Dna polymerase yields. Medical search. Frequent questions

The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification. (neb.com)
Hot Start Taq DNA Polymerase allows for greater amplification sensitivity and increased ease of reaction setup. (neb.com)
It is being increasingly used in molecular biology for multiple displacement DNA amplification procedures, and has a number of features that make it particularly suitable for this application. (wikipedia.org)
Φ29 polymerase enzyme is already used in multiple displacement amplification (MDA) procedures (including in a number of commercial kits) whereby fragments tens of kilobases in length can be produced from non-specific hexameric primers annealing at intervals along the genome. (wikipedia.org)
Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. (sigmaaldrich.com)
The Expand ™ High Fidelity PLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. (sigmaaldrich.com)
Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification). (qiagen.com)
REPLI‑g Kits provide DNA polymerase, buffers and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue and cells. (qiagen.com)
The REPLI-g Mini and Midi Kits offer a single tube workflow with higher yields of amplified DNA, while the REPLI-g UltraFast Kit enables highly uniform and accurate whole genome amplification in just 60-90 minutes. (qiagen.com)
Use Platinum Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. (thermofisher.com)
Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment. (neb.com)
in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp ™ seed PCR kit. (sigmaaldrich.com)
High Specificity & Yield - JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity. (sigmaaldrich.com)
Have greater confidence in library amplification results with Codex® HiFi Hot Start DNA Polymerase, delivering greater library complexity, high yields, and enhanced uniformity of coverage. (codexis.com)
How should I set up an amplification reaction using Taq DNA Polymerase? (genscript.com)
How is the amplification of a gene sample of interest carried out using Polymerase Chain Reaction (PCR)? (mcqexams.com)
Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. (mcqexams.com)
Long range PCR refers to the amplification of DNA targets over 5kb in length which typically cannot be amplified using routine PCR methods or reagents. (wazeesupperclub.com)
Therefore, the DNA used as a template in the PCR reaction plays an important role in amplification. (wazeesupperclub.com)
The main application of the Expand Long Template PCR System is the accurate amplification of very long target sequences, even from genomic DNA. (wazeesupperclub.com)
The MasterMix also contains Epicentre's PCR Enhancer, which substantially improves yield, efficiency, and specificity of amplification of many target sequences, especially those with a high GC content or secondary structure. (genengnews.com)
NZYTaq II DNA polymerase lacks 3'→5' exonuclease activity and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. (nzytech.com)
The enzyme was optimized to provide higher sensitivity, allowing amplification of different DNA fragments from as little as 5 pg of human genomic DNA. (nzytech.com)
What to do when there is no product amplification or low yield? (nzytech.com)
It provides robust amplification of difficult templates including plasmids, BACS, genomic DNA, and lambda DNA. (carolinabiosystems.cz)
It allows the amplification of up to 8kb human genomic DNA and up to 21kb lambda DNA. (carolinabiosystems.cz)
Taq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer that minimizes the need for optimization of PCR parameters, as well as Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g. (qiagen.com)
25 Cycles of PCR amplification of 5 ng Lambda DNA with 1.25units of Taq DNA Polymerase in the presence of 200 µM dNTPs and 0.2 µM primers results in the expected 5 kb product. (biolmedonline.com)
biotechrabbit™ YourTaq Hot Start PCR Master Mix is optimized for high yield of amplification of 0.1-3 kb DNA targets, even from low copy number. (biotechrabbit.com)
YourTaq Hot Start PCR Master Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. (biotechrabbit.com)
When assembling the amplification reactions, care should be taken to eliminate the possibility of contamination with undesired DNA. (biotechrabbit.com)
A high-fidelity, aptamer-based hot start DNA polymerase tailored for low GC bias amplification. (meridianlifescience.com)
A high-fidelity DNA polymerase with aptamer-based hot-start technology highly suited to target enrichment, NGS library amplification, and cloning applications. (meridianlifescience.com)
High-Specificity Pfu HS Mix provides high yield and sensitivity, as well as superior amplification of GC- and AT-rich targets. (meridianlifescience.com)
The hot-start aptamer binds reversibly to the polymerase which inhibits its activity at ambient temperatures and improves the specificity of the amplification. (meridianlifescience.com)
High sensitivity and yield PCR amplification of a 1.2 kb fragment of the F8 gene, from a five-fold serial dilution of human genomic DNA. (meridianlifescience.com)
After a series of successive cycles of amplification, the presence of double stranded DNA product was visualized on agarose gels stained with ethidium bromide. (cdc.gov)

