Dna fragments dna recombinant. Medical search. Frequent questions

The first factor of plasmid fitness is its ability to replicate DNA. (wikipedia.org)
Isolation of clonable DNA (genomic libraries, cDNA synthesis) cloning vectors (plasmids, bacteriophages, cosmids) plasmid incompatibility and control of copy number. (up.ac.za)
We produce plasmid DNA, recombinant proteins and Ab fragments up to kg scale for clinical and commercial human uses. (eurogentec.com)
Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. (scielo.br)
Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and each daughter cell receives at least one copy of the plasmid. (addgene.org)
By the 1970s the combined discoveries of restriction enzymes, DNA ligase, and gel electrophoresis allowed for the ability to move specific fragments of DNA from one context to another, such as from a chromosome to a plasmid. (addgene.org)
The combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can be used to study DNA fragments of interest, such as genes. (addgene.org)
A 570 bp DNA fragment of probe was produced by digesting the recombinant plasmid with RNA disturbance. (mingsheng88.org)

Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
Over the next 100 years, many significant discoveries lead to the conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. (addgene.org)
These findings culminated in the central dogma of molecular biology, that proteins are translated from RNA, which is transcribed from DNA. (addgene.org)
Willem P.C. Stemmer of Affymax Research Institute in Palo Alto, California, published a paper in the Proceedings of the National Academy Science in 1994, describing a technique for rapidly shuffling and recombining DNA in the laboratory that appears to generate recombinant proteins with greatly improved performances. (i-sis.org.uk)
The Ubl SUMO regulates a growing number of recognized proteins involved in the cell cycle, DNA repair, the stress response, nuclear transport, transcription, and signal transduction. (enzolifesciences.com)
Scientists found that the precise arrangement (or sequence) of nitrogen bases A, C, G. and T on the DNA strand is the recipe that codes for the manufacture of specific chemical compounds, namely proteins. (faqs.org)
Neither did antibody to the recombinant 37,000 dalton protein react with any treponemal proteins purified by fibronectin affinity chromatography. (bmj.com)
Single strand annealing proteins (SSAPs) like Redβ initiate homologous recombination by annealing complementary DNA strands. (nature.com)
By mechanism, DNA annealing proteins are divided into ATPases capable of strand invasion (RecA, RAD51) and single strand annealing proteins (SSAPs) that do not utilize ATP 4 . (nature.com)
As opposed to single strand (ss) DNA binding proteins such as SSB and RPA, which protect and occlude ssDNA from recombination, RAD52/Redβ SSAPs promote recombination and share several biochemical similarities. (nature.com)
They are weak ssDNA binding proteins with no affinity for double-stranded (ds) DNA. (nature.com)
The giver DNA is separated from molecular components of the cell such as proteins, RNA, lipoids and saccharides. (free-essays.us)
In transduction, the recombinant molecule is combined with phage caput and tail proteins from one cell to another utilizing bacteriophage vector. (free-essays.us)
Three CAV viral proteins VP1, VP2 and VP3 was separately cloned and expressed in recombinant E. coli . (biomedcentral.com)
The purified recombinant CAV VP1, VP2 and VP3 proteins were then used as antigens in order to evaluate their reactivity against chicken sera using indirect ELISA. (biomedcentral.com)
To carry out the further antigenic characterization of the immunorelevant domains of the VP2 and VP3 proteins, five recombinant VP2 subunit proteins (VP2-435N, VP2-396N, VP2-345N, VP2-171C and VP2-318C) and three recombinant VP3 subunit proteins (VP3-123N, VP3-246M, VP3-366C), spanning the defined regions of VP2 and VP3 were separately produced by an E. coli expression system. (biomedcentral.com)
The results of these ELISAs using truncated recombinant VP2 and VP3 subunit proteins as coating antigen showed that VP2-345N, VP2-396N and VP3-246M gave good immunoreactivity with CAV-positive chicken sera compared to the other subunit proteins. (biomedcentral.com)
Purified recombinant VP2-396N and VP2-345N subunit proteins, which span defined regions of VP2, were demonstrated to have good antigenicity and higher sensitivities than VP3-246M and were able to recognize CAV-positive chicken serum using an ELISA assay. (biomedcentral.com)
These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)

