• The recombinant immunoblot assay is used to confirm HCV infection. (medscape.com)
  • RIBATM Recombinant immunoblot assay. (cdc.gov)
  • However, in clinical practice, the most common method for diagnosing established HIV infection is by performing a screening test (eg, enzyme-linked immunosorbent assay [ELISA]) and by confirming a positive result with a supplementary test. (medscape.com)
  • The gene coding for the DNA repair enzyme that recognizes and excises 8-oxo-dG is 8-oxoG-DNA glycosylase (OGG1) [ 14 ]. (biomedcentral.com)
  • APRT enzyme activity assay and/or hereditary testing had been performed to verify APRT deficiency generally in most sufferers. (tech-strategy.org)
  • Crystalluria evaluation was performed as previously reported (18 19 APRT enzyme RPS6KA6 activity was assessed in erythrocyte lysates using radiolabeled 14C-adenine within a chromatographic assay (3). (tech-strategy.org)
  • According to the prediction of bioinformatics software of Miranda, we showed that 5′-UTR regions of hsa-miR-124-3p, a mature sequence of human miR-124 precursor, could bind to 3′-UTR region of branched chain amino acid transaminase 1(BCAT1) gene, the enzyme that catalyzes branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth [ 15 , 16 ]. (biomedcentral.com)
  • Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
  • In vitro permeability assays, trans-endothelial invasion assays, in vivo permeability assays and tissue fluorescence staining were conducted to examine vascular permeability. (thno.org)
  • Enhanced ZNF281 transcriptionally regulated CCL2 and CCL5 expression to activate P38 MAPK signaling in endothelial cells. (thno.org)
  • Such data can be used to model intravascular flow, endothelial shear stress, and altered branching geometry such as that which may occur in localized angiogenesis and around tissue infarction and tumors. (gsk-3signals.com)
  • The regulatory mechanism of lncSNHG5 was investigated by RNA sequencing, fluorescent in situ hybridization, cellular fractionation assay, mass spectrometry, RNA pull-down, RNA immunoprecipitation, gene-specific m6A assay, chromatin immunoprecipitation, dual luciferase reporter assay and actinomycin D treatment in CAFs and NFs. (thno.org)
  • Western blotting, RNA-immunoprecipitation, reporter assay, primary MYF culture and ectopic expression rescue were performed to demonstrate the role of CPEB2 in PDGFRα mRNA translation and MYF proliferation. (biomedcentral.com)
  • It uses chemiluminescence for the qualitative detection of human papillomavirus (HPV) DNA in cervical specimens. (cdc.gov)
  • Specimens containing the target DNA hybridize with the HR HPV RNA probe cocktail. (cdc.gov)
  • Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing. (cdc.gov)
  • Downstream next-generation sequencing (NGS) of the syphilis spirochete Treponema pallidum subspecies pallidum (T. pallidum) is hindered by low bacterial loads and the overwhelming presence of background metagenomic DNA in clinical specimens. (cdc.gov)
  • The significance of our research is the development of an SWGA DNA enrichment method that expands the range of clinical specimens that can be directly sequenced to include samples with low numbers of T. pallidum. (cdc.gov)
  • OBJECTIVE: To evaluate the field performance of a multiplex PCR (M-PCR) assay for detection of herpes simplex virus (HSV)-1 and HSV-2, Treponema pallidum (T. pallidum) and Haemophilus ducreyi (H. ducreyi) in genital ulcer disease (GUD) specimens. (cdc.gov)
  • METHODS: GUD M-PCR was performed on 186 remnant specimens, previously collected for HSV testing, by four public health laboratories (PHLs) and the Laboratory Reference and Research Branch (LRRB) at the Centers for Disease Control and Prevention. (cdc.gov)
  • In a survey of 1033 serum specimens for electrolyte analysis in an acute-care hospital, he and an associate found that 53% of the patients had magnesium levels 0.74 mmol/L, while the ordering physicians suspected and ordered magnesium levels specifically in only 10% of patients. (irjs.info)
  • The specimens for assay were snap-frozen and stored in liquid nitrogen until analysis. (mek-inhibitors.com)
  • Qualitative RT-PCR for HCV RNA Test to detect HCV RNA by amplification of viral genetic sequences. (cdc.gov)
  • Quantitative assays for HCV RNA Tests to detect HCV RNA concentration (viral load) by amplification of viral genetic sequences or by signal amplification. (cdc.gov)
  • An RLU measurement equal to or greater than the Cutoff Value indicates the presence of HPV DNA sequences in the specimen. (cdc.gov)
  • An RLU measurement less than the Cutoff Value indicates the absence of the specific HPV DNA sequences tested or HPV DNA levels below the detection limit of the assay. (cdc.gov)
