• Single-stranded DNA is produced during all aspects of DNA metabolism: replication, recombination, and repair. (wikipedia.org)
  • SSB protein domains in bacteria are important in its function of maintaining DNA metabolism, more specifically DNA replication, repair, and recombination. (wikipedia.org)
  • DSBs usually act as transcriptional factors to regulate the genes' expressions, while SSBs usually play roles in DNA replication, recombination, and repair, etc. (biomedcentral.com)
  • SSB binds with ssDNA with high affinity and low specificity, and is mainly involved in DNA replication, recombination and repair. (biomedcentral.com)
  • Biophysical studies showed that the orthologs of the two OB fold/one zinc finger replication protein A in Hethanosarcina acetivorans and Methanopyrus kandleri exhibit two binding modes, wrapping and stretching of DNA. (johnshopkins.edu)
  • In contrast to the RNA-directed DNA synthesis by telomerase, ALT relies on recombination and replication of telomere DNA to extend telomeres. (massgeneral.org)
  • The processes that maintain telomeres employ replication protein A (RPA), a single-stranded DNA binding protein. (massgeneral.org)
  • Dr. Zou and his team had previously investigated the role for RPA at telomeres3 and found that it associated transiently with telomeres during S phase of DNA replication. (massgeneral.org)
  • And indeed the team found that in cells that use the ALT pathway, RPA binds persistently to telomeres, not detaching after replication. (massgeneral.org)
  • DNA is the biological information that instructs cells how to exist in an ordered fashion: accurate replication is thus one of the most important events in the life cycle of a cell. (embl.de)
  • Pur-alpha is a highly conserved, sequence-specific DNA- and RNA-binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. (embl-heidelberg.de)
  • The human Pur factor binds strongly to a sequence element repeated withinzones of initiation of DNA replication in several eukaryotic cells. (embl-heidelberg.de)
  • We are studying how genome integrity is preserved and the consequences of modifying the program of DNA replication. (umu.se)
  • Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. (nih.gov)
  • 2004. Replication protein A and the Mre11 center dot Rad50 center dot Nbs1 complex co-localize and interact at sites of stalled replication forks. (nih.gov)
  • It has a small (4500 nucleotides) genome that encoding four DNA helicases that are required for viral DNA replication and efficient packaging of single stranded DNA into virions. (utoledo.edu)
  • Rep78 and Rep68 also have a site specific nuclease activity, residing in an N-terminal structure, that creates a priming site in the template for DNA replication. (utoledo.edu)
  • The smaller replication proteins, Rep40 and Rep52, require ATP to bind DNA, show no sequence specificity in DNA binding and lack the N-terminal nuclease domains. (utoledo.edu)
  • A shortage of this protein may prevent normal DNA replication and repair, causing widespread damage to a person's genetic information over time. (medlineplus.gov)
  • HotStarTaq DNA polymerase (Qiagen). (cdc.gov)
  • DNA Polymerase I, Large (Klenow) Fragment is available at Gentaur for Next week delivery. (telospub.com)
  • A proteolytic product of E. coli DNA Polymerase I Biomolecule/Target: Alternates names: DNA Polymerase I,DNA Polymerase I, Large Fragment,Klenow Fragment,DNA. (telospub.com)
  • ssDNA template is hybridized to a sequencing primer and incubated with the enzymes DNA polymerase , ATP sulfurylase , luciferase and apyrase , and with the substrates adenosine 5´ phosphosulfate (APS) and luciferin . (wikidoc.org)
  • DNA polymerase incorporates the correct, complementary dNTPs onto the template. (wikidoc.org)
  • A number of DNA polymerases have been grouped under the designation of DNA polymerase family B. Six regions of similarity (numbered from I to VI) are found in all or a subset of the B family polymerases. (embl.de)
  • Crystal structures of an NH2-terminal fragment of T4 DNA polymerase and its complexes with single-stranded DNA and with divalent metal ions. (embl.de)
