• NEB also offers various conventional double-stranded DNA molecular weight markers with sizes up to 23 kb and markers for pulsed-field gel electrophoresis (PFGE) ranging from 1.5 to 1,018 kb. (neb.com)
  • A variety of Pulsed Field Gel Electrophoresis profiles was observed by macrorestriction DNA analysis in S. epidermidis isolates, but two of three S. haemolyticus isolates presented the same profile. (scielo.br)
  • Diferentes perfis eletroforéticos obtidos pela técnica de "Pulsed Field Gel Electrophoresis" foram observados na análise da macrorestrição do DNA nos isolados de S. epidermidis, mas dois dos três isolados de S. haemolyticus apresentaram o mesmo perfil. (scielo.br)
  • hQ-NEW-50 bp+ DNA ladder is convenient for sizing of double-stranded DNA on agarose gels. (biosan-nsk.ru)
  • The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer. (neb.com)
  • Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. (lookformedical.com)
  • The enzyme has a preference for double-stranded DNA. (lookformedical.com)
  • Incoming partly double stranded hepatitis B virus (HBV) DNA genomes are completed by the viral polymerase generating relaxed circular DNA (rcDNA). (biomedcentral.com)
  • 100bp Plus DNA Ladder is a premixed, ready-to-load molecular weight marker containing eight linear double-stranded DNA fragments. (transgenbiotech.com)
  • The DNA ladder consists of 13 DNA fragments from 50 to 1500 bp. (biosan-nsk.ru)
  • Molecular weight ladders are used to estimate the size of DNA and protein fragments by comparing their migration distance to that of the colored bands. (wikipedia.org)
  • Agarose gel analysis is the most commonly used method for analyzing DNA fragments between 0.1 and 25 kb, while pulse-field gel electrophoresis enables analysis of DNA fragments up to 10,000 kb. (qiagen.com)
  • The concentration of agarose used for the gel depends primarily on the size of the DNA fragments to be analyzed. (qiagen.com)
  • Low agarose concentrations are used to separate large DNA fragments, while high agarose concentrations allow resolution of small DNA fragments (see table Concentration of agarose used for separating fragments of different sizes ). (qiagen.com)
  • The drawback of TBE is that the borate ions in the buffer form complexes with the cis-diol groups of sugar monomers and polymers, making it difficult to extract DNA fragments from TBE gels using traditional methods. (qiagen.com)
  • The digested DNA includes fragments ranging from 100 bp to 10 kb. (neb.com)
  • Sequencing and further analysis of the HDP2 DNA fragments of 19 Asiatic isolates of T. saginata and T. asiatica indicated that the HDP2 sequences of both species exhibited clear genomic variability, due to polymorphic variable fragments, that could correspond to the non-transcribed region of ribosomal DNA. (biomedcentral.com)
  • The payoffs are good resolution of small DNA fragments (~ 100 bp - 3 kb) and time saved with a shorter gel run. (bitesizebio.com)
  • One big drawback that SB and LB buffers share with their cousin, TBE, is poor resolution of large DNA fragments. (bitesizebio.com)
  • Lithium acetate (LA) buffer is the voltage-cranking equivalent to TAE, allowing quick resolution of large DNA fragments. (bitesizebio.com)
  • This became my solution for gel-purification of vectors, but the downside here is poor resolution of small DNA fragments. (bitesizebio.com)
  • My DNA digested into fragments. (bitesizebio.com)
  • There is however, a potentially confounding issue in that PCR can generate larger targets from collections of small DNA fragments, a phenomenon known as PCR recombination. (biomedcentral.com)
  • The impact of PCR recombination towards the amplification of this cccDNA specific target was explored by mixing three marked, yet overlapping HBV DNA fragments. (biomedcentral.com)
  • Similarly "DNA shuffling" generates large recombinant DNA molecules from smaller DNA fragments through template switching [ 7 ]. (biomedcentral.com)
  • Primers incorporated mutations (underlined) to distinguish PCR fragments from input HBVayw DNA. (biomedcentral.com)
  • The separation is not as good as PAGE electrophoresis, which can distinguish DNA fragments that differ by 1bp. (yeasenbiotech.com)
  • The DNA Ladder contains 100bp -1.5 kb DNA fragments. (transgenbiotech.com)
  • Genomic DNA can be isolated directly from cells immobilized in low-melt agarose gels (see reference 6 for more information). (qiagen.com)
  • Bacteria DNA Extraction Kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). (parstous.com)
  • Purified genomic DNA is of an excellent quality and yield. (parstous.com)
  • However, many times genomic DNA from these samples is limited, which may hinder the researcher's ability to perform downstream analysis. (sigmaaldrich.com)