Various clinical and non-clinical research samples can be used with REPLI-g Mini and Midi Kits, including genomic DNA, fresh or dried blood, fresh or frozen tissue and cells. (qiagen.com)
The cellular response to DNA damage involves an intricate network of enzymes responsible for sensing, signaling, and repairing damaged DNA, as well as the regulation of cell cycle checkpoints that collectively maintain genomic integrity 2 . (nature.com)
A 500 bp human genomic DNA target was used to compare the two leading competing QPCR Master Mixes. (bio-helix.com)
This powerful polymerase mixture produces a high yield of PCR product from genomic DNA. (wazeesupperclub.com)
For high template quality, use Roche′s High Pure Template Preparation Kit to prepare genomic DNA template. (wazeesupperclub.com)
Each reaction included 1X MasterMix, 12.5 pmol each of forward and reverse primers, and 50 ng of human genomic DNA. (genengnews.com)
Some DNA isolation procedures, particularly genomic DNA isolation, can result in the co-purification of PCR inhibitors. (nzytech.com)
Human genomic DNA was amplified using the PCR Master Mix and specific primers to produce a distinct band. (biotechrabbit.com)
This robust enzyme mix with proofreading activity will polymerize probes 40 bp to 5 kb long using 10 pg plasmid DNA and 10 ng genomic DNA as template. (thomassci.com)
A specific fragment pattern is detected after hybridization of the PCR product to 10μg human genomic DNA followed by chemiluminescent detection. (thomassci.com)
The following regions from human genomic DNA were amplified: RBB8 161 bp (29% GC), RBB8 450 bp (32% GC), IL 10 417 bp (44% GC), Mpex 418 bp (55% GC), EGFR 525 bp (62% GC), EGFR 380 bp (65% GC). (meridianlifescience.com)

The 'hot start' property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield. (thermofisher.com)
After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of 200~400 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. (nih.gov)
Will the 5'→3' flap endonuclease activity of Taq DNA Polymerase degrade primers? (genscript.com)
After adapter ligation, ChIP DNA was PCR-amplified with Illumina primers for 18 cycles and library fragments (ChIP) (insert plus adaptor and PCR primer sequences) were size selected from a 8% polyacrylamide gel. (nih.gov)
A type of RNA polymerase, it sets the starting point for replication by adding small RNA primers. (aboutdarwin.com)
Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand at 54$$^0$$C. (mcqexams.com)
Using the PCR ingredients like dNTPs, PCR buffer, DNA primers and template DNA, copies of DNA can be generated in a PCR. (wazeesupperclub.com)
The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. (gentaur.com)
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). (gentaur.com)
The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. (gentaur.com)
The reaction mix composition may affect the melting properties of primers and DNA. (nzytech.com)
This RNA-dependent DNA polymerase, with the assistance of reverse transcription primers, deoxynucleotides (dNTPs: dATP, dTTP, dGTP and dCTP) and enzyme cofactors, catalyzes the synthesis of cDNA from its respective target RNA sequence. (stratech.co.uk)
The reaction temperature is briefly cooled to 40 to 60°C and then raised to 70 to 75°C. Primers anneal to the complementary sequence during the cooling phase and extend the primers with Taq polymerase during the heating phase. (ojp.gov)
During PCR testing, Taq polymerase and DNA primers complementary to a unique sequence of target DNA were used to greatly amplify that region if the target was present in the sample. (cdc.gov)

The following guidelines are provided to ensure successful PCR using New England Biolabs' Hot Start Taq DNA Polymerase. (neb.com)
Mg++ concentration of 1.5-2.0 mM is optimal for most PCR products generated with Hot Start Taq DNA Polymerase. (neb.com)
We generally recommend using Hot Start Taq DNA Polymerase at a concentration of 25 units/ml (1.25 units/50 μl reaction). (neb.com)
However, the optimal concentration of Hot Start Taq DNA Polymerase may range from 5-50 units/ml (0.25-2.5 units/50 μl reaction) in specialized applications. (neb.com)
Hot Start Taq DNA Polymerase does not require a separate activation step. (neb.com)
Hot Start Taq DNA Polymerase （ Antibody modification ） is a hot-start thermostable DNA polymerase from Thermus aquaticus YT-1 ，that possesses a 5´→ 3´ polymerase activity and a 5 ´ flap endonuclease activity. (biolmedonline.com)
The hot-start Taq DNA polymerase is a Taq DNA polymerase which modified by thermolabile Taq antibodies. (biolmedonline.com)

Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer. (thermofisher.com)
Platinum Taq Green Hot Start DNA Polymerase is provided with green PCR buffer that contains a density reagent and two tracking dyes and allows for direct gel loading of PCR products. (thermofisher.com)
Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. (thermofisher.com)
The REDExtract-N-Amp ™ PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase. (sigmaaldrich.com)
Taq DNA polymerase can have 100% activity in the supplied GenScript Taq Buffer. (genscript.com)
GenScript High-Stability PCR Kit contains Green Taq DNA polymerase, 10× Taq buffer, and Stabilized dNTP Mix, 10 mM Each, all specially formulated for increased stability using our proprietary technology. (genscript.com)
Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. (gentaur.com)
The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. (jenabioscience.com)
Primer extensions are usually performed at 72°C. Estimates for the rate of nucleotide incorporation at 72°C vary from 35 to 100 nucleotides per second , depending upon the buffer, pH, salt concentration, and the nature of the DNA template. (ojp.gov)
Control Template, plasmid DNA in Tris/EDTA buffer, pH 8.0. (thomassci.com)
Hier finden Sie Informationen und weiterführende Literatur zu Ampliqon Taq DNA Polymerase with Ammonium buffer. (genaxxon.com)

Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. (thermofisher.com)
Name the bacterium that yields a thermostable DNA polymerase. (mcqexams.com)
Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. (wazeesupperclub.com)
Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5'-3' synthesis of DNA. (abmgood.com)

It contains NanoTaq hot-start DNA polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers and essentials for a success PCR reaction. (bio-helix.com)
The TAQXpedite PCR System contains a blend of thermostable DNA polymerases, an optimized 2X Universal MasterMix, and a Difficult/Long Master Mix with all four dNTPs. (genengnews.com)
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR. (genaxxon.com)

it comprises a C-terminal polymerase domain and a spatially separated N-terminal domain with a 3'-5' exonuclease activity. (wikipedia.org)
Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity. (thermofisher.com)
Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. (neb.com)
This polymerase has 5'-3' exonuclease activities, lacks 3'-5' exonuclease activity, and produces 3'-dA-tailed amplicons. (abmgood.com)
Ampliqon Taq DNA Polymerase exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. (genaxxon.com)

