• NEW YORK (GenomeWeb News) - Cancer Genetics today said that it and Nikon's Italian subsidiaries have entered into a distribution agreement covering Cancer Genetics' DNA probes intended for fluorescent in situ hybridization. (genomeweb.com)
  • Finally, irradiation effects and AlGaAs resonators have been studied for the applications of DNA brush layer on GaAs as biosensor during the change of attachment probe DNA and hybridization to target DNA. (umd.edu)
  • An AlGaAs resonator used as proof of concept a change in mass by a change in resonance frequency under hybridization reaction with complementary target DNA. (umd.edu)
  • Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
  • The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)
  • Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
  • We report a reagentless and reusable electrochemical DNA sensor that exploits competitive binding and target hybridization-induced change in the signalling probe conformation for robust detection of a target DNA sequence. (rsc.org)
  • conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. (musc.edu)
  • Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. (musc.edu)
  • A scheme of probe DNA hybridization. (geneticeducation.co.in)
  • Illustration of three different conditions for DNA probe hybridization. (geneticeducation.co.in)
  • Based on the application, various types of DNA probes are Hybridization probes, PCR probes, Microarray probes (cDNA probes and oligo probes), Random probes and aptamers. (geneticeducation.co.in)
  • Hybridization probes are single-stranded oligo-sequences used in hybridization-based detection techniques like- FISH, in situ hybridization and microarray. (geneticeducation.co.in)
  • FISH (Fluorescence in situ hybridization) probes are single-stranded and labeled DNA probes that directly bind on chromosomes. (geneticeducation.co.in)
  • The presence of these two bacteria was characterized by the decrease in the absorbance of nanoparticles in case of an hybridization between the probes and their targets. (studylibid.com)
  • After checking the level of hybridization, DNA samples were used in order to find out the level of specificity of the probes. (studylibid.com)
  • In situ hybridization with a blotin-labeled DNA probe was used to detect wheatrye translocations. (illinois.edu)
  • If the identification of the captured genomic target is performed using array-based hybridization approaches, the internal region may optionally contain a probe-specific tag sequence that uniquely identifies the given probe as well as a tag-release site, which, similar to the probe-release site, is also a restriction site. (wikipedia.org)
  • Clone Y5 was sublocalized to band Yq 11.22 by hybridization to a panel of cellular DNA from patients with Y chromosome rearrangements. (barrowneuro.org)
  • Dot blot hybridization showed that the three probes displayed signals for hybridization to both S. sanguinis strain ATCC 10556 and the S. sanguinis clinical isolates. (nyu.edu)
  • DNA was extracted using the alkaline-lysis and the production of extended-spectrum - method of Birnboim and Doly [16] and was lactamases by multidrug resistant E.coli has used in dot blot hybridization. (who.int)
  • Pinpoint FISH is a fully synthetic FISH technology based on single stranded fluorescently labeled DNA oligonucleotides. (biocat.com)
  • In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)
  • Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
  • DNA probes are single-stranded and labeled oligonucleotide sequences used in FISH, PCR and DNA sequencing. (geneticeducation.co.in)
  • DNA probes are short, single-stranded and labeled complementary oligonucleotide sequences used to hybridize with the target gene or sequence. (geneticeducation.co.in)
  • Similar to MIP, padlock probes are single stranded DNA molecules with two 20-nucleotide long segments complementary to the target connected by a 40-nucleotide long linker sequence. (wikipedia.org)
  • A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
  • KromaTiD sub-CEPs make use of pericentric non-repetitive genomic DNA to provide a uniform signal from chromosome to chromosome. (biocat.com)
  • Previously, a probe-selection method based on hybridisation of genomic DNA (gDNA) was developed, which enables GeneChips to be used for species that they were not designed for. (cambridge.org)
  • After ultracentrifugation, 13C-labelled DNA, containing genomic DNA of these Methylocystis spp. (lancs.ac.uk)
  • This combination of DNA stable-isotope probing, MDA and metagenomics provided access to genomic information of a relatively large DNA fragment of these thus far uncultivated, predominant and active methanotrophs in peatland soil. (lancs.ac.uk)
  • Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. (wikipedia.org)
  • these probes hybridize to and capture the genomic target. (wikipedia.org)
  • MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. (wikipedia.org)
  • Anneal probe to genomic target DNA Probes are added to the genomic DNA sample. (wikipedia.org)
  • In this linearized probe the universal PCR primer sequences are located at the 5' and 3' ends and the captured genomic target becomes part of the internal segment of the probe. (wikipedia.org)
  • If array-based approach is used, the probe may optionally contain a probe-specific tag that uniquely identifies the probe as well as the genomic region targeted by it. (wikipedia.org)
  • Multiplex analysis Although each probe examines one specific genomic locus, multiple probes can be combined into a single tube for multiplexed assay that simultaneously examines multiple loci. (wikipedia.org)
  • EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)
  • Molecular beacon probes are a sequence of nucleotides (the building blocks of DNA and RNA) that can be used to fluorescently detect the presence of a specific sequence of DNA or RNA. (edinst.com)
  • In developing a probe, a sequence of nucleotides must be identified, isolated, reproduced in sufficient quantity, and tagged with a label that can be detected. (enzolifesciences.com)
  • Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
  • The researchers looked at areas of the genome with tandem repeats - stretches of 2 to 20 nucleotides, which are the 'building blocks' of DNA, that are repeated two or more times in one spot. (spectrumnews.org)
  • for example, general FISH probes are a few hundred nucleotides long. (geneticeducation.co.in)
  • While special types of probes for larger chromosomal alteration are a few thousand nucleotides long. (geneticeducation.co.in)
  • Gap filling The gap is filled by DNA polymerase using free nucleotides and the ends of the probe are ligated by ligase, resulting in a fully circularized probe. (wikipedia.org)
  • Centromere Enumeration Probes (sub-CEPs) from KromaTiD are available for all human chromosomes as standard products. (biocat.com)
  • Telomeric Probes (sub-Telos) from KromaTiD are available for the p- and q-arms of all human chromosomes as standard products, other sequenced species are available by request. (biocat.com)
  • The probe containing a 120-bp repetitive DNA sequence from rye, hybridized to the entire length of all rye chromosomes, but only to a few sites in 14 wheat chromosomes. (illinois.edu)
  • The overall distribution of this DNA probe in the rye chromosomes has not been detected previously with the use of radioactively labeled probes. (illinois.edu)
  • Clone Y2, also found in female and male DNA, is probably located in the pseudosutosomal region because extra copies of either the X or Y chromosomes increased Y2 restriction enzyme fragment intensity in total cellular DNA. (barrowneuro.org)
  • Three chromosome-specific repetitive probes labeled with either amino acetyl fluorene (AAF), mercury, or biotin were hybridized simultaneously to metaphase chromosomes prepared from human blood lymphocytes or to interphase tumor nuclei. (nih.gov)
  • Molecular beacon probes can be custom designed to target specific DNA or RNA sequences, which allows molecular beacons to be used for real-time detection and quantification of DNA and RNA. (edinst.com)
  • 60°C was found to be an adequate temperature for the hairpins to be completely open, with the emission at 60°C being 50 times higher than at 20°C. Based on these results 60°C was chosen as the incubation temperature for DNA detection. (edinst.com)
  • In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)
  • Redmond Red, a fluoropore containing a redox-active phenoxazine core, has been explored as a new electrochemical probe for the detection of abasic sites in double-stranded DNA. (caltech.edu)
  • FISH probes are used for the detection of larger deletion, duplication and translocation which can not be assessed by conventional karyotyping. (geneticeducation.co.in)
  • High-bandwidth detection of short DNA in nanopipettes. (imperial.ac.uk)
  • Recently, we have demonstrated high-bandwidth detection of double-stranded (ds) DNA with microsecond time resolution in nanopipettes, using custom-designed electronics. (imperial.ac.uk)
  • During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). (cdc.gov)
  • In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)
