SubunitSubunitsMRNAPOLGInhibitionGeneProteinsReplicationEnzymeBinding and cleavageMechanismsEnzymesMechanismEpsilonCleavageHeterodimerActivityReactionMoleculesMutationMutationsSequencesPhosphorylationInteractionGlycosylaseMethylationNHEJAssaySubstratesMolecular2022CellularComplexCytosolicPolyclonalExonucleaseInhibitorStructuresRepairTargetsStrandsAssaysDamageReactions
Subunit18
  • DNA polymerase alpha catalytic subunit is an enzyme that in humans is encoded by the POLA1 gene. (wikipedia.org)
  • This gene encodes the p180 catalytic subunit of DNA polymerase α-primase. (wikipedia.org)
  • The Pol α complex (pol α-DNA primase complex) consists of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the small and the large primase subunits PRIM1 and PRIM2 respectively. (wikipedia.org)
  • Reference: Functional expression and characterization of the Epstein-Barr virus DNA polymerase catalytic subunit. (neb.com)
  • A recombinant baculovirus containing the complete sequence for the Epstein-Barr virus (EBV) DNA polymerase catalytic subunit, BALF5 gene product, under the control of the baculovirus polyhedrin promoter was constructed. (neb.com)
  • The DNA polymerase and the exonuclease activities of the EBV DNA polymerase catalytic subunit were sensitive to ammonium sulfate in contrast to those of the polymerase complex purified from EBV-producing lymphoblastoid cells, which were stimulated by salt. (neb.com)
  • Furthermore, the template-primer preference for the polymerase catalytic subunit was different from that for the polymerase complex. (neb.com)
  • These observations strongly suggest that the presence of EBV DNA polymerase accessory protein, BMRF1 gene product, does influence the enzymatic properties of EBV DNA polymerase catalytic subunit. (neb.com)
  • DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase related kinase family is a well-known player in repairing DNA double strand break through non-homologous end joining pathway. (molcells.org)
  • DNA-dependent protein kinase catalytic subunit (DNA-PKcs), encoded by PRKDC gene, is a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, which also includes the two DNA damage repair proteins, ataxia telangiectasia mutated (ATM) and ATM and Rad3-related protein (ATR). (molcells.org)
  • DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a key component of the non-homologous end-joining (NHEJ) pathway, is involved in DNA double-strand break repair, immunocompetence, genomic integrity, and epidermal growth factor receptor signaling. (amegroups.org)
  • The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is the key component of the non-homologous end-joining (NHEJ) pathway for DNA double-strand break (DSB) repair and is required for cellular resistance to ionizing radiation (IR) ( 1 , 2 ). (amegroups.org)
  • Conclusion: the conserved areas of the human DNA polymerase e catalytic subunit require highly conserved structure for optimal function and are not involved in breast cancer progression. (lml.com.ly)
  • We found that DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and mammalian target of rapamycin (mTOR) complex 2 (mTORC2) were both required for UVB-induced Akt Ser-473 phosphorylation in keratinocytes. (biomedcentral.com)
  • Protein phosphatase 2A (PP2A) is an enzymatic complex containing three subunits: a catalytic subunit, a scaffolding subunit, and one of several regulatory subunits responsible for dictating substrate specificity of the enzyme (42). (grandlacs-med-journal.com)
  • The common A467T mutation in the human mitochondrial DNA polymerase (POLG) compromises catalytic efficiency and interaction with the accessory subunit. (musc.edu)
  • Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
  • We have previously reported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to mediate the cellular response to DNA double strand break (DSB) caused by DNA-damaging metabolites. (cdc.gov)
Subunits1
  • 2003. Evolutionary connection between the catalytic subunits of DNA-dependent RNA polmerases and eukaryotic RNA-dependent RNA polymerases. (icr.org)
MRNA6
