• Residual hepatitis B virus (HBV) DNA can be detected following the resolution of acute HBV infection. (semanticscholar.org)
  • There is however, a potentially confounding issue in that PCR can generate larger targets from collections of small DNA fragments, a phenomenon known as PCR recombination. (biomedcentral.com)
  • Primers incorporated mutations (underlined) to distinguish PCR fragments from input HBVayw DNA. (biomedcentral.com)
  • DNA plasmid! (shapeways.com)
  • We found the presence of plasmid DNA in strain T88-56 of the Japanese pear pathotype of Alternaria alternata , which causes black spot of certain cultivars of Japanese pear by producing host-specific AK-toxin. (genetics.org)
  • The plasmid, designated pAAT56, was identified to be an ∼5.4-kilobase (kb) circular molecule by electron microscopic observation and restriction endonuclease mapping. (genetics.org)
  • Herein, we developed a sensitive PCR-free electrokinetic-based sensor combined with a customized bis-peptide nucleic acid (bis-PNA) and gamma-PNA (γ-PNA) probes immobilized on beads, for the detection of mtDNA point mutations and sequence-specific supercoiled plasmid DNA at the picomolar range. (springer.com)
  • Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA & RNA cleanup. (neb.com)
  • The paper, Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification, published in the June issue of Genome Research 11:1095-1099, describes a rapid, scalable method for the amplification of circular DNA directly from bacterial cells or from viral plaques, generating high quality templates for use in DNA sequencing, probe generation or cloning. (freethesaurus.com)
  • Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD. (nih.gov)
  • By tethering either DNA or PcrA to a microscope coverslip surface, unwinding of both linear and natural circular plasmid DNA by PcrA/RepD was followed in real-time using total internal reflection fluorescence microscopy. (nih.gov)
  • The single-molecule data show that PcrA, in combination with RepD, can unwind plasmid lengths of DNA in a single run, and that PcrA is active as a monomer. (nih.gov)
  • Visualization of circular plasmid DNA unwinding using immobilized PcrA. (nih.gov)
  • plasmid using EcoRI-PvuI (New Britain Biolabs) [16 17 Briefly HepG2 cells were seeded at a density of 2-3 mil cells in 100-mm-diameter Petri dishes and transfected twenty four hours later with 0.5 μg to at least one 1 μg of digested HBV DNA. (cura-cancer.com)
  • In a normal plasmid DNA preparation, multiple forms of DNA may be present, and gel from the electrophoresis of the plasmids would normally show a main band which would be the negatively supercoiled form, while other forms of DNA may appear as minor fainter bands. (wikipedia.org)
  • One key observation in support of a double-helical structure is that the individual single strands of small circular viral and plasmid DNA are inseparable under the usual types of denaturation conditions, supporting a plectonemic, i.e., twisted structure whose strands are topologically locked together. (wikipedia.org)
  • In many organisms, one strand of DNA in the plasmid comprises heavier nucleotides (relatively more purines: adenine and guanine). (wikipedia.org)
  • As pan-kDNA most closely resembles a DNA plasmid, it may be the ancestral form of kDNA. (wikipedia.org)
  • Thus, circular DNA in the circulation is a previously unexplored pool of nucleic acids that could complement miRNAs and linear DNA for diagnosis and for intercellular communication. (aacrjournals.org)
  • A number of factors can affect the migration of nucleic acids: the dimension of the gel pores (gel concentration), size of DNA being electrophoresed, the voltage used, the ionic strength of the buffer, and the concentration of intercalating dye such as ethidium bromide if used during electrophoresis. (wikipedia.org)
  • Unlike type IIA topoisomerases, type IIB topoisomerases cannot simplify DNA topology (see below), but they share several structural features with type IIA topoisomerases. (wikipedia.org)
  • In addition, type IIA topoisomerases are able to simplify DNA topology (Rybenkov Science 1997), while type IIB topoisomerases do not (Corbett Journal of Molecular Biology, 2006). (wikipedia.org)
  • DNA Structure and Topology at Molecular Biochemistry II: The Bello Lectures. (wikipedia.org)
