• Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood. (asm.org)
  • In clinical evaluations, pathogens of more than 200 bacterial and 65 fungal genera have been identified so far. (bionity.com)
  • Here we report the development of DNA microarray for the identification of pathogens causing bloodstream infections. (elsevier.com)
  • This DNA microarray-based pathogen diagnosis takes approximately 10 h starting from a positive blood culture, considerably reducing time required to sufficiently identify pathogens by subsequent agar-culture and biochemical tests which requires altogether at least 1-3 days. (elsevier.com)
  • Thus, the DNA microarray reported here should be useful for the effective identification of microbial pathogens in blood cultures from septicemic patients. (elsevier.com)
  • Thermally dimorphic fungal pathogens, including Histoplasma capsulatum, are a class of soil fungi that undergo a dramatic change in cell shape and virulence gene expression in response to host temperature. (omictools.com)
  • As there are a lot of antibacterial and anti-fungal resistant pathogens, researchers attempt to substitute antimicrobial drugs with various medical plants and novel nanoparticles . (bvsalud.org)
  • In rice ( Oryza sativa ), the chitin elicitor binding protein (CEBiP) recognizes chitin oligosaccharides released from the cell walls of fungal pathogens. (plantcell.org)
  • The whole of creation was then subjected to a negative turn of events, including the emergence of fungal pathogens that cause death and disease in plants and animals. (icr.org)
  • Recently, several new studies were published showing how fungal pathogens adapted to their hosts through large-scale genomic changes involving a type of genome degradation marked by gene losses. (icr.org)
  • My current research studies include molecular diagnostics (root pathogens, foliar pathogens, fungal endophytes, fungal decomposers) and evolutionary relationships among forest hosts and pathogens. (fed.us)
  • Precise DNA-based identification of forest pathogens and beneficial microbes is essential to determine their distribution, ecological role, and predictions of future behavior (including invasiveness), so that appropriate management practices can be developed and/or applied. (fed.us)
  • This is the first time that we've used genetic barcodes-DNA sequences unique to each living organism-to characterize an entire amphibian community," said Eldredge Bermingham, STRI director and co-author. (innovations-report.com)
  • Dust was vacuumed from 32 classrooms and analysed for different endotoxin as 3-hydroxy fatty acids (3-OH), muramic acid, ergosterol, allergens and five fungal DNA sequences. (ersjournals.com)
  • Five sequences of fungal DNA were analyzed by quantitative PCR. (diva-portal.org)
  • Promoters play a central role in transcription initiation and gene regulation, so it is necessary for these DNA regions to be differentiated from non-promoter sequences. (jbsdonline.com)
  • We have devised a new strategy to clone DNA sequences from an yeast autonomously-propagating plasmid into a non-autonomous integrative vector by in-vivo recombination. (nih.gov)
  • Updated DNA Sequences ===================== AC007505 AC007505 134499bp DNA PLN 09-OCT-1999 Arabidopsis thaliana chromosome I BAC F28L22 genomic sequence, complete sequence. (bio.net)
  • Coding DNA represents only a small fraction of eukaryotic genomic DNA: the bulk of the DNA is noncoding, much of which is made up of repetitive sequences. (qiagen.com)
  • GenBank from the NCBI is an amazing and invaluable resource for DNA sequences, and combined with the searching tool BLAST it is really easy to identify your unknown gene sequence. (rhizobia.co.nz)
  • The main aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for organismal identification and taxonomic clarification. (slideshare.net)
  • The N-terminal region of a number of fungal transcriptional regulatory proteins contains a Cys-rich motif that is involved in zinc-dependent binding of DNA. (ebi.ac.uk)
  • Viral DNA-binding proteins have served as good models to study the biochemistry of transcription regulation and chromatin dynamics. (biomedcentral.com)
