• The process involved consists of either amplification or cloning of ribosomal DNA from a bacterial population, sequencing of this ribosomal DNA, and phylogenetic analysis of the sequences obtained. (duke.edu)
  • Chromosomal regions enriched in repetitive DNA sequences have a predisposition to instability, resulting in a propensity to undergo structural rearrangements. (unesp.br)
  • Tuinen, MV , Sibley, CG & Hedges, SB 2000, ' The early history of modern birds inferred from DNA sequences of nuclear and mitochondrial ribosomal genes ', Molecular Biology and Evolution , vol. 17, no. 3, pp. 451-457. (rug.nl)
  • The aim of this study was to investigate the level of evolutionary divergence among five isofemale lineages of Metopolophium dirhodum Walker (Hemiptera: Aphididae) using sequences of the Internal Transcribed Spacer (ITS) of ribosomal DNA (rDNA). (scirp.org)
  • While ribosomal genes are highly conserved within species, ITS sequences can be intra-specifically variable. (scirp.org)
  • DNA sequences [ 4-6 ]. (who.int)
  • DNA sequences were identified to genus level and submitted to oral microbiota diversity analyses. (lu.se)
  • Although the ITS primers are universal for fungi, the D1D2 region of the large ribosomal subunit has better discrimination for yeasts, with primers NL-1 and NL-4. (cdc.gov)
  • The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
  • All inhibit protein synthesis by binding to the 50S ribosomal subunit. (msdmanuals.com)
  • This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
  • Ribosomal genes and spacers are not subjected to the same selection intensity. (scirp.org)
  • Methods: DNAs from 92 oral washings from 52 participants were subject to metabarcoding of ribosomal genes. (lu.se)
  • A subsequent analysis of mitochondrial, nuclear and ribosomal DNA only solidified this relationship. (innovations-report.com)
  • HOT1 includes an RNA polymerase I (PolI) transcription promoter that catalyzes 35S ribosomal rRNA gene transcription. (wikipedia.org)
  • To facilitate identification of R. mannitolilytica and R. pickettii , we developed 16S ribosomal DNA-based polymerase chain reaction assays that allow sensitive and specific identification of these species. (cdc.gov)
  • Mycobacterial DNA was not detected with quantitative polymerase chain reaction. (cdc.gov)
  • it inhibits RNA polymerase by binding to the DNA template-RNA polymerase complex. (msdmanuals.com)
  • In Bacteria, Archaea, and chloroplasts the rRNA is composed of different (smaller) units, the large (23S) ribosomal RNA, 16S ribosomal RNA and 5S rRNA. (wikipedia.org)
  • Application of the 5' fluorogenic exonuclease assay (TaqMan) for quantitative ribosomal DNA and rRNA analysis in sediments. (umass.edu)
  • Assay accuracy was significantly improved by employing a system of replicate dilutions and replicate analyses for both DNA and rRNA quantitation. (umass.edu)
  • Ribosomal DNA (rDNA) is a DNA sequence that codes for ribosomal RNA. (wikipedia.org)
  • From the first open reading frame of pHG12 the amino acid sequence for a polypeptide of 25 amino acid residues (designated HG12) was derived, which was identical in 22 residues with yeast ribosomal protein YL41. (nih.gov)
  • Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
  • Erratum: Sequence of 16S Ribosomal from Halobacterium volcanii, an Archaebacterium. (lu.se)
  • A fragment of yeast rDNA containing the 5S gene, non-transcribed spacer DNA, and part of the 35S gene has localized cis-acting mitotic recombination stimulating activity. (wikipedia.org)
  • These primers amplify a section of the intergenic spacer in the ribosomal cistron. (cdc.gov)
  • For identification of Trichosporon species, the intergenic region of the ribosomal cistron is used (Sugita, 2002). (cdc.gov)
  • These primers amplify approximately 600 basepairs of the ITS1-5.8S-ITS2 region of the ribosomal cistron. (cdc.gov)
  • These primers amplify approximately 620 basepairs of the 28S region of the ribosomal cistron. (cdc.gov)
  • Transcription of DNA fragments containing the coding region of pHG12 cloned into BluescriptM13, followed by cell-free translation, yielded a polypeptide with an apparent mol.wt. (nih.gov)
  • do not use an en dash between reverse transcription and PCR. (cdc.gov)
  • use a hyphen in the term real-time, but do not use an en dash between reverse transcription and PCR. (cdc.gov)
  • Scholars@Duke publication: Detection of culture-resistant bacterial pathogens by amplification and sequencing of ribosomal DNA. (duke.edu)
  • Two primer pairs, one for amplification of D1/D2- 26Sr DNA and another for the ITS1 region were used in PCR. (celljournal.org)
  • RESUME Une PCR (amplification en chaîne par polymérase) diagnostique avec des amorces spécifiques d'espèces et un séquençage direct ont été utilisés pour la détermination des membres du complexe Ano- pheles maculipennis dans les régions du nord-ouest et du centre de la République islamique d'Iran. (who.int)
  • The rDNA repeats have many regulatory mechanisms that keep the DNA from undergoing mutations, thus keeping the rDNA conserved. (wikipedia.org)
  • However, our FISH results revealed striking variability in the number and chromosomal position of rDNA clusters among the three species studied, as well as intraspecific variations in S. cyanea and S. ilheensis, which suggests intense evolutionary dynamics of this repetitive DNA family and highlights rapid chromosomal evolution in these insects. (unesp.br)
  • As a result, this DNA can provide phylogenetic information of species belonging to wide systematic levels. (wikipedia.org)
  • First phylogenetic analysis of Dryophthorinae (Coleoptera, Curculionidae) based on structural alignment of ribosomal DNA reveals Cenozoic diversification. (usda.gov)
  • Ribosomal RNA analyses demonstrated presence of additional bacterial and fungal species not identified with culture. (cdc.gov)
  • Trichophyton species DNA is amplified very poorly by the ITS primer set used for most other molds. (cdc.gov)
  • They could occur by slippage of the newly synthesized strand during DNA replication or by gene conversion. (wikipedia.org)
  • In addition, whole genome sequencing (WGS)-based digital DNA-DNA hybridization (dDDH) was used as a reference method for strain identification, and surprised with the detection of a novel W. chitiniclastica subspecies. (frontiersin.org)
  • It is therefore assumed that HG12 is the first mammalian homolog of yeast ribosomal protein YL41. (nih.gov)
  • was also observed for yeast ribosomal protein YL41. (nih.gov)
  • Instead, a molecule called a noncoding RNA, a chemical cousin of DNA, is produced from the RMRP gene. (medlineplus.gov)
  • A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
  • it does not describe the procedure for purification of fungal DNA. (cdc.gov)
  • For example, it likely helps copy (replicate) the DNA found in the energy-producing centers of cells ( mitochondria ). (medlineplus.gov)
  • DNA regions that are repetitive often undergo recombination events. (wikipedia.org)
  • During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). (cdc.gov)
  • All of the samples underwent sequencing of the 16S ribosomal DNA. (medscape.com)
  • Enhancing DNA barcode reference libraries by harvesting terrestrial arthropods at the National Museum of Natural History. (usda.gov)