DNA by Polymerase Chain Reaction Method. (bvsalud.org)
Appropriately Selected Nerve in Suspected Leprous Neuropathy Yields High Positive Results for Mycobacterium leprae DNA by Polymerase Chain Reaction Method. (bvsalud.org)
Identification of Mycobacterium leprae DNA by polymerase chain reaction ( PCR ) is a reliable and an affordable method to confirm leprosy . (bvsalud.org)
The average DNA yield from the REPLI-g UltraFast Mini Kit is 7-10 µg per 20 µl reaction in 90 minutes with standard quality samples. (qiagen.com)
The poly(A) tail is encoded in the DNA template, which eliminates reaction steps and reduces overall production time and material loss [19] . (researchgate.net)
What type of DNA end results from a primer extension reaction or a PCR using Taq DNA Polymerase? (genscript.com)
When should Taq DNA Polymerase be used in a primer extension reaction or for PCR? (genscript.com)
It is used in the polymerase chain reaction or PCR, a method which is used to amplify the quantity of short segments of DNA. (mcqexams.com)
PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. (gentaur.com)
Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. (gentaur.com)
Each reaction contained 1X TAQXpedite Universal MasterMix, 12.5 pmol each of forward and reverse primer, and 1 ng of template DNA. (genengnews.com)
Reduce the volume of template DNA in reaction or dilute template DNA prior to adding to the reaction. (nzytech.com)
Reverse transcription polymerase chain reaction (RT-PCR) is a core molecular technique designed to detect and quantitate total RNA or messenger RNA (mRNA), with a high degree of sensitivity and accuracy. (stratech.co.uk)
Animated video tutorial illustrating the key steps in reverse transcription polymerase chain reaction: (1) reverse transcription, (2) denaturation, (3) primer annealing, (4) primer extension. (stratech.co.uk)
Since both reverse transcription and PCR take place in the same tube the reaction conditions cannot be separately optimized, which can impact yield or reaction efficiency to varying degrees. (stratech.co.uk)
Selected cells were administered early following transplantation, either after the detection of cytomegalovirus DNA by polymerase chain reaction-based surveillance or prophylactically between day 40 and day 50. (ox.ac.uk)
Incubation of a 50 µL reaction containing a minimum of 12.5 U of Taq DNA Polymerase with 10 nmol 5´- FAM oligonucleotide for 30 minutes at 37℃ yields no detectable degradation. (biolmedonline.com)
b: The optimal concentration of Taq DNA Polymerase may range from 5-50 units/ml (0.1-0.5 units/25 µL reaction) in specialized applications. (biolmedonline.com)
With every PCR setup, perform a contamination control reaction that does not include template DNA. (biotechrabbit.com)
For convenient and efficient generation of DIG-labeled DNA probes in polymerase chain reaction (PCR). (thomassci.com)
One PCR labeling reaction (50 Î¼l) will typically yield enough probe for 20 ml hybridization solution. (thomassci.com)
[ 3 ] Also available is a polymerase chain reaction (PCR) assay that detects B burgdorferi DNA. (medscape.com)
Polymerase chain reaction (PCR): PCR is a test used to locate and make copies of parts of the DNA contained in the castor bean plant. (cdc.gov)
Diagnosis requires specific growth media, serologic or urine antigen testing, or polymerase chain reaction analysis. (msdmanuals.com)
In addition, polymerase chain reaction testing with DNA probing is available and may help identify transmission pathways. (msdmanuals.com)
Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)
In terms of accuracy, the results resemble those possible with slower polymerase chain reaction tests, which can take 1 to 6 hours. (medscape.com)
A quantitative polymerase chain reaction assay (115 bp amplicon) specific to Escherichia coli K12 with an ABI(TM) internal control was developed based on sequence data encoding the rfb gene cluster. (who.int)
In the present work, we analyzed aberrations in G-banded chromosomes, reproductive hormone levels, and polymerase chain reaction-based V(D)J rearrangement frequencies in applicators whose exposures were mostly limited to chlorophenoxy herbicides. (cdc.gov)
In this study, the effectiveness of polymerase chain reaction losis (Legionnaires disease) and can the water treatment protocol utilized (qRT-PCR) be found in soil and in aquatic environ- at Qatar University campus was exam- We extracted DNA from the water ments such as cooling towers ( 1,2 ). (who.int)
Investigations include smears, biopsy, staining with periodic acid-Schiff (PAS) or Gomori methenamine silver, culture of the affected tissues, polymerase chain reaction (PCR), serodiagnosis (sometimes), physical examination, and chest radiography. (medscape.com)

An initial denaturation of 30 seconds at 95°C is sufficient for most amplicons from pure DNA templates. (neb.com)
The antibodies dissociate during the initial PCR denaturation step and the DNA polymerase regains its full activity. (thermofisher.com)
Denaturation: The double-stranded DNA is heated up to 94 $$^o$$ C which causes the hydrogen bonds to break and the two strands get separated. (mcqexams.com)
Incomplete denaturation allows the DNA strand to snapback, reducing the product yield. (ojp.gov)

citation needed] The isolated enzyme has no intrinsic helicase activity but may carry out an equivalent function by way of its strong binding to single stranded DNA, particularly in preference to double stranded nucleic acid. (wikipedia.org)
The enzyme facilitates the "debranching" of double stranded DNA. (wikipedia.org)
No. The exonuclease will only degrade double stranded DNA that it encounters while extending a DNA fragment. (genscript.com)
DNA, a double-stranded molecule, is the primary genetic material directly responsible for development and inheritable traits. (aboutdarwin.com)
Two double-stranded DNA molecules. (aboutdarwin.com)
This enzyme unwinds the double-stranded DNA, paving the way for replication. (aboutdarwin.com)
Nick translation labeling is based on the reverse activities of Polymerase I and DNase I. DNase I is able to introduce randomly distributed nicks to double stranded DNA at low enzyme concentrations. (jenabioscience.com)
The well balanced polymerase / nuclease activities of the enzyme mix ensure the generation of highly labeled double stranded DNA fragments. (jenabioscience.com)
Finally, the double-stranded DNA is denatured by briefly heating the samples to 90 - 95°C. (ojp.gov)