Plasmids: These are generally circular extrachromosomal DNA molecules that replicate and are transmitted independent from chromosomal DNA. (wikipedia.org)
This technique allows efficient creation of scarless recombinant plasmids at many, but not all, positions in a vector. (neb.com)
These fragments are then inserted into cloning vectors , such as bacterial plasmids or bacteriophages, which transfer the recombinant DNA to suitable host cells, such as the bacterium E. coli. (encyclopedia.com)
This led us to construct recombinant DNA plasmids containing the encoding sequence of HBsAg and the same construction fused with the SEQDEL sequence responsible for ER sorting. (scielo.br)
Plasmids are fragments of double-stranded DNA that typically carry genes and can replicate independently from chromosomal DNA. (addgene.org)
Plasmids that are used most commonly in the field of recombinant DNA technology have been optimized for their use of studying and manipulating genes. (addgene.org)
Typically plasmids contain the minimum essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments can be inserted. (addgene.org)
Larger pieces of DNA such as entire plasmids, viral and bacterial genomes can also be recombined. (i-sis.org.uk)

Alternatively, complementary DNA is inserted into the vectors, or 'naked' DNA fragments can be taken up directly by a host bacterium from its medium (this is less efficient than vector transfer). (encyclopedia.com)
What precautions have they taken with regard to the recombinants and vectors used, to prevent them from being released into the environment? (i-sis.org.uk)
The second step is to insert the insulin gene into the vector.Viruses, liposomes (hollow spheres of fat molecules formed in solution), andplasmids (circular forms of DNA) are common vectors. (faqs.org)
The addition of foreign DNA in the form of recombinant DNA vectors generated by molecular cloning is the most common method of genetic engineering. (penelopethemovie.com)

The reducing cytoplasmic environment prevents the formation of the disulfide bonds normally required for the stable folding of full-length antibodies and antibody fragments, including single-chain variable fragment (scFv) antibodies 6,7 . (jove.com)
We have resources to develop human antibodies using diversity of large combinatorial antibody libraries using phage display technology and to produce recombinant antibodies and antibody fragments in small scale using bacterial and mammalian expression systems. (lu.se)

A viable vector must be able to replicate together with the DNA fragments it carries. (wikipedia.org)
Figure 2 Identification of recombinant vector by PCR. (wjgnet.com)
This creative technique uses the 3' → 5' exo activity of T4 DNA Polymerase to create overhangs with complementarity between the vector and insert. (neb.com)
This allows the exonuclease activity of T4 DNA Polymerase to proceed and generate the complementary overlaps between insert and vector. (neb.com)
One of these interesting approaches is the use of nicking DNA endonucleases (NiDE) to create the complementary overlaps used to anneal the vector and insert. (neb.com)
The recombinants are cloned into a vector and introduced into bacteria for rapid screening. (i-sis.org.uk)
A genomic library consisting of partially digested 10 to 20 kilobase pair fragments of Treponema pallidum deoxyribonucleic acid (DNA) was constructed using bacteriophage lambda EMBL-3 as the vector. (bmj.com)
2) The insertion of an isolated donor DNA fragment into a vector genome and (3) The growth of a recombinant vector in an appropriate host. (penelopethemovie.com)
Purified DNA was ligated in to the pMD18-T vector (TaKaRa, Japan), and recombinant clones had been digested. (woofahs.com)

