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DNA-Binding ProteinsDNAMolecular Sequence DataBase SequenceTranscription FactorsProtein BindingBinding SitesPromoter Regions, GeneticAmino Acid SequenceTranscription, GeneticDNA, Single-StrandedNuclear ProteinsElectrophoretic Mobility Shift AssayEscherichia coliMutationNucleic Acid ConformationGene Expression RegulationCell LineCarrier ProteinsCell NucleusPoly(A)-Binding ProteinsDNA, ViralModels, MolecularRepressor ProteinsDNA HelicasesBacterial ProteinsTransfectionDNA ReplicationHypertrophyProtein Structure, TertiaryViral ProteinsCloning, MolecularRecombinant Fusion ProteinsReplication Protein ASequence Homology, Amino AcidTrans-ActivatorsTranscriptional ActivationZinc FingersHeLa CellsOligodeoxyribonucleotidesProteinsPlasmidsRNACells, CulturedRecombinant ProteinsPancreatic NeoplasmsRNA, MessengerNeoplasmsPoly TTacrolimus Binding ProteinsKineticsSequence AlignmentEscherichia coli ProteinsDNA PrimersRNA-Binding ProteinsProtein ConformationPoly(A)-Binding Protein ICCAAT-Enhancer-Binding ProteinsSaccharomyces cerevisiae ProteinsDimerizationElectrophoresis, Polyacrylamide GelNeoplasms, Cystic, Mucinous, and SerousNFI Transcription FactorsDNA FootprintingLeucine ZippersBinding, CompetitiveMolecular WeightHelix-Turn-Helix MotifsInsulin-Like Growth Factor Binding ProteinsRegulatory Sequences, Nucleic AcidY-Box-Binding Protein 1DNA, BacterialConsensus SequenceSaccharomyces cerevisiaeFungal ProteinsMutagenesis, Site-DirectedChromatography, AffinityHomeodomain ProteinsSequence DeletionMacromolecular SubstancesNeoplasm ProteinsDNA, ComplementarySignal TransductionDNA, FungalGene Expression Regulation, BacterialSkin NeoplasmsCyclic AMP Response Element-Binding ProteinHeterogeneous-Nuclear RibonucleoproteinsDNA PrimaseGene ExpressionOligonucleotidesSubstrate SpecificityFatty Acid-Binding ProteinsStructure-Activity RelationshipPolydeoxyribonucleotidesUltraviolet RaysPhosphorylationMutagenesisHelix-Loop-Helix MotifsOctamer Transcription Factor-1
HumansGenesHypoxiaProto-OncogeneApoptosisSitu hybridizationMethylationAmino acidsGenotypeMutationKinaseBenign neoplasmsProgressionOxidative stressDamageMiceMRNAEnzymeCellsCarcinomaTranscriptionInteractsMalignantGene ExpressionDouble-strandedNuclearRegulateBreastMismatch repairCancersMutationsChemotherapyUbiquitin
Humans2
  • Methyl-CpG-binding domain protein 4 is a protein that in humans is encoded by the MBD4 gene. (wikipedia.org)
  • We recently used the allo-restricted strategy to circumvent immunologic tolerance to a ubiquitously expressed tumor-associated protein, MDM2, and raised high avidity CTLs in humans and in mice. (birmingham.ac.uk)
Genes8
  • While other DNA repair genes, such as MGMT and MLH1, are often evaluated for epigenetic repression in many types of cancer,[citation needed] epigenetic deficiency of MBD4 is usually not evaluated, but might be of importance in such cancers as well. (wikipedia.org)
  • Alterations in genes with potential clinical utility are observed in more than three quarters of the cases and included members of the PI3-kinase and homologous DNA repair pathways. (nih.gov)
  • Cellular DNA-binding proteins encoded by the rel gene (GENES, REL). (childrensmercy.org)
  • Pre-treatment mRNA expression of 280 DNA repair (DNAR) genes was tested for association with histopathological regression at surgery, disease-free survival (DFS) and overall survival (OS). (ox.ac.uk)
  • Genes newly defined as targets of the VHL/hypoxia pathway (conditionally downregulated by VHL in normoxic cells) include aminopeptidase A, collagen type V, alpha 1, cyclin G2, DEC1/Stra13, endothelin 1, low density lipoprotein receptor-related protein 1, MIC2/CD99, and transglutaminase 2. (ox.ac.uk)
  • Cancers occur when genetic mutations build up in critical genes, specifically those that control cell growth and division (proliferation) or the repair of damaged DNA. (medlineplus.gov)
  • The EGFR and KRAS genes each provide instructions for making a protein that is embedded within the cell membrane. (medlineplus.gov)
  • Targeted therapy is a cancer treatment that targets specific genes and proteins responsible for cancerous cell growth and survival. (yashodahospitals.com)
Hypoxia2
  • Using cDNA arrays and serial analysis of gene expression, we found that hypoxia induces up-regulation of BNIP3 and its homologue, Nip3-like protein X. Analysis of human carcinoma cell lines showed that they are hypoxically regulated in many tumor types, as well as in endothelial cells and macrophages. (ox.ac.uk)
  • Regulation was hypoxia inducible factor-1-dependent, and hypoxia inducible factor-1 expression was suppressed by von Hippel-Lindau protein in normoxic cells. (ox.ac.uk)
