Dna are recombinant adenovirus. Medical search. Frequent questions

E1-deleted (including E1a-deleted) adenoviruses are replication defective and are commonly used as viral vectors to carry therapeutic genes for gene therapy. (hindawi.com)
Recombinant human adenoviral vectors provide an efficient delivery system and have many advantages over other viral and non-viral delivery vehicles. (illinois.edu)
The possibility to replace e.g. the E3 region with foreign DNA makes adenoviruses also suitable as cloning vectors for proteins of other respiratory viruses. (eur.nl)
This review summarizes different DNA vector modifications to enhance biological efficacy and efficiency of therapeutical vectors, aiming for low toxicity, high specificity, and biological efficacy-the cornerstones for successful translation of gene therapy into the clinic. (genengnews.com)
2 , 3 Viral gene transfer strategies [retroviruses, 4 adenoviruses, 5 adeno-associated viruses, 6 , 7 herpes viruses, 8 lentiviruses, 9 and Epstein-Barr viruses (EBV) 10 ] show by far the highest transfection efficiencies, whereas nonviral vectors are limited in their efficacy to deliver genes. (genengnews.com)
12 , 13 Nevertheless, independently of used gene delivery strategy, optimized and improved DNA vectors are essential and will boost the promising field of gene therapy further to the clinics. (genengnews.com)
This review summarizes different DNA vector modifications to enhance biological efficacy and efficiency of therapeutical vectors ( Figures 1 and 2 ). (genengnews.com)
US officials immediately began notifying the 100 or so gene therapy experimenters using adenovirus vectors, which are made using a disarmed version of the virus that causes the common cold. (nature.com)
Verma describes vectors as the "Achilles heel" of gene therapy, and says that dose-escalation studies using adenoviruses should be re-examined. (nature.com)
Most gene therapy involves retroviral vectors, but adenoviruses are popular for cancer and cystic fibrosis. (nature.com)
Verma says he thinks vectors like the one used in the OTC trial will soon be abandoned in favour of 'gutless' adenoviruses, retroviruses, AAV or lentivectors. (nature.com)
Replication defective adenovirus vectors with low sero-prevalence and low T-cell reactivity in the relevant human populations. (nih.gov)
Schematic of the adenovirus genome and adenovirus-based vectors. (genemedi.com)
pAd1129-01 is a shuttle plasmid designed for constructing E3-deleted and bipartite (dicistronic) recombinant adenovirus vectors. (od260.com)

Recombinant adenovirus type 5 (rAd) has been used as a vaccine platform against many infectious diseases and has been shown to be an effective vaccine vector. (mdpi.com)
A DNA vaccine expressing full- isolated from the 1997 human outbreak in Hong Kong ( 3 ). (cdc.gov)
The ChAdOx1-S [recombinant] vaccine uses a DNA adenovirus vector to elicit antibodies to the SARS-CoV-2 spike protein. (who.int)
Furthermore, to facilitate the transfer of the functional interfering RNA sequences to infected animals, we have constructed a recombinant DNA viral vector commonly used as a vaccine. (europa.eu)
Two among the various possibilities produced in India are inactivated virus vaccine and DNA plasmid vaccine. (netindian.in)
Two (the Moderna vaccine and the Pfizer/BioNTech vaccine) are based on mRNA technology, whereas the other two (produced by Johnson & Johnson and AstraZeneca) are based on a double-stranded DNA recombinant viral vector. (theepochtimes.com)
Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vector. (emmes.com)
Here, we report the safety, tolerability, and immunogenicity of a replication-defective recombinant adenovirus serotype 5 (rAd5) vector HIV-1 candidate vaccine. (emmes.com)
This multiclade rAd5 HIV-1 vaccine is now being evaluated in combination with a multiclade HIV-1 DNA plasmid vaccine. (emmes.com)
One live vaccine licensed for use in the United States (oral adenovirus vaccine) is not attenuated. (cdc.gov)
rVSV-ZEBOV is a live-attenuated recombinant viral vaccine. (msdmanuals.com)
Ad26.ZEBOV is a recombinant DNA vaccine based on adenovirus type 26 (Ad26). (msdmanuals.com)
MVA-BN-Filo is a recombinant DNA vaccine based on modified vaccinia Ankara (MVA). (msdmanuals.com)
A recombinant vesicular stomatitis vaccine vector protected non-human primates in pre- and post-exposure challenge studies. (biomedcentral.com)

