• Gram-negative spectrum notable for activity against Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, Proteus mirabilis, Proteus vulgaris . (mhmedical.com)
  • In tests, these variants were effective against some of the most dangerous hospital-acquired bacterial infections including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhimurium, including those demonstrating resistance to existing antibiotics. (growkudos.com)
  • We adapted a broth microdilution method for determining chlorhexidine MICs, poured panels, established quality control ranges, and tested Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae complex isolates collected at three U.S. sites. (cdc.gov)
  • In this study, we molecularly characterized 12 NDM-1 producing clinical Enterobacteriaceae ( Klebsiella pneumoniae , Escherichia coli , Enterobacter cloacae ) isolates that were part of a collection of non-carbapenem susceptible isolates obtained during a one-year period. (who.int)
  • The isolates appear clonally unrelated with MLST, revealing a diversity of clonal types among the Klebsiella pneumoniae and Escherichia coli isolates. (who.int)
  • The first carbapenem-resistant NDM-1 isolates characterized in 2009 were Klebsiella pneumoniae and Escherichia coli isolated from a Swedish patient who had sought medical care in New Delhi, India. (who.int)
  • The 52 isolates comprised the following species: 31 Klebsiella pneumoniae , 13 Escherichia coli , seven Enterobacter cloacae and one Enterobacter aerogenes . (who.int)
  • The was located on IncU-type plasmids in both isolates, which QnrA determinants have been reported worldwide in many resulted in increased MIC values of quinolones and fl uo- roquinolones, once they were transferred into Escherichia enterobacterial species, and 6 of them are known so far coli . (cdc.gov)
  • In this study, we investigated the presence of plasmid-mediated quinolone resistance (PMQR) genes among 101 ciprofloxacin-resistant urinary Escherichia coli isolates and searched for mutations in the quinolone-resistance-determining regions (QRDRs) of the DNA gyrase and topoisomerase IV genes in PMQR-carrying isolates. (scielo.br)
  • The aims of this research work were to determine the patterns of antibiotic resistance in Escherichia coli isolates from the meat of wild or domestically reared pigeons from Spain, to detect the presence of virulence and antibiotic resistance genes, and to carry out a phylogenetic classification of the isolates. (mdpi.com)
  • On the basis of the sequence information, we performed DNA amplification for sequencing and single-strand conformation polymorphism analysis to examine the presumed quinolone resistance regions of gyrA and gyrB from reference strains (n = 4) and clinical isolates (n = 55). (omicsdi.org)
  • The gyrA and gyrB sequence information will facilitate analysis of the mechanisms of resistance to drugs which target the gyrase and the implementation of rapid strategies for the estimation of FQ susceptibility in clinical M. tuberculosis isolates. (omicsdi.org)
  • Cloning and nucleotide sequence of the DNA gyrase gyrA gene from Serratia marcescens and characterization of mutations in gyrA of quinolone-resistant clinical isolates. (omicsdi.org)
  • DNA gyrase mutations are a major cause of quinolone resistance in Mycobacterium tuberculosis We therefore conducted the first comprehensive study to determine the diversity of gyrase mutations in pre-extensively drug-resistant (pre-XDR) (n = 71) and extensively drug-resistant (XDR) (n = 30) Thai clinical tuberculosis (TB) isolates. (omicsdi.org)
  • All NTM isolates, representing 183 rapidly growing and 131 slowly growing organisms, were previously identified by Sanger DNA sequencing of the full-length 16S rRNA gene, and region V of the rpoB gene. (cdc.gov)
  • The site of action is bacterial gyrase, a synthesis promoting enzyme. (drugs.com)
  • Inhibits bacterial DNA synthesis by inhibiting DNA gyrase enzyme. (mhmedical.com)
  • It inhibits DNA gyrase, a bacterial enzyme causing DNA supercoiling and, subsequently, stability of bacterial DNA (as DNA chain destabilization results in cell death). (pharmasyntez.com)
  • Compounds that revealed the best activity were subjected to further biological studies to determine their minimum inhibitory concentrations (MICs) against the selected pathogens as well as their in vitro activity against the E. coli DNA gyrase, to realize whether their antimicrobial action is mediated via inhibition of this enzyme. (olmdiagnostics.com)