Proto-Oncogene3
  • Proto-Oncogene Proteins c-rel" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • This graph shows the total number of publications written about "Proto-Oncogene Proteins c-rel" by people in this website by year, and whether "Proto-Oncogene Proteins c-rel" was a major or minor topic of these publications. (childrensmercy.org)
  • Below are the most recent publications written about "Proto-Oncogene Proteins c-rel" by people in Profiles. (childrensmercy.org)
Apoptosis4
  • In the present study, we found that Girdin is involved in DNA damage-induced cancer cell apoptosis. (fujita-hu.ac.jp)
  • Bcl2/adenovirus EIB 19kD-interacting protein 3 (BNIP3) is a cell death factor that is a member of the Bcl-2 proapoptotic family recently shown to induce necrosis rather than apoptosis. (ox.ac.uk)
  • Several data suggest that SIAH proteins may have a role in tumor suppression and apoptosis. (hal.science)
  • When DNA becomes damaged, p53 helps determine whether the DNA will be repaired or the cell will self-destruct ( undergo apoptosis ). (medlineplus.gov)
Situ hybridization2
  • In-situ hybridization for Dll4 was performed on serial sections and compared with protein expression. (ox.ac.uk)
  • This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
Methylation5
  • DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. (wikipedia.org)
  • MBD4 mRNA expression is reduced in colorectal neoplasms due to methylation of the promoter region of MBD4. (wikipedia.org)
  • Overall, our results suggest that hPaf1/PD2 in association with MLL1 regulates methylation of H3K4 residues, as well as interacts and regulates nuclear shuttling of chromatin remodeling protein CHD1, facilitating its function in pancreatic cancer cells. (nebraska.edu)
  • Blood cell DNA methylation biomarkers in preclinical malignant pleural mesothelioma: The EPIC prospective cohort. (who.int)
  • Genetic Regulation of DNA Methylation Yields Novel Discoveries in GWAS of Colorectal Cancer. (who.int)
Amino acids4
  • Human MBD4 protein has 580 amino acids with a methyl-CpG-binding domain at amino acids 82-147 and a C-terminal DNA glycosylase domain at amino acids 426-580. (wikipedia.org)
  • The complete 2.3-kb nucleotide sequence of the cDNA was found to contain an open reading frame that could encode a protein of 731 amino acids. (elsevierpure.com)
  • The predicted amino acid sequence contains a high concentration of charged amino acids in the carboxy terminal quarter of the molecule, three guanosine 5′-triphosphate (GTP)-binding protein motifs, and a consensus nuclear localization signal. (elsevierpure.com)
  • The human SOX11 protein is 46.7 kDa and has 441 amino acids. (nordiqc.org)
Genotype1
  • In recent studies Haflinger horses with a homozygous genotype for a missense variant in the DDB2 gene (damage specific DNA binding protein 2) had a significant increased risk of developing ocular SCC. (bvsalud.org)
Mutation1
Kinase1
  • The transcription factor FOSL1 (also known as Fra-1), which is found in basal-like breast cancers but not in the luminal subtype, stimulated the transcription of miR-221/222, and the abundance of these miRNAs decreased with inhibition of the epidermal growth factor receptor (EGFR) or MEK (mitogen-activated or extracellular signal-regulated protein kinase kinase), placing miR-221/222 downstream of the RAS pathway. (nih.gov)
Benign neoplasms2
  • Pituitary adenomas (PA) are commonly occurring benign neoplasms. (cancerindex.org)
  • the induction of benign neoplasms may in some circumstances (see Section 9) contribute to the judgement that the exposure is carcinogenic. (who.int)
Progression2
  • Previously, we reported that SIAH-1 induces the degradation of Kid (KIF22), a chromokinesin protein implicated in the normal progression of mitosis and meiosis, by the ubiquitin proteasome pathway. (hal.science)
  • Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
Oxidative stress2
  • Oxidative stress produces DNA damage and also induces responses, which could help the cell to restore the initial equilibrium. (hindawi.com)
  • This review describes recent advances in the role of human HMGB proteins and other proteins interacting with them, in cancerous processes related to oxidative stress, with special reference to ovarian and prostate cancer. (hindawi.com)
Damage10
  • An esophageal cancer cell line that exhibited high Girdin expression showed a marked sensitivity to UV-mediated DNA damage compared to a line with low Girdin expression. (fujita-hu.ac.jp)
  • When transcriptional activation of endogenous Girdin was mediated by an engineered CRISPR/Cas9 activation system, sensitivity to DNA damage increased in both stationary and migrating HeLa cancer cells. (fujita-hu.ac.jp)