  • This leads to altered mRNA splicing and decreased expression of POLA1 protein to a level that does not impair DNA replication. (wikipedia.org)
  • We modified the Dz13 DNAzyme (which showed promise of a therapeutic agent in preclinical studies by targeting the c-jun mRNA 14 ) and preprogrammed it into a library of Boolean logic gates where only upon specific conditions, Dz13 regains its catalytic activity. (nature.com)
  • 8-oxo-dGuo expressions as well as protein and mRNA expressions of DNA repair enzyme hOGG1 and antioxidant defenses (CAT, GCLC, GPx, Nrf2, and MnSOD) in nonneoplastic epidermis of control and BCC tissues were also determined. (hindawi.com)
  • Sequence-specific cleavage of Huntington mRNA by catalytic DNA. (bcm.edu)
  • Treatment with NU7026 did not alter the production of reactive oxygen species and oxidative stress by hydroquinone but repressed the protein level of DNA-PKcs and blocked the induction of the kinase mRNA and protein expression by hydroquinone. (cdc.gov)
  • Furthermore, Reimand said, the team identified APOBEC (apolipoprotein B mRNA editing catalytic polypeptide-like) enzyme-driven SGMs, "which may help decipher the role of APOBECs in tumorigenesis and progression. (medscape.com)
POLG1
  • DNA Polymerase γ (gamma), POLG, is the sole mitochondrial replicative DNA polymerase. (mitomap.org)
Inhibition9
  • Various anti-DNA-PKcs strategies have been developed and tested in preclinical studies to exploit the benefit of DNA-PKcs inhibition in sensitization of radiotherapy and in combined modality therapy with other antitumor agents. (amegroups.org)
  • In this article, we review the association between DNA-PKcs and cancer development and discuss current approaches and mechanisms for inhibition of DNA-PKcs. (amegroups.org)
  • The future challenges are to understand how DNA-PKcs activity is correlated with cancer susceptibility and to identify those patients who would most benefit from DNA-PKcs inhibition. (amegroups.org)
  • Inhibition of DNA-PKcs activity via a small interfering RNA or a kinase inhibitor results in mitosis delay, abnormal spindle formation, and chromosome misalignment. (amegroups.org)
  • Inhibition of DNA-PKcs activity via its inhibitor NU7026, a dominant-negative kinase-dead mutation, RNA interference (RNAi) or gene depletion led to the attenuation of UVB-induced Akt Ser-473 phosphorylation. (biomedcentral.com)
  • Finally, we found that both DNA-PKcs and SIN1 were associated with apoptosis resistance of UVB radiation, and inhibition of them by NU7026 or genetic depletion significantly enhanced UVB-induced cell death and apoptosis. (biomedcentral.com)
  • IMPORTANCE Replication intermediates and ends of viral DNA genomes can be recognized by the cellular DNA damage response (DDR) network as DNA damage whose repair may Lycopodine lead to inhibition of virus replication. (grandlacs-med-journal.com)
  • Furthermore, the ability of E4orf4, when expressed alone, to accumulate at DNA damage sites and to kill cancer cells is attenuated by chemical inhibition of PARP-1. (grandlacs-med-journal.com)
  • Inhibition of DNA-PKcs by NU7026 markedly potentiated the apoptotic and growth inhibitory effects of hydroquinone in proerythroid leukemic K562 cells in a dose-dependent manner. (cdc.gov)
Gene10
  • Methods: 157 DNA samples from human breast cancers and 133 DNA control samples (lymph node, placenta) were analysed with the PCR-SSCP methodology for mutation in the highly conserved regions of the polymerase e gene. (lml.com.ly)
  • The analysis involved 14 different primer pairs for the large unit of polymerase e gene and 35% of the reading frames of the catalytic unit was scanned. (lml.com.ly)
  • The optimal performance of the SSCP methodology was confirmed in the exonuclease I region of the polymerase epsilon gene in 92 cases of breast cancer, which did not show evidence of mutation in SSCP analysis or in DNA sequencing. (lml.com.ly)
  • They are developing atomic-level models that explain how chemical energy is transduced into force and motion, and how dynamic assemblies control DNA replication, gene expression, chromosome superstructure, and other essential nucleic-acid transactions. (hopkinsmedicine.org)