Characterization (polymerase chain reaction, nucleic acid sequencing) and mutagenisis of cloned DNA fragments. (up.ac.za)
These fragments are heated up to separate the two strands and made to re-anneal (pair up again) in the presence of a DNA polymerase, an enzyme that synthesize DNA. (i-sis.org.uk)
With fragments re-annealing in a random way, the DNA polymerase can switch templates many times in the course of reassembly, and that is how different recombinants are generated. (i-sis.org.uk)
The reaction combination contained 0.1 mM dNTP, 0.5 mM of every degenerate primer and 1.0 U of HiFi-Taq DNA polymerase (TransGen Biotech, Guangzhou, China) in a complete level of 25 l. (woofahs.com)
The complementary DNA is prepared from messenger RNA of retroviruses utilizing particular enzyme contrary RNA polymerase. (free-essays.us)

Figure 1 10 g/L agarose gel electrophoresis of cagA DNA fragment amplified by PCR from coccoidal H pylori . (wjgnet.com)
Restriction fragments can be analyzed using techniques such as gel electrophoresis or used in recombinant DNA technology. (richardvigilantebooks.com)
c) Name the stain used to visualize DNA fragments in Gel electrophoresis. (biopassionate.net)

Ranibizumab is a humanised monoclonal antibody fragment produced in Escherichia coli cells by recombinant DNA technology. (medicines.org.uk)
This recombinant Human monoclonal antibody Fab Fragment (VR0261) is specific for MERS-COV S recognition. (creative-biolabs.com)

Suppose that the base sequence T-G-G-C-T-A-C-T on a DNA molecule carries the instruction "make insulin. (faqs.org)
Other scientists were quick torecognize that this provided a means of cutting a large DNA molecule into well-defined smaller fragments. (faqs.org)
The two DNA molecule sites can crossbreed to each other through their reconciliation gluey ends to organize a recombinant molecule. (free-essays.us)
In transmutation, recombinant DNA molecule enters into bacteriums cells. (free-essays.us)
Chicken anemia virus (CAV) is the member of the genus Gyrovirus of the family Circoviridae , and the genome consists of a circular single-stranded 2.3 kb DNA molecule [ 1 ]. (biomedcentral.com)

Looking to isolate genomic DNA? (thermofisher.com)
Four ospC clonal complexes dence of genetic endemism, recent migration events, and (A, B, I, and K groups) were found to be more likely than recombinant genomic types. (cdc.gov)

In the experiment on retroviral genomes, 6 mouse leukaemia viruses (MLV) were recombined in a single round, giving a library of 5 million replicating recombinant viruses. (i-sis.org.uk)
Among them were completely new viruses that infect Chinese Hamster Ovary cells, which none of the original MLV was capable of infecting, and recombinant viruses that were 30 to 100 times more stable than the parental strains, and hence much better for gene therapy. (i-sis.org.uk)
Some of the recombinant viruses and bacteria could well be lethal. (i-sis.org.uk)
Are the recombinant viruses and bacteria, or their DNA, strictly contained within the laboratory? (i-sis.org.uk)
They observed that when viruses entered the bacteria, the viral DNA was cut into small pieces and inactivated by certain bacterial enzyme. (faqs.org)
SARS-CoV-2 Delta-Omicron Recombinant Viruses, United States. (cdc.gov)

In 1973, an American biochemist Paul Berg often referred to as the father ofgenetic engineering developed a method for joining the DNA of two different organisms, a monkey virus known as SV40 and a second virus known as lambda phage. (faqs.org)
One recombinant phage was examined further and contained an insert encoding a prominent treponemal 37,000 dalton protein. (bmj.com)

We used recombinant congenic mouse strains to map asthma susceptibility genes on mouse chromosome 17. (rug.nl)
To put this into practice, he devised a method for reassembling genes from random gene fragments in the test tube. (i-sis.org.uk)
Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
DNA sequence alignment analysis and the forecast-analysis of protein structure indicated that these seven antimicrobial peptide genes were not homologous to any other antimicrobial peptide genes in GenBank. (ukessays.com)

Fragment Size-Based Enrichment of Viral Sequences in Plasma Cell-Free DNA. (cdc.gov)
rVSV-ZEBOV is a live-attenuated recombinant viral vaccine. (msdmanuals.com)