Antibody modification increased specificity, sensitivity, and yield of PCR. (biolmedonline.com)
Meridian's High-Specificity Pfu HS Mix has higher sensitivity and yield over suppliers. (meridianlifescience.com)
This greatly enhanced the sensitivity of assays used to detect that specific sequence of DNA. (cdc.gov)
The novel protocol yielded the same sensitivity for drinking water biofilms associated with Fe3O4 (magnetite)-coated SiO2 (quartz) grains and biofilm-surface iron corrosion products from a drinking water distribution system. (who.int)

Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
Use only water and reagents that are free of DNA and nucleases. (biotechrabbit.com)

Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary Platinum antibodies. (thermofisher.com)
What was the first recombinant DNA based product, produced and marketed in India? (mcqexams.com)

Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. (neb.com)
Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment ( Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase. (neb.com)
Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. (nih.gov)
The blunt, phosphorylated ends were treated with Klenow fragment (3' to 5' exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. (nih.gov)
The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. (nih.gov)
Long-range PCR is a method to amplify the DNA fragment of more than 5Kb using a special type of high-fidelity DNA polymerase. (wazeesupperclub.com)
The efficiency of DNA labeling can be estimated by calculating the ratio of incorporated fluorophores to the number of bases in the fragment (dye / base). (jenabioscience.com)
Measure the absorbance of the labeled DNA fragment at 260 nm (A 260 ) and at the excitation maximum (λ exc ) for the dye (A dye ). (jenabioscience.com)
To test the TAQXpedite System with a routine template, a 539 bp fragment was amplified from 1, 10, and 100 pg of lambda DNA using a standard thermocycler. (genengnews.com)

The Expand High Fidelity PLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity. (sigmaaldrich.com)
Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. (gentaur.com)
Bst DNA Polymerase is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´→3´polymerase activity but lacks. (biolmedonline.com)

DNA ligase is used to link varying amounts of repeated structural units A and B and unique addressable unit X together into a long DNA segment, which can then be amplified by DNA polymerase to make many copies. (foresight.org)
The new method involves piecing together small strands one after the other, so that they attach into a longer DNA strand with the help of an enzyme known as ligase. (foresight.org)

The Expand High Fidelity PLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. (sigmaaldrich.com)
Can Taq DNA Polymerase or Green Taq be used to amplify GC-rich amplicons? (genscript.com)

Others FastStart Taq DNA Polymerase 100 U uL FastStart Taq DNA Polymerase, 100 U/μl 3.6.17.2.9.3 from Thermus aquaticus BM, expressed in E. coli, solution FastStart Taq DNA Polymerase, 100 U/uL custom fill CustomBiotech product. (roche.com)

Highly efficient DNA synthesis by the phage Φ29 DNA polymerase. (wikipedia.org)
On the other hand, RNA is a single chain of nucleotides primarily involved in protein synthesis following the master plan outlined by DNA. (aboutdarwin.com)
These two candidates were incorporated by solid-phase synthesis into DNA carrying the sequence of a promoter for an RNA polymerase enzyme. (chemistryviews.org)
The DNA turned out to be photoresponsive and allowed to modulate the rate of enzymatic RNA synthesis by light. (chemistryviews.org)
Eukaryotic DNA polymerase β (pol β), the polymerase thought to be responsible for DNA repair synthesis, has been extensively characterized in rats and humans. (biomedcentral.com)

The optional KB Extender used with Platinum Taq DNA Polymerase enables more versatility in PCR assays with long or GC-rich amplicons. (thermofisher.com)
The resultant DNA is suited for application in FISH, microarray gene expression profiling and other nucleic acid hybridization assays. (jenabioscience.com)
Anti- B burgdorferi assays commonly yield false-positive results because of cross-reactive antibodies associated with autoimmune diseases or with spirochetal rickettsial, ehrlichial, or bacterial (eg, Helicobacter pylori ) infections. (medscape.com)

