• Toward the goal of understanding the functional significance of these subassemblies, we have used the gamma complex clamp loader and the beta ring to assemble each different polymerase onto DNA. (neb.com)
  • The addition of tau to the assembly reaction to form either core1-tau 2 or core2-tau 2 results in a more efficient polymerase and more stabile association of core-tau beta on DNA, although the gamma complex still does not remain on DNA. (neb.com)
  • The purified two active forms differed in chromatographic and electrophoretic behaviors, in their salt requirement for optimal activity, and in preference of template-primers, although both forms exhibited properties characteristic of DNA polymerase alpha such as sensitivity of N-ethylmaleimide, 1-beta-D-arabino-furanosylcytosine triphosphate, and aphidicolin. (nih.gov)
  • Aphidicolin, which inhibits both alpha- and delta-class enzymes, elicited the opposite pattern: DNA primers accumulated in its presence and were not converted into Okazaki pieces. (mysciencework.com)
  • Repairing these lesions is difficult because both DNA strands have been damaged by the chemical agent and thus the genetic information on both strands is incorrect. (wikipedia.org)
  • A β hairpin from the leading-strand polymerase separates two parental DNA strands into a T-shaped fork, thus enabling the closely coupled helicase to advance perpendicular to the downstream DNA duplex. (pdbj.org)
  • The clamp loader within Pol III* appeared to be capable of loading two beta clamps onto DNA for both core polymerases within Pol III*, consistent with the hypothesis that one replicase can simultaneously replicate both strands of a duplex chromosome. (neb.com)
  • Spt5 interacts with AID, it facilitates association between AID and Pol II, and AID recruitment to its Ig and non-Ig targets. (mdc-berlin.de)
  • The significance of these distinct polymerases in separate paths of DNA metabolism is discussed. (neb.com)
  • The high sensitivity of Platinum II Taq Hot-Start DNA Polymerase enables successful amplification of specific product in experiments where there is a limited amount of starting material or the target DNA is in low concentration in the sample. (thermofisher.com)
  • DNA polymerase I is heterogeneous comprising species covering a considerable range of molecular weights. (biochemj.org)
  • Sedimentation analyses on sucrose density gradients showed that the DNA polymerase I species had sedimentation coefficients predominantly in the range 6-8 S. DNA polymerase II had predominantly a sedimentation coefficient of 3.2 S although a component with lower sedimentation coefficient was found. (biochemj.org)
  • In several cases these are at different points in each organism, even though the DNA sequences surrounding the hotspots and their corresponding sites are very similar in both D. radiodurans and E. coli . (genetics.org)
  • Many of the bacterial members contain large insertions within this domain, which are known as dispensable region 2 (DRII). (ebi.ac.uk)
  • In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. (uniprot.org)
  • DNA polymerase I was found to be associated predominantly with the cytoplasm although certain types of nuclear preparation contained large amounts of it. (biochemj.org)
  • Deoxynucleoside triphosphate + DNA n = diphosphate + DNA n+1 . (rcsb.org)
  • Isolated nuclei contained two active forms of DNA polymerase alpha (form I and form II). (nih.gov)
  • Form II was extracted from nuclei by KCl at concentrations lower than 0.18 M. Above 0.18 M selective extraction of form I was observed. (nih.gov)
  • Marked difference between the two forms was preference of template-primer that form I was more active with poly(dT). (nih.gov)
  • PCR assays using conventional PCR reagents require specific protocols for amplification of each DNA fragment due to varying primer annealing temperatures and different durations of the extension step. (thermofisher.com)
  • With Platinum II Taq Hot-Start DNA Polymerase, different PCR assays can be cycled in parallel using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified. (thermofisher.com)
  • thus, the combination of this next-generation DNA polymerase and the universal protocol permits fast cycling of all assays in as little as 30 minutes. (thermofisher.com)
  • PCR assays using conventional PCR reagents require specific protocols for amplification of each DNA fragment because of the different primer annealing temperatures and extension steps. (thermofisher.com)
  • With Platinum II Taq Hot-Start DNA Polymerase, different PCR assays can be cycled together using one protocol with a universal primer annealing temperature and the extension step selected for the longest fragment to be amplified. (thermofisher.com)
  • Because the mutations that result in Rif r are clustered within two small regions of rpoB , they can be analyzed by using only two primer pairs for amplification and sequencing. (genetics.org)