  • We report the crystal structure of an NH2-terminal 388-residue fragment of T4 DNA polymerase (protein N388) refined at 2.2 A resolution. (embl.de)
  • abstract = "The architecture of single-stranded DNA-binding proteins, which play key roles in DNA metabolism, is based on different combinations of the oligonucleotide/oligosaccharide binding (OB) fold. (johnshopkins.edu)
  • Our pAgo candidate nicks and cuts plasmid DNA nonspecifically in the absence of guide in in vitro assays, presumably due to the presence of single-stranded DNA that co-purifies with the protein," the abstract continued. (genengnews.com)
  • We created several deletion mutants of this protein based on its conserved motifs, and from these structures functional chimeras were synthesized, supporting the hypothesis that gene duplication and recombination could lead to novel functional forms of single-stranded DNA-binding proteins. (johnshopkins.edu)
  • Dr. Zou and his team postulated that such cancer cell lines were those without active telomerase, relying instead on the ALT pathway, which lengthens telomeres through recombination with telomeric DNA sequences from the same or other chromosomes. (massgeneral.org)
  • RPA functions as a DNA repair protein that promotes DNA recombination, and the ALT pathway is known to be a process that is dependent on recombination. (massgeneral.org)
  • In the presence of homologous donor DNA and pAgo," they emphasized, "homologous recombination is increased by up to 50% at targeted gene loci, suggesting that gene editing applications are feasible. (genengnews.com)
  • In human cells, there exist two canonical pathways of DSB repair: Non-Homologous End Joining (NHEJ) and Homologous Recombination (HR). Two protein complexes that rapidly bind DNA ends coordinate these separate pathways: the Ku70-Ku80 heterodimer (Ku) and the Mre11-Rad50-Nbs1 complex (MRN), respectively. (utexas.edu)
  • Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. (lu.se)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Telomeric DNA and shelterin proteins prevent loss of essential genetic information during cell division. (cdc.gov)
  • The telomeric repeat-binding factor 1 (Trf1) and 2 (Trf2), and the protection of telomere 1 (Pot1) are involved in telomere maintenance by preventing telomere end-to-end fusion through proper folding of the telomere. (cdc.gov)
  • Double- and single-stranded telomere DNA-binding proteins Trf1/Trf2 and Pot1, as well as telomeric DNA damage foci LambdaH2AX and 53BP1, were influenced at 12 wk and 24 wk by both diet and exposure. (cdc.gov)
  • Since telomerase is recruited to and elongates the single-stranded telomeric 3 ′ overhangs, the generation of 3 ′ overhangs is an essential step in the maintenance process. (lu.se)
  • Single-strand DNA-binding protein (SSB) is a protein found in Escherichia coli (E. coli) bacteria, that binds to single-stranded regions of deoxyribonucleic acid (DNA). (wikipedia.org)
  • Bound or free: interaction of the C-terminal domain of E. coli single-stranded DNA binding protein (SSB) with the tetrameric core of SSB. (cipps.org.au)
  • Single Stranded DNA Binding Protein, E. coli is available at Gentaur for Next week delivery. (telospub.com)
  • In vivo, we are able to program our pAgo candidate with 5′-phosphorylated DNA guides to cleave both plasmid and chromosomal targets to reduce survival under selective conditions in E. coli . (genengnews.com)
  • Inthe absence of DNA, glutathione S-transferase-Pur alpha binds to p56RB, anNH2-terminal-truncated Rb protein purified from Escherichia coli,containing the T-antigen binding domain, to form multimeric complexes. (embl-heidelberg.de)
  • In this work, we used a hybrid instrument that combines single-molecule fluorescence and force spectroscopy to directly visualize the movement of Escherichia coli SSB on long polymeric ssDNA. (wustl.edu)
  • The apparent diffusion coefficient of E. coli SSB thus determined ranged from 70,000 to 170,000 nt 2 /s, which is at least 600 times higher than that determined from SSB diffusion on short ssDNA oligomers, and is within the range of values reported for protein diffusion on double-stranded DNA. (wustl.edu)
