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  • case
  • In one case, these enzymes cleave both DNA strands in the middle of a recognition sequence, generating blunt ends. (encyclopedia.com)
  • In addition to these steps the TRFLP protocol often includes a cleanup of the PCR products prior to the restriction and in case a capillary electrophoresis is used a desalting stage is also performed prior to running the sample. (wikipedia.org)
  • These peaks are often small in their intensity and usually form a problem in case the total intensity of the profile is low (i.e. low concentration of DNA). (wikipedia.org)
  • single
  • To overcome this problem and to increase the resolving power of this technique a single sample can be digested in parallel by several enzymes (often three) resulting in three T-RFLP profiles per sample each resolving some variants while missing others. (wikipedia.org)
  • digestion
  • B ) Digestion-elongation model for efficient amplification of DNA. (jbsdonline.com)
  • Our findings suggest that the digestion of nucleic acids (either DNA or RNA) may play an important role in the evolution of genetic material for procreating the diversification of genetic information on the early earth. (jbsdonline.com)
  • FINDINGS: The standard ChIP protocol was modified by replacing the conventional DNA fragmentation, i. e. via sonication or undirected enzymatic digestion (by MNase), through a sequence specific enzymatic digestion step. (biomedsearch.com)
  • Immunoprecipitated chromatin was analyzed by PCR using two primer sets - one for the specific detection of precipitated TFBSs and one for the validation of completeness of the enzyme digestion step. (biomedsearch.com)
  • The established site-specific enzyme digestion enables a reliable and individual detection option for densely arranged binding motifs in vivo not provided by e.g. (biomedsearch.com)
  • reaction buffer
  • Increasing the ionic strength of the solution environment, including the addition of DNA polymerase I reaction buffer significantly decreased the equilibrium dissociation constant values. (fiu.edu)
  • genomic
  • Using DNA from their own cheek cells, students learn a simple method that enables genomic biologists to analyze human DNA in this lab developed in partnership with Brookhaven National Laboratories. (wardsci.com)
  • Our method requires no prior genomic knowledge and achieves per-site and per-individual costs below that of current SNP chip technology, while requiring similar hands-on time investment, comparable amounts of input DNA, and downstream analysis times on the order of hours. (nih.gov)
  • buffer
  • Instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 ml (or 0.5 ml) microcentrifuge tubes), UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain® Blue Cards, & Disposable Pipets. (edvotek.com)
  • protein
  • The structure of M.EcoKI Type I DNA methyltransferase with a DNA mimic antirestriction protein. (mendeley.com)
  • DNA vs. protein, or arise from differentially labeled components, e.g. having one protein in a complex deuterated while the rest are protonated. (wikipedia.org)
  • The validity of this opinion was later corroborated by studies of chromosomal structure, genetic distance determined by protein variation at polymorphic loci, and mitochondrial DNA restriction enzyme analysis. (wikipedia.org)
  • Also, the Argonaute-miRNA complex can adjust protein production by recruiting cellular factors such as peptides or post translational modifying enzymes, which degrade the growing of polypeptides. (wikipedia.org)
  • polymerase
  • After extracting the DNA, a Polymerase Chain Reaction (PCR) is performed to amplify a section of the mitochondrial genome that can vary between individuals. (wardsci.com)
  • Initial DNA polymerase I binding studies (including a gel mobility shift assay and a protection assay) indicated a notable interaction between DNA polymerase I and the Bss HII site. (fiu.edu)
  • An in-depth study revealed that equilibrium binding of DNA polymerase I to the T7 RNA polymerase promoter was comparable to that of the (GC) 3 site, however the strongest interaction was observed with a cruciform containing region. (fiu.edu)
  • The high binding potential of DNA polymerase I for each of the motifs described, is hypothesized to be due to recognition of the structural DNA anomalies by the 3 ′ -5 ′ exonuclease domain. (fiu.edu)
  • genetic
  • It can be expected that when several restriction enzymes are mixed, the DNA involving more genetic information might be obtained. (jbsdonline.com)
  • If you're curious about DNA, join Felicia Vulcu and Caitlin Mullarkey, two biochemists from McMaster University, as they explore the structure of DNA, how scientists cracked the genetic code, and what our DNA can tell us about ourselves. (coursera.org)
  • Along the way, you'll learn about the practical techniques that scientists use to analyze our genetic risks, to manipulate DNA, and to develop new treatments for a range of different diseases. (coursera.org)
  • Nobel
  • He shared the 1978 Nobel Prize in Physiology or Medicine for the discovery of restriction enzymes. (wikipedia.org)
  • He was awarded the 1993 Nobel Prize in Physiology or Medicine with Phillip Allen Sharp for the discovery of introns in eukaryotic DNA and the mechanism of gene-splicing. (wikipedia.org)
  • before moving to Cold Spring Harbor Laboratory, where he was hired by James Dewey Watson, a co-discoverer of the structure of DNA and a fellow Nobel laureate. (wikipedia.org)
  • genome
  • The Bss HII restriction site (GC) 3 resides in a 9bp region of alternating pyrimidine and purine residues within the &phis;X174 genome. (fiu.edu)
  • Patterns
  • Differences between terminal region patterns of DNA restriction enzyme digests of different RCN isolates suggest that varied passaging has promoted the advancement of polymorphisms since the virus was first isolated in 1961. (wikipedia.org)
  • found
  • In many eukaryotes, including animals, the RNA interference pathway is found, and it is initiated by the enzyme Dicer. (wikipedia.org)
  • sites
  • When the sites have been methylated by MspI methyltransferase, the enzyme will cut 300 times slower than unmethylated DNA and 50 times slower if the DNA is hemi-methylated. (wikipedia.org)