  • DNA methylation is one of a number of modes of epigenetic gene regulation. (ca.gov)
  • Collectively, our data show that DNA methylation is essential and instructive for deposition of specific histone modifications across regulatory regions, which together influences gene expression patterns in mESCs. (ca.gov)
  • The TERT gene encodes the catalytic sub-unit of telomerase, which polymerizes telomeric DNA. (uab.edu)
  • This study highlights an important contribution for Pcif1 in gene expression and physiology of the organism independently of any catalytic activity. (cea.fr)
  • The lack of catalytic activity of Pcif1 in Drosophila reveals a new mechanism of gene expression regulation. (cea.fr)
  • The catalytic activities of these two COMT variants, expressed in E. coli, were similar, but the Met-108 enzyme was more thermolabile already at physiological temperature (37 °C). The reported existence of a common polymorphism of the human COMT gene coding for a thermolabile low activity, COMT(L), and a thermostable high activity, COMT(H), is discussed in light of the different thermostability of the two enzyme forms. (lu.se)
Proteins9
  • Here we highlight some of the latest evidence where DNA-PKcs has been shown to localize not only in the nucleus but also in the cytoplasm, phosphorylating various proteins involved in cellular metabolism and cytokine production. (molcells.org)
  • Genes that suppress carcinogenesis have been classified as gatekeepers that regulate cellular proliferation and cell death and as caretakers that are primarily encode DNA repair proteins required for the maintenance of genome integrity ( 15 ). (amegroups.org)
  • Dr. Berger's team is also studying the nucleic acid-dependent motors, with a particular focus on a variety of DNA- and RNA-dependent motor proteins. (hopkinsmedicine.org)
  • We have previously determined the structure of various domains within this family and characterized how these proteins heterodimerize, how they bind to DNA but the catalytic reaction remains elusive and what determines the preferential heterodimer formation is also not known. (uu.nl)
  • These studies elucidate the structure-function relationship of these DNA repair proteins. (uu.nl)
  • Cytosine C5 methylation is an important epigenetic control mechanism in a wide array of eukaryotic organisms and generally carried out by proteins of the C-5 DNA methyltransferase family (DNMTs). (mdpi.com)
  • Basic ParABS systems comprise two proteins, ParA and ParB, and a centromere-like, cis-acting DNA element called parS . (biorxiv.org)
  • This expression is strictly dependent on the synthesis of messenger RNAs transcripts from the DNA molecule and their subsequent translation into proteins by the ribosomes. (cea.fr)
  • This study demonstrates the importance to not restrict the function of enzymes to their sole catalytic role in the living world and the interest of thoroughly examining the evolution of proteins in eukaryotes in order to discover unsuspected or hidden mechanisms of action. (cea.fr)
Replication8
  • Pol α is responsible for the initiation of DNA replication at origins of replication (on both the leading and lagging strands) and during synthesis of Okazaki fragments on the lagging strand. (wikipedia.org)
  • In addition to its role during DNA replication, POLA1 plays a role in type I interferon activation. (wikipedia.org)
  • DNA replication involves producing new copies of DNA molecules. (answers.com)
  • Activation of DNA-PKcs by UV is dependent on ATR (Ataxia telangiectasia mutated and Rad3 related) kinase and is important for replication stress [ 12 ]. (biomedcentral.com)
  • The oxidative modification of guanine leads to formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGuo), which finally can pair with adenine and cytosine during DNA replication, resulting in GC-TA mutations found to be associated with development of BCC [ 8 ]. (hindawi.com)
  • Dr. Berger's laboratory is also exploring whether the protein machinery responsible for initiating DNA replication can be exploited as a novel anti-cancer target. (hopkinsmedicine.org)