  • Computational analysis of viral DNA-binding regulatory proteins and identification of their previously undetected homologs encoded by cellular genomes might lead to a better understanding of their function and evolution in both viral and cellular systems. (biomedcentral.com)
  • The phyletic range and the conserved DNA-binding domains of the viral regulatory proteins of the poxvirus D6R/N1R and baculoviral Bro protein families have not been previously defined. (biomedcentral.com)
  • Using computational analysis, we show that the amino-terminal module of the D6R/N1R proteins defines a novel, conserved DNA-binding domain (the KilA-N domain) that is found in a wide range of proteins of large bacterial and eukaryotic DNA viruses. (biomedcentral.com)
  • The amino-terminal module of the Bro proteins is another, distinct DNA-binding domain (the Bro-N domain) that is present in proteins whose domain architectures parallel those of the KilA-N domain-containing proteins. (biomedcentral.com)
  • We define a large class of novel viral DNA-binding proteins and their cellular homologs and identify their domain architectures. (biomedcentral.com)
  • Much less is known of the domain architecture and evolutionary history of those viral DNA-binding regulatory proteins whose cellular homologs have not (yet) been identified. (biomedcentral.com)
  • Proteins obviously play critical roles in diverse biological processes through interactions with other proteins, DNA, RNA, and small molecules both inside and outside cells. (genetics.org)
  • We dissect the circuit driven by three regulators that control yeast-phase growth, and demonstrate that these factors, including two deeply conserved Velvet family proteins of unknown function, associate with DNA. (omictools.com)
  • Once purified, plasmid DNA can be used in a wide variety of downstream applications such as sequencing, PCR, expression of proteins, transfection, and gene therapy. (qiagen.com)
  • Cytochrome P450 proteins (CYPs) play diverse and pivotal roles in fungal metabolism and adaptation to specific ecological niches. (biomedcentral.com)
  • Using pyrosequencing of the 16S rRNA gene and ITS2 region amplicons, we analysed the bacterial and fungal gut communities of the honey bee as affected by the host social status. (nature.com)
  • Luhung I, Wu Y, Xu S, Yamamoto N, Wei-Chung Chang V, Nazaroff WW (2018) Exploring temporal patterns of bacterial and fungal DNA accumulation on a ventilation system filter for a Singapore university library . (microbe.net)
  • Genomic DNA isolated from fungi is appropriate for PCR* and other molecular biology applications. (atcc.org)
  • There are other advantages to anther smut as well: the fungal models are safe for researchers to use, the fungus is easy to grow and, unlike humans, fungi have small genomes that make the sequencing process simple. (amherst.edu)
  • The dimorphic probe hybridized with DNA from H . capsulatum , B . dermatitidis , C . immitis , P . brasiliensis , and P . marneffei but not with DNA from nondimorphic fungi. (asm.org)
  • In order to speed up and standardize polymerase chain reaction (PCR)-based detection of medically important fungi in clinical samples we established a combination of commercially available kits for DNA extraction, PCR amplification and detection of the amplicons. (nih.gov)
  • DNA was extracted from 100 mg samples from each of the following fungi: lane 1 from Ganoderma lucidum , lane 2 from Cordyceps sinensis , lane 3 from Agaricus campestris , and lane 4 from Russula foetens . (omegabiotek.com)
  • Purified genomic DNA from various fungi was isolated with the E.Z.N.A.® HP Fungal DNA Kit. (omegabiotek.com)
  • In general, increased exposure to f-DNA was positively related to higher prevalence of all symptoms/diseases (in a dose dependent manner), and significantly related to a decrement of pulmonary function independent from type of fungi. (aspergillus.org.uk)
  • Here we present a global survey of fungi from indoor environments ( n = 72), using both taxonomic and phylogeny-informative molecular markers to determine whether global or local indoor factors determine indoor fungal composition. (pnas.org)
