Dna polymerase and primer. Medical search. Frequent questions

DNA polymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication. (wikipedia.org)
[ 8 ] As tHDA uses Bst DNA polymerase, an enzyme more tolerant to amplification inhibitors than the Taq polymerase used in PCR-based platforms, much simpler sample processing work-flows can be used in IsoAmp assays than in high-throughput molecular diagnostic systems relying on PCR. (medscape.com)
In step 1, the helicase enzyme loads on to the template and traverses along the target DNA, disrupting the hydrogen bonds linking the two strands. (medscape.com)
DNA joining is done by another enzyme called as ligase which forms continuous strands of DNA. (differencebetween.net)
The QIAGEN One-Step Ahead RT-PCR Kit contains a blend of Sensiscript and Omniscript Reverse Transcriptases, a well-balanced combination of Taq DNA Polymerases and a proofreading enzyme. (qiagen.com)
In this paper, site-directed mutants at this Lys residue were used to analyse its functional importance for the synthetic activities of φ29 DNA polymerase, an enzyme that starts linear φ29 DNA replication using a terminal protein (TP) as primer. (nih.gov)
The conservative amino acid change at residue 719 from alanine to valine results in a radical change in the properties of the polymerase, rendering the mutant enzyme resistant to PAA and various antiviral compounds. (microbiologyresearch.org)
Now enter our superstar molecule-Taq Polymerase-a heat-stable enzyme obtained from bacteria living in hot springs that synthesizes ("builds") new strands of DNA using nucleotides, starting at those primers. (aboutdarwin.com)
The enzyme catalyses DNA synthesis in a 5'→3' direction, shows no 3'→ 5' exonuclease activity, but has a 5'→ 3' exonuclease activity. (blirt.eu)
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction. (blirt.eu)
Utilizing an optimized enzyme to PCR amplify long and GC-rich templates, KOD Xtreme Hot Start DNA Polymerase successfully amplifies templates from crude or difficult to amplify samples, including plant tissues and mouse tail tips. (sigmaaldrich.com)

Replication factor C abrogates primer synthesis by DNA polymerase alpha at a critical length. (uzh.ch)
A crucial event in DNA replication is the polymerase switch from the synthesis of a short RNA/DNA primer by DNA polymerase alpha/primase to the pro?cessive elongation by DNA polymerase delta. (uzh.ch)
We show that RF-C stalls DNA polymerase alpha after synthesis of approximately 30 nucleotides, while not inhibiting the polymerase activity per se. (uzh.ch)
Thus we propose a model where RF-C, upon binding to the RNA/DNA primer, influences primer synthesis and sets the conditions for a polymerase switch after recruiting PCNA to DNA. (uzh.ch)
Members of this family have primase activity, catalyzing the synthesis of short RNA primers that serve as starting points for DNA synthesis, as well as DNA polymerase activity. (nih.gov)
The encoded protein facilitates DNA damage tolerance by mediating uninterrupted fork progression after UV irradiation and reinitiating DNA synthesis. (nih.gov)
Human PrimPol Discrimination against Dideoxynucleotides during Primer Synthesis. (nih.gov)
Structural basis of DNA synthesis opposite 8-oxoguanine by human PrimPol primase-polymerase. (nih.gov)
DNA replication is the process of DNA synthesis using parent DNA strands as a template. (online-sciences.com)
DNA replication is semiconservative: Each DNA strand serves as a template for synthesis of a new strand producing two DNA molecules , each with one new strand and one old strand. (online-sciences.com)
The RNA-primed synthesis of DNA demonstrating the template function of the complementary strand parental DNA . (online-sciences.com)
The synthesis of a DNA molecule can be divided into three stages: initiation, elongation, and termination. (online-sciences.com)
HDA uses only two oligonucleotide primers to initiate the exponential DNA synthesis needed to reach high analytical sensitivity. (medscape.com)
Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
The main function of DNA polymerase 1 is DNA labeling by nick translation and second strand synthesis of cDNA. (differencebetween.net)
DNA polymerase 1 also catalyzes 5' to 3' synthesis of DNA. (differencebetween.net)
Synthesis of herpes simplex virus, vaccinia virus and adenovirus DNA in isolated HeLa cell nuclei. (microbiologyresearch.org)
It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. (blirt.eu)
The Y-family DNA polymerases lie at the heart of this process, which is commonly known as translesion synthesis. (nih.gov)
The enzymatic non-radioactive labelling of oligonucleotides has become a valuable tool in applications such as oligonucleotide hybridisation probe synthesis and automated DNA sequencing. (soton.ac.uk)