  • was separated from 12C DNA and subjected to multiple displacement amplification (MDA) to generate sufficient DNA for the preparation of a fosmid metagenomic library. (lancs.ac.uk)
  • Captured target enrichment If the probe is linearized, traditional PCR amplification is performed to enrich the captured target using the universal primers of the probe. (wikipedia.org)
  • Otherwise, rolling circle amplification is performed for the circular probe. (wikipedia.org)
  • The GEN-PROBE APTIMA trichomonas vaginalis assay combines the technologies of target capture, Transcription-Mediated Amplification (TMA), and Dual Kinetic Assay (DKA). (cdc.gov)
  • The RAPD (Random Amplification Polymorphic DNA) allows the amplification of DNA sequences and is a simple and quick technique that does not require prior knowledge on the genomes to characterize organisms, using one randomly determined (usually a decamer) primer 17 . (bvsalud.org)
  • The objective of the study was to develop a rapid method to detect Mycobacterium tuberculosis from clinical samples and the applicability of DNA amplification techniques to a developing country like Sri Lanka. (who.int)
  • Phenantridine alkaloids such as sanguinarine, chelerytrine, chelirubine, chelilutine, sanguilutine and macarpine interact with double stranded DNA showing substantial changes in fluorescence emission. (muni.cz)
  • Subtle structural alterations in G-quadruplex DNA regulate site specificity of fluorescence light-up probes. (bvsalud.org)
  • In here, we have developed a compound with ability to specifically signal a certain c-MYC G4 DNA structure through a fluorescence light -up mechanism. (bvsalud.org)
  • Despite the compound's two binding sites on the G4 DNA structure, only one of them result in the fluorescence light -up effect. (bvsalud.org)
  • The objective of the present study was to design a PCR-generated DNA probe and determine the specificity of the probe for the identification of clinical isolates of Streptococcus sanguinis. (nyu.edu)
  • Rapid identification of up to three Candida species in a single reaction tube by a 5' exonuclease assay using fluorescent DNA probes. (musc.edu)
  • We examined DNA methylation levels at more than 472,506 CpG probes through the Illumina Infinium HumanMethylation450 BeadChip assay. (cdc.gov)
  • A nucleotide sequence with complementary bases to the molecular beacon loop is called complementary DNA (cDNA). (edinst.com)
  • If the complementary region is not present at the target location, the probe will not bind and not give signals. (geneticeducation.co.in)
  • Polynuclear ruthenium complexes were reported to exhibit synergistic and/or complementary effects: the enhanced DNA structural recognition and DNA binding as well as in vitro anticancer activities. (eurekaselect.com)
  • Specifically, the two target complementary regions at the 5' and 3' ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. (wikipedia.org)
  • After a denaturation followed by an annealing step, the target-complementary ends of the probe are hybridized to the target DNA. (wikipedia.org)
  • The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
  • Messenger RNA (mRNA) from the sample of interest can serve as a template for producing complementary DNA (cDNA) in the presence of a reverse transcriptase enzyme. (medscape.com)
  • The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
  • Common genetic testing techniques that we use routinely in our lab are FISH, karyotyping, PCR, DNA microarray , DNA sequencing and restriction digestion. (geneticeducation.co.in)
  • Among these techniques, FISH, PCR, DNA sequencing and microarray have one common special requirement- DNA probes . (geneticeducation.co.in)
  • Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. (medscape.com)
  • using DNA microarray technology. (lu.se)
  • The Pinpoint FISH TP53/CEP 17 Probe Kit is intended to detect the copy number of the LSI TP53 probe target located at chromosome 17q11.1 and of the CEP 17 probe target located at the centromere of chromosome 17. (biocat.com)
  • The Vysis CEP 12 SpectrumOrange DNA Probe Kit is intended to detect AT rich alpha satellite sequences in the centromere region of chromosome 12 in conjunction with routine diagnostic cytogenetic testing. (molecularcatalog.abbott)
  • The CEP 12 DNA Probe Kit has been optimized only for identifying chromosome 12 in interphase nuclei from peripheral blood specimens from patients with B-cell chronic lymphocytic leukemia. (molecularcatalog.abbott)
  • Isolation and characterization of Y chromosome DNA probes. (barrowneuro.org)
  • and Golbus, M S, "Isolation and characterization of Y chromosome DNA probes. (barrowneuro.org)