  • The RAD52-like protein ODB1 is required for the efficient excision of two mitochondrial introns spliced via first-step hydrolysis. (uni-halle.de)
  • In the majority of cases, Rf genes produce proteins that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events. (frontiersin.org)
  • These unusual ORFs are maternally inherited, and effectively translated into novel mitochondrial proteins, with the resulting failure to produce functional pollen as the sole observed phenotype ( Chen and Liu, 2014 ). (frontiersin.org)
  • In the majority of cases, Rf genes produce proteins that bind specifically to the CMS conferring transcripts in the mitochondria and promote processing events leading to a strong reduction in the production of mitochondrial CMS-inducing proteins (reviewed in Chen and Liu, 2014 ). (frontiersin.org)
  • In some genomic regions, the lack of protospacer adjacent motifs (PAMs) means that CRISPR is bound to lose its grip. (genengnews.com)
  • We present a systematic analysis of sequence motifs found in metazoan protein factors involved in constitutive pre-mRNA splicing and in alternative splicing regulation. (embl.de)
  • Most unexpectedly, genes coding for similar proteins were found in the genomes of eukaryotes, including humans. (johnshopkins.edu)
  • Plasmid stabilization/stability protein-encoding genes were observed in some plasmids but were not established for participating in plasmid segregation. (frontiersin.org)
  • The goal of this study was to describe the regulation of expression of these genes and proteins along with their relationship to telomere length in an animal model comparing different diets and a simulated occupational exposure. (cdc.gov)
  • Genes are arranged linearly along the DNA of chromosomes. (msdmanuals.com)
  • In this study we investigate the feasibility of applying the compatible solute, osmolyte and radiation protector Ectoine (THP(B)), as a scavenger molecule during SAXS measurements of the single-stranded DNA-binding protein Gene-V Protein (G5P/GVP). (rsc.org)
  • The genetic material is fundamentally the double helix of the DNA molecule. (whale.to)
  • Any substance that damages DNA or chromosomes, or changes genetic activity, is genotoxic because it is acting on the same material, i.e. the DNA molecule. (whale.to)
  • Single-molecule studies reveal how the DNA-repair protein RecA overcomes competition from another protein to bind to single-stranded DNA, and how other mediator proteins assist in this process. (nature.com)
  • It has long been a mystery how a bacterial cell is able to import a molecule as complex as DNA. (mpg.de)
  • The stochastic nature of single-molecule charge transport measurements requires collection of large data sets to capture the full complexity of a molecular system. (imperial.ac.uk)
  • Here we present such an approach based on multivariate pattern analysis and apply it to simulated and experimental single-molecule charge transport data. (imperial.ac.uk)
  • Here, I use a combination of ensemble biochemical assays as well as high-throughput single-molecule microscopy to visualize the repair process. (utexas.edu)
  • of the DNA molecule. (medlineplus.gov)
  • A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
  • A gene is a segment of DNA that provides the code to construct a protein or RNA molecule. (msdmanuals.com)
  • While DSBs involve in binding to particular dsDNA sequences, to modulate the process of transcription, to cleave DNA molecules, or to be involved in chromosome packaging and transcription in the cell nucleus, etc. (biomedcentral.com)
  • Using profile analysis we constructed a database enriched in protein sequences containing one or more presumptive copies of the RNA-recognition motif (RRM). (embl.de)
  • We present a comprehensive table of 125 sequences containing 252 RRMs, including 22 previously unreported RRMs in 17 proteins. (embl.de)
  • 2 alleles may have slight differences or the same DNA sequences. (msdmanuals.com)
  • However, our mesophilic protein also contains a putative single-stranded DNA binding or repA domain. (genengnews.com)