  • This protocol provides a simple and convenient method to extract genomic DNA from a blood card. (sigmaaldrich.com)
  • The GenElute Blood Genomic DNA Kit (Product No. NA2000 ) is recommended for this procedure. (sigmaaldrich.com)
  • The products amplified using GenomePlex technology were of a smaller molecular weight as shown on the gel when compared to Supplier A and Q. This is due to the random fragmentation of genomic DNA prior to amplification. (sigmaaldrich.com)
  • Sigma's amplified products are specific and there is no amplicon visible in the negative control (lane 2) indicating that only the desired genomic DNA is amplified. (sigmaaldrich.com)
  • Danio rerio (zebrafish) has long been used as a model organism in the fields of molecular genetics and developmental biology of vertebrates [ 13 ]. (biomedcentral.com)
  • In this study, molecular biology methods and phylogenetic studies were combined with drug assays. (biomedcentral.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • Eijkman Institute for Molecular Biology Jl. (who.int)
  • and (i i) to develop (epi)genomic powerful molecular and/or cell biology and close col aboration to create synergies methodologies, profiling strategies, and functional genomics tools, recent progress and better exploit and further expand bioinformatics tools and resources that in understanding of the cancer (epi) unique research tools and expertise. (who.int)
  • We would like to offer the pPSU1 & pPSU2 pair of plasmids as an alternative to commercial or other homemade sources of DNA ladders. (plantae.org)
  • NEB offers a variety of DNA ladders with sizes ranging from 10 bp to 48.5 kb for use in DNA gel electrophoresis, including standard agarose and polyacrylamide (PAGE) electrophoresis. (neb.com)
  • NEB DNA ladders are available in standard formats and in a variety of convenient, ready-to-load formats. (neb.com)
  • 1 kb Plus DNA Ladder for Safe Stains is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. (neb.com)
  • 1 kb Plus DNA Ladder for Safe Stains consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. (neb.com)
  • 1 kb Plus DNA Ladder for Safe Stains visualized on a 1.0% TBE agarose gel precast with GelRed. (neb.com)
  • We recommend loading 5 µl (0.25 µg) of 1 kb Plus DNA Ladder for Safe Stains per gel lane on GelRed ® and GelGreen ® precast agarose gels. (neb.com)
  • We recommend loading 10 µl (0.5 µg) of 1 kb Plus DNA Ladder for Safe Stains per gel lane on SYBR ® Safe precast agarose gels. (neb.com)
  • 1 kb Plus DNA Ladder for Safe Stains is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. (neb.com)
  • The 1 kb Plus DNA Ladder for Safe Stains is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. (neb.com)
  • The 1 kb Plus DNA Ladder for Safe Stains might be used on SYBR Green I precast agarose gels but might require further dilution and optimization. (neb.com)
  • The 1 kb Plus DNA Ladder for Safe Stains is not intended for use on short mini-gels or fast electrophoresis systems. (neb.com)
  • Agarose gel electrophoresis was carried out on the qPCR products using 1.5% agarose gel with a standard DNA ladder (100 bp), visualized under UV transilluminator and the image taken using digital camera. (scialert.net)
  • I used the 1 Kb Plus DNA Ladder (Invitrogen) here. (bitesizebio.com)
  • The DNA Ladder is suitable for use as molecular weight standards for agarose gel electrophoresis. (transgenbiotech.com)
  • Stable incorporations of T-DNA into the citrus genome have been confirmed with both histochemical staining of GUS activity and molecular analyses. (springer.com)
  • Once the DNA has been extracted, it can then be amplified using the GenomePlex® Whole Genome Amplification protocol. (sigmaaldrich.com)
  • The replication of HBV involves the production of covalently closed circular DNA (cccDNA) from the HBV genome through the repair of virion relaxed circular DNA (rcDNA) in the virion. (biomedcentral.com)
  • Tau-typing: a Nextflow pipeline for finding the best phylogenetic markers in the genome for molecular typing of microbial species. (cdc.gov)
  • Different Mycobacterium tuberculosis strains can be differentiated by genotyping methods that use information about genetic markers and their distribution in the genome ( 4 ). (cdc.gov)
  • Eukaryotic DNA polymerase β (pol β), the polymerase thought to be responsible for DNA repair synthesis, has been extensively characterized in rats and humans. (biomedcentral.com)
  • The former domain possesses a template-binding function and a dRPase activity to remove the 5'-deoxyribose phosphate, the latter domain possesses DNA polymerase activity. (biomedcentral.com)
  • DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)
  • Characterization of sequence-related amplified polymorphism markers analysis of tree peony bud sports[J]. Scientia Horticulturae (Amsterdam), 2008, 115(3):0-267. (transgenbiotech.com)
  • Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis. (cdc.gov)
  • Nationwide, population-based observational study of the molecular epidemiology and temporal trend of carbapenemase-producing Enterobacterales in Norway, 2015 to 2021. (cdc.gov)
  • The S. cerevisiae ORC binds to specific DNA sequences throughout the cell cycle but becomes active only when it binds to the replication initiator Cdc6. (nature.com)
  • In the budding yeast Saccharomyces cerevisiae , there are over 400 origins of DNA replication located on 16 chromosomes and they can function as autonomously replicating sequences (ARSs) when inserted into a plasmid 19 . (nature.com)
  • Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)
  • Adeno-associated virus is a non-enveloped virus that can be engineered to efficiently deliver DNA sequences of interest into target cells with a low immunogenicity. (clinlabint.com)
  • and 1,325 basepairs and having different DNA sequences compared to that of slow growth shrimp. (kkp.go.id)
  • The slightly higher variability of the hsp60 gene sequences with respect to the 16S rRNA sequences offers better opportunities to design or develop molecular assays, allowing identification and differentiation of closely related species. (biomedcentral.com)
  • Amino acid sequences of the putative serine protease open up studying frames FN1426 (Fsp25586) (A), and FNV0835 (Fsp49256) (B). Crimson emphasize signifies sequences recognized by mass spectrometry of the ninety nine kDa serine protease of F. nucleatum ATCC 25586 (A), and of the fifty five kDa serine protease of F. nucleatum ATCC 49256 (B). doi:10.1371/journal.pone.0111329.g003 DNA was isolated from F. nucleatum ATCC 25586 as explained above. (caspase1inhibitor.com)
  • An efficient method for high molecular weight bacterial DNA extraction suitable for shotgun metagenomics from skin swabs. (cdc.gov)
  • Azithromycin for bacterial watery diarrhea: A reanalysis of the AntiBiotics for Children with severe Diarrhea (ABCD) trial incorporating molecular diagnostics. (cdc.gov)
  • In this investigation, we aimed to detect some biochemical and/or molecular markers related to resistant genes for FW disease in some genotypes of faba bean. (ppjonline.org)
  • Methods: We designed two new pairs of primers for a PCR method from two conserved genes, cysteine proteinase B (cpb) and N-acetylglucosamine-6-phosfate deacetylase-like protein (nagA), as specific markers for those two respective subgenera. (bvsalud.org)
  • Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. (neb.com)
  • A previously described Taenia saginata HDP2 DNA sequence, a 4-kb polymorphic fragment, was previously used as the basis for developing PCR diagnostic protocols for the species-specific discrimination of T. saginata from T. solium and for the differentiation of T. saginata from T. asiatica . (biomedcentral.com)
  • Lane 2 corresponds to a nondigested fragment, and lane 1 shows DNA molecular-weight markers. (jamanetwork.com)
  • The results illuminate the molecular mechanism of a critical biochemical step in the licensing of eukaryotic replication origins. (nature.com)
  • Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. (ppjonline.org)
  • The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. (ppjonline.org)
  • Effect of age, high-fat diet, and rat strain on serum biomarkers and telomere length and global DNA methylation in peripheral blood mononuclear cells. (cdc.gov)
  • A reduction in the number of cells, the change in structure and functioning of organs, permanent change in DNA methylation, and in gene expression have also been considered to be molecular mechanisms responsible for fetal programming 1 . (bvsalud.org)
  • this metabolite can modify DNA methylation of normal neural and glial progenitor cells causing them to produce neoplastic glioma cells. (msdmanuals.com)
  • Methylation-based markers of aging and lifestyle-related factors and risk of breast cancer: a pooled analysis of four prospective studies. (who.int)
  • With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. (springer.com)
  • Kubisch CWicklein EMJentsch TJ Molecular diagnosis of McArdle's disease: revised genomic structure of the myophosphorylase gene and identification of a novel mutation. (jamanetwork.com)
  • Their binding to DNA inhibits synthesis of nucleic acids. (lookformedical.com)
  • A cytotoxic polypeptide quinoxaline antibiotic isolated from Streptomyces echinatus that binds to DNA and inhibits RNA synthesis. (lookformedical.com)
  • Other SNPs are related to low-molecular-weight metabolite synthesis, and may be associated with flavonoid biosynthesis and circadian rhythm-related metabolic processes. (peerj.com)