  • Besides premade probes, also genome wide, custom Pinpoint FISH assays engineered to meet your specific requirements are offered. (biocat.com)
  • Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)
  • DNA probes are artificially synthesized oligonucleotide sequences. (geneticeducation.co.in)
  • DNA microarrays are simply platforms that consist of small solid supports onto which the sequences from thousands of different genes are attached at fixed locations. (medscape.com)
  • Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
  • DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
  • While culture tests can take days, molecular probe assays can be performed within a few hours or minutes. (enzolifesciences.com)
  • thereafter, DNA probes (AccuProbe and GenoType line-probe assays [Hain Lifescience GmbH, Nehren, Germany]) were used. (cdc.gov)
  • The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
  • It involves randomly nicking the backbone of a double-stranded DNA with dilute concentrations of DNase I. At extremely low concentrations, this enzyme nicks a template at four or five sites, producing a free 3′-OH group that can act as a primer at each nicking location. (enzolifesciences.com)
  • The response of luminescence intensity to low concentrations of double stranded DNA in presence of alkaloids was found to be linear and can be potentially used for quantification of DNA by means of luminescence spectrometry. (muni.cz)
  • In this study, we demonstrate that gDNA-based probe selection on the Affymetrix Human U133+2 GeneChip array can be used to study gene expression profiles in sheep tissues. (cambridge.org)
  • The discovery of DNA markers closely linked to the gene for Huntington's disease (HD) has allowed development of predictive and prenatal testing programmes for HD. (bmj.com)
  • A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
  • DNA probes carry the information which we use to study a target sequence or gene. (geneticeducation.co.in)
  • Three digoxigenin-labeled DNA probes were synthesized on the basis of the sequence of the 1.6-kb fragment: the sequence of probe SSA-1 contained the proton-translocating ATPase (uncC) and the entire intergenic region, the sequence of probe SSA-2 contained only the intergenic region, and the sequence of probe SSA-3 contained an internal region of the murA gene. (nyu.edu)
  • Two DNA probes isolated from the region 5′ to the HLA-B gene are described. (umn.edu)
  • Because the locations of the probes are known, the intensity and pattern of the labeled mRNA can be used to measure the expression of the targeted gene. (medscape.com)
  • Even though many different types of surfaces have been studied as substrates for deoxyribonucleic acid (DNA) attachment, the development of a new type of substrate, which is reproducible, stable, highly controlled and easily transferred to practical applications, is still needed. (umd.edu)
  • The electrochemical behavior of Redmond Red-modified DNA at gold surfaces exhibits stable, quasi-reversible voltammetry with a midpoint potential centered around −50 mV versus NHE. (caltech.edu)
  • were further confirmed by DNA stable-isotope probing analysis of a sample taken from the Moor House peatland (England). (lancs.ac.uk)
  • Active microorganisms have been identified previously using stable isotope probing (SIP) or bromodeoxyuridine (BrdU) labeling. (nature.com)
  • The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)
  • Exonuclease treatment removes these non-reacted probes as well as any remaining linear DNA in the reaction. (wikipedia.org)
  • Nick translation is efficient for both linear and covalently closed DNA molecules, and labeling reaction are completed in less than an hour. (enzolifesciences.com)
  • Redmond Red, reporting only if well-stacked in the duplex, represents a sensitive probe to detect abasic sites electrochemically in a DNA-mediated reaction. (caltech.edu)
  • The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
  • Besides demonstrating that the probe selection method can be used to study the ovine transcriptome, the benefits of this approach are that it can greatly increase the number of annotated and novel genes that can be studied beyond those currently possible using ovine- or bovine-specific microarrays. (cambridge.org)
  • A search for a similar sequence in the GenBank database with the BLASTN program revealed that the 1.6-kb DNA fragment comprised an intergenic region between two housekeeping genes, uncC (proton-translocating ATPase) and murA (UDP-N-acetylglucosamine enolpyruvyl transferase). (nyu.edu)