  • They are present in plants, bacteria, various vertebrates, and fungi and were originally considered as sensory photoreceptors because of their incapability to repair cyclobutane pyrimidine dimer (CPD) lesions in duplex DNA. (openaire.eu)
  • Introduction The utilization of fungal biodegradation involves the controlled usage of these specially cultivated fungi to treat contaminants. (environmental-expert.com)
  • The first step in performing molecular diagnostics on wood-decay fungi is to obtain analyzable fungal DNA. (environmental-expert.com)
  • To prevent the spread of fungi and its harmful effects, try using an anti-fungal spray like MesoSilver. (ehow.com)
  • Fungal genetics uses yeast , and filamentous fungi as model organisms for eukaryotic genetic research, including cell cycle regulation, chromatin structure and gene regulation . (wikipedia.org)
  • Sequencing of a high number of fungal genomes has become possible due to the development of next generation sequencing techniques (NGS). (springer.com)
  • It's a difficult question to explore since the answer involves tracking human DNA and the harmful mutations genomes accumulate over long periods of time. (amherst.edu)
  • A detailed analysis of the KilA-N and Bro-N domains and the associated domains points to extensive domain shuffling and lineage-specific gene family expansion within DNA virus genomes. (biomedcentral.com)
  • The comparative analysis of these genomes relative to one another and to other sequenced fungal genomes is provided here. (plos.org)
  • The genomes of almost all organisms are DNA, the only exceptions being some viruses that have RNA genomes. (qiagen.com)
  • Viral DNA genomes are relatively small and can be single- or double-stranded, linear, or circular. (qiagen.com)
  • All other organisms have double-stranded DNA genomes. (qiagen.com)
  • Fungal genomes encode extremely variable "CYPomes" ranging from one to more than 300 CYPs. (biomedcentral.com)
  • Despite the rapid growth of sequenced fungal and oomycete genomes and the resulting influx of predicted CYPs, the vast majority of CYPs remain functionally uncharacterized. (biomedcentral.com)
  • These features will facilitate functional annotation and classification of CYPs encoded by newly sequenced fungal and oomycete genomes. (biomedcentral.com)
  • Hierarchical clustering analysis (HCA) allowed us to define five major clusters, significantly associated with fungal load ( p = 0.029), in which copy number of mitochondrial genes was significantly different among them. (frontiersin.org)
  • The recent utilization of molecular methods such as internal transcribed spacer (ITS) sequencing of ribosomal RNA genes has provided improved insight into the diversity of fungal bioaerosols in indoor, outdoor and occupational environments. (cdc.gov)
  • In the filamentous fungus Neurospora crassa , genes unpaired during meiosis are silenced by a process known as meiotic silencing by unpaired DNA (MSUD). (g3journal.org)
  • Genomic DNA contains genes, discrete regions that encode a protein or RNA. (qiagen.com)
  • The uptake of donor DNA and its recombinational incorporation into the recipient chromosome depends on the expression of numerous bacterial genes whose products direct this process. (wikipedia.org)
  • These data demonstrate that the variation in the occurrence of the 1.2 Mb minichromosome did not arise by rearrangement of the genome of a progenitor strain but involved either large scale deletion or addition of DNA. (nih.gov)
  • It is being said that the modern human genome sequence may carry a leftover contribution from Neanderthal DNA. (i4u.com)
  • A gene library was prepared specifically from the 1.2 Mb minichromosome and ten independent DNA clones unique to this chromosome were identified by differential hybridisation to whole chromosome probes. (nih.gov)
  • 100 years ago (set as the control) to explore the impact of wildfire on the soil chemistry, soil microbial stoichiometry, and the fungal-to-bacterial gene ratio (F:B ratio). (springer.com)
