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  • Xenopus
  • Using a Xenopus laevis egg extract replication system, we previously demonstrated that replication timing is established during early G1 phase of the cell cycle (timing decision point [TDP]), which is coincident with the repositioning and anchorage of chromatin in the newly formed nucleus. (rupress.org)
  • In this study, we have distinguished between these two possibilities, demonstrating that G2 phase chromatin lacks the determinants of a normal replication timing program upon rereplication in Xenopus egg extracts despite retaining the normal chromatin spatial organization established at the TDP. (rupress.org)
  • fork
  • Furthermore, we demonstrated a dynamic association of Hat1p with chromatin during S-phase that suggests a role of this enzyme at the replication fork. (biomedcentral.com)
  • The two most logical times for the loss of such determinants are at the replication fork, where chromatin is reassembled, or during mitosis when nuclear architecture is dismantled. (rupress.org)
  • data
  • These data support a model in which events at the TDP, facilitated by chromatin spatial organization, establish determinants of replication timing that persist independent of spatial organization until the process of chromatin replication during S phase erases those determinants. (rupress.org)
  • phase
  • In this study, we use this same system to show that G2 phase chromatin lacks determinants of replication timing but maintains the overall spatial organization of chromatin domains, and we confirm this finding by genome-wide analysis of rereplication in vivo. (rupress.org)
  • RTDs must be maintained at least until the time of replication during S phase. (rupress.org)
  • fission yeast
  • In Mitchison's lab he made substantial contributions to the study of the cell cycle in fission yeast isolating and characterising cell cycle mutants and the first identification of a gene product (DNA ligase) in these mutants. (wikipedia.org)
  • nucleotide
  • In E. coli, the direction for orisome assembly are built into a short stretch of nucleotide sequence called as origin of replication (oriC) which contains multiple binding sites for the initiator protein DnaA (a highly homologous protein amongst bacterial kingdom). (wikipedia.org)
  • sequences
  • DNA sequences also form complex 3-dimensional structures, such as telomeres, centromeres and ribosomal DNA. (reference.com)
  • In the last two decades of the 20th century, botanists exploited the techniques of molecular genetic analysis, including genomics and proteomics and DNA sequences to classify plants more accurately. (wikipedia.org)
  • pathway
  • Generally, DNA replication follows a multistep enzymatic pathway. (genome.jp)
  • This renders cancer cells particularly dependent on a proficient replication stress response for their survival, making the ATR-CHK1 pathway an attractive target for cancer treatment. (springer.com)
  • genes
  • Both the ORB sequence and the adjacent initiator gene are considerably diverse among different replication origins, while in silico and genetic analyses have indicated the specificity between the initiator genes and their cognate origins. (frontiersin.org)
  • DNA is wrapped around histones, and, by transferring an acetyl group to the histones, genes can be turned on and off. (wikipedia.org)
  • transcription
  • The molecule's structure is key to its function and replication, and it controls its own replication and transcription. (brighthub.com)
  • When it was first discovered, it was thought that acetylation of lysine neutralizes the positive charge normally present, thus reducing affinity between histone and (negatively charged) DNA, which renders DNA more accessible to transcription factors. (wikipedia.org)
  • The protein encoded by this gene is a zinc finger DNA binding transcription factor that interacts with CIP1 (p21 / CDKN1A), part of a complex with cyclin E. The encoded protein may regulate the cellular localization of CIP1. (wikipedia.org)
  • replicates
  • DNA makes proteins, but it also replicates itself by splitting the double helix down the middle to create two templates which then form the basis of a new double helix, and the process continues. (brighthub.com)
  • dependent
  • In addition, a cell-free replication system dependent on exogenous adenovirus DNA templates has been developed. (springer.com)
  • Examples of orthologs in other species include: S. pombe - cdt1 (cdc10-dependent transcript 1) Drosophila melanogaster - 'double parked' or Dup Xenopus laevis - Cdt1 DNA replication factor CDT1 has been shown to interact with SKP2. (wikipedia.org)
  • Molecular Biology
  • This book collects the Proceedings of a workshop sponsored by the European Molecular Biology Organization (EMBO) entitled "Pro- teins Involved in DNA Replication" which was held September 19 to 23,1983 at Vitznau, near Lucerne, in Switzerland. (springer.com)
  • Cdt1
  • DNA replication factor Cdt1 is a protein that in humans is encoded by the CDT1 gene. (wikipedia.org)
  • Its activity during the cell cycle is tightly regulated by its association with the protein geminin, which both inhibits Cdt1 activity during S phase in order to prevent re-replication of DNA and prevents it from ubiquitination and subsequent proteolysis. (wikipedia.org)