DNA polymerase III synthesizes base pairs at a rate of around 1000 nucleotides per second. (wikipedia.org)
Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
DNA polymerases are specially designed enzymes which help in formation of DNA molecules by assembling tiny building blocks of DNA called as nucleotides . (differencebetween.net)
The fact that must be remembered is that this DNA polymerase 1 only adds the nucleotides but does not join them. (differencebetween.net)
DNA polymerase 3 is essential for the replication of the leading and the lagging strands whereas DNA polymerase 1 is essential for removing of the RNA primers from the fragments and replacing it with the required nucleotides. (differencebetween.net)

This gene encodes a DNA primase-polymerase that belongs to a superfamily of archaeao-eukaryotic primases. (nih.gov)

Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. (sigmaaldrich.com)
PCR reactions require several critical reagents, including the template sequence for amplification, dNTPS, buffers, and Taq DNA polymerase to enzymatically facilitate the incorporation of dNTPs and ultimately create new copies of your target sequence. (sigmaaldrich.com)
The basic principle of Hot Start PCR methods and reagents is that they are designed to inhibit Hot Start Taq DNA polymerase activity, or the incorporation modified dNTPs during reaction set up until a heat activation step occurs. (sigmaaldrich.com)
Additionally, our KOD Hot Start Master Mix provides an all-in-one solution by combining KOD Hot Start DNA Polymerase, dNTPs, and optimized reaction buffer in a 2X ready-to-use mixture. (sigmaaldrich.com)
Add a cocktail that has the dna polymerase and dNTPs. (ostatic.com)
Every time you go through the steps, do not need to add more dNTPS, but need to add more polymerase because they denature. (ostatic.com)

DNA polymerase 1 has 3 activities like polymerase, 3' to 5' exonuclease and 5' to 3' exonuclease. (differencebetween.net)
The alpha subunit is responsible for the DNA polymerase activity, the epsilon subunit has proof reading exonuclease activity and the theta subunit is the smallest of all and helps in enhancing the proof reading properties of epsilon. (differencebetween.net)
The high-fidelity proofreading DNA Polymerase with 3'→5' exonuclease activity is also heat-activated by the initial 5-minute incubation step at 95°C, ensuring superior amplification accuracy and processivity. (qiagen.com)
The KOD Hot Start DNA Polymerase is a premixed complex of KOD DNA polymerase that utilizes two highly specific monoclonal antibodies to inhibit 3′→5′ exonuclease activity at room temperature. (sigmaaldrich.com)

They named the method the polymerase chain reaction (PCR). (nih.gov)
1. Inoshima Y, Morooka A, Sentsui H. Detection and diagnosis of parapoxvirus by the polymerase chain reaction. (cdc.gov)
Specific ERα and ERβ inhibitors and real-time polymerase chain reaction were used to identify potential receptor(s) that mediate the actions of BPA. (nih.gov)
The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)
[ 1 ] The resultant complementary DNA is amplified by polymerase chain reaction (PCR). (medscape.com)
After DNA extraction, polymerase chain reaction (PCR) was carried out using specific primers for the targeted mitochondrial genome. (researchgate.net)
One such revolutionary technique is the Polymerase Chain Reaction, commonly known as PCR. (aboutdarwin.com)
The Polymerase Chain Reaction (PCR) is a fast and inexpensive technique used to "amplify" - copy-specific segments of DNA. (aboutdarwin.com)
Why is Polymerase Chain Reaction Important? (aboutdarwin.com)
2. Nested polymerase chain reaction-based HLA class II typing for the unique identification of formalin-fixed and paraffin-embedded tissue. (nih.gov)
9. A novel HLA-A*02 allele, A*02:543 was identified by polymerase chain reaction sequence-based typing in a Chinese cord blood donor. (nih.gov)
18. [DNA typing for HLA-A by polymerase chain reaction with sequence-specific primers]. (nih.gov)
Sample analysis: Real-time polymerase chain reaction (qPCR) with universal primer to quantify DNA copy of microbial agents. (cdc.gov)