Each 5' end is covalently linked to a terminal protein, which is essential in the replication process by acting as a primer for the viral DNA polymerase. (wikipedia.org)
In vitro experiments have shown that Φ29 replication can proceed to completion with the sole phage protein requirements of the polymerase and the terminal protein. (wikipedia.org)
The polymerase catalyses the formation of the initiation complex between the terminal protein and the chromosome ends at an adenine residue. (wikipedia.org)
The polymerase is a monomeric protein with two distinct functional domains. (wikipedia.org)
Poly(ADP-ribose) polymerase-1 (PARP-1) is an abundant and ubiquitous nuclear protein that uses NAD + to synthesize a multibranched polyanion composed of ADP-ribose moieties, giving rise to poly(ADP-ribose) (PAR), onto itself or a variety of target proteins. (nature.com)
Bst 2.0 WarmStart DNA Polymerase is prepared from an E. coli strain that expresses the Bst 2.0 DNA Polymerase protein from an inducible promoter. (neb.com)
Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. (nih.gov)
The search can specifically look for the DNA of the gene that produces the ricin protein. (cdc.gov)

It was also recently shown that PARP-1 is a sensor of unligated Okazaki fragments during DNA replication 16 and cells deficient in ribonucleotide excision repair are sensitized to PARP inhibition 17 . (nature.com)
The new fragments of DNA that are made during PCR also serve as templates to which the DNA polymerase enzyme can attach and start making DNA. (wazeesupperclub.com)
Place the tube in a precooled thermomixer at 15 °C. An incubation of 90 min is recommended to generate DNA fragments in a size range between 200 and 500 bp. (jenabioscience.com)
The Jena Bioscience PCR Purification Kit provides a simple and efficient way to purify DNA fragments larger than 100 bp. (jenabioscience.com)
Select the filter unit by its cut-off for DNA fragments and follow the manufacturer's instructions. (jenabioscience.com)

NZYTaq II DNA polymerase belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. (nzytech.com)
DNA polymerase to be used for standard PCR applications. (genaxxon.com)

Product Introduction This product is the Poly(A) Polymerase recombinantly expressed in E. coli. (biolmedonline.com)
The biofilm detection limit was 10(3) colony-forming units (CFU) E. coli K12 mL(-1), but required a modified protocol, which included a bio-blocker Pseudomonas aeruginosa with ethylenediaminetetraacetic acid buffered to pH 5 prior to cell lysis/DNA extraction. (who.int)
The novel DNA extraction protocol and specific E. coli K12 assay are sensitive and robust enough for detection and quantification within iron drinking water pipe biofilms, and are particularly well suited for studying enteric bacterial interactions within biofilms. (who.int)

The course plot for the saga of replication involves an array of characters, including enzymes like DNA polymerase, working tirelessly as diligent scribes, replicating each chapter accurately from the DNA template. (aboutdarwin.com)

Φ29 is a bacteriophage of Bacillus subtilis with a sequenced, linear, 19,285 base pair DNA genome. (wikipedia.org)

Obtaining uniform DNA yields from varying template concentrations is always important, but particularly essential for high-throughput applications, which require subsequent genetic analyses to be possible without additional measurement or adjustment of DNA concentration. (qiagen.com)
Here, we integrated isoform sequencing (iso-seq), Illumina short-reads sequencing and whole-genome bisulfite sequencing (WGBS) to explore the early changes of transcriptomic and DNA methylation in pineapple following high-concentration ethylene (HE) and low-concentration ethylene (LE) treatment. (nature.com)
The PCR (template) DNA must be a highly purified DNA having 30ng to 50ng concentration, 50% to 55% GC content and free from chemical contaminants and other DNA contaminants. (wazeesupperclub.com)
Diluting samples even 1:10,000 has been shown to be effective in improving results, depending on initial DNA concentration. (nzytech.com)
The optimum number of cycles depends mainly on the starting concentration of the target DNA when other parameters are optimized. (ojp.gov)

Briefly put, both DNA (Deoxyribonucleic Acid) and RNA (Ribonucleic Acid) carry genetic information of all life forms guiding their biological functions. (aboutdarwin.com)

this involves two replication origins and two distinct polymerase monomers. (wikipedia.org)
DNA Replication and RNA Transcription, although seemingly complex terms, hold within them concepts that are beautifully precise, methodical - true marvels of nature. (aboutdarwin.com)
Replication, in the world of genetics, is a mesmerizing process through which a cell makes an exact copy or, to draw a more precise picture - duplicates its DNA. (aboutdarwin.com)
It's quite an intricate dance aimed at making sure every new chromosome is equipped with one old and one new strand of DNA - this is referred to as semi-conservative replication. (aboutdarwin.com)
DNA replication is an intricate dance of molecules, a fundamental process essential to life. (aboutdarwin.com)
As we gaze into the microscopic world of the cell, here's a brief introduction to the components that are critical to the replication of DNA. (aboutdarwin.com)
b) DNA polymerase is an enzyme involved in DNA replication. (mcqexams.com)