  • Many eukaryotic proteins that are known or supposed to bind single-stranded RNA contain one or more copies of a putative RNA-binding domain of about 90 amino acids. (embl.de)
  • This is known as the eukaryotic putative RNA-binding region RNP-1 signature or RNA recognition motif (RRM). (embl.de)
  • The presence of a putative RRM in these proteins, which are implicated in a variety of cellular processes, strongly suggests that their function involves binding to RNA. (embl.de)
  • The winged helix domains are at opposite ends of the extended coiled coil with two putative DNA-recognition helices separated by 55A and rotated by 83 degrees. (rcsb.org)
  • CusabioAlternative Name(s): DsDNA-binding protein A (rpbB)Gene Names: dsbAResearch Areas: Cell. (joplink.net)
  • A new technology based on the protein Argonaute from Natronobacterium gregoryi (NgAgo) could change how gene editing is approached. (genengnews.com)
  • The retinoblastoma protein, Rb, is detected in extracts of monkey CV-1cells complexed with Pur alpha, a sequence-specific single-strandedDNA-binding protein implicated in control of gene transcription and DNAreplication. (embl-heidelberg.de)
  • Sequence of cDNA comprising the human pur gene and sequence-specificsingle-stranded-DNA-binding properties of the encoded protein. (embl-heidelberg.de)
  • DNA methylation is a common epigenetic marker that regulates gene expression. (biomedcentral.com)
  • The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. (nature.com)
  • Western blotting and quantitative real-time reverse transcription PCR (qRT-PCR) were used to determine protein and gene levels. (bvsalud.org)
  • This gene provides instructions for making one member of a protein family called RecQ helicases. (medlineplus.gov)
  • Mutations in the RECQL4 gene prevent cells from producing any RECQL4 protein or change the way the protein is pieced together, which disrupts its usual function. (medlineplus.gov)
  • One of the few antigens against which mammals develop protective immunity is the highly polymorphic OspC protein, encoded by the ospC gene on the cp26 plasmid. (cdc.gov)
  • By means of DNA sequencing of a large sample collection of the pathogen from across the United States, we studied the gene for the bacterium's highly diverse OspC protein, protective immunity against which develops in animals. (cdc.gov)
  • Decreased Trf1-Trf2 negatively regulates telomere length and DNA damage foci in rat liver tissue after a high-fat diet and welding fume exposure. (cdc.gov)
  • Telomere attrition resulting in DNA damage contributes to liver pathology. (cdc.gov)
  • At 12 and 24 wk, the effect on shelterin proteins and telomere length was examined in peripheral blood mononuclear cells (PBMCs) and homogenized liver tissue. (cdc.gov)
  • In conclusion, our data suggest that disruption/down-regulation of single-stranded Pot1 and double-stranded Trf1/Trf2 expression in liver might act as an anti-apoptotic mechanism in the DNA-damage response leading to multistep liver injury by involvement in the telomere response to diet changes and WF exposure. (cdc.gov)
  • We therefore focus on elucidating the roles of the telomere binding proteins in this process. (lu.se)
  • In contrast, MRN along with the long-range nuclease Exo1 and helicase BLM digests the DNA to create long 3' single-stranded DNA overhangs, which are rapidly bound by the single-stranded DNA binding protein RPA. (utexas.edu)
  • Human Pur alpha, expressed as a glutathioneS-transferase fusion protein, specifically binds to the hypophosphorylatedform of Rb with an affinity as high as that of SV40 large T-antigen. (embl-heidelberg.de)
  • Early studies showed that FinO specifically binds the 3' transcriptional terminator structure of FinP in a manner that critically relies on both the GC-rich hairpin and 3' polypyrimidine tail that define the terminator 16 , and further work showed that the base of the hairpin and 3' tail are both strongly protected from ribonuclease degradation by FinO 17 . (nature.com)
  • The great progress of the structure genomics project [ 9 ] results that more and more high resolution 3D structures for DSBs and SSBs are available now, which makes it possible to investigate the common structural differences between SSB and DSB that are responsible for the binding specificity. (biomedcentral.com)