  • Launch Reduction of the DNA mismatch fix (MMR) program by (epi-) hereditary adjustments network marketing leads to an elevated mutation price in brief, repeated sequences tandemly, called microsatellites. (immune-source.com)
  • 8-Oxo-dG in DNA is repaired primarily via the DNA base excision repair pathway. (biomedcentral.com)
  • Mechanistically, we found HepG2 exosomes activated several phopho-kinases and NF-κB signaling pathway in exo-adipocytes. (biomedcentral.com)
  • Iron levels were detected by immunofluorescence staining and colorimetric assays. (stanfordchildrens.org)
  • Our work demonstrates that lncSNHG5 and its downstream signaling ZNF281-CCL2/CCL5 in CAFs play a crucial role in premetastatic niche formation in breast cancer and may serve as potential targets for the diagnosis and treatment of BC metastasis. (thno.org)
  • The DNA damage response (DDR) is a mechanism that protects cells against radiation-induced oxidative DNA damage by causing cell cycle arrest and apoptosis. (researchsquare.com)
  • Ionizing radiation is well known to induce oxidative DNA damage, such as DNA double-strand breaks (DSBs), and consequently trigger the DNA damage response (DDR), including cell cycle arrest and apoptosis. (researchsquare.com)
  • HBV DNA (quantitative viral load) indicates viral burden and viral replication. (medscape.com)
  • To enhance the osteogenic potential of stem cell sheet, we fabricated bone morphogenetic protein 2 ( BMP-2 ) gene-engineered cell sheet using a complex of polyethylenimine-alginate (PEI-al) nanocomposites plus human BMP-2 complementary(c)DNA plasmid, and studied its osteogenesis in vitro and in vivo. (dovepress.com)
  • Tubule formation and three-dimensional sprouting assays and tissue fluorescence staining were conducted to investigate angiogenesis. (thno.org)
  • Serum glutamic-oxaloacetic transaminase, now called AST. (cdc.gov)
  • Functional characterization of the identified placenta-expressed env gene after cloning in a CMV-driven expression vector and transient transfection experiments, demonstrated both fusogenic activity in an ex vivo cell-cell fusion assay and infectivity of pseudotypes. (biomedcentral.com)
  • Tumor behavior is determined by not only the malignant potential of tumor cell itself but also the signals from its microenvironment. (biomedcentral.com)
  • The majority of CRPCs continue to express the androgen receptor (AR) and are dependent on AR signaling for malignant progression [ 3 - 5 ]. (oncotarget.com)
  • In comparison with fiber-rich treatments that increased diversity and promoted Firmicutes and Bacteroidetes populations, resistant starch reduced microbial diversity and fecal pH, led to a bloom in Actinobacteria, and modified Kyoto Encyclopedia of Genes and Genomes orthology (KO) terms pertaining to starch and sucrose metabolism, fatty acid biosynthesis and metabolism, epithelial cell signaling, among others. (biomedcentral.com)
  • Subsequently, coordinated branching morphogenesis and vascular development result in a tree-like structure of epithelial tubules with differentiated airways and saccules. (biomedcentral.com)
  • Consequently, most, if not all, of the hydrogenosomal proteins are encoded by the nuclear DNA, synthesized in the cytosol, and then imported into hydrogenosome via a protein import machinery. (plasignaling.com)
  • It contains the targeting signals and directs the import of most matrix proteins, many inner membrane proteins and some intermembrane space proteins into mitochondria-like organelles (Chacinska et al. (plasignaling.com)
  • Magnesium is integral to the function of adenosine triphosphate and plays a role in a host of enzymatic reactions and transport processes, and in the synthesis of proteins, DNA and RNA. (irjs.info)
  • These data indicate that tuberin deficiency in angiomyolipoma enhances mTOR activation by phosphorylation of S6K and downregulation of protein and mRNA expression of OGG1 resulted in accumulation of oxidized DNA in patients with TSC. (biomedcentral.com)
  • The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. (cusabio.com)
  • The DNA guardian protein p53 plays the most important role in DDR: it promotes DNA repair and the elimination of cells that are unable to repair the damage caused by oxidative stresses, including radiation. (researchsquare.com)
  • Integrin-linked kinase (ILK) is a major structural adaptor protein governing signaling complex formation and cytoskeletal dynamics. (jneurosci.org)