  • Wildfire-induced reduction in vegetation biomass positively affected the fungal, but not the bacterial, gene copy number. (springer.com)
  • The method consists of a first step in which the replicative plasmid carrying the DNA fragment of interest forms a co-integrate with the non-replicative plasmid by an induced in-vivo reciprocal exchange accompanied by gene conversion. (nih.gov)
  • It also highlights the utility of gene sequencing in a postmortem setting for speciation of fungal organisms, both for providing a definitive diagnosis and additional information for infection control. (cap.org)
  • DNA methyltransferase, motifs IX-X. AF139372 AF139372 2115bp DNA PLN 10-OCT-1999 Arabidopsis thaliana DNA methyltransferase (METI) gene, partial cds. (bio.net)
  • A gene comprises the coding DNA sequence, as well as the associated regulatory elements that control gene expression. (qiagen.com)
  • Although much of the extraordinary diversity of the pan-fungal CYPome can be attributed to gene duplication and adaptive divergence, our analysis also suggests a few potential horizontal gene transfer events. (biomedcentral.com)
  • he ITS gene region has been selected as the official gene for fungal barcoding in a recent publication of the Proceedings of the National Academy of Sciences. (rhizobia.co.nz)
  • Large DNA viruses of bacteria and eukaryotes have complex life cycles with several distinct phases that involve diverse virus-host interactions. (biomedcentral.com)
  • In conclusion, the combination of Euphorbia prostrata and Pelargonium graveolens extracts with nanostructures showed synergic effects on eliminating the bacteria via DNA destruction and others mechanisms. (bvsalud.org)
  • Specific probes for H . capsulatum , B . dermatitidis , C . immitis , P . brasiliensis , P . marneffei , S . schenckii , C . neoformans , and P . carinii hybridized with homologous but not heterologous DNA. (asm.org)
  • The KilA-N domain is suggested to be homologous to the fungal DNA-binding APSES domain. (biomedcentral.com)
  • Detection of transcripts homologous to DNA plasmids in isolates from six anastomosis groups of Rhizoctonia solani' Ann.Phytopathol.Soc.Jpn.62. (nii.ac.jp)
  • Conjugation requires stable and extended contact between a donor and a recipient strain, is DNase resistant, and the transferred DNA is incorporated into the recipient chromosome by homologous recombination . (wikipedia.org)
  • This latest study, led by Leeds University and published in this month's Molecular Biology and Evolution , shows that a substantial fraction of the mitochondrial DNA lines (inherited by female descendants) have been evolving within ISEA for a much longer period, some since modern humans arrived about 50,000 years ago. (phys.org)
  • By examining mitochondrial DNA from their descendants in Southeast Asia and the Pacific, we now have strong evidence to support the flooding theory and this is possibly why Southeast Asia has a richer store of flood myths, more than any other region in the world. (phys.org)
  • It's a question that has baffled scientists since they began understanding DNA, and is one that Michael E. Hood, associate professor of biology, believes he can help answer-using a violet fungus that infects a tiny carnation-like flower. (amherst.edu)
  • On the basis of phyletic pattern analysis we present evidence for a probable viral origin of the fungus-specific cell-cycle regulatory transcription factors containing the APSES DNA-binding domain. (biomedcentral.com)
  • After penetration of the host surface, the fungal penetration peg differentiates to form a thin filamentous primary hypha and the fungus grows without causing disease symptoms. (plantcell.org)
  • Expression of the linear DNA plasmid pRS64 in the plant pathogenic fungus Rhizoctonia solani' Mol.Gen.Genet.245. (nii.ac.jp)
  • The complete nucleotide sequence and characterizatior of the linear DNA plasmids pRS64 from the plant pathogenic fungus Rhizoctonia solani' Curr.Genet. (nii.ac.jp)
  • I am trying to extract Dna from fungus (Alternaria alternata). (protocol-online.org)