The advent of thermostable polymerases from thermophilic bacteria and archaea and computer-controlled thermocycling machines have made PCR a standard laboratory technique that is used in a wide range of molecular biology procedures including measuring gene expression, amplifying targets for sequencing and cloning, and assaying sequence variation. (nih.gov)
In PCR, a thermostable DNA polymerase is used to amplify target DNA 2-fold with each temperature cycle. (medscape.com)
TaqNova DNA Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus . (blirt.eu)
Additionally, the FastStart™ High Fidelity PCR System utilizes a blend of FastStart™ Taq DNA Polymerase and a chemically modified, thermostable proofreading protein. (sigmaaldrich.com)

Our results show that single replacement of φ29 DNA polymerase residue Lys529 by Ala or Glu decreases the stabilisation of the primer-terminus at the polymerisation active site, impairing both the insertion of the incoming nucleotide when DNA and TP are used as primers and the translocation step required for the next incoming nucleotide incorporation. (nih.gov)
By comparative sequence analysis of the herpes simplex virus type 1 DNA polymerase gene of strain Angelotti and a phosphonoacetic acid-resistant (PAA r ) derivative, the site of the PAA r mutation was identified as a single nucleotide (C → T) conversion within the mapping limits of the known PAA r mutations of strains KOS and 17. (microbiologyresearch.org)
Homology between DNA polymerases of poxviruses, herpesviruses, and adenoviruses: nucleotide sequence of the vaccinia virus DNA polymerase gene. (microbiologyresearch.org)
The RNA polymerase is more likely to misincorporate a nucleotide at premature stop codons. (biorxiv.org)
However, stop codon miscoding (SCM) may occur either by a transcription error, when the RNA polymerase misincorporates a nucleotide and eliminates the stop codon, or by a translation error when the ribosome misincorporates a tRNA at the stop codon (also called stop codon readthrough and nonsense suppression 5 , 6 ). (biorxiv.org)
The enzymatic incorporation of nucleotide analogues into oligonucleotides by Taq DNA polymerase has been investigated using linear primer extension assays and PCR experiments. (soton.ac.uk)
DNA sequence analysis of inherited cataract families showed 72 nucleotide variations, of which 15 were observed in the major non-coding D-loop region, 3 in the tRNA genes, 5 in the rRNA genes, and 49 in the protein coding region. (molvis.org)

The amplification step features a OneTaq Hot Start DNA Polymerase in a master mix format. (neb.com)
OneTaq Hot Start DNA Polymerase offers higher fidelity than Taq and better amplification. (neb.com)
Eliminating mispriming and primer-dimer formation, KOD Hot Start DNA Polymerase is an ideal reagent for long strand amplification of genomic DNA templates (up to 12 kb), plasmid and lambda DNA templates (up to 21 kb). (sigmaaldrich.com)
The figure on the left demonstrates the genomic DNA amplification using KOD Hot Start DNA Polymerase of human myosin heavy chain gene (8.4 kb) and human β-globin gene (12.3 kb). (sigmaaldrich.com)
The figure on the right indicates lambda DNA was amplified using appropriate primers and KOD Hot Start DNA Polymerase. (sigmaaldrich.com)

This high processivity is due in part to the β-clamps that "hold" onto the DNA strands. (wikipedia.org)
In addition, by using a different ratio of the two amplification primers, HDA can yield a surplus of one of the two strands of DNA, such as to allow for the use of hybridization probes to confirm the legitimacy of the NAAT amplification products without a denaturation heating step. (medscape.com)
The temperature of these is increased to 90-95°C in order to separate (denature) the 2 strands of target DNA. (medscape.com)
DNA polymerase helps in reading the already existing DNA strands to create two new strands that match the original existing DNA. (differencebetween.net)
Firstly, it involves heating up our genetic material to separate the two strands of DNA-we call this denaturing . (aboutdarwin.com)

The last item, specificity, requires searching the primers against a database of background DNA sequences to look for unintended matches. (nih.gov)
Phylogenetic reconstruction was carried out with data from DNA sequences using the maximum parsimony and Bayesian inference criteria to explore the monophyly of the genus, its subgenera and its position within Hylocereeae. (researchgate.net)
Citation: Cruz M. Á, Arias S. & Terrazas T. 2016: Molecular phylogeny and taxonomy of the genus Disocactus (Cactaceae), based on the DNA sequences of six chloroplast markers. (researchgate.net)
Nested PCR utilizes two sets of primers, the first set flank the sequences directly outside the target sequence for the first round of amplification. (sigmaaldrich.com)
Multiplex PCR utilizes multiple sets of highly specific primers to amplify multiple target sequences simultaneously during the same reaction. (sigmaaldrich.com)
TaKaRa LA Taq DNA Polymerase with GC Buffer has the Takara LA Taq DNA Polymerase blend for long and accurate PCR paired with GC-optimized buffers to power through difficult-to-amplify GC-rich sequences, including genomic DNA. (takarabio.com)
Heat it up (typically 95 degrees Celsius) Add primer find a sequence to bind to (short sequences). (ostatic.com)