  • (physiciansweekly.com)
  • That DNA, called chromosomal DNA, is found in each cell's nucleus and contains genetic instructions needed to carry out biological functions. (hotstarshop.com)
  • In contrast to the previously discovered circular RNAs composed of spliced-gene exons and functioning primarily as miRNA (micro RNA) sponges in the cell cytoplasm, ciRNAs act as transcriptional enhancers in the nucleus. (icr.org)
  • The nucleus of senescent cells is characterized by senescence-associated heterochromatin foci (SAHF) and DNA segments with chromatin alterations reinforcing senescence (DNA-SCARS). (wikipedia.org)
  • The second DNA assembly technique is in vitro recombination method based on single-strand overhangs generated by single-strand exonucleases or incomplete PCR (Polymer Chain Reaction), typically represented by OE-PCR (Overlap Extension-PCR). (biomedcentral.com)
  • Residual hepatitis B virus (HBV) DNA can be detected following the resolution of acute HBV infection. (semanticscholar.org)
  • HBV was identified as a new DNA virus in the 1960s, followed a couple of decades later by the discovery of the flavivirus Hepatitis C. HBV was first identified in the lab as the "Australia agent" by Blumberg and colleagues in the blood of an Aboriginal transfusion patient. (wikipedia.org)
  • We observed 36 HIV-infected patients to evaluate whether the presence of tandem 2-long terminal repeat circular unintegrated HIV-1 DNA (2-LTR) in peripheral blood mononuclear cells (PBMC) at baseline was associated with acceleration of HIV disease. (ovid.com)
  • Additionally, there are descriptions of the presence of papillomavirus DNA in the blood and in the peripheral blood mononuclear cells. (wikipedia.org)
  • Agarose gel is easy to cast, has relatively fewer charged groups, and is particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. (wikipedia.org)
  • The lower sulphate content of low EEO agarose, particularly low-melting point (LMP) agarose, is also beneficial in cases where the DNA extracted from gel is to be used for further manipulation as the presence of contaminating sulphates may affect some subsequent procedures, such as ligation and PCR. (wikipedia.org)
  • Viral burden quantification in ES patients has been limited to measurements of total DNA in PBMC, and estimates of Infectious Units per Million cells (IUPM). (plos.org)
  • There appears to be no significant difference in the level of total HIV DNA between cells from ES patients and patients on HAART. (plos.org)
  • The role models for the protein and DNA structures in the N-terminal domain are taken from the comparable role models for protein and DNA in the protamine-DNA complex in sperm cells. (oalib.com)
  • Cells can also be induced to senesce via DNA damage in response to elevated reactive oxygen species (ROS), activation of oncogenes and cell-cell fusion, independent of telomere length. (wikipedia.org)
  • Consistent with this, telomerase-immortalised cells continued to age (according to the epigenetic clock) without having been treated with any senescence inducers or DNA-damaging agents, re-affirming the independence of the process of epigenetic ageing from telomeres, cellular senescence, and the DNA damage response pathway. (wikipedia.org)
  • Cells are lysed and DNA is extracted from the cell lysate. (wikipedia.org)
  • While researchers are beginning to better understand how circular DNA functions in humans, much is still unknown. (hotstarshop.com)
  • When diagramed, the resulting structure looks like the letter D. The D-loop was first discovered in 1971 we researchers noticed that many DNA in the mitochondria they were examining under microscope contained a short segment that was tripled stranded. (wikipedia.org)
  • However, for constructions of most circular DNA, there are two contradictions in the ligation/assembly and transformation steps. (biomedcentral.com)
  • the second contradiction is that a successful transformation of a ligation/assembly product requires a relatively high DNA concentration again. (biomedcentral.com)
  • In the first round of adapter ligation, a right and left adapter (Ad1) is attached to the right and left flanks of the fragmented DNA, and the DNA is amplified by PCR (Figure 3). (wikipedia.org)