the development of anticancer agents. (biomedcentral.com)
However, the drugs also interact with other enzymes, e.g. topo I, DNA- and RNA polymerases, and helicases. (allindianpatents.com)

When it combines with CRISPR and gRNA, it specifically cleaves DNA at a programmed location (at target sequence). (geneticeducation.co.in)

We therefore studied the association of two polymorphisms - R353Q polymorphism at codon 353 involving the catalytic region and the 10 base pair (bp) insertion polymorphism involving the promoter region - with FVIIc levels in 176 healthy Tunisians. (who.int)

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Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture2

Information Science12

Health Care4

Cleavage13

Subunit6

Protein24

Polymerase4

Nucleotide5

Enzyme9

Genome12

Methylation3

Molecules7

CDNA2

Mass Spectrometry1

Reverse transcriptase2

Replication6

Exome1

Cleave2

DNAs1

Amino Acid6

Genes2

Chromatin5

Highly conserved1

Stabilization1

Binds3

Complementary to the guide2

Strands1

Vitro2

Strand4

Exon1

Hairpin3

RNAs2

Proteins3

Alignments1

Transcript1

Complexes1

Activity7

Telomerase1

Molecule2

Topoisomerase3

Synthesis1

Assay2

Genomic loci1

Inhibitor1

Inhibition1

Residue2

Bacterial species1

Genetic3

Alignment2

Membrane2

Biochemical2

Single-stranded1

Eukaryotic2

Interactions1

Mammalian1

Plasmid1

Methyltransferase1

Endonuclease2

Helical2

Polymerases2

Cleaves1

Polymorphism1