  • The host immune system needs to recognize the distinct morphological forms of A. fumigatus to control fungal growth and prevent tissue invasion, whereas the fungus requires nutrients and needs to adapt to the hostile environment by escaping immune recognition and counteracting host responses. (bireme.br)
  • Queen Mary University of London researchers discovered the genetic sequence of ash tree that can treat fungal disease, ash dieb. (i4u.com)
  • Chemidlin Prévost-Bouré N, Christen R, Dequiedt S, Mougel C, Lelièvre M, Jolivet C, Shahbazkia HR, Guillou L, Arrouays D, Ranjard L (2011) Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR. (springer.com)
  • Results The quantitative result showed that total and bacterial DNA accumulated monotonically, rising to 41 ng/cm2 for total DNA and to 2.8 ng/cm2 for bacterial DNA over the 21-week period. (microbe.net)
  • The dip indicated a possible artifact of this sampling approach for quantitative analysis as DNA may not be conserved on the filter over the months-long service period. (microbe.net)
  • Within this environment, some of the most diverse and potentially influential members of the biotic community are the fungal endophytes (FEs) ( 7 , 8 , 14 ⇓ - 16 ). (pnas.org)
  • Three commonly used DNA extraction methodologies (UltraClean Soil kit, High Pure PCR Template kit, and EluQuik/DNeasy kit) were compared in terms of sensitivity and susceptibility to PCR inhibitors in dust for three common fungal bioaerosols, Aspergillus versicolor, Rhizopus microsporus and Wallemia sebi. (cdc.gov)
  • Minor cross-reactivity was observed for the B . dermititidis probe used against C . immitis DNA and for the H . capsulatum probe used against Candida albicans DNA. (asm.org)
  • Five commercially available extraction kits and an in-house DNA extraction method for the release of DNA from Candida albicans and Aspergillus niger cells were assessed for sensitivity, purity, duration, and cost. (asm.org)
  • The present study was conducted to characterize antimicrobial activities of Euphorbia prostrata and Pelargonium graveolens extract alone and in combination with [email protected] & Mn Fe (OH)3-NPs on the DNA cleavage of E. coli and also Bacillus subtilis , Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus , Aspergillus oryzae , and Candida albicans . (bvsalud.org)
  • Therefore, histopathologic examination of tissue sections may be the most rapid or only way in which to diagnose invasive fungal disease. (asm.org)
  • The QIAmp Tissue kit was the commercially available assay that yielded the same sensitivity and purity of fungal DNA release as the in-house protocol but was the most expensive method. (asm.org)
  • The system combines the reversible nucleic acid-binding properties of Omega's HiBind® matrix with the speed and versatility of spin columns to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. (omegabiotek.com)
  • DNA was extracted from 100 mg fresh fungal tissue as follows: lane 1 from Ganoderma lucidum , lane 2 from Cardyceps sinesis , lane 3 from Agaricus campetris , lane 4 from Russula foetens . (omegabiotek.com)
  • W hy is the Y chromosome, which determines male development, slowly disappearing in human DNA? (amherst.edu)
  • These ten probes also hybridised only to restriction-digested DNA of race 3 and were thus both chromosome- and strain-specific for Type B C. gloeosporioides. (nih.gov)
  • All ten chromosome-specific DNA probes hybridised to a 2.0 Mb chromosome in all races of C. gloeosporioides causing Type A anthracnose on Stylosanthes spp. (nih.gov)
  • AC007767 AC007767 122498bp DNA HTG 11-OCT-1999 Arabidopsis thaliana chromosome 1 clone F5D14, WORKING DRAFT SEQUENCE, 3 ordered pieces. (bio.net)
  • AC009519 AC009519 113170bp DNA HTG 09-OCT-1999 Arabidopsis thaliana chromosome 1 clone F1N19, WORKING DRAFT SEQUENCE, 1 ordered pieces. (bio.net)
  • AC010164 AC010164 103443bp DNA PLN 09-OCT-1999 Arabidopsis thaliana chromosome I BAC F14M2 genomic sequence, complete sequence. (bio.net)
  • AC010852 AC010852 92088bp DNA HTG 09-OCT-1999 Arabidopsis thaliana chromosome I clone TAMU-T12P18, WORKING DRAFT SEQUENCE, 3 unordered pieces. (bio.net)