  • During DNA replication, recombination and DNA repair, double-stranded DNA frequently forms three- or four-way junctions, bubbles, flaps or broken ends with single-stranded extensions. (uu.nl)
  • 2 Viruses enter into a host cell for replication (the process of producing two identical replicas of RNA, ribonucleic acid or DNA, deoxyribonucleic acid) and take over the cell's biological machinery. (icr.org)
Enzyme3
  • Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1), previously thought to be a backup enzyme for uracil-DNA glycosylase, has recently been shown to excise 5-hydroxyuracil (hoU), 5-hydroxymethyluracil (hmU) and 5-formyluracil (fU) bearing an oxidized group at ring C5 as well as an uracil. (elsevierpure.com)
  • The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA-DNA base-pairing to target foreign DNA in bacteria. (nature.com)
  • Furthermore, impaired DNA repair capacity was suggested to associate with enhanced susceptibility to cancer and deficiency in DNA repair enzyme, human 8-oxoguanine DNA N-glycosylase 1(hOGG1), a key enzyme responsible for 8-oxo-dGua repair, may also be involved in carcinogenesis [ 11 ]. (hindawi.com)
Binding and cleavage1
  • We show that both binding and cleavage of DNA by Cas9-RNA require recognition of a short trinucleotide protospacer adjacent motif (PAM). (nature.com)
Mechanisms2
  • In this work we investigated the point of interaction between E4orf4 and the DDR as well as the mechanisms by which E4orf4 inhibits DNA damage signaling. (grandlacs-med-journal.com)
  • 2022. Probing the mechanisms of two exonuclease domain mutators of DNA polymerase ε. (nih.gov)
Enzymes3
Mechanism2
  • In the present study, we used site-directed mutagenesis to construct a series of mutants of human SMUG1 (hSMUG1), and tested their activity for uracil, hoU, hmU, fU and other bases to elucidate the catalytic and damage-recognition mechanism of hSMUG1. (elsevierpure.com)
  • Thus, the results presented here demonstrate a novel mechanism by which E4orf4 targets and inhibits DNA damage signaling through an association with PARP-1 for the benefit of the virus and impacting E4orf4-induced cancer cell death. (grandlacs-med-journal.com)
Epsilon1
  • Highly conserved region of the catalytic unit of human DNA polymerase epsilon in breast cancer. (lml.com.ly)
Cleavage1
  • Here we use single-molecule and bulk biochemical experiments to determine how Cas9-RNA interrogates DNA to find specific cleavage sites. (nature.com)
Heterodimer2
  • In response to DSB formation, DNA-PKcs is recruited to DSBs by the DNA end-binding Ku70/80 heterodimer and is rapidly phosphorylated at multiple serine and threonine residues ( 1 , 2 ). (amegroups.org)
  • Their catalytic function however depends on heterodimer formation with the non-catalytic family members. (uu.nl)
Activity8
  • Tyrosyl-DNA phosphodiesterase 2 (TDP2) is a 5'-tyrosyl DNA phosphodiesterase important for the repair of DNA adducts generated by non-productive (abortive) activity of topoisomerase II (TOP2). (rcsb.org)
  • These results indicate that the 3'-to-5' exonuclease activity associated with the EBV DNA polymerase (T. Tsurumi, Virology 182:376-381, 1991) is an inherent feature of the polymerase catalytic polypeptide. (neb.com)
  • have reported that increased DNA-PKcs activity can suppress HR repair in G1 phase of cells through increasing RBX1 protein expression, which prompts the neddylation and activity of cullin 1, a key component of the Skp1-Cullin1-F-box ubiquitin 3 ligase, consequently mediating ubiquitination and degradation of EXO1. (molcells.org)
  • Clinical studies indicate that expression and activity of DNA-PKcs is correlated with cancer progression and response to treatment. (amegroups.org)
  • Thus, deficiency in DNA-PKcs activity results in a severe combined immunodeficiency (SCID) phenotype in mammals ( 3 - 5 ). (amegroups.org)
  • These effects require DNMT catalytic activity. (ca.gov)
  • Any process that activates or increases the frequency, rate or extent of DNA-directed DNA polymerase activity. (mcw.edu)
  • During evolution, this protein naturally lost its catalytic activity in Drosophila where Pcif1 is, like its human counterpart, expressed in the nucleus and associated with the C-terminal domain of RNA polymerase (RNA Pol II). (cea.fr)