  • In the meantime, the investigation results can help to annotate or refine the annotation of the proteins with known structures yet unknown or not fully understood functions. (biomedcentral.com)
  • But, of course, we also want to identify target structures that could inhibit DNA transfer and, for example, stem the spread of antibiotic resistance," D'Imprima says. (mpg.de)
  • The larger Rep proteins, Rep78 and Rep68, assemble into stable, hexameric oligomers on specific secondary structures on the 3' and 5' termini of the single stranded viral DNA. (utoledo.edu)
  • Our studies are aimed at understanding how these proteins assemble on DNA structures and the specificity of their interaction with DNA. (utoledo.edu)
  • The telomeres are the DNA-protein structures at the ends of eukaryotic chromosomes, which serve to protect and stabilize the genome. (lu.se)
  • The structures of many DNA quadru- magnetization ( 11 - 13 ). (lu.se)
  • We report here the cloning and sequencing of a cDNA encodinga protein with strong affinity for the PUR element. (embl-heidelberg.de)
  • Studies of the thermodynamics of binding of ligands to glycyl tRNAs suggest that the formation of glycyl adenylate, an obligate intermediate on the reaction pathway, is accompanied by a significant conformation change in the protein that alters the affinity of the enzyme for tRNA. (utoledo.edu)
  • A protein interacting with an A-T-rich region that is a positive control element within the SP6 κ promoter was purified and identified as CArG-box binding factor-A. The purified protein was shown to interact specifically with the coding strand of single-stranded DNA and, with lower affinity, with double-stranded DNA. (lu.se)
  • Chromosomes consist of two interwound DNA strands millions of base pairs in length. (nature.com)
  • DNA (deoxyribonucleic acid) is the cell's genetic material, contained in chromosomes within the cell nucleus and mitochondria. (msdmanuals.com)
  • Whereas the polypeptide serving this function in bacteria contains one OB fold, the eukaryotic functional homolog comprises a complex of three proteins, each harboring at least one OB fold. (johnshopkins.edu)
  • Understanding the binding specificity of a DNA binding protein is helpful for the research of protein functions. (biomedcentral.com)
  • Though there are some researches [ 5 - 7 ] on the SSB and DSB respectively, few attentions have been paid on investigating what makes SSB and DSB have such different kind of binding specificity. (biomedcentral.com)
  • In the crown we may have discovered a crucial switch for the recognition and binding of DNA," says Edoardo D'Imprima of the Department of Structural Biology at the Max Planck Institute in Frankfurt. (mpg.de)
  • Thesingle-stranded DNA Pur alpha recognition element disrupts thesecomplexes. (embl-heidelberg.de)
  • The RNA recognition motif protein CP33A is a global ligand of chloroplast mRNAs and is essential for plastid biogenesis and plant development. (uni-halle.de)
  • Here, we investigate the structural basis for RNA recognition by ProQ/FinO proteins, through the crystal structure of the ProQ/FinO domain of the Legionella pneumophila DNA uptake regulator, RocC, bound to the transcriptional terminator of its primary partner, the sRNA RocR. (nature.com)
  • SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. (cdc.gov)
  • This can make the process of genome assembly more difficult, particularly for sequence containing a large amount of repetitive DNA . (wikidoc.org)
  • A robust and cost-effective way for measuring whole genome methylation is Methyl-CpG binding domain-based capture followed by seque. (biomedcentral.com)
  • Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle developed by Mostafa Ronaghi and Pål Nyrén (published in Analytical Biochemistry 1996 and Science 1998). (wikidoc.org)
  • Small RNA molecules are generally used as primers for chain elongation, although terminal proteins may also be used for the de novo synthesis of a DNA chain. (embl.de)