  • Therefore, in our study, E. coli complex, multi-factorial mechanism involv- isolates from diarrhoeal cases were ana- ing a large number of virulence factors that lysed using specific DNA probes for genes vary with pathotype. (who.int)
  • Isolates were screened for presence different toxins and 2 (2.2%) isolates were of genes encoding LT and ST enterotoxins, positive with all 4 DNA probes. (who.int)
  • The architecture and density of immobilized probe molecules depend on the type of the solid surface on which they are anchored. (umd.edu)
  • In the case of the longer, more flexible DNA with 100 bases, the DNA molecules strongly interacted with each other and formed big cluster, of 330~440nm in diameter on the surface. (umd.edu)
  • Small molecules that target specific G4 DNA structures and signal their presence would therefore be of great value as chemical research tools with potential to further advance towards diagnostic and therapeutic developments. (bvsalud.org)
  • This review overviews some representative polynuclear ruthenium complexes acting as DNA structural probes, DNA binders and in vitro anticancer agents, which were developed during last decades. (eurekaselect.com)
  • The internal region contains two universal PCR primer sites that are common to all MIPs as well as a probe-release site, which is usually a restriction site. (wikipedia.org)
  • Results obtained from Restriction fragment length polymorphism typing show that the mahority of circulating Mycobacterium tuberculosis strains in Sri Lanka belong to a limited number of families, but the degree of IS6160 DNA polymorphism among strains were high. (who.int)
  • Its technology in combination with Cancer Genetics' DNA-FISH probes "will allow laboratories to perform simultaneous multicolor FISH testing with complex image analysis in academic and clinical settings," Cancer Genetics, which went public last month, said. (genomeweb.com)
  • Synthetic targets were also designed so that their level of complementarity with their respective probe was fully complement, or with mismatches of 4 and 6 bases. (studylibid.com)
  • Of the thousands of plaques screened, 13 did not hybridize to radiolabeled 46,XX total chromosomal DNA. (barrowneuro.org)
  • Chelirubine and macarpine were even successfully used for fluorescent DNA-specific staining of living cells due to the lower toxicity. (muni.cz)
  • The DNA probe hybridizes with a specific mRNA, if present. (musc.edu)
  • The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. (musc.edu)
  • Salmonella typhi's spesific probe was conjugated to gold nanoparticles and Escherichia coli's specific probe was conjugated to silver nanoparticles while universal probe for each bacteria was conjugated to magnetic beads. (studylibid.com)
  • Clone Y5 was male specific in three of four restriction enzyme digests although in the fourth a light hybridizing band was observed in both male and female DNA. (barrowneuro.org)
  • The probe SSA-2-specific intergenic region appeared to be specific for S. sanguinis. (nyu.edu)
  • The results from this study suggest that probe SSA-2 may serve as a species-specific DNA probe for the identification of clinical isolates of S. sanguinis. (nyu.edu)
  • However, this study shows that it is indeed possible to develop such compounds and present insights into the molecular details of specific G4 DNA recognition and signaling to advance future studies of G4 biology . (bvsalud.org)
  • Specific probes bind to the DNA, in order to determine what type of polio present. (cdc.gov)
  • In conclusion, we have demonstrated that natural products, phenantridine alkaloids, can be used as alternative probes to synthetic DNA dyes such as ethidium bromide. (muni.cz)
  • Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. (musc.edu)
  • Ideally, DNA probes can be labeled with fluorescent, radioactive, Biotin, chemiluminescent, Enzyme or any chemical labeling depending on the requirement of the experiment. (geneticeducation.co.in)
  • It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. (nih.gov)
  • Glass or quartz nanopipettes have found increasing use as tools for studying the biophysical properties of DNA and proteins, and as sensor devices. (imperial.ac.uk)
  • An example of the The antibiotic susceptibility test showed results of the dot blot test with the EAST-1 that 140 (70.0%) isolates carried resistance probe is shown in Figure 1. (who.int)
  • To do this, we examined over 200 arbitrarily primed PCR (AP-PCR) amplicon patterns obtained with DNA from clinical isolates of S. sanguinis. (nyu.edu)
  • A 1.6-kb DNA amplicon that was common to all AP-PCR profiles was extracted from agarose gels and then cloned and sequenced. (nyu.edu)