Reaction4
  • The molecular details of the nucleotidyl transferase reaction have remained speculative, as strategies to trap catalytic intermediates for structure determination utilize substrates lacking the primer terminus 3'-OH and catalytic Mg2+, resulting in an incomplete and distorted active site geometry. (rcsb.org)
  • A catalytic colorimetric detection scheme that incorporates a DNA-based hybridization chain reaction into gold nanoparticles was designed and tested. (boisestate.edu)
  • This application of a DNA reaction network to gold nanoparticle-based colorimetric detection enables highly-sensitive, field-deployable, colorimetric readout systems capable of detecting a variety of biomolecules. (boisestate.edu)
  • Recently we have implemented an in vitro assay that functionally resembles the DNA repair reaction and assays to study protein-protein and protein-DNA interactions. (uu.nl)
Molecules3
  • The reduction in POLA1 expression is accompanied by marked reduction in cytosolic RNA:DNA hybrid molecules and a concomitant hyperactivation of the IRF3 pathway, with consequent overproduction of type I interferons. (wikipedia.org)
  • These results reveal how Cas9 uses PAM recognition to quickly identify potential target sites while scanning large DNA molecules, and to regulate scission of double-stranded DNA. (nature.com)
  • After a molecule of DNA replicates, two double-stranded molecules are formed. (answers.com)
Mutation1
  • UVB-induced complexation between DNA-PKcs and mTORC2 components was also abolished by NU7026 and DNA-PKcs mutation. (biomedcentral.com)
Mutations4
  • A direct link between DNA DSBs, genomic instability, and cancer is evidenced the fact that many cancer-predisposition syndromes in humans characterized by genomic instability are caused by mutations in DSB-responsive genes ( 16 , 17 ). (amegroups.org)
  • The aim: to study the potential mutations associated with conserved exon regions of the catalytic unit of human polymerase e in human breast cancer tissue samples. (lml.com.ly)
  • Our study shows that tobacco smoking signatures in DNA generate these harmful protein-truncating mutations that contribute to the development of cancer and its increasing complexity over time," senior author Jüri Reimand, PhD, Ontario Institute for Cancer Research, Toronto, Canada, told Medscape Medical News . (medscape.com)
  • We have known for decades that smoking causes DNA mutations that in turn cause cancer. (medscape.com)
Sequences1
  • Non-target DNA binding affinity scales with PAM density, and sequences fully complementary to the guide RNA but lacking a nearby PAM are ignored by Cas9-RNA. (nature.com)
Phosphorylation7
  • In vivo , DNA-PKcs phosphorylation has been examined at the Thr2609 cluster region and Ser2056. (amegroups.org)
  • DNA-PKcs phosphorylation at the Thr2609 cluster region is particularly significant since it is critical for DSB repair and radioresistance ( 6 - 9 ). (amegroups.org)
  • This phosphorylation is regulated by the ataxia telangiectasia mutated (ATM) and the Rad3-related (ATR) kinases in response to various genotoxic stresses, making it a critical regulatory element of DNA-PKcs ( 6 , 10 ). (amegroups.org)
  • DNA-PKcs phosphorylation at Ser2056, on the other hand, is primarily mediated by autophosphorylation, making it an excellent marker to monitor DNA-PKcs activation in vivo ( 11 ). (amegroups.org)
  • DNA-PKcs activation, as monitored by DNA-PKcs phosphorylation, is physically and functionally associated with mitotic spindle formation. (amegroups.org)
  • Significantly, we discovered that DNA-PKcs was associated with SIN1 in cytosol upon UVB radiation, and this complexation appeared required for Akt Ser-473 phosphorylation. (biomedcentral.com)
  • Taken together, these results strongly suggest that DNA-PKcs-mTORC2 association is required for UVB-induced Akt Ser-473 phosphorylation and cell survival, and might be important for tumor cell transformation. (biomedcentral.com)
Interaction2