Over one hundred vaccines using varied approaches including protein subunit, viral vector, RNA, DNA, inactivated virus, attenuated virus, and virus like particles (VLPs) platforms are in clinical trials. (nature.com)
The generic group of ChAdOx1-S [recombinant] vaccines includes AstraZeneca/AZD1222 and SII/Covishield vaccines. (who.int)
WHO SAGE interim recommendations on the AstraZeneca COVID-19 vaccines refer to a generic group of ChAdOx1-S [recombinant] vaccines against COVID-19. (who.int)
From adenovirus-based live-attenuated virus to recombinant genetic technology, various methods are used to develop several types of vaccines. (netindian.in)
The mRNA vaccines contain only the code for the SARS-CoV-2 envelope spike protein, whereas the DNA-based vaccines both contain an adenovirus viral vector that has been augmented with DNA that codes for the SARS-CoV-2 spike protein. (theepochtimes.com)
The DNA-based vaccines have a certain advantage over the RNA-based vaccines in that they do not have to be stored at deep-freeze temperatures, because double-stranded DNA is much more stable than single-stranded RNA. (theepochtimes.com)
or fractional vaccines such as recombinant vaccines, produced through genetic technologies. (cdc.gov)
Several recently developed non-live vaccines do not contain antigen but employ RNA or DNA to instruct the recipient's own cellular mechanism to generate antigenic material. (cdc.gov)
Topical application of attenuated live vaccines has been more successful, and also the use of vaccinia recombinant viruses expressing foreign paramyxoviral glycoproteins has shown promising results in laboratory animals. (eur.nl)
Live vaccines based on adenovirus types 4 and 7 in oral enteric-coated vaccines, which lead to virus replication in the intestines but not in the respiratory tract have been included in military vaccination programs. (eur.nl)
Little is known of the T cell repertoire these recombinant vaccines induce. (biomedcentral.com)
T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and probably contributed to protection. (biomedcentral.com)
Recombinant adenovirus vaccines should therefore aim at replicating the naturally occurring PPRV immunity. (biomedcentral.com)
DNA vaccines , including DNA delivery device technologies and DNA expressed immune modulators. (nih.gov)
A few vaccines in the UK schedule are made using recombinant DNA technology . (ox.ac.uk)
This is not the case with the currently available EU approved vaccines that target the Spike protein, whether using recombinant technology, adenovirus, or even DNA/RNA. (immunethep.com)

With this adenovirus vector, an active siRNA sequences (N1-PPRV) was transcribed into the infected cells as a short hairpin RNA precursor (shRNA, with approximately 50 nt). (europa.eu)
The silencing capacity of this recombinant viral vector, called Ad-shRNAN1, was firstly verified in vitro, i.e. in PPRV infected cell cultures. (europa.eu)
Gene therapy represents the delivery of therapeutic factors as genetic information encoded on a DNA vector and has also become a potent tool in tissue engineering and regenerative medicine. (genengnews.com)
Their enquiries centre on the adenovirus vector used to deliver potentially therapeutic DNA to the liver. (nature.com)
According to the protocol, researchers at the University of Pennsylvania's Institute for Human Gene Therapy used an 'E1-deleted, E2A-temperature-sensitive' adenovirus vector to infect liver cells with the normal OTC gene, which codes for a urea-cycle enzyme that removes excess nitrogen from the body. (nature.com)
YKL-1, E1B 55 kDa-deleted recombinant adenovirus vector, capable of harboring a transgene casette of up to 4.9 kb, was newly constructed by reintroducing E1A and E1B 19 kDa into E1/E3-deleted adenoviral vector with a homologous recombination in E. coll. (elsevierpure.com)
[ 21 ] reported using a GSD type II mouse model, a new mode of gene therapy using muscle as a secretary organ, and an adenovirus vector encoding AdGAA. (medscape.com)
They injected adenovirus vector encoding AdGAA in the gastrocnemius of neonates and detected a strong expression of GAA in the injected muscle, secretion into plasma, and uptake by the peripheral skeletal muscle and the heart. (medscape.com)

A combination of DNA vaccination and a recombinant adenovirus serotype 5 boost resulted in cross-protective immunity in non-human primates. (biomedcentral.com)
Top panel: a simplified map of the adenovirus serotype 5 genome showing the early genes (E1-E4) and the region from which the major late transcript is produced. (genemedi.com)

ViVU aims to provide the research community with lentivirus, adenovirus (Ad) and adeno-associated virus (AAV) for basic and preclinical studies. (cnic.es)
468.00 : SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents. (signagen.com)
In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)

