• Using DNA from their own cheek cells, students learn a simple method that enables genomic biologists to analyze human DNA in this lab developed in partnership with Brookhaven National Laboratories. (wardsci.com)
  • Genomic DNA of Blastocystis isolates released into 0.1 Triton X-100 was suitable for amplification and yielded similar results as the genomic DNA extracted with standard kit. (um.edu.my)
  • Genomic DNA from the animal liver was isolated according to with some modifications described below. (sibenzyme.com)
  • Our method requires no prior genomic knowledge and achieves per-site and per-individual costs below that of current SNP chip technology, while requiring similar hands-on time investment, comparable amounts of input DNA, and downstream analysis times on the order of hours. (nih.gov)
  • Discrimination by restriction of genomic DNA with Sma I followed by PFGE enabled the identification of 14 DNA subtypes. (unifesp.br)
  • Based an the combined REAP-genomic DNA subtype, the predominant subtype in the university hospital was AIA (44 isolates) whereas the epidemic subtype in the private hospital was AIM (seven isolates). (unifesp.br)
  • A genomic library is created by isolating DNA from cells and then amplifying it using DNA cloning technology. (britannica.com)
  • The barrier to foreign DNA presented by the bbe02 and bbq67 loci of B. burgdorferi implies that genomic DNA should be modified in spirochetes carrying these plasmid genes. (asm.org)
  • After extracting the DNA, a Polymerase Chain Reaction (PCR) is performed to amplify a section of the mitochondrial genome that can vary between individuals. (wardsci.com)
  • Initial DNA polymerase I binding studies (including a gel mobility shift assay and a protection assay) indicated a notable interaction between DNA polymerase I and the Bss HII site. (fiu.edu)
  • An in-depth study revealed that equilibrium binding of DNA polymerase I to the T7 RNA polymerase promoter was comparable to that of the (GC) 3 site, however the strongest interaction was observed with a cruciform containing region. (fiu.edu)
  • Increasing the ionic strength of the solution environment, including the addition of DNA polymerase I reaction buffer significantly decreased the equilibrium dissociation constant values. (fiu.edu)
  • The high binding potential of DNA polymerase I for each of the motifs described, is hypothesized to be due to recognition of the structural DNA anomalies by the 3 ′ -5 ′ exonuclease domain. (fiu.edu)
  • DNA Polymerase of the T4-Related Bacteriophages. (elsevier.com)
  • Janakidevi, K., "Effect of Heparin or Removal of Lysine-Rich Histone Fraction on the Poly(ADP-Ribose) Polymerase, DNA Polymerase and Template Activity of Isolated Swine Aortic Nuclei", Exp. (freepatentsonline.com)
  • PCR (polymerase chain reaction) analysis is usually the first step in the creation of a DNA profile today. (howstuffworks.com)
  • First, a heat-stable DNA polymerase -- a special enzyme that binds to the DNA and allows it to replicate -- is added. (howstuffworks.com)
  • Structural requirements for binding of bovine tRNATrp with avian myeloblastosis virus DNA polymerase. (uni-trier.de)
  • Butylanilinouracil: a selective inhibitor of HeLa cell DNA synthesis and HeLa cell DNA polymerase alpha. (uni-trier.de)
  • This has made it possible for the molecular biologist to produce recombinant molecules , designer DNA and is vital for the "cloning" of genes. (coursehero.com)
  • These may then be used (1) to determine the order of genes on chromosomes, (2) to analyse the chemical structure of genes and of regions of DNA which regulate the function of genes, and (3) to create new combinations of genes. (nobelprize.org)
  • Even though these enzymes have been available only during a few years their application to genetics has already led to new and far reaching results, in particular concerning the organisation and expression of genes (= DNA) of higher animals. (nobelprize.org)
  • Sequencing and restriction analysis of genes like 16S rRNA and HSP60 are intensively used for molecular identification in the microbial communities. (hindawi.com)
  • The Lyme disease spirochete Borrelia burgdorferi has two plasmid-borne putative R-M genes, bbe02 and bbq67 , whose presence limits transformation by shuttle vector DNA from Escherichia coli . (asm.org)
  • Further studies linked the transformation phenotype of B. burgdorferi strain B31 with the bbe02 and bbq67 genes on lp25 and lp56, respectively, and the putative restriction-modification (R-M) enzymes that they encode ( 11 , 27 , 29 , 34 ). (asm.org)
  • Hoffman, et al, "A 10-Minute DNA Preparation from Yeast Efficiently Releases Autonomous Plasmids for Transformation of Escherichia coli", Gene, 57:267-272, 1987. (freepatentsonline.com)
  • To test this hypothesis, we compared the transformation of B. burgdorferi with shuttle vector DNA isolated from either Escherichia coli or B. burgdorferi , as outlined in Fig. 1 . (asm.org)
  • Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. (neb.com)
  • Restriction enzyme Associated DNA Sequencing of Barnacles with Hard and Gummy Glue Phenotypes on T2 silicone films (Session Plenary). (duke.edu)
  • DNA sequencing with Chain-terminating inhibitors. (springer.com)
  • This example shows how to use the Bioinformatics Toolbox™ to find potential primers that can be used for automated DNA sequencing. (mathworks.com)
  • Bestor T, Laudano A, Mattaliano R, Ingram V. Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. (springer.com)
  • Here, we describe the inference of the underlying mosaic founder structure for the full set of RILs from a dense set of semicodominant restriction-site-associated DNA (RAD) markers and use simulations to explore how variation in marker density and sequencing coverage affects inference. (genetics.org)
  • Before hydrolysis reaction, all DNA preparations were treated with ribonuclease A (0.1 mg/ml) for 10 minutes at a room temperature and dialyzed in DispoDialyzer MWCO 50,000 tubes ("Sigma", USA) TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) 100 times of the volume of single DNA preparation at 4°C for 20 hours. (sibenzyme.com)
  • One Kunitz unit is defined as the amount of enzyme added to 1 mg/ml salmon sperm DNA that causes an increase in absorbance of 0.001 per minute at the wavelength of 260 nm when acting upon highly polymerized DNA at 25 °C in a 0.1 M NaOAc (pH 5.0) buffer. (wikipedia.org)
  • Nucleic acids are the fundamental building blocks of DNA and RNA and are found in virtually every living cell. (elsevier.com)
  • In particular aspects, this invention relates to the use of chaotropic compositions, such as guanidine hydrochloride or guanidinium isothiocyanate, in combination with polyanionic compositions, such as those containing sulfated polysaccharides (i.e., heparin or heparitin sulfate), for the isolation of nucleic acids (RNA or DNA). (freepatentsonline.com)
  • This invention provides a specific DNA probe and describes its use in a primer directed amplification assay for E. suis. (google.com)
  • As a result, targeted amplification of 18S rRNA from clinical samples using universal primers frequently results in competitive priming and preferential amplification of host DNA. (cdc.gov)
  • 21kb DNA band undigested in agarose gels while with TaqI, no such undigested band was seen. (ias.ac.in)
  • Allan BW, Beechem JM, Lindstrom WM, Reich NO. Direct real time observation of base flipping by the EcoRI DNA methyltransferase. (springer.com)