The PCR assay, based on degenerate primers targeting highly conserved regions of the DNA polymerase gene of herpesviruses, was modified by using a mixture of degenerate and deoxyinosine-substituted primers. (nih.gov)
the α subunit (encoded by the dnaE gene) has the polymerase activity. (wikipedia.org)
DNA sequence analysis of the region encoding the terminal protein and the hypothetical N-gene product of adenovirus type 2. (microbiologyresearch.org)
Mutations in the herpes simplex virus DNA polymerase gene can confer resistance to 9- β - d -arabinofuranosyladenine. (microbiologyresearch.org)
Human genomic DNA was used to amplify a 4.8 kb fragment tPA gene. (sigmaaldrich.com)
A 2.1 kb mouse rRNA gene (63.4% GC) was amplified from of mouse genomic DNA. (takarabio.com)
The HD gene is GC-rich and highly repetitive and the primers used in this experiment amplified regions of the HD gene IT15 CAG repeat. (takarabio.com)

In the mid-1980's Kary Mullis and others devised a method to rapidly amplify a targeted region of DNA into milliions of copies. (nih.gov)
It allows us to amplify minimal amounts of DNA and obtain millions or billions of copies in just a few hours. (aboutdarwin.com)
For the longest amplicons that are also GC rich, try PrimeSTAR GXL DNA Polymerase , which is designed to amplify genomic DNA up to 30 kb. (takarabio.com)

The replisome is composed of the following: 2 DNA Pol III enzymes, each comprising α, ε and θ subunits. (wikipedia.org)
DNA polymerase I & III, along with many other enzymes are all required for the high fidelity, high-processivity of DNA replication. (wikipedia.org)
Other enzymes (e.g. helicase, topoisomerase, and DNA ligase) and protein factors (e.g. origin binding proteins and single-stranded binding proteins) are required for the replication process. (online-sciences.com)
After incubation, the reaction mixture is transferred to the Amplification Microwell, which contains two enzymes (a DNA polymerase and a restriction endonuclease) necessary for SDA. (cdc.gov)
Similar experiments are used to identify human DNA repair enzymes that remove specific forms of DNA damage. (vanderbilt.edu)

1 γ unit (also dnaX) which acts as a clamp loader for the lagging strand Okazaki fragments, helping the two β subunits to form a unit and bind to DNA. (wikipedia.org)
DNA Pol III activity begins after strand separation at the origin of replication. (wikipedia.org)
The removal of the RNA primer allows DNA ligase to ligate the DNA-DNA nick between the new fragment and the previous strand. (wikipedia.org)
The free 3´ hydroxyl group of the primer acts as an accepter for the first deoxyribonucleotide in the newly formed DNA strand. (online-sciences.com)
DNA polymerases utilize one deoxyribonucleoside triphosphate as a source of the deoxyribonucleoside monophosphate for the growing DNA strand by the removal of pyrophosphate. (online-sciences.com)
DNA polymerase ε removes the primers of Okazaki fragments on the lagging strand. (online-sciences.com)
During the process of replication, an RNA primer is filled in the lagging strand of the DNA. (differencebetween.net)
Then comes annealing , where short pieces of DNA called primers attach themselves to specific sections on each strand. (aboutdarwin.com)
DNA polymerase uses the single strand as a template and makes copies of the complementary strand. (ostatic.com)
DNA strand. (ostatic.com)
Polymerase attaches to the strand and starts making a new strand of DNA (in the reverse direction). (ostatic.com)