  • Here, we present the results of an X-ray absorption spectroscopy study of a mercury sensor which illustrates the nature of the Hg-DNA interaction. (illinois.edu)
  • We show that E4orf4 interacts with PARP-1 and explore the consequences of this interaction during virus infection and under conditions of nonviral DNA damage. (grandlacs-med-journal.com)
Glycosylase2
  • Single probe-based catalytic quantum dot FRET nanosensor for human alkyladenine DNA glycosylase detection. (bvsalud.org)
  • Human alkyladenine DNA glycosylase (hAAG) is essential for repairing alkylated and deaminated bases, and it has become a prospective diagnosis biomarker and a therapeutic target for disease treatment . (bvsalud.org)
Methylation4
  • Reversible Regulation of Promoter and Enhancer Histone Landscape by DNA Methylation in Mouse Embryonic Stem Cells. (ca.gov)
  • Here, we profile the DNA methylome, transcriptome, and global occupancy of histone modifications (H3K4me1, H3K4me3, H3K27me3, and H3K27ac) in a series of mouse embryonic stem cells (mESCs) with varying DNA methylation levels to study the effects of DNA methylation on deposition of histone modifications. (ca.gov)
  • DNA methylation promotes H3K27me3 deposition at bivalent promoters, while opposing H3K27me3 at silent promoters. (ca.gov)
  • DNA methylation also reversibly regulates H3K27ac and H3K27me3 at previously identified tissue-specific enhancers. (ca.gov)
NHEJ2
  • DNA-PKcs and the NHEJ pathway are also essential for V(D)J recombination during T and B cell lymphocyte development. (amegroups.org)
  • Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. (lu.se)
Assay1
  • Figure 1: DNA curtains assay for target binding by Cas9-RNA. (nature.com)
Substrates3
  • Here, we present a crystal structure of a precatalytic complex of a DNA polymerase with bound substrates that include the primer 3'-OH and catalytic Mg2+. (rcsb.org)
  • Dr. Berger's team has solved structures of topoisomerase targets in complex with target DNA substrates and various drugs. (hopkinsmedicine.org)
  • Here, the catalytic number (V(max)) of S-COMT was somewhat higher than that of MB-COMT for all four substrates. (lu.se)
Molecular1
  • In this study, we used a new, small molecule, a selective inhibitor of DNA-PKcs, 2-(morpholin-4-yl)-benzo[h]chomen-4-one (NU7026), as a probe to analyze the molecular events and pathways in hydroquinone-induced DNA DSB repair and apoptosis. (cdc.gov)
20223
  • Under ARS 13-3728(A), which was amended in May 2022, it is unlawful for a person to purchase, solicit, advertise, possess or sell a used catalytic converter or any nonferrous parts of a catalytic converter. (maricopacountyattorney.org)
  • Prior to May 2022, no law made it unlawful to possess a used catalytic converter in Arizona. (maricopacountyattorney.org)
  • 2022. Ribonucleotide incorporation by eukaryotic B-family DNA replicases and its consequences. (nih.gov)
Cellular2
  • Moreover, while there are several DNA-PK inhibitors currently being evaluated in the clinical trials in attempt to increase the efficacy of radiotherapy or chemotherapy, multiple functions and subcellular localization of DNA-PKcs in various types of cells may further complicate the effects at the cellular and organismal level. (molcells.org)
  • Excessive reactive oxygen species (ROS) generated by UVR have been shown to contribute to malignant transformation of keratinocytes into cancerous cells including BCC probably through oxidative DNA damage, defects in DNA repair, and interference with cellular signaling [ 6 , 7 ]. (hindawi.com)
Complex5
  • We studied CTP- and parS F -modulated ParA F -ParB F complex assembly, in which DNA-bound ParA F -ATP dimers are activated for ATP hydrolysis by interacting with two ParB F N-terminal domains. (biorxiv.org)
  • Magnetic-tweezers experiments showed that CTP promotes multiple ParB F loading onto parS F -containing DNA, generating condensed partition complex-like assemblies. (biorxiv.org)
  • and results in the formation of a large nucleo-protein complex (the partition complex) around the parS site on the DNA to be partitioned. (biorxiv.org)
  • Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. (lu.se)