Vaccination with M2, explored include peptide-carrier conjugates ( 6 ), bac- with key sequences represented, may provide broad pro- ulovirus-expressed M2 ( 7 ), fusion proteins ( 8,9 ), multiple tection against influenza A. antigenic peptides ( 10 ), and M DNA constructs that poten- tially express M2 ( 11,12 ). (cdc.gov)
The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
Compared to the delivery of recombinant proteins, gene therapy provides several advantages such as lower manufacturing costs and increased bioactivity of the produced proteins due to host-specific posttranslational modifications and correct folding of the locally produced growth factors. (genengnews.com)
1 Opposed to this, a burst effect that takes place after application of recombinant proteins often results in unwanted adverse effects. (genengnews.com)
Immunization strategies against this morbillivirus using recombinant adenoviruses expressing PPRV-F or -H proteins can protect PPRV-challenged animals and permit differentiation of infected from vaccinated animals. (biomedcentral.com)
Recombinant HIV protein including HIV envelopes, envelope derivatives, Virus Like Particles, as well as formulations of HIV proteins with immune enhancers such as adjuvants and immune modulators. (nih.gov)
Then the endosome acidifies, resulting in disassociation of capsid proteins and transportation of viral DNA into nucleus. (genemedi.com)
Animals were mucosally primed twice with replicating adenovirus type 5 host range mutant (Ad5hr)-SIV recombinants and systemically boosted with ALVAC-SIV M766 Gag/Pro/gp120-TM and SIV M766&CG7V gD-gp120 proteins formulated in alum hydroxide (ALVAC/Env) or DNA encoding SIVenv/ SIVGag/rhesus interleukin 12 (IL-12) plus SIV M766&CG7V gD-gp120 proteins formulated in alum phosphate (DNA&Env). (edu.pe)

The adenovirus genome is a linear, double stranded DNA of approximately 36 Kb. (cnic.es)
To generate recombinant adenovirus, that can be safely used as a genetic tool, its genome has two genes deleted: E1 and E3. (cnic.es)
These viruses contain a linear, single-stranded DNA genome (approximately 5.1 kb in length). (biomedcentral.com)
It is a medium sized (90-100nm) and non-enveloped virus with an icosahedral nucleocapsid containing a 36kb double stranded DNA genome (Fig. 7A). (genemedi.com)
Without integration into host genome, adenovirus genome remains in an episomal state, which guarantees the low risk of mutation (Fig. 8). (genemedi.com)
When you construct a recombinant adenovirus genome in E. coli using cosmid technology, you are almost sure that every colony carries a full-size genome, and not a smaller plasmid that lost chuncks of the adenovirus genome by DNA recombination. (od260.com)
Both of them are flanking both ends of the recombinant adenovirus genome contained in your cosmid. (od260.com)
Because the PacI site is immediately flanking the start of the adenovirus genome, the DNA ends generated by PacI resemble the most the ends obtained from deproteinised virion DNA and might therefore be more efficient in promoting virus replication. (od260.com)

Mice primed with M2-DNA channel protein. (cdc.gov)
Moreover, the long-term systemic delivery of a recombinant protein is an expensive, painful experience for patients and is cumbersome for medical staff. (biomedcentral.com)
But a disadvantage is that those who have been exposed to natural forms of the adenovirus have antibodies to the virus that will likely block the synthesis of the spike protein, and therefore not afford protection against SARS-CoV-2. (theepochtimes.com)
Infection of a panel of human melanoma cell lines by AdCMVMAGE-1, a novel recombinant adenovirus which incorporates the full-length MAGE-1 cDNA, was shown to induce production of high levels of MAGE-1 protein. (illinois.edu)
A 1.9 kb XbaI fragment encoding gp19K membrane protein and the adenovirus "death" protein (ADP, 11.6K) was deleted, and replaced with a multiple cloning site. (od260.com)

The best PSP was selected to construct a prostate-specific oncolytic adenovirus (CRAD) by controlling the adenoviral E1 region. (hindawi.com)
The rationale led to development of the rAd-IFNα recombinant adenovirus with the syn 3 excipient to enhance adenoviral transduction of NMIBC cells. (medpagetoday.com)
The in-house adenovirus assay is a sensitive and reliable assay with lower cost for the detection and quantification of adenoviral DNA when compared to the RealStar® Adenovirus PCR Kit. (biomedcentral.com)

Here, we describe the characterization of an HAdV-1 recombinant (QH-1665/2018) isolated from an infant aged one month with influenza-like illness in Qinghai Province in 2018. (biomedcentral.com)
Characterization of a Novel HIV-1 Circulating Recombinant Form (CRF80_0107) among Men Who Have Sex with Men in China. (cdc.gov)