  • 2000). The nucleotide excision repair (NER), recombinational repair (Rec), and spore photoproduct (SP) lyase DNA repair pathways are major determinants of spore resistance to 254-nm UV-C radiation in the laboratory ( Fig. 1 ). (photobiology.com)
  • DNA and protein standards are available commercially in a wide range of sizes, and are often provided with pre-stained or color-coded bands for easy identification. (wikipedia.org)
  • The ORC-Cdc6 complex (product 1) assembles in step 1 around origin DNA and with the help of another replication initiator protein, Cdt1, it recruits the Mcm2-7 hexamer to the origin in step 2. (nature.com)
  • A model is proposed where the relative levels of active antagonist (RsbV1) and switch-protein anti-sigma factor (RsbW) control the availability of σ 66 and subsequently act as a molecular 'throttle' for Chlamydia growth and development. (plos.org)
  • When I began a master's program in 2008, the lab task I hated more than anything was running agarose gels for DNA. (bitesizebio.com)
  • Elevated excitability and its ac- and the molecular mechanisms of memory. (lu.se)
  • Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
  • Tsujino SShanske SDiMauro S Molecular heterogeneity of myophosphorylase deficiency (McArdle's disease). (jamanetwork.com)
  • Our characterization of pol β from a model fish organism contributes to the study of the function and evolution of DNA polymerases, which are emerging as important cellular targets for chemical intervention in the development of anticancer agents. (biomedcentral.com)
  • Molecular characterization of myophosphorylase deficiency in a group of patients from northern Italy. (jamanetwork.com)
  • Faster, Even Cooler DNA Gels! (bitesizebio.com)
  • Even though we added the DNA stain directly to the molten agarose and didn't have to spend time staining gels, gel electrophoresis still ate up entire days in the lab. (bitesizebio.com)
  • I was introduced to Bitesize Bio for the first time when I came across this article, "Faster, Cooler DNA gels" . (bitesizebio.com)
  • Ready-to-use molecular weight marker, designed for fast size determination of nucleic acids from 100 bp to 2000 bp. (gcbiotech.com)
  • Ready-to-use molecular weight marker with higher intensity bands for easy orientation, used for size determination of nucleic acids from 200 bp to 10 kb. (gcbiotech.com)
  • citation needed] Generally speaking, Orange G migrates faster than bromophenol blue, which migrates faster than xylene cyanol, but the apparent "sizes" of these dyes (compared to DNA molecules) varies with the concentration of agarose and the buffer system used. (wikipedia.org)
  • Here strand switching occurs by the second round of PCR and larger single stranded DNA molecules are built up extending from the outer primers until they overlap. (biomedcentral.com)
  • The agarose gel, as a molecular sieve, has network structure, and the material molecules will be resisted when passing through. (yeasenbiotech.com)
  • The DNA molecules of different sizes are charged differently, and the force in the electric field is also different, so the speed of migration from the negative electrode to the positive electrode is unequal. (yeasenbiotech.com)
  • It is mainly used for DNA cutting gel recovery, DNA separation, and proving whether the DNA is recombined and whether the plasmid is cut successfully. (yeasenbiotech.com)
  • exposures and lifestyle alter critical advances as well as the uniqueness and These developments have also led to molecular pathways and promote cancer strengths of IARC. (who.int)
  • Microorganism detection methods that detect microbial DNA rather than the microorganisms themselves have been introduced in both research and clinical laboratories. (bvsalud.org)
  • The study aimed to detect the molecular prevalence of urine schistosomiasis and evaluate microscopic examination vs. PCR technique for detection of Schistosoma haematobium ( S. haematobium ) in urine of patients with suggestive symptoms or previous history of urine schistosomiasis coming from endemic regions. (springeropen.com)
  • The use of different polymerases, DNA/primer ratios or different annealing temperatures may lead to a discrepancy in the results obtained in different laboratories[ 24 ]. (biomedcentral.com)
  • The low sensitivity of these conventional tests makes molecular detection the diagnostic method of choice. (springeropen.com)
  • The current study aimed to assess the true (molecular) prevalence of S. haematobium in Sohag University Hospital, Governorate of Sohag, using the PCR assay to detect DNA of S. haematobium in urine vs. urine microscopy and to evaluate the diagnostic performance of microscopy and also to determine the associated variable that can be a predicator for the occurrence of urinary schistosomiasis. (springeropen.com)