  • The RNA silencing response to viral infection is so robust that all major groups of plant viruses examined to date, including those with DNA genomes, have been shown to encode one or more RNA silencing suppressors (RSSs) which act as pathogenicity determinants (4, 34). (moam.info)
  • Detection and typing of HPV DNA in vaginal swabs (in conjunction with testing of NHANES sera for HPV antibody) will allow evaluation of trends in prevalence of type-specific HPV infection by age, sexual behavior, and race/ethnicity. (cdc.gov)
  • After amplification the samples are typed by hybridization to the typing strips followed by colorimetric detection. (cdc.gov)
  • [ 4 ] Detection of anti-HBs in the serum implies either active or passive immunization that usually persists for life. (medscape.com)
  • In patients with differentiated thyroid cancer treated by surgery and radioactive iodine ablation, serum thyroglobulin(Tg) and 131I whole body scan(WBS) are recognized as being the best cooperative indicators for detection of recurrence or metastasis. (e-enm.org)
  • CONCLUSIONS: The DNA methylation molecular patterns we proposed are an innovative complement to the traditional classification of RCC, which might contribute to precision medicine. (bvsalud.org)
  • In light of these findings, precisely how β1 integrin transduces ECM cues via signaling intermediates, and the mechanism by which this translates to cellular responses, need to be addressed. (jneurosci.org)
  • The authors provided convincing evidence for the heterogeneity of postnatal enthesis growth and the molecular dynamics and signaling networks during enthesis formation. (elifesciences.org)
  • Molecular Diagnostic and Prognostication Assays for the Subtyping of Urinary Bladder Cancer Are on the Way to Illuminating Our Vision. (who.int)
  • With a resolution down to a few micrometers, these images can be used to measure the interbranch segment diameters, branching angles, volume of tissue perfused, and study the vascular anatomic relationships DOCK10 to organ microstructures such as glomeruli in kidney, hepatic lobules in liver, and so on. (gsk-3signals.com)
  • Herein, we aimed to conduct DNA methylation modification patterns in RCC. (bvsalud.org)
  • DNA methylation modification patterns were proposed with an unsupervised clustering algorithm based on 20 DNA methylation regulators. (bvsalud.org)
  • DNA methylation score (DMS) was developed with principal component analysis. (bvsalud.org)
  • RESULTS: Three DNA methylation modification patterns were conducted across RCC patients, namely C1, C2 and C3. (bvsalud.org)
  • DMS enabled the quantification of DNA methylation status individually as an alternative tool for prognostic estimation. (bvsalud.org)
  • DNA methylation is one of the best known epigenetic mechanisms in cancer epigenetics. (biomedcentral.com)
  • DNA methylation involves in the addition of a methyl (CH3) group to DNA with DNA methyltransferases (DNMTs), thereby often modifying gene function through regulation of gene expression [ 7 ]. (biomedcentral.com)
  • In 1964, Groot ( 5 ) described antibodies against OROV in serum samples from primates studied in Magdalena Medio and La Lizama (Colombia) in 1957. (cdc.gov)
  • The resultant RNA: DNA hybrids are captured onto the surface of a microplate well coated with antibodies specific for RNA: DNA hybrids. (cdc.gov)
  • Immobilized hybrids are then reacted with alkaline phosphatase conjugated antibodies specific for the RNA: DNA hybrids, and detected with a chemiluminescent substrate. (cdc.gov)
  • Loss of heterozygosity at the 8-oxoG-DNA glycosylase (OGG1) allele is found in human kidney clear cell carcinoma identifying loss of OGG1 function as a possible contributor to tumorigenesis in the kidney. (biomedcentral.com)
  • This editorial highlights the relevance of interfering in cancer cell progression through the pharmacological manipulation on the cell metabolism of cyclic nucleotides such as cAMP, and on the intracellular Ca2+ signaling, which may avail the reduction of toxic effects promoted by chemotherapy, radiotherapy and immunotherapy, thus decreasing the incidence of interruption in antitumoral treatment. (researchgate.net)
  • On the other hand, we clarified the upstream mechanism regulating miR-124-3p expression in ESCC, which involves in the hypermethylation-silencing regulation mediated by DNA methyltransferase 1(DNMT1), which is of high expression in ESCC tissues and cell lines in the present study. (biomedcentral.com)
  • STR DNA typing did not match any cell lines within three international cell banks. (oncotarget.com)
  • Assays on the cell culture showed that the genetically engineered cells released the BMP-2 for at least 14 days. (dovepress.com)