This vaccination induced challenge infection, DNA vaccination with NP and M antibodies that cross-reacted with divergent M2 peptide achieved limited protection against an H5N1 virus strain from an H5N1 subtype. (cdc.gov)

The idea behind this study is to place the Ad5 E1 region in cis complementation (i.e., use E1 as a transgene) back into an E1-deleted, replication-defective adenovirus under the control of a prostate-specific promoter (PSP). (hindawi.com)

Xenomycins are antimicrobial and antifungal agents that target basic microbial transcription and replication by binding the DNA of pathogens. (wikipedia.org)
As in the case of other viral respiratory pathogens, community outbreaks of adenovirus infections may occur, especially with respiratory tract infections and keratoconjunctivitis. (biomedcentral.com)

DNA resources as well as strains [link] are available at "Yeast, National BioResource Project", Yeast Genetic Resource Center (YGRC), Graduate School of Science, Osaka City University. (riken.jp)
Loss of DNA on chromosome 22 has been shown in 40% of meningiomas, and, in cytogenetic studies, genetic abnormalities at the level of chromosome 22 are seen frequently in meningiomas with loss of a copy of chromosome 22 as the most commonly reported abnormality. (medscape.com)

Adenovirus infections do not normally cause hepatitis in healthy children suggesting that a completely new type of adenovirus might have been introduced into the human population. (bmj.com)
Gene therapy using a recombinant adenovirus (Ad) encoding secretory human endostatin (Ad-Endo) has been demonstrated to be a promising antiangiogenesis and antitumor strategy of in animal models and clinical trials. (biomedcentral.com)
In Ebolavirus -infected cynomolgus macaques, use of human recombinant interferon alfa-2b in conjunction with hyperimmune equine immunoglobulin G (IgG) delayed but did not prevent death. (medscape.com)
A recombinant nonreplicating human adenovirus type 5, Ad5HCMVsp1lacZ, expressing the lacZ gene under control of the human cytomegalovirus immediate early promoter, was used to assess the effect of heat-shock (HS) on DNA repair of a UV-damaged reporter gene. (mcmaster.ca)
Human adenoviruses (HAdVs) are prevalent in patients with respiratory infections, in which recombination has important implications for viral detection and pathogenicity. (biomedcentral.com)
Currently, human adenoviruses (HAdVs) are classified into seven groups (HAdV-A to HAdV-G), including 52 serotypes and 90 genotypes [ 4 ]. (biomedcentral.com)
Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [42]. (genemedi.com)
Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. (biomedcentral.com)

We also show that recombinant adenovirus vaccination induced T cell responses to the same epitopes, and led to memory T cell differentiation. (biomedcentral.com)

The Recombination Detection Program (RDP) package Beta 4.100 was used for identification of recombinant sequences in default mode. (biomedcentral.com)
The adenovirus sequences are flanked by two SfiI sites, which generate non-symmetrical sticky ends suitable for directional cloning with the other AdenoQuick2.0 plasmids (pAd1127, pAd1128, pAd1130, and their derivatives). (od260.com)

Together, our data emphasize the utility of adenoviruses as vaccination vehicles and highlight the potential efficacy of this approach for the treatment of melanoma. (illinois.edu)

A major problem of adenoviruses is that even inactivated versions can stimulate an immune response. (nature.com)

Recombination analysis based on the RDP4 package and SimPlot software showed that QH-1665/2018 was a recombinant involving HAdV-1, HAdV-2 and HAdV-5, which was then re-confirmed by phylogenetic analysis. (biomedcentral.com)
This information would enhance the understanding of recombination of HAdV-C, and might assist with effective prevention and control of respiratory adenovirus infection in Qinghai Province. (biomedcentral.com)

To introduce exogenous DNA into cells in vitro and in vivo , several strategies based on viral or nonviral approaches are available. (genengnews.com)
The YKL-1 and DNA damaging agent, camptothecin effectively induced p53 in H460 and HepG2 as well as in normal cells. (elsevierpure.com)
HSER in normal and XP-C cell lines, but not CS-A, XP-A, XP-D or XP-G cells, suggests that HS treatment can enhance the repair of UV-damaged DNA through an enhancement of transcription coupled repair (TCR) or a mechanism which involves the TCR pathway. (mcmaster.ca)
Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. (riken.jp)
Shen, Y.H., Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice. (riken.jp)
QH-1665/2018 caused adenovirus-like CPE of HEp-2 cells, and the cultures underwent three passages to obtain high-titer stocks. (biomedcentral.com)
Once packaged into a E1-complementing cell line, which provides the E1 products in trans, such as QBI 293A Cells, recombinant viral will be easily propagated. (genemedi.com)
In practice however, no difference in the time needed to recover the virus after DNA transfection into 293 cells is observed between both settings. (od260.com)
Virus plaques can appear as early as 4 days after transfecting PacI- or SwaI-digested DNA into 293 cells. (od260.com)