Finally PCNA can be loaded onto a DNA template carrying a RNA primer, suggesting that a DNA moiety is not necessarily required for the loading of the clamp. (uzh.ch)
DNA polymerases require the presence of a primer (i.e. oligonucleotide of RNA with free 3´ hydroxyl group), a template (i.e single-stranded DNA), and deoxyribonucleotides (d ATP , d CTP, d GTP, and d TTP) in order to function. (online-sciences.com)
The CIC is a spike-in-template DNA that shares the same primer binding sequence as the target sequence, but has a different internal sequence such that it can be detected with a separate probe in BioHelix-designed NAAT. (medscape.com)
Delavirdine does not compete with template: primer or deoxynucleoside triphosphates. (nih.gov)
DNA polymerase 1 is a template dependent DNA polymerase. (differencebetween.net)
The second set of primers are specific for the target sequence from the first round, which is then used as the template for the second round of PCR using the nested primers. (sigmaaldrich.com)
For all reactions, purified human genomic DNA (100 ng in a 50 µL reaction) was used as a template in reactions containing Takara LA Taq polymerase with either LA PCR Buffer II (lane 1), GC Buffer I (lane 2), or GC Buffer II (lane 3), or a competitor's high GC DNA polymerase kit (lane 4). (takarabio.com)

DnaX complex of Escherichia coli DNA polymerase III holoenzyme. (wikipedia.org)

The cartoon depicts bacterial DNA polymerases: replicative holoenzyme pol III [yellow] and three SOS-inducible polymerases, pol II (blue), pol IV (green), and pol V (violet/orange). (nih.gov)
DNA polymerase 3 is also called as holoenzyme and it is the most essential component of replisome. (differencebetween.net)

Using the OmpR protein, we identify residues in the flexible linker and the C-terminal β-hairpin that enable associations of this archetypical response regulator with thioredoxin, but are dispensable for interactions of this transcription factor to its cognate sensor kinase EnvZ, DNA or RNA polymerase. (nature.com)
Both the FastStart™ polymerase and proofreading protein are inactive below 75 °C and activated by the removal of blocking groups after being heated to 95 °C for just two minutes. (sigmaaldrich.com)

(A) The main replicase, pol III, is engaged at the primer terminus. (nih.gov)
(B) At a DNA lesion, pol III disengages from the primer terminus and TLS polymerase rotates into position so as to promote TLS. (nih.gov)
Resolution of the crystallographic structure of φ29 DNA polymerase binary and ternary complexes showed that residue Lys529, located at the C-terminus of the palm subdomain, establishes contacts with the 3' terminal phosphodiester bond. (nih.gov)
The replicative DNA polymerase stalls at a site of DNA damage (red `H'), then a TLS polymerase (or polymerases) is recruited to the primer terminus and correct, or incorrect, bases are inserted opposite the lesion. (nih.gov)
All TLS polymerases are distributive and dissociate from the primer terminus after synthesizing a short tract of DNA. (nih.gov)

Mix 30 µl DNA with 10 µl 4X tris/EDTA/sucrose loading buffer (does not contain dyes). (nih.gov)
10 x TaqNova (NH 4 ) 2 SO 4 buffer is recommended for applications where high sensitivity and amplification efficiency is required (e.g. for amplification of multiple DNA fragments). (blirt.eu)

DNA Pol III is a component of the replisome, which is located at the replication fork. (wikipedia.org)
DNA polymerase 3 is a component of the replisome and hence helps in replication. (differencebetween.net)

QuantiNova DNA Polymerase is provided in an antibody-mediated, inactive state and has no enzymatic activity at ambient temperature. (qiagen.com)
Various methods are available to halt Hot Start polymerase activity and include, chemical modification, antibody-mediated, and aptamer-mediated technology. (sigmaaldrich.com)

Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
Contamination of reagents or specimens with target DNA from the environment must be avoided because such contamination can produce false-positive results. (medscape.com)
The processed sample is added to the Priming Microwell, which contains the amplification primers, fluorescent labeled detector probe, and other reagents necessary for amplification. (cdc.gov)
The kit provides all necessary reagents optimized for rapid and sensitive detection of a low percentage of mutant DNA in a background of wild-type genomic DNA. (qiagen.com)

Exposure of the single-stranded target region by helicase allows primers to anneal. (medscape.com)
Topoisomerase relaxes the coil and Helicase unzips the DNA. (ostatic.com)

They are then quickly replaced by the cell's replicative polymerase, which completes genome duplication. (nih.gov)

Use of Pol I-deficient E. coli for functional complementation of DNA polymerase. (nih.gov)
RÉSUMÉ Les souches d'Escherichia coli diarrh￩og￨nes peuvent ￪tre consid￩r￩es comme les agents ￩tiologiques les plus importants ￠ l'origine de diarrh￩es en R￩publique islamique d'Iran, notamment chez l'enfant. (who.int)