  • Des études récentes ont montré que les chevaux Haflinger présentant un génotype homozygote pour un variant faux-sens dans le gène DDB2 (damage specific DNA binding protein 2) avaient un risque significativement plus élevé de développer un CE oculaire. (bvsalud.org)
  • Using the comet assay to detect DNA damage, we find that reoxygenation induced significant amounts of DNA damage. (ox.ac.uk)
  • These phosphorylations were then maintained in response to reoxygenation-induced DNA damage in an ATM-dependent manner. (ox.ac.uk)
  • The reoxygenation-induced p53 serine 15 phosphorylation was inhibited by the addition of N-acetyl-l-cysteine (NAC), indicating that free radical-induced DNA damage was mediated by reactive oxygen species. (ox.ac.uk)
  • Damage can often be repaired (DNA REPAIR). (lookformedical.com)
  • The protein regulates cell growth and division by monitoring DNA damage. (medlineplus.gov)
  • The altered protein cannot regulate cell proliferation effectively and allows DNA damage to accumulate in cells. (medlineplus.gov)
  • Bleomycin's primary mechanism of action is to oxidatively damage DNA by binding to metal ions, including iron, and forming metallic bleomycin complexes. (yashodahospitals.com)
Mice2
  • We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
  • ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
MRNA3
  • A majority of histologically normal fields surrounding the neoplastic growths also show reduced MBD4 mRNA expression (a field defect) compared to histologically normal tissue from individuals who never had a colonic neoplasm. (wikipedia.org)
  • In human breast cancer cells stably transfected with SIAH-1, Kid/KIF22 protein level was markedly reduced whereas, the Kid/KIF22 mRNA level was increased. (hal.science)
  • High expression of GRB2 associated binding protein 3 mRNA predicts positive prognosis in melanoma. (cdc.gov)
Enzyme3
  • Also, a reduction in hPaf1 level resulted in reduced MLL1 expression and a corresponding decrease in the level of CHD1 (Chromohelicase DNA-binding protein 1), an ATPase dependent chromatin remodeling enzyme that specifically binds to H3K4 di and trimethyl marks. (nebraska.edu)
  • Bioactivation of pyrrolizidine alkaloids by the P450 enzyme system produces highly reactive toxic metabolites, which can bind to proteins or DNA, forming adducts. (usda.gov)
  • It is believed that Bleomycin chelates metal ions to produce a pseudo-enzyme that reacts with the oxygen-producing superoxide and hydroxyl free radicals that cleave DNA. (yashodahospitals.com)
Cells15
  • Protein expression and function of organic anion transporters in short-term and long-term cultures of Huh7 human hepatoma cells. (cancerindex.org)
  • Changes in the redox state of cells affect proteins, lipids, and nucleic acids in different ways. (hindawi.com)
  • Treatment of Ishikawa cells with the AhR agonist [3H]2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) gave a radiolabeled nuclear complex that sedimented at 6.0 S in sucrose density gradients, and Western blot analysis confirmed that Ishikawa cells expressed human AhR and AhR nuclear translocator (Arnt) proteins. (tamu.edu)
  • The actin-binding protein Girdin is a hub protein that interacts with multiple proteins to regulate motility and Akt and trimeric G protein signaling in cancer cells. (fujita-hu.ac.jp)
  • To test this model the induction of the HIF-1alpha subunit and the HIF target gene, glucose-transporter-1, was examined in a variety of mutant cells that lacked mitochondrial DNA (rho0) or had other genetic defects in mitochondrial respiration. (ox.ac.uk)
  • Because many T cell-recognized tumor antigens are nonmutated self-proteins, tolerance mechanisms are likely to render high avidity T cells unresponsive or cause T cell elimination by clonal deletion. (birmingham.ac.uk)
  • DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells. (broadinstitute.org)
  • Limited DNA end resection is the key to impaired homologous recombination in BRCA1-mutant cancer cells. (broadinstitute.org)
  • The loss of DYNLL1 enables DNA end resection and restores homologous recombination in BRCA1-mutant cells, thereby inducing resistance to platinum drugs and inhibitors of poly(ADP-ribose) polymerase. (broadinstitute.org)
  • In cells, DYNLL1 limits nucleolytic degradation of DNA ends by associating with the DNA end-resection machinery (MRN complex, BLM helicase and DNA2 endonuclease). (broadinstitute.org)
  • It was observed that SIAH-1 protein is widely expressed in different normal tissues, and in cells lines but showing some differences in western blotting profiles. (hal.science)
