• On the basis of sequences from peptides of BTF3, we have now cloned two complementary DNAs, one for a protein (BTF3a) with all the characteristics of purified BTF3, and one for a shorter protein (BTF3b) lacking the first 44 residues of BTF3a and which is transcriptionally inactive, despite its ability to bind RNA pol II. (nih.gov)
  • Five UDP glucuronosyltransferases (UGT) were synthesized from complementary DNAs expressed in COS 7 cells and were tested for their capacities to glucuronidate a range of 2-acetylaminofluorene and benzo( a )pyrene-hydroxylated metabolites. (aacrjournals.org)
  • Thus, 3n7nz A ( 1 ) has the potential for use as a fluorescent probe for structural studies of DNAs/RNAs including the detection of single-base alterations in target DNA sequences. (rsc.org)
  • The ID (inhibitor of differentiation or DNA binding) helix-loop-helix proteins are important mediators of cellular differentiation and proliferation in a variety of cell types through regulation of gene expression. (isharonline.org)
  • DNA that has been synthesized from mRNA by the action of reverse transcriptase. (drugs.com)
  • This enzyme, which naturally occurs in retroviruses, operates on a single strand of mRNA, generating its complementary DNA based on the pairing of RNA base pairs (A, U, G and C) to their DNA complements (T, A, C and G, respectively). (wikipedia.org)
  • A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA (Reverse transcriptase requires this double-stranded segment as a primer to start its operation. (wikipedia.org)
  • Reverse transcriptase is added, along with deoxynucleotide triphosphates (A, T, G, C). This synthesizes one complementary strand of DNA hybridized to the original mRNA strand. (wikipedia.org)
  • Here we screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoadenine (nA) and 2-thiothymine (sT) to generate structure-free DNA that is fully accessible to complementary probes. (jefferson.edu)
  • We identified and characterized a human orthologue of Rif1 protein, which in budding yeast interacts in vivo with the major duplex telomeric DNA binding protein Rap1p and negatively regulates telomere length. (isharonline.org)
  • Of the 26 TCMs investigated, all but two can be classified as non-compliant on the grounds of DNA, toxicology and heavy metals, or a combination thereof. (blogspot.com)
  • Subcellular distribution of signal recognition particle and 7SL-RNA determined with polypeptide-specific antibodies and complementary DNA probe. (ucsf.edu)
  • A double stranded DNA produced from the messenger RNA synthesis in a reaction catalyzed by an enzymes reverse transcriptase. (biology-online.org)
  • Metabarcoding is a rapid method of biodiversity assessment that combines two technologies: DNA based identification and high-throughput D. (blogspot.com)
  • DNA, Complementary" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • In 1980, Aviv initiated and started "Biotechnology General Corp.", the first Israeli Biotechnology company focusing on Recombinant DNA (genetic engineering), which exists to this day. (wikipedia.org)
  • The multi-tiered approach outlined in this study provides a much-needed auditing toolkit that should swiftly form the basis of best-practice pharmacovigilance across the CAM [complementary and alternative medicines] sector. (blogspot.com)
  • Such vectors allow for self-replication, inside the cells, and potentially integration in the host DNA. (wikipedia.org)
  • The researchers also found one of the herbal medicines for sale had trace amounts of snow leopard DNA in it. (blogspot.com)
  • In addition they found DNA from pit vipers, frogs and trace amounts of cat and dog DNA. (blogspot.com)
  • During the late 1970s, Aviv entered the field of Recombinant DNA (genetic engineering) and applied research, and produced a microorganism (E. coli) containing the gene for Bovine Growth Hormone. (wikipedia.org)