Band shift experiments indicated that RF-C has a preference for 5' recessed ends and double-stranded DNA over 3' ends. (uzh.ch)
Standard PCR uses 30-40 cycles of denaturing a double-stranded target DNA at high temperature, lowering the temperature to allow the primers to bind and the polymerase to extend the primers. (nih.gov)
The polymerase latches onto a replication fork where a double stranded dna is exposed. (ostatic.com)

The BDProbeTec CT Chlamydia trachomatis Amplified DNA Assays are based on the simultaneous amplification and detection of target DNA, using amplification primers and a fluorescent labeled detector probe. (cdc.gov)

A consensus primer PCR approach was used to (i) investigate the presence of herpesviruses in wild and zoo equids (zebra, wild ass, tapir) and to (ii) study the genetic relationship of the herpesvirus of pigeons (columbid herpesvirus 1) to other herpesvirus species. (nih.gov)
The applicability of the modification was validated by amplification of published DNA polymerase genes of 16 herpesvirus species and of the previously uncharacterized DNA polymerase genes of equine herpesvirus 3 (EHV-3) and equine herpesvirus 5 (EHV-5). (nih.gov)
The modified assay was then used for partial amplification of the polymerase of columbid herpesvirus 1 which is presently classified as a beta-herpesvirus based on biological criteria. (nih.gov)
This was confirmed by characterization of additional 1.6kb of the columbid herpesvirus 1 polymerase. (nih.gov)
Mutally exclusive inhibition of herpesvirus DNA polymerase by aphidicolin, phosphonoformate, and acyclic nucleoside triphosphate. (microbiologyresearch.org)

Structures of phi29 DNA polymerase complexed with substrate: the mechanism of translocation in B-family polymerases. (neb.com)

Samples of mtDNA were amplified using 24 pairs of overlapping primers to analyze the entire mitochondrial genome to screen for variations and analyzed for both coding and non-coding regions. (molvis.org)

RNA polymerase transcribes genes encoding downstream adaptive outputs. (nature.com)
19. High and intermediate resolution DNA typing systems for class I HLA-A, B, C genes by hybridization with sequence-specific oligonucleotide probes (SSOP). (nih.gov)

Amino acid homologies as well as secondary structure analysis reveal that the PAA r mutation is contained in a 14 amino acid sequence which is highly conserved, and detected in the central domain of prokaryotic and eukaryotic DNA polymerases. (microbiologyresearch.org)

It starts with a DNA sample, which contains the segment you want to replicate (Target DNA). (aboutdarwin.com)
The past 15 years have seen an explosion in our understanding of how cells replicate damaged DNA and how this can lead to mutagenesis. (nih.gov)

DNA polymerase 3 is essential for pro-karyotic DNA replication and was discovered by Thomas Kornberg and Malcolm Gefter. (differencebetween.net)

14. Complete HLA-A DNA typing using the PCR-RFLP method combined with allele group- and sequence-specific amplification. (nih.gov)

[ 6 ] The probe-amplicon complex formed by the hybridization of the probe to the biotin-labeled single-stranded DNA is detected through sandwich immunoassays using anti-FITC, anti-DIG and anti-DNP antibodies striped onto a vertical flow test strip, and a streptavidin-latex conjugate applied to the test strip. (medscape.com)
THE SANGER METHOD:Single-stranded DNA is mixed with a primer and split into four aliquots, each containing DNA polymerase, four deoxyribonucleotide triphos-phates and a replication terminator. (the-scientist.com)

It has DNA repair and proofreading activities. (online-sciences.com)

By enabling the amplification of DNA fragments, PCR plays an essential role in genetic studies like genotyping, mutation detection, and sequencing. (aboutdarwin.com)
11. Detection of HLA-A*24 null alleles by DNA typing methods. (nih.gov)

The drive to actually set up new high-throughput genome centers to carry out high-throughput sequencing is not there to the same degree as it was when the drive was on to sequence the human genome," says Andy Bertera, vice president of core products at GE Healthcare, the second largest supplier of DNA sequencers. (the-scientist.com)
DNA sequence and expression of the B95-8 Epstein-Barr virus genome. (microbiologyresearch.org)
In simpler words, if we consider DNA as an individual recipe within a cookbook (genome), PCR allows us to make multiple copies of that particular recipe quickly. (aboutdarwin.com)