  • Loss of HBeAg and appearance of anti-HBe in serum is called seroconversion, which is an important clinical event in the course of HBV disease. (medscape.com)
  • Platelet-derived growth factor receptor α (PDGFRα) signaling is crucial for alveolar myofibroblast (MYF) proliferation and its deficiency is associated with risk of BPD, but posttranscriptional mechanisms regulating PDGFRα synthesis during lung development remain largely unexplored. (biomedcentral.com)
  • These assays are 97% specific but cannot distinguish acute from chronic infection. (medscape.com)
  • and mediates signaling between the ECM and the cellular interior. (jneurosci.org)
  • Background Microsatellite lack of stability (MSI) resulting from inactivation of the DNA mismatch fix program (MMR) characterizes a highly immunological subtype of colorectal carcinomas. (immune-source.com)
  • Evaluation of a laboratory-developed multiplex real-time PCR assay for diagnosis of syphilis, herpes and chancroid genital ulcers in four public health laboratories in the USA. (cdc.gov)
  • Glyoxal fixation: an immunohistochemistry assay evaluation. (yale.edu)
  • Mutation evaluation was performed using PCR amplification and sequencing from the gene after obtaining created informed consent in the sufferers (3). (tech-strategy.org)
  • This assay uses Roche Linear Array HPV Genotyping test that is based on HPV L1 consensus polymerase chain reaction (PCR) with biotinylated PGMY09/11 primer sets. (cdc.gov)
  • Despite the deficiency of each primer sets to separate a few species, cluster analysis of combined data sets indicated the ability of rep-PCR technique to separate 26 LGK-974 ic50 out of 27 examined species in highly supported clusters corresponded to the species recognized based on DNA sequence data. (plasignaling.com)
  • characterized by sustained disappearance of serum HCV RNA and normalization of liver enzymes. (cdc.gov)
  • At one end of the spectrum are infected persons who have no signs or symptoms of liver disease and have completely normal levels of serum enzymes, the usual blood test results that indicate liver disease. (txliver.com)
  • At the other end of the spectrum are patients with severe hepatitis C who have symptoms, high levels of the virus (HCV RNA) in serum, and elevated serum enzymes, and who ultimately develop cirrhosis and end-stage liver disease. (txliver.com)
  • The serum sample tested negative for IgM against OROV, GROV, Mayaro virus, and Tacaribe virus by ELISA with whole virus antigen produced in-house. (cdc.gov)
  • All patients had histologically proven recurrence/metastasis and negative 131I WBS results but persistently elevated serum Tg levels. (e-enm.org)
  • Results of HBV serologic markers can be reported qualitatively or quantitatively as international units (IU) or signal per cutoff (s/c) value. (medscape.com)
  • However, in some cases, 131I WBS shows no specific lesions despite elevated serum Tg. (e-enm.org)
  • The lncSNHG5-ZNF281-CCL2/CCL5 signaling axis plays an essential role in inducing premetastatic niche formation to promote BC metastasis. (thno.org)
  • Moreover, decreased proliferating ability in KO MYFs due to insufficient PDGFRα expression was rescued by ectopic expression of CPEB2, suggesting an important role of CPEB2 in upregulating PDGFRα signaling for pulmonary alveologenesis. (biomedcentral.com)
  • Liquid drop of DNA libraries reveals total genome information. (yale.edu)
  • Studied predictive use of mucin 1 (KL-6) serum level as a biomarker in development of bronchopulmonary dysplasia in preterm infants. (cusabio.com)
  • Since defective pulmonary PDGFR signaling is a key feature of human BPD, CPEB2 may be a risk factor for BPD. (biomedcentral.com)
  • It also includes biotinylated β-globin primers as an internal control for sample amplification. (cdc.gov)
  • The DNA extracts are used in the Digene hc2 HPV DNA Test. (cdc.gov)
  • This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
  • Robert Whang known that serum measurements, while practicable, were not adequate reflections of total body stores and took to measuring magnesium in mononuclear cells. (irjs.info)
  • 1% Tween 20, and cells have been blocked overnight rocking at four C in 5% standard goat serum diluted in TBST. (narturalproduct.com)
  • This was demonstrated by a co-culture fusion assay between hamster A23 cells transduced with an expression vector for ASCT2 and A23 cells transduced with syncytin-Ory1 . (biomedcentral.com)
  • HBV can be present in hepatocytes in two forms: as replicating virus, leading to production of infectious particles, or as a nonreplicative form after integrating into the host DNA. (medscape.com)