The characteristics of its genome--a aplastic anemia, hemorrhage, and lymphoid depletion) and single, closed molecule of circular, negative-sense DNA increased death in young chicken ( 11 ). (cdc.gov)
It is a single standard DNA or RNA tagged with a radioactive molecule, which is complementary to the DNA in a clone of cells. (mcqexams.com)
a) The cell must be made competent in biotechnology experiments because DNA being a hydrophilic molecule, cannot pass through cell membrane. (mcqexams.com)
Hence, the cell should be made competent to accept the DNA molecule. (mcqexams.com)

pH 9.0 at +25°C ±0.1 Volume activity: ≥100 U/μL Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions. (roche.com)

It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone. (sigmaaldrich.com)

Green Taq DNA polymerase has terminal transferase activity that results in the addition of a single nucleotide (adenosine) at 3´-end of the extension product. (genscript.com)
In Polylox, random DNA recombination can be induced by transient activity of Cre recombinase in a 2.1-kb-long artificial recombination substrate that has been introduced into a defined locus in mice ( Rosa26Polylox reporter mice). (wazeesupperclub.com)
The former domain possesses a template-binding function and a dRPase activity to remove the 5'-deoxyribose phosphate, the latter domain possesses DNA polymerase activity. (biomedcentral.com)
RNA-dependent DNA polymerase activity of RNA tumor virus. (wikidata.org)

Can Taq DNA Polymerase be used in other buffers? (genscript.com)
Additionally, the Taq DNA polymerase can also work well in other buffers. (genscript.com)
The stabilizers in the buffers also increase the stability of Green Taq DNA polymerase at higher temperature (72℃), increasing the DNA yield of PCR amplifications of long DNA. (genscript.com)

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is a proofreading DNA polymerase which combines superior fidelity with trusted PlatinumTM hot-start technology for the highest success in PCR. (fishersci.com)

Two compounds with near-quantitative, fully reversible back-and-forth switching, high quantum yields, and high thermal and photochemical stability were identified. (chemistryviews.org)
The AF488 NT labeling mix contains specially optimized dUTP-XX-AF488 for incorporation into DNA by nick translation using DNA Polymerase I. The excellent stability and quantum yield of the fluorophore combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications. (jenabioscience.com)

Use of high quality, purified DNA templates greatly enhances the success of PCR. (neb.com)
The right enzyme can enhance the NGS library prep step - delivering greater yield, fidelity, and uniformity of coverage so that fewer total reads are needed, ending in high-quality data. (codexis.com)
Try using a high-fidelity DNA Polymerase. (genscript.com)
The enzyme was engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension) under minimal optimization conditions. (nzytech.com)
I-5 Hi-Fi DNA HotStart Polymerase is an ultra-fast and high-fidelity DNA polymerase. (carolinabiosystems.cz)
The syntheiss of high yields of full-length reverse transcripts of globin mRNA. (wikidata.org)
The kit contains the Expand High Fidelity DNA polymerase mix. (thomassci.com)
Ampliqon Taq DNA Polymerase is ideal for routine PCR applications where high yield and reliable. (genaxxon.com)

The dynamic turnover of PAR within seconds to minutes is executed by poly(ADP-ribose) glycohydrolase, the main PAR-degrading enzyme, which possesses both endoglycosidic and exoglycosidic activities, thereby enabling a new round of DNA damage signaling 14 . (nature.com)
Name the source organism that possesses Taq polymerase. (mcqexams.com)

Use this kit to label DNA with radioactive or nonradioactive nucleotides. (sigmaaldrich.com)
Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand at 72$$^0$$C. (mcqexams.com)
The 5'→3' exonuclease activitiy of Polymerase I removes nucleotides from the 3' side of the nick while synthesizing a partial new complementary strand using the 3'-OH termini as primer. (jenabioscience.com)

Because Taq DNA polymerase is active over a broad range of temperatures, primer extension will occur at low temperatures, including those used during the annealing step. (ojp.gov)

Sample Material can be supercoiled or linearized plasmid DNA, cosmid or BAC DNA, whole or partial chromosomes or purified PCR products. (jenabioscience.com)