  • Aminoacyl tRNA synthetases are a class of enzymes that ensure the fidelity of protein synthesis by attaching amino acids to their cognate tRNAs. (utoledo.edu)
  • Protein synthesis, folding, and tertiary and quaternary structure ultimately determine much of the body's structure and function. (msdmanuals.com)
  • Glass or quartz nanopipettes have found increasing use as tools for studying the biophysical properties of DNA and proteins, and as sensor devices. (imperial.ac.uk)
  • 2014. Protein-Protein Interactions of the Yak1 Kinase . (northcentralcollege.edu)
  • ATM kinase phosphorylation and DNA damage activation was observed in the liver at 12 wk of WF exposure. (cdc.gov)
  • Ku encircles the DNA ends and recruits other factors, such the kinase DNA-PKcs, to bluntly ligate the ends back together. (utexas.edu)
  • Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. (lu.se)
  • The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the KU p70/p86 dimer (g22p1/g22p2) and PRKDC. (lu.se)
  • Extracellular vesicle DNA from human melanoma tissues contains cancer-specific mutations. (gu.se)
  • Results Protein-altering de novo mutations in PURA were identified in four subjects. (bmj.com)
  • However, mutations in the gun-like structure cause the crown to disintegrate, so that the bacterial cell is no longer able to absorb DNA. (mpg.de)
  • Mutations are detected by lack of binding to wild-type probes and/or by binding to probes specific for commonly occurring mutations. (cdc.gov)
  • Because molecular beacons can use different fluorophores, real-time PCR assays can be designed in which different DNA fragments or mutations can be amplified and detected simultaneously in the same tube. (cdc.gov)
  • For example, a single-well assay has been developed that uses five molecular beacons to detect mutations associated with rifampin resistance in M. tuberculosis bacteria and appears to perform similarly as the line-probe assays. (cdc.gov)
  • The eponymous FinO protein was discovered as a regulator of F plasmid conjugation nearly 50 years ago, and acts to bind a single partner sRNA called FinP to stabilize FinP and facilitate its interactions with its antisense partner, the mRNA encoding the major F plasmid transcription factor, TraJ 5 . (nature.com)
  • Another plasmid-encoded ProQ/FinO family member, FopA, has also been shown to interact with a single antisense RNA 11 . (nature.com)
  • Example of a pyrogram showing the nucleotide sequence in a specific section of DNA. (wikidoc.org)
  • Currently, a limitation of the method is that the lengths of individual reads of DNA sequence are in the neighborhood of 300-500 nucleotides, shorter than the 800-1000 obtainable with chain termination methods (e.g. (wikidoc.org)
  • The structure of the 3'-5' exonuclease domain of protein N388 closely resembles the corresponding region in the Klenow fragment despite minimal sequence identity. (embl.de)
  • The search process is remarkable in two respects: the homologous partner can be found even among the many millions of non-homologous segments, and the crucial base sequence can be recognized even when it is largely buried within a DNA double helix. (nature.com)
  • The PPR protein family is consequently divided in subfamilies depending on the number and type of repeats present in their sequence as well as optional C-terminal domains. (frontiersin.org)
  • These protein either stabilize (HuR) or destabilize (AUF1, tristetraprolin, and KSRP) cytokine mRNAs by binding to particular sequence elements, frequently AU-rich components (ARE) situated in the 3 untranslated area (3-UTR) from the mRNA. (iassist2012.org)
  • The need for RBPs like AUF1, tristetraprolin (TTP), or KSRP (KH-type splicing regulatory proteins, also called KHSRP or significantly upstream sequence-binding proteins 2 (FuBP2)) for immune system regulatory processes continues to be confirmed in knockout pets [5C7]. (iassist2012.org)
  • KSRP continues to be described to modify c-myc transcription by binding towards the significantly upstream sequence from Prinaberel the myc promoter [8] and to regulate TNF-promoter activity [9]. (iassist2012.org)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing POLBc domain in the selected taxonomic class. (embl.de)
