Loading...
  • sequences
  • Dust was vacuumed from 32 classrooms and analysed for different endotoxin as 3-hydroxy fatty acids (3-OH), muramic acid, ergosterol, allergens and five fungal DNA sequences. (ersjournals.com)
  • Promoters play a central role in transcription initiation and gene regulation, so it is necessary for these DNA regions to be differentiated from non-promoter sequences. (jbsdonline.com)
  • Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome. (wikipedia.org)
  • R-DNA technology uses palindromic sequences and leads to the production of sticky and blunt ends. (wikipedia.org)
  • In addition, DNA sequences that do not occur anywhere in nature may be created by the chemical synthesis of DNA, and incorporated into recombinant molecules. (wikipedia.org)
  • citation needed] Recombinant DNA differs from genetic recombination in that the former results from artificial methods in the test tube, while the latter is a normal biological process that results in the remixing of existing DNA sequences in essentially all organisms. (wikipedia.org)
  • The other difference is that cloning involves cutting and pasting DNA sequences, while PCR amplifies by copying an existing sequence. (wikipedia.org)
  • That is, their appearance, behavior and metabolism are usually unchanged, and the only way to demonstrate the presence of recombinant sequences is to examine the DNA itself, typically using a polymerase chain reaction (PCR) test. (wikipedia.org)
  • Mitochondrial DNA sequences have been used for phylogenetic studies in the dermatophytes. (wikipedia.org)
  • fungi
  • DNA was extracted from 100 mg samples from each of the following fungi: lane 1 from Ganoderma lucidum , lane 2 from Cordyceps sinensis , lane 3 from Agaricus campestris , and lane 4 from Russula foetens . (omegabiotek.com)
  • Genomic DNA isolated from fungi is appropriate for PCR* and other molecular biology applications. (atcc.org)
  • As in all mushroom-producing fungi, the fruit bodies (sporocarps) are the reproductive structures that are produced from fungal mycelium when the appropriate environmental conditions of temperature, humidity and nutrient availability are met. (wikipedia.org)
  • The geographic distribution of many of the fungi is uncertain, primarily because the identification of the fungal partners has not always been easy, especially prior to the application of DNA-based identification methods. (wikipedia.org)
  • clones
  • In standard cloning protocols, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into the host organism, (6) Selection of organisms containing recombinant DNA, and (7) Screening for clones with desired DNA inserts and biological properties. (wikipedia.org)
  • The application of DNA sequencing to fungal isolates and clones from environmental PCR has uncovered diverse fungal communities in ericoid roots. (wikipedia.org)
  • polymerase chain re
  • This preparation of high molecular weight DNA is appropriate for the use in the polymerase chain reaction (PCR)* process and other molecular biology applications. (atcc.org)
  • It is one of two most widely used methods, along with polymerase chain reaction (PCR), used to direct the replication of any specific DNA sequence chosen by the experimentalist. (wikipedia.org)
  • eukaryotic
  • The nonlinear correlation for eukaryotes, although claim of its existence contrasts the previous view that no correlation exists for this group of organisms, reflects disproportionately fast increasing noncoding DNA in increasingly large eukaryotic genomes. (wikipedia.org)
  • genes
  • In eukaryotes, but not prokaryotes, variation in genome size is not proportional to the number of genes, an observation that was deemed wholly counterintuitive before the discovery of non-coding DNA and which became known as the C-value paradox as a result. (wikipedia.org)
  • 2010) investigated plant genes involved in communication with order Glomales fungal partners (DMI1, DMI3, IPD3). (wikipedia.org)
  • associations
  • There were positive associations between levels of Aspergillus versicolor DNA and daytime breathlessness, between C14 3-OH and respiratory infections and between ergosterol and doctors diagnosed asthma. (ersjournals.com)
  • relatively
  • Flucytosine must not be used as a sole agent in life-threatening fungal infections due to relatively weak antifungal effects and fast development of resistance, but rather in combination with amphotericin B and/or azole antifungals such as fluconazole or itraconazole. (wikipedia.org)
  • Vectors are generally derived from plasmids or viruses, and represent relatively small segments of DNA that contain necessary genetic signals for replication, as well as additional elements for convenience in inserting foreign DNA, identifying cells that contain recombinant DNA, and, where appropriate, expressing the foreign DNA. (wikipedia.org)
  • plant
  • Evidence suggests that coils only function for a period of a few weeks before the plant cell and fungal hyphae begin to degrade. (wikipedia.org)
  • name
  • Recombinant DNA is the general name for a piece of DNA that has been created by the combination of at least two strands. (wikipedia.org)
  • molecular
  • Molecular cloning is the laboratory process used to create recombinant DNA. (wikipedia.org)
  • One is that molecular cloning involves replication of the DNA within a living cell, while PCR replicates DNA in the test tube, free of living cells. (wikipedia.org)
  • The choice of vector for molecular cloning depends on the choice of host organism, the size of the DNA to be cloned, and whether and how the foreign DNA is to be expressed. (wikipedia.org)
  • analysis
  • SepsiTest-UMD CE IVD includes all reagents and consumables necessary for DNA extraction and broad range PCR or Real-Time PCR analysis as well as primers for Sanger-sequencing. (bionity.com)
  • He is considered one of today's leading researchers "in the analysis of fungal relationships through DNA analysis. (wikipedia.org)
  • variety
  • The DNA segments can be combined by using a variety of methods, such as restriction enzyme/ligase cloning or Gibson assembly. (wikipedia.org)
  • order
  • The majority of research with ericoid mycorrhizal fungal physiology and function has focused on fungal isolates morphologically identified as Rhizoscyphus ericae, in the Ascomycota order Helotiales. (wikipedia.org)
  • Properties
  • The system combines the reversible nucleic acid-binding properties of Omega's HiBind® matrix with the speed and versatility of spin columns to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. (omegabiotek.com)
  • host
  • When recombinant DNA encoding a protein is introduced into a host organism, the recombinant protein is not necessarily produced. (wikipedia.org)
  • Following transplantation into the host organism, the foreign DNA contained within the recombinant DNA construct may or may not be expressed. (wikipedia.org)