  • A subsequent in vitro investigation against the bacterial DNA gyrase target enzyme revealed a significant potent inhibitory activity of quinoline derivative 14, which can be observed from its IC50 value (3.39 μM). (olmdiagnostics.com)
  • Also, a molecular docking study of the most active compounds was carried out to explore the binding affinity of the new ligands toward the active site of DNA gyrase enzyme as a proposed target of their activity. (olmdiagnostics.com)
  • This enzyme binds to DNA and, through a series of elegant movements, twists it up in a process known as supercoiling, vital for cells to function properly. (growkudos.com)
  • 4] Failure of DNA to properly separate during these processes results in a bacterium not being able to divide normally or produce functional proteins. (icr.org)
  • We demonstrated that Escherichia coli cells exposed to levofloxacin (LVFX), a fluoroquinolone (FQ), induce the syntheses of heat shock proteins and RecA. (biomedcentral.com)
  • We analyzed the proteins induced in Escherichia coli by exposure to FQs, then examined the susceptibilities to these agents of E. coli strains with mutations in the genes encoding these proteins. (biomedcentral.com)
  • These proteins were expressed in Escherichia coli, and the combination of GyrA and GyrB exhibited ATP-dependent supercoiling activity. (omicsdi.org)
  • Quinolone carboxylic acid derivatives are classified as DNA gyrase inhibitors. (drugs.com)
  • The predicted binding mode explains the competitive inhibitory mechanism with respect to ATP and forms a useful basis for further development of potent DNA gyrase inhibitors. (hal.science)
  • Herein, we report the synthesis and in vitro antimicrobial evaluation of novel quinoline derivatives as DNA gyrase inhibitors. (olmdiagnostics.com)
  • Fluoroquinolones inhibit bacterial DNA synthesis and, consequently, growth by inhibiting DNA gyrase and topoisomerases, which are required for replication, transcription, and translation of genetic material. (medscape.com)
  • Quinolone resistance among Enterobacteriaceae originally occurred due to chromosomal mutations in the quinolone-resistance-determining regions (QRDRs) of the gyrA and gyrB genes, which encode the gyrase A and B subunits, respectively and the parC and parE genes, which encode topoisomerase IV subunits (Hernández et al. (scielo.br)
  • This demonstrates that the primary structure of gyrase determines intrinsic quinolone resistance and was supported by a three-dimensional model of N-terminal GyrA. (omicsdi.org)
  • To analyze the role of DNA gyrase in quinolone resistance of B. fragilis, we isolated mutant strains by stepwise selection for resistance to increasing concentrations of levofloxacin. (omicsdi.org)
  • Its main function is to inhibit the deoxyribonucleic acid (DNA) gyrase of bacteria, so that the synthesis of deoxyribonucleic acid (DNA) protein is disturbed, so that the cells can no longer divide, thereby playing a sterilizing role. (ballyabio.com)
  • We have hope that the unusual nature of the interaction between albicidin and gyrase will allow us and others to use it as a base to develop antibiotics that can overcome the threat of antibiotic resistant bacteria" commented Elizabeth Michalczyk, a PhD student in the Heddle lab and one of the lead authors of the work. (growkudos.com)
  • Escherichia coli rapidly develops resistance, particularly that associated with multiple drug resistance (MDR) when exposed to selected antimicrobials. (vin.com)
  • Antimicrobial resistant strains of Escherichia coli ( E. coli ) are a common cause of UTI which were identified as a treatment challenge. (biomedcentral.com)
  • Total genomic DNA from 73 strains was extracted by conventional methods. (upenn.edu)
  • The amplified DNA products were separated electrophoretically in a 1% agarose gel containing ethidium bromide and the banding patterns were compared among different strains. (upenn.edu)
  • The addition of the Southern blot analysis to the AP-PCR analysis gave rise to a total of 30 DNA profiles among the 73 A. actinomycetemcomitans study strains. (upenn.edu)
  • Streptomyces-produced quinolone and coumarin antibiotics, such as novobiocin, interfere with a protein called gyrase that assists in the normal separation of double-stranded DNA during replication of DNA or transcription of messenger RNA. (icr.org)
