• Treatment with a nucleos(t)ide analogue eliminates most HBV DNA replication intermediates and produces a gradual decline in levels of covalently closed circular DNA (cccDNA), the template for viral RNA synthesis. (osti.gov)
  • Virus DNA replication and new cccDNA synthesis were inhibited with Entecavir. (osti.gov)
  • Low level virus DNA synthesis may contribute to survival of cccDNA through mitosis. (osti.gov)
  • DNA replication creates opportunities for mutation, and the timing of DNA replication correlates with the density of SNPs across the human genome. (broadinstitute.org)
  • The DNA replication (or origin) licensing machinery ensures precise duplication of the genome and contributes to the regulation of proliferative capacity in metazoa. (lancs.ac.uk)
  • Error rates for conventionally purified DNA polymerase-alpha from calf thymus, chicken, and human sources have been reported to be one in 10,000 to one in 40,000 nucleotides incorporated. (neb.com)
  • Isolation of polymerase-alpha by immunoaffinity chromatography yields a multiprotein high molecular weight replication complex that contains an associated DNA primase (Wong, S. W., Paborsky, L. R., Fisher, P. A., Wang, T. S-F., and Korn, D. (1986) J. Biol. (neb.com)
  • Upon prolonged storage at -70 degrees C, the error rate of the immunoaffinity-purified calf thymus DNA polymerase-primase complex increases to about one in 50,000 nucleotides incorporated, an error rate similar to that exhibited by conventional isolates of DNA polymerase-alpha. (neb.com)
  • We have isolated DNA polymerase-primase complexes from calf thymus, from a human lymphoblast cell line (TK-6), and from Chinese hamster lung cells (V-79) using two different methods of immunoaffinity chromatography. (neb.com)
  • In the current study, this relationship was investigated in telomerase positive human cells using a DNA methylation sensitive variant of the Single Telomere Length Analysis (STELA) technique. (le.ac.uk)
  • No relationship between telomere length and DNA methylation at XpYp was found in telomerase positive cells. (le.ac.uk)
  • However, evidence suggests that elongated telomeres can prevent loss of subtelomeric DNA methylation associated with replication in normal human cells. (le.ac.uk)
  • Using an in vitro fibroblast model system coupled to a cell-free DNA replication assay, we have studied regulation of the origin licensing pathway during exit from and re-entry into the mitotic cell cycle. (lancs.ac.uk)
  • Ectopic geminin can block re-acquisition of DNA replication competence during re-entry into the cell cycle, indicating that geminin levels must be tightly down-regulated for escape from G0. (lancs.ac.uk)
  • We show that in the quiescent state (G0) loss of proliferative capacity is achieved in part through down-regulation of the replication licensing factors Cdc6 and Mcm2-7. (lancs.ac.uk)
  • DNA replication timing associated with point mutations far more strongly than predicted from earlier analyses and showed a stronger relationship to transversion than transition mutations. (broadinstitute.org)
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