This technology works with any DNA sequence and any vector. (genscript.com)
This substitution lies in a hairpin sequence within an 8-kDa domain, likely to be important in DNA binding. (biomedcentral.com)
In new research published May 5 in Nature Communications [ abstract ], however, Sleiman''s team at McGill reports that it has devised a technique to create much longer strands of DNA, including custom-designed sequence patterns. (foresight.org)
The polymerase process has the added advantage of correcting any errors that may have been introduced into the sequence, amplifying only the correctly sequenced, full-length product. (foresight.org)
In the end, what we get is a long, synthetic DNA strand with exactly the sequence of bases that we want, and with exactly as many repeat units as we want," explains Sleiman, who co-authored the study with Graham Hamblin, who recently completed his doctorate, and PhD student Janane Rahbani. (foresight.org)
Featuring >100x Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis and other applications benefiting from supreme sequence accuracy. (fishersci.com)

Evaluate amplified DNA by agarose gel electrophoresis followed by ethidium bromide staining. (wazeesupperclub.com)

Produces more DNA than PCR-based methods, by about an order of magnitude. (wikipedia.org)
PARP-1 acts as a highly sensitive sensor for DNA damage and rapidly produces PAR at newly generated DNA DSBs. (nature.com)

After sonication, histone-DNA complexes were immunoprecipitated with antibody. (nih.gov)

In this modified version of the standard PCR process, mRNA, which serves as the initial template, is first reversed transcribed to complementary DNA (cDNA) and then subsequently amplified via PCR for downstream analysis. (stratech.co.uk)

Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29. (wikipedia.org)

The new test extracts and analyzes DNA and RNA strands to detect sequences associated with viral and bacterial infections, Alere said. (medscape.com)

Maximizes product yield with Nano technology. (bio-helix.com)
Too many cycles can increase the amount and complexity of nonspecific background products (plateau effect) and too few cycles can produce low product yield. (ojp.gov)

Upon activation, PARP-1 synthesizes a structurally complex polymer composed of ADP-ribose units that facilitates local chromatin relaxation and the recruitment of DNA repair factors. (nature.com)
10 colony-forming units (CFU)/mL DNA extraction and critical ( 13 ). (who.int)

The Taq DNA Polymerase is intended for molecular biology applications. (qiagen.com)

The reactions were conducted in initiation of the study, had received no antiretroviral a 50-L PCR mixture containing Taq DNA polymerase, treatment. (cdc.gov)
Optionally, use the supplied 5X PCR Enhancer to increase the yield and to lower the background in more complicated PCR reactions (low amounts of template, impure or GC-rich template). (biotechrabbit.com)

The star enzyme that plays a critical role in synthesizing new DNA strands by following the older DNA as a template. (aboutdarwin.com)
Why is template DNA needed for PCR? (wazeesupperclub.com)

Our characterization of pol β from a model fish organism contributes to the study of the function and evolution of DNA polymerases, which are emerging as important cellular targets for chemical intervention in the development of anticancer agents. (biomedcentral.com)

In presence of dye-labeled dUTP Polymerase I incorporates labeled dUTP instead of dTTP. (jenabioscience.com)

Pol β is the smallest polymerase in family X. Other members of the family have a nuclear localization signal (NLS), a BRCA1 C-terminal (BRCT) domain, and a proline rich region. (biomedcentral.com)

Anatomy8

Organisms32

Diseases1

Chemicals and Drugs128

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes65

Information Science4

Health Care4

Amplification37

Genomic11

Primers14

Start Taq DNA Polyme7

Buffer11

Thermostable DNA polymerase4

DNTPs3

Exonuclease activity5

Reaction31

Denaturation4

Double stranded9

Sensitivity4

Reagents2

Recombinant2

Fragment9

Lacks3

Ligase2

Amplify2

Thermus1

Synthesis5

Assays3

Protein8

Fragments5

Standard PCR applications2

Coli3

Enzymes1

Bacillus1

Concentration5

Deoxyribonucleic Acid1

Replication7

Molecule4

Assay1

Amplifies1

Activity4

Buffers3

Invitrogen1

Stability2

High8

Possesses2

Nucleotides3

Primer1

Plasmid1

Sequence6

Agarose1

Produces2

Antibody1

Complementary1

Bacteriophage1

Viral1

Product yield2

Units2

Molecular1

Reactions2

Template2

Organism1

Incorporates1

Terminal1