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  • fragments
  • We assessed ciprofloxacin (CIP)-induced chromosomal DNA breakage in single-cell Escherichia coli by direct visualization of the DNA fragments that diffused from the nucleoid obtained after bacterial lysis in an agarose microgel on a slide. (beds.ac.uk)
  • high
  • Four E. coli strains with identified mechanisms of reduced sensitivity to CIP were assessed using this procedure and produced DNA fragmentation levels that were inversely related to MIC dose, except those with very high MIC dose. (beds.ac.uk)
  • damage
  • DNA damage started to be detectable at the MIC dose. (beds.ac.uk)
  • At a dose of 1 μg/ml of CIP, DNA damage was visualized clearly immediately after processing, and the DNA fragmentation increased progressively with the antibiotic incubation time. (beds.ac.uk)
  • The level of DNA damage was much higher when the bacteria were taken from liquid LB broth than from solid LB agar. (beds.ac.uk)
  • CIP treatment produced a progressively slower rate of DNA damage in bacteria in the stationary phase than in the exponentially growing phase. (beds.ac.uk)
  • repair
  • The magnitude of DNA repair was inversely related to CIP dose and was noticeable after incubation with CIP at 0.1 μg/ml but scarce after 10 μg/ml. (beds.ac.uk)