You will use recombinant DNA technology to examine structure-function relationships in a bacterial enzyme. (edu.au)

Whether you prefer organic reagents, filter columns, or magnetic beads, our products for Isolation of DNA fragments are designed for sensitive, scalable extraction and are compatible with a range of downstream applications. (thermofisher.com)
Invitrogen DNA purification kits provide easy, fast and safe methods for isolation of DNA fragments that yield exceptional results. (thermofisher.com)
Which cell-free DNA isolation kit is right for you? (thermofisher.com)
Routine isolation of DNA fragments from agarose gel is made easier with DNA gel extraction kits from Thermo Fisher Scientific. (thermofisher.com)
Techniques in chemistry enable isolation and purification of cellular components, such as DNA, but practically this isolation is only feasible for relatively short DNA molecules. (addgene.org)

The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)
We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)

The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)
Ad26.ZEBOV is a recombinant DNA vaccine based on adenovirus type 26 (Ad26). (msdmanuals.com)
MVA-BN-Filo is a recombinant DNA vaccine based on modified vaccinia Ankara (MVA). (msdmanuals.com)
and Shingrix, GlaxoSmithKline, [GSK]) are licensed and recommended in the United States for adults 50 years and older (recombinant vaccine) 26 and 60 years of age and older (live attenuated vaccine). (cdc.gov)

Transposons: These are DNA sequences that can move and replicate in different parts of a cell's genome. (wikipedia.org)
DNA transposons: These are transposons that move directly from one position to another in the genome using a transposase to cut and stick at another locus. (wikipedia.org)
Lane 2: Amplifi-cation cagA gene from coccoid H pylori genome DNA by PCR. (wjgnet.com)
In order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence of a few hundred or few thousand basepairs from the entire human genome. (addgene.org)
Digesting the human genome with restriction enzymes would yield about two million DNA fragments, which is far too many to separate from each other for the purposes of isolating one specific DNA sequence. (addgene.org)
In order to establish infection, the virus must reverse transcribe its single stranded RNA genome into double stranded DNA, traverse the cytoplasm and cross the nuclear membrane, after which it integrates into the host chromosome ( Bukrinsky, 2004 ). (elifesciences.org)

Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)

We show that digestion and re-annealment of single chain variable fragment (scFv) coding DNA is possible using CuPhen. (rsc.org)
Here, we describe a protocol to screen a recombinant library of single-chain variable fragment (scFv) antibodies for antigen-binding and proper cytoplasmic folding simultaneously. (jove.com)

Recombinant congenic strains are series of strains which have inherited one or more chromosomal fragments from a donor strain and all other DNA from the background strain. (rug.nl)

and a shorter sequence paired to a longer one will 'prime' the synthesis of DNA using the longer sequence as a template, until the complete double stranded DNA is restored. (i-sis.org.uk)

The cohesive ends generated by the restriction endonuclease made it possible to join double helical DNA fragments from different sources by complementary base-pairing. (faqs.org)
Recently the research workers developed complementary DNA from chemical procedure. (free-essays.us)

gene cloning ( DNA cloning ) The production of exact copies ( clones ) of a particular gene or DNA sequence using genetic engineering techniques. (encyclopedia.com)
Different variants of a gene or a DNA sequence, represented by the different colours are fragmented and reassembled to generate millions of recombinants all at once. (i-sis.org.uk)
DNA in the cells of the islets of Langerhans in the pancreas would normally contain that base sequence since the islets are the regions in which insulin is produced in mammals. (faqs.org)
If a way could be found to insert the base sequence into the DNA of bacteria, for example, then those bacteria would be capable of manufacturing insulin. (faqs.org)
This procedure of section is called a DNA palindrome which explains the both strands have same nucleotide sequence but antiparallel placement. (free-essays.us)
Multilocus sequence typing (MLST) is the use of DNA dorferi ( 9 , 10 ). (cdc.gov)