  • The TP53 gene provides instructions for making a protein, called p53, that is located in the nucleus of cells throughout the body, where it attaches (binds) directly to DNA. (medlineplus.gov)
  • When these proteins are turned on (activated) by binding to other molecules, signaling pathways are triggered within cells that promote cell proliferation. (medlineplus.gov)
  • Bleomycin inhibits the uncontrolled cell division in cancer-causing cells by inhibiting DNA synthesis. (yashodahospitals.com)
  • Bleomycin interacts with cancerous cells and binds with the DNA, and inhibits cell synthesis by breaking DNA strands. (yashodahospitals.com)
Carcinoma1
  • DNA adduct formation forms the basis of the carcinogenesis theory for pyrrolizidine alkaloids and why their consumption is associated with multiple types of neoplasia, including hemangiosarcoma, hepatocellular adenoma/carcinoma, and bronchoalveolar adenoma/carcinoma in various rodent models. (usda.gov)
Transcription5
  • The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. (cancerindex.org)
  • Rel frequently combines with other related proteins (NF-KAPPA B, I-kappa B, relA) to form heterodimers that regulate transcription. (childrensmercy.org)
  • GATA binding protein 3 (GATA3) is a zinc-finger pioneer transcription factor involved in diverse processes. (bmj.com)
  • Although menin interacts with a variety of nuclear proteins including transcription factors, it is unknown whether menin itself can directly bind DNA. (princeton.edu)
  • Strong lymphoid nuclear expression of SOX11 transcription factor defines lymphoblastic neoplasms, mantle cell lymphoma and Burkitt's lymphoma. (nordiqc.org)
Interacts1
  • Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. (cancerindex.org)
Malignant2
  • Bleomycin is therapeutically used to treat malignant neoplasms such as sarcomas, lymphomas, melanomas, and germinal cell cancers. (yashodahospitals.com)
  • Bleomycin is approved for use in adults and can be used alone or with other drugs as palliative treatment and management of malignant neoplasms. (yashodahospitals.com)
Gene Expression2
  • GATA3 regulates gene expression through binding nucleosomal DNA and facilitating chromatin remodelling. (bmj.com)
  • Our observations also underline the need to re-evaluate the results of gene expression obtained by qRT-PCR and relate it to the protein expression and cellular localization when matched normal and tumoral tissues are analyzed. (hal.science)
Double-stranded1
Nuclear4
  • Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 (this gene) comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). (wikipedia.org)
  • The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. (cancerindex.org)
  • Furthermore, detailed mutagenesis studies indicate that positively charged residues in two nuclear localization signals mediate direct DNA binding as well as repression of cell proliferation. (princeton.edu)
  • Nuclear expression of sox11 is highly associated with mantle cell lymphoma but is independent of t(11;14)(q13;q32) in non-mantle cell B-cell neoplasms. (nordiqc.org)
Regulate1
Breast1
  • The Smad7 protein is negative regulator of the TGF-β signaling pathway, which is upregulated in patients with breast cancer. (cancerindex.org)
Mismatch repair3
  • Further, MBD4 gene mutations are detected in tumors with primary microsatellite instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. (wikipedia.org)
  • MBD4 has a special relationship with DNA mismatch repair (MMR). (wikipedia.org)
  • It is a form of genome instability associated with defects in DNA MISMATCH REPAIR. (lookformedical.com)
Cancers1
  • High mobility group B (HMGB) proteins have been previously related to the onset and progressions of cancers of different origins. (hindawi.com)
Mutations5
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Upon DNA replication, these mismatches cause C to T transition mutations. (wikipedia.org)
  • G:U and G:T mismatches, upon DNA replication, give rise to C to T transition mutations. (wikipedia.org)
  • The COOH terminus of menin mediates binding to DNA, but MEN1 disease-derived mutations in the COOH terminus abolish the ability of menin to bind DNA. (princeton.edu)
  • TP53 gene mutations result in the production of an altered p53 protein that cannot bind to DNA. (medlineplus.gov)
Chemotherapy1
  • Therefore, we infer that DYNLL1 is an important anti-resection factor that influences genomic stability and responses to DNA-damaging chemotherapy. (broadinstitute.org)
Ubiquitin1
  • SIAH proteins are the human members of an highly conserved family of E3 ubiquitin ligases. (hal.science)