  • In this study, I aim to understand the attachment of nucleic acid onto the surfaces of As-terminated GaAs- based semiconductors and focus on improving the "brush-structure", which is essential for high quality of biochip based on a DNA layer. (umd.edu)
  • Thomas, J.A. Ruthenium(II) polypyridyl complexes and DNA--from structural probes to cellular imaging and therapeutics. (eurekaselect.com)
  • DNA Probes" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (musc.edu)
  • Probe release In some versions of the protocol, the probe-release site (commonly a restriction site) is cleaved by restriction enzymes such that the probe becomes linearized. (wikipedia.org)
  • The tags from each probe are released by cleaving the tag release site with restriction enzymes. (wikipedia.org)
  • Clone Y1 hybridized to multiple restriction enzyme fragments in both male and female DNA with more intense bands in male DNA. (barrowneuro.org)
  • The purified fragments analysis showed that 92 (46.0%) of the were used as probes after labelling with a 200 isolates examined were toxigenic. (who.int)
  • In recent decades, traditional methods of microorganism phenotyping have been replaced or added by the procedures associated to recombinant DNA 12-14 . (bvsalud.org)
  • For the DNA extraction and purification from the clinical samples two methods, the standard phenol extraction procedure desctibed by Sambrook etal (1982) and the guanidinium thiocyanate method described by Boom et al(1990) were used. (who.int)
  • Extra repeats in DNA may contribute to some of autism's heritability. (spectrumnews.org)
  • These repeats can expand when they are passed down from parents to children: If a nucleotide, or combination of them, is repeated 10 times in a parents' DNA, it may be repeated hundreds of times in their child, for example. (spectrumnews.org)
  • DNA hybridised to the rye 1RS chromatin under high stringency conditions, indicating the presence of shared tandem repeats among the cereals. (nature.com)
  • Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
  • Importantly, with Redmond Red positioned opposite an abasic site within the DNA duplex, the electrochemical response is significantly enhanced compared to Redmond Red positioned across from a base. (caltech.edu)
  • Keberadaan bakteri ditandai dengan turunnya nilai absorbansi dari nanopartikel, yang mengindikasikan telah terjadi proses hibridisasi antara probe dengan target. (studylibid.com)
  • Dalam penelitian ini, penulis merancang probe spesifik bakteri Escherichia coli dan Salmonella typhi yang digunakan untuk dapat terhibridisasi pada target DNA sampel, dan membuat target sintetik dengan berbagai variasi, yaitu fully complement, mismatch 4 basa, dan mismatch 6 basa. (studylibid.com)
  • With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. (wikipedia.org)
  • These probes, however, are designed such that a gap delimited by the hybridized ends of the probes remains over the target region. (wikipedia.org)
  • The captured target can also be identified by sequencing the probe, now also containing the target. (wikipedia.org)
  • The intertwined relation between the presented factors is likely the reason for the lack of examples using rational design to develop compounds with turn-on emission that specifically target certain G4 DNA structures. (bvsalud.org)
  • SIP encompasses a series of methods that involve the incorporation of heavy isotopes into newly synthetized DNA and its separation on a density gradient 16 . (nature.com)
  • The use of molecular beacons, coupled with a sensitive spectrofluorometer and sample temperature control, facilitates the measurement of extremely low concentrations of DNA or RNA. (edinst.com)
  • In addition, I studied the effect of DNA length and the presence of a good solvent, such as water, on the oligonucleotides on a GaAs surface. (umd.edu)
  • However, the FOIA documents suggest the presence of three tubes of DNA, conflicting with the Secret Service's initial reports. (thegatewaypundit.com)
  • iv Perancangan Probe DNA Biosensor Berbasis UV Spektrofomotri (Aplikasi pada Salmonella dan E.coli) Arief M. Firmansyah Pembimbing : (1) Kestrilia Rega Prilianti, S.Si. (studylibid.com)
  • Diketahui pula biosensor yang digunakan dapat memberikan hasil yang baik pada suhu tertentu, yaitu 55°C. Kata kunci: biosensor, probe, nanopartikel emas, nanopartikel perak v Design Probe for DNA Biosensor Base on UV Spectrofotometer (Aplication for Salmonella and E.coli) Arief M. Firmansyah Pembimbing : (1) Kestrilia Rega Prilianti, S.Si. (studylibid.com)
  • Below are the most recent publications written about "DNA Probes" by people in Profiles. (musc.edu)