DNA polymerase 1 reads the shape and polarity of the incoming dNTP. (differencebetween.net)

Having multiple origins of replication provides a mechanism for rapidly replicating the great length of eukaryotic DNA molecules. (online-sciences.com)
DNA replication occurs in the nucleus during the synthetic (S) phase of the eukaryotic cell cycle . (online-sciences.com)

Both reaction buffers provided may be used with TaqNova DNA polymerase. (blirt.eu)
RR02AG is Takara LA Taq DNA polymerase paired with two special GC-optimized Buffers. (takarabio.com)

Repeated cycles result in an exponential growth of the number of copies of the target DNA. (nih.gov)
Whereas conventional PCR is often utilized to make exponential copies of your DNA target sequence without an additional temperature-sensitive reaction activation component. (sigmaaldrich.com)

The PCR amplification step is catalyzed by a well-balanced blend of three different DNA polymerases. (qiagen.com)

Scorpions are bifunctional molecules containing a PCR primer covalently linked to a probe. (qiagen.com)

Isolate DNA using the Costello lab's "genomic DNA extraction from frozen tissue" protocol. (nih.gov)
16. Evaluation of DNA extraction methods and real time PCR optimization on formalin-fixed paraffin-embedded tissues. (nih.gov)

However, as they exhibit low fidelity when copying undamaged DNA, it is essential that they are only called into play when they are absolutely required. (nih.gov)

In addition, combination of the DNA polymerase mutants with a TP derivative at residue Glu233, neighbour to the priming residue Ser232, leads us to infer a direct contact between Lys529 and Glu233 for initiation of TP-DNA replication. (nih.gov)
Acyclovir-resistant mutants of herpes simplex virus type 1 express altered DNA polymerase or reduced acyclovir phosphorylating activities. (microbiologyresearch.org)

Additionally, AZT had no effect on DNA polymerase activity and did not cause cell damage in vitro and in vivo . (jneurosci.org)
Wilson, S.H. and Kuff, E.L. A novel DNA polymerase activity found in association with intracisternal A-type particles. (nih.gov)

Follow the Illumina Genomic DNA kit protocol, which uses 10 µl adapter oligo mix for 5 µg starting DNA. (nih.gov)
12. Comprehensive method for the typing of HLA-A, B, and C alleles by direct sequencing of PCR products obtained from genomic DNA. (nih.gov)
Total genomic DNA from soil samples from six locations and tuber samples from four potato cultivars (Spunta, Shepody, Innovator and Russet Burbank) were assessed. (elsevier.es)

2 β units (dnaN) which act as sliding DNA clamps, they keep the polymerase bound to the DNA. (wikipedia.org)
Two DNA polymerases are shown simultaneously bound to the dimeric β-clamp. (nih.gov)

Computer programs such as PrimerSelect™ and Primer3 can be used to design or analyze primers. (neb.com)

It's remarkable that the first experiments used a manual process involving water baths and a polymerase that was unstable at high temperatures. (nih.gov)

The benefits of Hot Start PCR include convenient room temperature set up without nonspecific amplification and the formation of primer-dimers, both of which can reduce target sequence specificity and overall yield. (sigmaaldrich.com)

The kit has specifically designed primers combined with composite fluorescent probes, thereby allowing utilization of realtime fluorescent PCR. (aurora-instr.com)

Anatomy7

Organisms32

Chemicals and Drugs124

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes67

Information Science4

Health Care4

Enzyme11

Synthesis20

Nucleotides6

Primase1

DNTPs6

Exonuclease4

Chain reaction13

Thermostable4

Nucleotide7

Start DNA Polymerase5

Strands5

Sequences7

Gene7

Amplify3

Enzymes5

Strand11

Template7

Escherichia1

Holoenzyme2

Protein2

Terminus5

Buffer2

Component of the replisome2

Antibody-mediated2

Reagents4

Helicase2

Replicative1

Coli2

Double-stranded3

Assays1

Herpesvirus5

Translocation1

Mitochondrial genome1

Genes2

Prokaryotic1

Replicate2

Thomas Kornberg1

Sequence-specific1

Single-stranded2

Proofreading1

Detection2

Genome3

DNTP1

Eukaryotic2

Buffers2

Exponential2

Blend1

Probe1

Extraction2

Fidelity1

Mutants2

Activity2

Genomic DNA3

Bound2

Analyze1

High temperatures1

Target sequence1

Fluorescent1