In the 1950s, largely as the result of the pioneering work of James Watson (1928-) and Francis Crick (1916-), scientists discovered the structure of deoxyribonucleic acid (DNA) molecules and how DNA stores and transmit genetic information. (faqs.org)
The Deoxyribonucleic acid can be broken into set of limitation fragments harmonizing to the place of the limitation sites utilizing physically shearing it or by enzymatically digesting it which endonucleases. (free-essays.us)
Deoxyribonucleic acid (DNA). (who.int)

Seven gene fragments for three types of antimicrobial peptides cecropin, sarcotoxin and stomoxyn were acquired. (ukessays.com)
Bioinformatic analysis was done to predict the secondary structure and three-dimensional structure of these seven gene fragments that may exist. (ukessays.com)

Joined fragments have 4 nicks that are repaired by E.coli during transformation. (neb.com)

However, one of the factors that make it difficult and raise the price of production in every system consists in the purification process of the recombinant antigen. (scielo.br)
Phen = 1,10-phenanthroline) was applied under Fenton-type conditions against a recombinant antibody fragment specific for prostate-specific antigen (PSA) and compared against traditional DNA shuffling using DNase I for the generation of recombinant mutagenesis libraries. (rsc.org)

You will split the protein into fragments using molecular techniques, and re-constitute the enzyme to restore its activity. (edu.au)
The DNA of the gene is digested with the enzyme deoxyribonuclease 1 (DNAse 1) into random 10 to 50 bp (base-pair) fragments. (i-sis.org.uk)
When this recombinant is 'backcrossed' to the parental gene using the same DNA shuffling technique, which he later calls, 'molecular breeding', an enzyme that was 32 000 times as effective as the wild type enzyme was obtained. (i-sis.org.uk)
A restriction enzyme, restriction endonuclease, or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. (richardvigilantebooks.com)
A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. (richardvigilantebooks.com)

DNA Ligase. (penelopethemovie.com)
Ligase is chiefly used to fall in two DNA fragments and the procedure called as ligation. (free-essays.us)
The recombinant molecules do non hold covalent bond on rear sugar medieties, the DNA ligase used to seal the sugar molecules and anchor which creates phosphodiester bonds at the functions.DNA ligase widely used in lab and it is derived from the bactriophage T4. (free-essays.us)

The silica membrane technology captures DNA fragments without the need for pH adjustment. (thermofisher.com)
This obstacle has been overcome by the field of recombinant DNA technology, which enables the preparation of more managable (i.e., smaller) DNA fragments. (addgene.org)
Genetic engineering is the alteration of an organism's genotype using recombinant DNA technology to modify an organism's DNA to achieve desirable traits. (penelopethemovie.com)
44. a) Write any four tools used in recombinant DNA technology. (biopassionate.net)

Methionine can then be used to produce SAM, the body's universal methyl donor, which participates in several other key metabolic pathways, including the methylation of DNA and myelin. (medscape.com)

In Europe, 'tolerated releases' are allowed, and this include not only transgenic wastes consisting of dead bacteria and cells (with plenty of transgenic DNA), but also certain living bacteria deemed, by the companies concerned, not to pose a threat to health or the environment. (i-sis.org.uk)

These genetic elements are cleaved at four single stranded sites in DNA by transposase. (wikipedia.org)
Consequently, their cutscreated short single stranded tails on the ends of each fragment, called "sticky ends" or cohesive ends. (faqs.org)
After digestion of a DNA with certain Restriction enzymes, the ends left have one strand overhanging the other to form a short (typically 4 nt) single-stranded segment. (richardvigilantebooks.com)

These enzymes usually do not cut straight acrossthe two strands of DNA, but in a staggered fashion. (faqs.org)

The recombinant DNA techniques were foremost demonstrated in 1972, the parent field of molecular biological science was about entirely an academic field of research. (free-essays.us)
As recombinant DNA engineering exposed its practical potency during the 1970s, support for molecular biological science section began to utilize bit by bit more from the private instead than the public sector ( Susan. (free-essays.us)