These data validate the use of recombinant adenovirus expressing PPRV genes as DIVA strategies to control this highly contagious disease. (biomedcentral.com)

E1 gene is implicated on replication of virus and is supplied by the adenovirus packaging cell line 293A. (cnic.es)

For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR), which mainly determines the viral tropism [44]. (genemedi.com)
Infection process of adenovirus. (genemedi.com)

To overcome this obstacle, conditional oncolytic viruses (such as conditional replication adenovirus (CRAD)) are developed to specifically target prostate without (or with minimal) systemic toxicity due to viral self-replication. (hindawi.com)

The analytical sensitivity, specificity, linearity, precision and accuracy of an in-house adenovirus quantitative polymerase chain reaction assay were evaluated against the RealStar® Adenovirus PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), using 122 clinical specimens and 18 proficiency testing samples. (biomedcentral.com)

La información más reciente sobre el nuevo Coronavirus de 2019, incluidas las clínicas de vacunación para niños de 6 meses en adelante. (stanfordchildrens.org)
Contexte: Pour contrôler la propagation de la maladie à coronavirus 19 (COVID-19) causée par le syndrome respiratoire aigu sévère coronavirus-2 (SRAS-CoV-2), il est nécessaire d'identifier et d'isoler de manière adéquate les patients infectieux, en particulier sur le lieu de travail. (bvsalud.org)

The Qinghai adenovirus strain(QH-1665/2018) was isolated from an outpatient, whom was one month old and diagnosed with an influenza-like illness at Women's and Children's Hospital of Qinghai Province on November 26, 2018. (biomedcentral.com)

Fukuda K., E1A, E1B double-restricted replicative adenovirus at low dose greatly augments tumor-specific suicide gene therapy for gallbladder cancer. (riken.jp)

DNA constructs that express conserved influenza A nucle- on conserved antigens provide broader protection. (cdc.gov)
M2 DNA fragments and subcloned into VR-1012, offer broad protection against multiple influenza subtypes, obtained under material transfer agreement from Vical, including H5N1. (cdc.gov)

The recombinant monoclonal antibody combination, atoltivimab/maftivimab/odesivimab (Inmazeb), was approved by the FDA in October 2020 and is the first approved treatment for Zaire ebolavirus. (medscape.com)

To prevent infected cell lysis, recombinant replication deficiency virus has been developed as a gene delivery tool to replace wild-type adenovirus (recombinant AdV in "application" part). (genemedi.com)

A recombinant adenovirus expressing beta-galactosidase (lacZ) under control of CAG promoter. (riken.jp)

Amplification: starting from stocks of adenovirus. (cnic.es)

Adenovirus transcription is a two-phase event, early and late, occurring before and after viral DNA replication, respectively (Figure. (genemedi.com)

Adenovirus infections might cause the acute hepatitis in children observed. (bmj.com)

Host cell reactivation (HCR) of beta-galactosidase (beta-gal) activity for UV-irradiated Ad5HCMVsp1lacZ was used as an indicator of DNA repair in the transcribed strand of an active gene. (mcmaster.ca)

The Ad DNA copy number was determined by real-time PCR, and gene expression was assessed by ELISA, Western blotting or immunohistochemistry. (biomedcentral.com)

Anatomy23

Organisms42

Diseases9

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes63

Disciplines and Occupations1

Information Science4

Health Care3

Vectors14

Vaccine14

Vaccines16

Vector8

Serotype2

Lentivirus3

Proteins8

Genome8

Protein5

Adenoviral3

Characterization2

Antibodies1

Replication-defective1

Pathogens2

Genetic2

Human8

Vaccination induced1

Sequences2

Efficacy1

Immune1

HAdV2

Cells9

Genes1

293A1

Infection2

Viruses1

Polymerase1

Coronavirus2

20181

Tumor-specific1

Influenza2

Ebolavirus1

Type1

LacZ1

Amplification1

Transcription1

Hepatitis1

Cell1

Expression1