  • To display all proteins with a RRM domain in a specific node, click on it. (embl.de)
  • These long molecules inevitably suffer breakage, which can be induced by ionizing radiation, biochemicals or natural DNA processing within cells. (nature.com)
  • This protein family is largely expanded in land plant genomes. (frontiersin.org)
  • There are two kinds of DNAs, single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). (biomedcentral.com)
  • The Crystal Structure of the Herpes Simplex Virus 1 Ssdna-Binding Protein Suggests the Structural Basis For Flexible, Cooperative Single-Stranded Dna Binding. (atomistry.com)
  • SSB can also locally diffuse on ssDNA, which allows it to quickly reposition itself while remaining bound to ssDNA. (wustl.edu)
  • A mutation that inhibited binding of the protein to the A-T-rich region also aborted the transcriptional stimulatory effect of the region. (lu.se)
  • Latest platform of Pyrosequencing (GS FLX from 454 Life Sciences which is owned by Roche) can generate 100 million nucleotide data in a 7 hour run with a single machine. (wikidoc.org)
  • The proteins binds to AREs in the 3-UTR of mRNAs and recruits enzymes mixed up in 5- and 3-mRNA decay [10]. (iassist2012.org)
  • DNA/RNA-binding repeats in PUR-alpha/beta/gamma and in hypothetical proteins from spirochetes and the Bacteroides-Cytophaga-Flexibacter bacteria. (embl-heidelberg.de)
  • Theprotein binds preferentially to the purine-rich single strand of thiselement, PUR. (embl-heidelberg.de)
  • The most deleterious DNA damages are double-strand breaks (DSBs), and a single unrepaired DSB can lead to cell death. (utexas.edu)
  • The ataxia telangiectasia Rad3-related (ATR) protein responds to UV damage, whereas the ataxia telangiectasia mutated (ATM) protein responds to double-strand breaks (DSBs) caused by ionizing radiation and radiomimetic compounds. (medscape.com)
  • Association of human Pur alpha with the retinoblastoma protein, Rb,regulates binding to the single-stranded DNA Pur alpha recognitionelement. (embl-heidelberg.de)
  • The tops represent light emission and nucleotide binding. (wikidoc.org)
  • This function is performed by DNA- directed DNA-polymerases ( EC 2.7.7.7 ) by adding nucleotide triphosphate (dNTP) residues to the 5'-end of the growing chain of DNA, using a complementary DNA chain as a template. (embl.de)
  • Molecular beacons can discriminate between targets differing by a single nucleotide. (cdc.gov)
  • Rep52 and Rep40 are implicated in packaging plus and minus single stranded DNA into virions. (utoledo.edu)
  • As well as stabilizing this single-stranded DNA, SSB proteins bind to and modulate the function of numerous proteins involved in all of these processes. (wikipedia.org)
  • Domain analysis of this protein, which contains a single OB fold, showed that its architecture is similar to the functional homologs thought to be unique to the Crenarckaeotes. (johnshopkins.edu)
  • Pur-alpha can influence viral interaction through functional associations, for example with the Tat protein and TAR RNA of HIV-1, and with large T-antigen and DNA regulatory regions of JC virus. (embl-heidelberg.de)
  • For example, the (SSB)65 binding mode, in which approximately 65 nucleotides of DNA wrap around the SSB tetramer and contact all four of its subunits, is favoured at high salt concentrations in vitro. (wikipedia.org)
  • At lower salt concentrations, the (SSB)35 binding mode, in which about 35 nucleotides bind to only two of the SSB subunits, tends to form. (wikipedia.org)
  • PURA encodes Pur-α, a highly conserved multifunctional protein that has an important role in normal postnatal brain development in animal models. (bmj.com)
  • KSRP is certainly a multifunctional, single-stranded nucleic acidity- Prinaberel (DNA- or RNA-) binding proteins. (iassist2012.org)
  • The RECQL4 protein helps stabilize genetic information in the body's cells and plays a role in replicating and repairing DNA. (medlineplus.gov)
  • Unreported homologies in the RRM-enriched database to the metazoan SR family of splicing factors are described for an Arg-rich human nuclear protein and two yeast proteins (S. pombe mei2 and S. cerevisiae Npl3). (embl.de)
