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  • global replication
  • At synergistic drug concentrations, global replication stress was a distinct indicator of chemosensitization as characterized molecularly by an accumulation of S-phase cells with high levels of hyperphosphorylated RPA-loaded single-stranded DNA. (aacrjournals.org)
  • regulator
  • Igoshin said that when the researchers set out to find how sporulation decisions were timed to the cell cycle, several studies including prior work by team members, provided a significant clue: Under starvation conditions, the activity of the master regulator gene had been shown to spike once per cell cycle. (rice.edu)
  • Here, I established the nuclear position as a novel regulator of DNA replication timing. (tu-darmstadt.de)
  • synthesis
  • In particular, we demonstrated that when cells encounter challenges to DNA synthesis, the organization of DNA replication drives the response to replication stress that is mediated by the ATR/Rad3 checkpoint pathway, thus shaping the pattern of genome instability along the chromosomes. (springer.com)
  • Together, our results suggested a "foot-in-the-door" mechanism for drug synergy where cells were destroyed not by frank G 2 -M phase abrogation but rather by initiating a cumulative genotoxicity that deregulated DNA synthesis. (aacrjournals.org)
  • onset
  • The DNA replication onset of facultative heterochromatin was shifted toward early S-phase, as DNA content was further increased in early S-phase in treated samples. (tu-darmstadt.de)
  • Eykelenboom JK, Harte EC, Canavan L et al (2013) ATR activates the S-M checkpoint during unperturbed growth to ensure sufficient replication prior to mitotic onset. (springer.com)
  • An experimental method based on the perturbation of isogenic cell lines followed by selection, transformation and deep sequencing is presented to study the onset and the consequences of chromothripsis, a massive DNA rearrangement process observed in cancer genomes. (embopress.org)
  • alterations
  • Because alterations in DNA replication timing are associated with cancer, this discovery may lead to improved methods for cancer detection. (bio-medicine.org)
  • Alterations to the replication profile are associated with cell fate changes during development and in pathologies, but the importance of undergoing S phase with distinct and specific programs remains largely unexplored. (springer.com)
  • nucleus
  • G-band DNA is localized at the nuclear periphery, whereas R-band DNA is in the interior of the nucleus ( 7 ). (pnas.org)
  • When shattered DNA from micronuclei gets reincorporated into the primary nucleus, aberrant rearrangements can take place, a phenomenon referred to as chromothripsis. (biologists.org)
  • novo
  • In the current research, we utilizes the nuclear extracts from Saccharomyces cerevisiae which support the de novo DNA replication under physiological relevant conditions and devises single-molecule approaches to visualize the assembly or displacement of these replication factors on DNA in real-time during the origin firing. (royalsociety.org)
  • segregation
  • The "Nucleation and caging" self‐assembly mechanism is widespread to ensure faithful bacterial DNA segregation by ParAB S systems. (embopress.org)
  • constitutive
  • Using time-lapse microscopy, I was able to observe that constitutive heterochromatin, known to replicate during late S-phase, was replicated during mid S-phase when repositioned to the nuclear periphery. (tu-darmstadt.de)
  • origin
  • Direct measurement of origin firing efficiency by techniques such as DNA combing are time-consuming and lack the ability to measure all origins. (biomedcentral.com)
  • Recent genome-wide study of DNA replication approximated origin firing efficiency by indirectly measuring other quantities related to replication. (biomedcentral.com)
  • The nuclear matrix region seems to be important as a replication site, since LANA as well as cellular replication factors accumulate there and recruit the viral replication origin in latency (ori-P) by its binding activity to LBS. KSHV ori-P consists of LBS followed by a 32-bp GC-rich segment (32GC). (frontiersin.org)
  • Although it has been reported that LANA recruits cellular pre-replication complexes (pre-RC) such as origin recognition complexes (ORCs) to the ori-P through its interaction with ORCs, this mechanism does not account completely for the requirement of the 32GC. (frontiersin.org)
  • We propose that replication factors, such as Cdc6/MCM2-7/Cdc45/GINS, might dictate the time of origin firing in G1 phase. (royalsociety.org)
  • Arrest of Viral Proliferation by Ectopic Copies of Its Cognate Replication Origin. (nih.gov)
  • Heterochromatin
  • Integration of transgenes within heterochromatin (in cis) did, however, confer late replication and this was reversed after variegated transgene expression. (ox.ac.uk)
  • bacterial
  • This illustration of the replication cycle for circular bacterial DNA shows how Bacillus subtilis bacteria use the ratio of proteins KinA to Spo0F to time their decision to form spores. (rice.edu)
  • chromothripsis
  • An in vitro cell‐based model system is developed to enable studies of catastrophic DNA rearrangements, including of chromothripsis events. (embopress.org)
  • cell cycle
  • Duplication, which occurs during the S phase of the cell cycle, is initiated at many replication origins in the DNA molecule. (bio-medicine.org)
  • Combining cell-cycle checkpoint kinase inhibitors with the DNA-damaging chemotherapeutic agent gemcitabine offers clinical appeal, with a mechanistic rationale based chiefly on abrogation of gemcitabine-induced G 2 -M checkpoint activation. (aacrjournals.org)
  • Upon recruitment to damage sites, ATM and ATR phosphorylate a number of substrates, including checkpoint kinase CHK1 (CHEK1), which in turn mediate cell-cycle arrest to facilitate DNA repair ( 1 ). (aacrjournals.org)
  • researchers
  • In a mind-boggling world first, a team of biologists and security researchers have successfully infected a computer with a into a strand of DNA. (scoop.it)