  • Molecular methods have been used to detect microorganisms that are impossible or difficult to culture. (bvsalud.org)
  • Molecular tests were used to detect pathogens. (who.int)
  • The next tier of studies will examine the contribution of an occupational exposure (e.g., welding fume inhalation) in combination with diet, age, and strain, to assess changes in the molecular responses of isolated PBMCs. (cdc.gov)
  • Therefore, these markers can be used in the early selection of resistant genotypes to provide the effort and cost resulting from the use of traditional breeding methods. (ppjonline.org)
  • Accuracy of high-risk HPV DNA PCR, p16((INK4a)) immunohistochemistry or the combination of both to diagnose HPV-driven oropharyngeal cancer. (who.int)
  • For instance, in a 1% agarose gel made in TAE buffer (Tris-acetate-EDTA), xylene cyanol migrates at the speed of a 3000 base pair (bp) molecule of DNA and bromophenol blue migrates at 400 bp. (wikipedia.org)
  • Time course of CLP-induced DNA fragmentation in thymocytes. (jci.org)
  • High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more. (parstous.com)
  • As you can see in this picture of a 1% gel made in LAB + GelRed, even the high molecular weight bands are pretty well resolved. (bitesizebio.com)
  • 2004) Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media . (bitesizebio.com)
  • The high reproducible quality of the pHelper-Kan plasmid DNA is maintained and verified by the company's standardized manufacturing technology. (clinlabint.com)
  • High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11. (jamanetwork.com)
  • Additional, high molecular weight PCR products were amplified by some of the primer pairs, but they did not interfere with the scoring of alleles. (psu.edu)
  • These species share a high sequence homology of 16S rDNA and several molecular techniques already applied to discriminate among them give ambiguous results. (biomedcentral.com)
  • The Origin Recognition Complex (ORC) binds to sites in chromosomes to specify the location of origins of DNA replication. (nature.com)
  • DNA cleavage is inhibited where the ligand binds to DNA. (lookformedical.com)
  • Manufactured in dedicated clean-rooms with mandated changeovers before new production cycles - AMSBIO introduces new pHelper-Kan plasmid DNA optimized for Adeno-Associated Virus (AAV) manufacturing. (clinlabint.com)
  • Photograph of electrophoretic gel for determining the specificity of a PCR assay for detecting DNA of M suis in human and swine blood samples, with DNA from other microorganisms used for reference. (avma.org)
  • However, the molecular changes driving lung cancer invasion and metastasis remain unclear. (aacrjournals.org)
  • It has been unclear at the molecular level how Cdc6 activates ORC, converting it to an active recruiter of the Mcm2-7 hexamer, the core of the replicative helicase. (nature.com)
  • Some markers generate a series of colored bands with known mobilities, while others produce a single band of a specific color that can be used as a reference point. (wikipedia.org)
  • The structure reveals that Cdc6 contributes to origin DNA recognition via its winged helix domain (WHD) and its initiator-specific motif. (nature.com)
  • ii) Spore DNA damage correction during spore germination is dependent upon nucleotide excision repair, recombination repair, and the SP-specific SP lyase repair systems. (photobiology.com)
  • The objective of the current study was to determine if age, diet, and genetic disposition (animal strain) in an animal model had early effects on specific molecular markers in circulating peripheral blood mononuclear cells (PBMCs). (cdc.gov)
  • The Section comprises two identify specific molecular changes low- and middle-income countries. (who.int)
  • This work illustrates a simple and cheap molecular tool for the identification of Bifidobacterium species. (biomedcentral.com)
  • In recent years alternative molecular markers have been proposed for bifidobacteria identification (e.g. hsp60 , recA , tuf , atpD , dnaK ) and Ventura et al. (biomedcentral.com)
  • Tracking dyes are added to the electrophoresis buffer rather to provide a visual marker of the buffer front. (wikipedia.org)
  • By using sodium borate (SB) electrophoresis buffer, you can crank up the voltage on the gel box without worrying about distorted DNA bands or even that the gel will melt. (bitesizebio.com)
  • Always use the same batch of buffer to prepare the agarose as to run the gel since small differences in ionic strength can affect migration of DNA. (qiagen.com)
  • I may even have used Google Scholar this time - that's how serious I was about finding a single buffer that could handle the range of DNA sizes I worked with. (bitesizebio.com)
  • DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). (lookformedical.com)