  • AC011661 AC011661 76731bp DNA HTG 09-OCT-1999 Arabidopsis thaliana chromosome 1 clone T23J18, WORKING DRAFT SEQUENCE, 10 unordered pieces. (bio.net)
  • AC011663 AC011663 110572bp DNA HTG 09-OCT-1999 Arabidopsis thaliana chromosome III clone IGF-F1C9, WORKING DRAFT SEQUENCE, 5 unordered pieces. (bio.net)
  • AC011664 AC011664 102719bp DNA HTG 09-OCT-1999 Arabidopsis thaliana chromosome I clone IGF-F5A18, WORKING DRAFT SEQUENCE, 6 unordered pieces. (bio.net)
  • ATAC009895 AC009895 82289bp DNA PLN 09-OCT-1999 Arabidopsis thaliana chromosome III BAC T21P5 genomic sequence, complete sequence. (bio.net)
  • Organization of heterologous DNA inserts on the mouse meiotic chromosome core. (biomedsearch.com)
  • Others have shown temporal fluctuations of fungal assemblages in human environments and modest correlations with human activity, but global-scale patterns have not been examined, despite the manifest significance of biogeography in other microbial systems. (pnas.org)
  • The existence of global-scale patterns in fungal composition, however, is unexamined, despite evidence of biogeographical patterning in other microbial systems. (pnas.org)
  • The microbial C:N ratios were independent of the shifts in the fungal-to-bacterial ratio when C:N exceeded 12. (springer.com)
  • We conclude that microbial stoichiometry does not always respond to changes in the fungal-to-bacterial ratio and that wildfire-induced permafrost thawing does not accelerate microbial respiration. (springer.com)
  • 2014 ). A higher fungal-to-bacterial (F:B) ratio may, therefore, be associated with a higher C:N ratio for the total microbial biomass. (springer.com)
  • Reactive oxygen molecules with free radicals, which are a byproduct of cellular metabolism, can also be very harmful to DNA. (wikipedia.org)
  • Aim: To assess how indoor moulds confirmed by fungal DNA can affect respiratory health and pulmonary function in school-children. (aspergillus.org.uk)
  • Remarkably, building function has no significant effect on indoor fungal composition, despite stark contrasts between architecture and materials of some buildings in close proximity. (pnas.org)
  • Recent efforts to describe the processes shaping indoor and urban fungal composition show temporal effects ( 13 - 15 ) and modest correlations with human activity ( 16 ). (pnas.org)
  • Finally, as a result of the combination of presumably high dispersal rates between indoor habitats and the highly selective indoor environment, we expected little influence of the outdoor environment on fungal composition. (pnas.org)
  • We test these hypotheses ( i ) by examining whether the global distribution patterns of individual taxa show range limitation, ( ii ) by examining whether indoor fungal composition correlates with dispersal limitation or outdoor environment, and ( iii ) by testing an alternative hypothesis that indoor environment determines fungal composition by comparing nearby buildings with different architecture and function. (pnas.org)
  • The accumulation rate of bacterial DNA correlated with indoor occupancy level. (microbe.net)
  • This situation is perpetuated by a paucity of cultivable fungal strains available for phylogenetic research linked to these data sets. (duhnnae.com)
  • By comparing DNA profiles from unknown organisms to the databases, researchers can identify biological specimens quickly, and construct genetic lineages. (innovations-report.com)
  • Advances in the field over the last two decades have led occupational health researchers to turn to molecular-based approaches for identifying fungal hazards. (cdc.gov)
  • The method is particularly useful for the cloning of large DNA fragments and can be used for plasmids from organisms other than yeasts. (nih.gov)
  • Contrary to common ecological patterns, we show that fungal diversity is significantly higher in temperate zones than in the tropics, with distance from the equator being the best predictor of phylogenetic community similarity. (pnas.org)
  • Mutagens can be physical, such as radiation from UV rays, X-rays or extreme heat, or chemical (molecules that misplace base pairs or disrupt the helical shape of DNA). (wikipedia.org)