  • The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the KU p70/p86 dimer (g22p1/g22p2) and PRKDC. (lu.se)
Cytosolic1
  • The expressed EBV DNA polymerase catalytic polypeptide was purified from the cytosolic fraction of the recombinant virus-infected insect cells. (neb.com)
Polyclonal1
  • Here, we describe an association of E4orf4 Rabbit Polyclonal to PIK3CG with the DNA damage sensor poly(ADP-ribose) polymerase 1 (PARP-1). (grandlacs-med-journal.com)
Exonuclease1
  • The purified protein exhibited both DNA polymerase and 3'-to-5' exonuclease activities, which were neutralized by the anti-BALF5 protein-specific antibody. (neb.com)
Inhibitor1
  • Meanwhile, this DNA-PKcs-SIN1 complexation by UVB was dependent on epidermal growth factor receptor (EGFR) activation, and was disrupted by an EGFR inhibitor (AG1478) or by EGFR depletion. (biomedcentral.com)
Structures1
  • Comparison with two new structures of DNA polymerase beta lacking the 3'-OH or catalytic Mg2+ is described. (rcsb.org)
Repair3
  • Under the direction of County Attorney Rachel Mitchell, this office has focused on preventing catalytic converter thefts by hosting etching events in partnership with local police departments and Midas Auto Repair. (maricopacountyattorney.org)
  • TERT expression is associated with metastasis from thin primaries, exhausted CD4+ T cells in melanoma and with DNA repair across cancer entities. (nih.gov)
  • Taken together, the findings reveal a central role of DNA-PKcs in hydroquinone-induced hematotoxicity in which it coordinates DNA DSB repair, cell cycle progression, and apoptosis to regulate the response to hydroquinone-induced DNA damage. (cdc.gov)
Targets1
  • One area of research focus is the study of DNA topoisomerases, which are well-established, clinically-validated targets of several anti-cancer agents. (hopkinsmedicine.org)
Strands4
  • Gold nanoparticles were functionalized with thiol-modified DNA strands capable of undergoing hybridization chain reactions. (boisestate.edu)
  • How many individual DNA strands exist after one molecule of DNA has been replicated? (answers.com)
  • DNA is made of 2 strands so when it replicates the strands separate and 2 new strands complete the old strands. (answers.com)
  • This results in a total of four strands of DNA. (answers.com)
Assays1
  • Competition assays provide evidence that DNA strand separation and RNA-DNA heteroduplex formation initiate at the PAM and proceed directionally towards the distal end of the target sequence. (nature.com)
Damage4
  • When E4orf4 is expressed alone, it associates with poly(ADP-ribose) (PAR) chains and is recruited to DNA damage sites in a PARP-1-dependent manner. (grandlacs-med-journal.com)
  • This study illustrated an involvement of oxidative DNA damage and changes in antioxidant defenses in BCC by conducting a case-control study (24 controls and 24 BCC patients) and assessing urinary 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxo-dGuo), plasma antioxidant defenses including catalase (CAT), glutathione peroxidase (GPx), NQO1, and total superoxide dismutase (SOD) activities, and glutathione (GSH) levels before surgery and 1 month after surgery. (hindawi.com)
  • Herein, we concluded that BCC patients were associated with oxidative DNA damage and depletion of antioxidant defenses and surgical removal of BCC correlated with improved redox status. (hindawi.com)
  • 8-oxo-dGuo has also been recognized to be the most abundant and potentially mutagenic if not substantially repaired and has thus been developed as a sensitive and stable biomarker for evaluating the degree of oxidative DNA damage. (hindawi.com)
Reactions2
  • Gelelectrophoresis was performed to compare DNA hybridization reactions in catalytic and direct aggregation schemes, and the product formation was confirmed in the catalytic aggregation scheme at low levels of target concentrations. (boisestate.edu)
  • The idea was that if you attach small catalytic units to the branches of large dendrimers, the catalysts can take part in homogeneous reactions but, being part of a larger entity, are more easily recoverable,' says Russell Morris, the group's leader. (chemistryworld.com)