One of the biggest mileposts, readying of HUMAN INSULIN utilizing recombinant DNA engineering. (free-essays.us)

Since 1987 two recombinant vaccines produced into Saccharomices cerevisiae cells by Sharp & Dhome and Smith Kline Beechmam have been utilized for HBV immunization. (scielo.br)

Results indicate recombinant library generation in this manner may generate novel clones-not accessible through the use of DNase I-with CuPhen producing highly PSA-specific binding antibodies identified by surface plasmon resonance. (rsc.org)

Invitrogen DNA gel extraction kits are designed to isolate DNA fragments from agarose gel in 15-30 minutes. (thermofisher.com)
The amplified fragment was retrieved within a 1% agarose gel and purified using the Gel Removal Package (Omega, USA). (woofahs.com)

Direct and indirect methods for the identification of recombinant organisms. (up.ac.za)

We then started to map asthma susceptibility in the Lmr series of recombinant congenic and subcongenic strains. (rug.nl)

This copy can be obtained from a natural source (like DNA in islets of Langerhans cells), or it can be manufactured artificially in the laboratory. (faqs.org)
b) Mention any two methods of introducing alien DNA into host cells. (biopassionate.net)
I'm fascinated by liquid biopsy - the ability to find pieces of DNA of cancer cells in blood. (medscape.com)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)

Ligation of DNA fragments, modification of DNA end and different ligation strategies. (up.ac.za)

Fragments of DNA can be inserted by recombinant DNA techniques. (wikipedia.org)
You will use three-dimensional protein modelling, DNA techniques, and protein techniques to complete this project. (edu.au)
Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)

Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
High recovery of DNA fragments is critical to the success of the entire workflow. (thermofisher.com)

Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

Even if a protein is very complex in structure, which could be a limitation in recombinant, because your body makes it, it knows how to do it. (medscape.com)

Lyme disease is a multisystem infection, with infl am- tinents, and isolates of this clone were uniform in DNA se- matory complications that commonly affect the skin, joints, quences, which suggests a recent trans-oceanic migration. (cdc.gov)

Even non-homologous DNA, from totally unrelated species can be recombined. (i-sis.org.uk)

This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)

New DNA is obtained by either isolating and copying the genetic material of interest using recombinant DNA methods or by artificially synthesising the DNA. (penelopethemovie.com)

The methylotrophic yeast species Komagataella phaffii (synonym: Pichia pastoris) is widely used as a host for recombinant protein production. (ipaqt.org)

DNA fingerprinting, with IS 6110 as a probe, has been used successfully to trace transmission of M. tuberculosis in outbreaks, confirm laboratory cross-contamination, and identify risk factors for disease among populations of patients with TB ( 1 ). (cdc.gov)

This results in two recombinant duplexes containing the semi conserved transposable element flanked by the previous 5 to 9 base pair overhang. (wikipedia.org)
So the results obtained with DNA shuffling seem out of this world. (i-sis.org.uk)

Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. (neb.com)

Anatomy7

Organisms18

Diseases1

Chemicals and Drugs49

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes64

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care1

Plasmid8

Proteins19

Plasmids8

Vectors4

Antibodies and antibody fragments2

Vector9

Polymerase5

Electrophoresis3

Monoclonal antibody2

Molecule5

Genomic2

Viruses6

Phage2

Genes4

Viral2

Bacterial1

Isolation5

Escherichia2

Vaccine4

Genome6

Antibody libraries1

ScFv2

Chromosomal1

Double-stranded1

Complementary2

Sequence6

Deoxyribonucleic acid3

Gene fragments2

Coli1

Antigen2

Enzyme5

Ligase3

Technology4

Donor1

Transgenic1

Single stranded3

Strands1

Molecular2

Insulin1

Vaccines1

Generate1

Agarose gel2

Organisms1

Strains1

Cells4

Ligation1

Techniques3

Workflow2

Biological1

Limitation1

Commonly1

Homologous1

Cell-free DNA4

Methods1

Host1

Probe1

Results2

Cloning1