  • The 95 amino acid residue protein contains a winged helix DNA-binding domain with an extended C-terminal alpha-helix that leads to dimerization by forming a two-stranded, antiparallel coiled-coil rod. (rcsb.org)
  • PPR proteins have in common a canonical P-type 35 amino acid domain repeated in tandem up to 30 times. (frontiersin.org)
  • The RRM structure consists of four strands and two helices arranged in an alpha/beta sandwich, with a third helix present during RNA binding in some cases. (embl.de)
  • 2007. Pyrosequencing AB was started to commercialize the machine and reagent for sequencing of short stretches of DNA. (wikidoc.org)
  • Protein-DNA interactions are essential for many biological processes. (biomedcentral.com)
  • Possible DNA-binding interactions are discussed on the basis of comparisons to other winged helix proteins. (rcsb.org)
  • However, the most common form is a protein with two OB folds and one zinc finger domain. (johnshopkins.edu)
  • Taxonomic distribution of proteins containing POLBc domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with POLBc domain is also avaliable . (embl.de)
  • The R3H motif: a domain that binds single-stranded nucleic acids. (embl-heidelberg.de)
  • Likewise, the ProQ/FinO domain-containing protein RocC of Legionella pneumophila interacts with only one trans -acting sRNA (RocR) to repress post-transcriptionally multiple mRNA targets 6 . (nature.com)
  • Similarly, a minimal ProQ/FinO domain protein, NMB1681, has been shown to bind a range of structured RNAs in Neisseria meningitidis 15 . (nature.com)
  • In this study we investigated the effect of LXR agonist T0901317 (T317) on HCC development and its relationship with RalA binding protein 1 (RALBP1)-associated EPS domain containing 2 (REPS2)/epidermal growth factor receptor (EGFR) signaling axis. (bvsalud.org)
  • The motif also appears in a few single stranded DNA binding proteins. (embl.de)
  • Binding of Pur alpha top56RB, the COOH-terminal portion of Rb, is inhibited by a syntheticpeptide containing the T-antigen Rb-binding motif. (embl-heidelberg.de)
  • Structural analysis and RNA binding studies reveal that other ProQ/FinO domains also recognize related transcriptional terminators with different specificities for the length of the 3' ssRNA tail. (nature.com)
  • PPR proteins represent the most frequent protein class among identified Rfs and they exhibit ideal characteristics to evolve into restorer of fertility when the mechanism of restoration implies a post-transcriptional action. (frontiersin.org)
  • Whereas transcription elements and epigenetic systems are in Prinaberel charge of transcriptional control, posttranscriptional legislation (splicing, mRNA editing, balance, translatability, and localization) frequently depends upon RNA-binding protein (RBP) [4]. (iassist2012.org)
  • Using promoter activity assay and chromatin immunoprecipitation (CHIP) assay we demonstrated that T317 enhanced REPS2 expression at the transcriptional level via promoting the binding of LXR protein to the LXR-response element (LXRE) in the REPS2 promoter region. (bvsalud.org)
  • Researchers at the Max Planck Institute of Biophysics and Goethe University in Frankfurt have now gained new insights into exactly how bacteria import DNA. (mpg.de)
  • Extrachromosomal DNA provides great impact in the evolution of bacteria in adapting to their surroundings. (frontiersin.org)
  • Plasmids are one of the primary sources for extrachromosomal DNA in bacteria and are capable of self-transmission. (frontiersin.org)
  • According to the researchers, this finding suggests that lethality is due to unrepaired double-stranded DNA breaks. (genengnews.com)
  • NgAgo may be programmed with guide DNA (red) to cut DNA (purple) at specific regions, enabling precise genetic modifications. (genengnews.com)
  • Genetic studies have shown that the crown is not formed by the secretin protein itself. (mpg.de)
  • 2004. Genetic alterations and DNA repair in human carcinogenesis. (nih.gov)
  • The purine-rich element binding (Pur) protein family protein consists PURalpha/beta/gamma in humans. (embl-heidelberg.de)