  • 大腸菌 / DNA複製 / DNA replication / DNAポリメラーゼ / ATP / DnaA / Escherichia coli / DnaA protein / 細胞分裂 / Fプラスミド / 細胞周期 / ゲノム自己増殖の終結 / ゲノム自己増殖の開 (nii.ac.jp)
  • Therefore, molecular docking was also used in the present study to simulate the binding of cordycepin with different antibacterial targets, such as β-ketoacyl-(acyl-carrier-protein) synthase III (FabH), DNA gyrase B and thymidylate kinase, in order to identify the most likely antimicrobial mechanism of cordycepin. (spandidos-publications.com)
  • By a structure-based approach we have previously discovered compound 1, an indolinone derivative, possessing inhibitory activity against DNA gyrase. (hal.science)
  • To address how gyrase copes with these topological challenges, we used high-speed single-molecule fluorescence imaging in live Escherichia coli cells. (york.ac.uk)
  • Bacterial DNA gyrase introduces negative supercoils into chromosomal DNA and relaxes positive supercoils introduced by replication and transiently by transcription. (york.ac.uk)
  • An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. (omicsdi.org)
  • Using mass spectrometry, NMR spectroscopy, and fluorescence experiments we have demonstrated that compound 1 binds reversibly to the ATP-binding site of the 24 kDa N-terminal fragment of DNA gyrase B from Escherichia coli (GyrB24) with low micromolar affinity. (hal.science)
  • The membrane was probed with a previously characterized 5.2 kilobases (kb) DNA fragment cloned from A. actinomycetemcomitans strain Y4. (upenn.edu)
  • Biophysical characterization of an indolinone inhibitor in the ATP-binding site of DNA gyrase. (hal.science)
  • Unlike replication of many Escherichia coli plasmids, replication of cyanobacterial plasmids did not require added ATP, was not inhibited by omission of the ribonucleotides, and was insensitive to the RNA polymerase inhibitor rifampicin and the gyrase inhibitor novobiocin, but was inhibited by ethidium bromide. (upenn.edu)
  • Gyrase catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes (PubMed:22457352). (wuxibiortus.com)
  • The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined. (omicsdi.org)
  • Results of the DNA-shift assays and reporter gene expression analysis are consistent with the idea that SimReg1 activates transcription of simocyclinone biosynthesis, transporter genes, regulatory gene simReg3 and his own transcription. (springeropen.com)
  • Herein, we describe the generation and analysis of the mutant strain deficient in the simReg1 gene, mobility shift DNA-binding assays of His-SimReg1 to putative promoter regions and propose a putative model for regulation of the biosynthesis and export of simocyclinones. (springeropen.com)
  • Escherichia coli is a common cause of community-acquired urinary tract infections (CA-UTIs). (scielo.br)
  • Escherichia coli , a member of the family Enterobacteriaceae, is a lactose fermenter, causing a distinct color on diagnostic agar. (vin.com)
  • at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli (PubMed:22457352). (wuxibiortus.com)
  • Removal of these positive supercoils is essential for replication fork progression and for the overall unlinking of the two duplex DNA strands, as well as for ongoing transcription. (york.ac.uk)
  • Despite its known antibiotic potential and low toxicity in pre-clinical experiments, pharmaceutical development of albicidin has been hampered by the fact that scientists did not know precisely how it interacted with DNA gyrase. (growkudos.com)
  • Selected flavonoids were tested for their ability to inhibit the catalytic activity of DNA topoisomerase (topo) I and II. (ucy.ac.cy)
  • The results of computational molecular docking analysis indicated that the interaction energy between cordycepin and NAD+‑dependent DNA ligase (LigA) was lower than that between cordycepin and other common antibacterial targets. (spandidos-publications.com)
  • We demonstrate that at least 300 gyrase molecules are stably bound to the chromosome at any time, with ~12 enzymes enriched near each replication fork. (york.ac.uk)
  • The replacement of M74 in GyrA, A83 in GyrA, and R447 in GyrB of Mycobacterium tuberculosis gyrase by their Escherichia coli homologs resulted in active enzymes as quinolone susceptible as the E. coli gyrase. (omicsdi.org)
  • Relaxes negatively supercoiled DNA in an ATP-independent manner (PubMed:337300). (wuxibiortus.com)