Dna amplification fingerprinting. Medical search. Frequent questions

The scientist performing RAPD creates several arbitrary, short primers (10-12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. (wikipedia.org)
No knowledge of the DNA sequence of the targeted genome is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. (wikipedia.org)
Unlike traditional PCR analysis, RAPD does not require any specific knowledge of the DNA sequence of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions that are complementary to the primers' sequence. (wikipedia.org)
The only limiting feature of the application of these markers is the need for prior sequence information for developing primers in locus-specific PCR amplification. (tubitak.gov.tr)
LAMP primers were designed based on the Sm1-7 and Dral repeated sequences of the corresponding schistosomes, and amplification by LAMP of these 121-basepair highly abundant sequences provided a detection sensitivity of 0.1 fg of genomic DNA. (huji.ac.il)
Framents are generated from whole genomic DNA using primers with a length of about 10 nucleotides. (sequentix.de)
These primers bind and amplify DNA regions that are complementary to them and that are close enough to each other. (sequentix.de)
To identify DNA-based genetic polymorphism for constructing a genetic linkage map of catfish, we tested 100 random amplification of polymorphic DNA (RAPD) primers for their utility in identifying genetic polymorphism in catfish. (syr.edu)

Random amplified polymorphic DNA (RAPD), pronounced "rapid", is a type of polymerase chain reaction (PCR), but the segments of DNA that are amplified are random. (wikipedia.org)
RAPD markers are decamer (10 nucleotides long) DNA fragments from PCR amplification of random segments of genomic DNA with a single primer of arbitrary nucleotide sequence and which are able to differentiate between genetically distinct individuals, although not necessarily in a reproducible way. (wikipedia.org)
RAPD requires only one primer for amplification. (wikipedia.org)
Nearly all RAPD markers are dominant, i.e. it is not possible to distinguish whether a DNA segment is amplified from a locus that is heterozygous (1 copy) or homozygous (2 copies). (wikipedia.org)
Codominant RAPD markers, observed as different-sized DNA segments amplified from the same locus, are detected only rarely. (wikipedia.org)
Random amplification of polymorphic DNA (the RAPD assay) was used to amplify fragments of DNA (via the polymerase chain reaction) to obtain fingerprints of parental colonies and larval offspring of the hermaphroditic freshwater bryozoan Cristatella mucedo. (elsevierpure.com)
The methods considered to-date to deliver the best result for differentiation of strains are Rapid Amplification of Polymorphic DNA (RAPD), Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) analysis. (uni-muenchen.de)

Each SSR locus can easily be amplified by using a polymerase chain reaction (PCR) knowing the DNA sequence flanking the repeat region specifically. (tubitak.gov.tr)
The Taq DNA Polymerase is intended for molecular biology applications. (qiagen.com)
Taq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer that minimizes the need for optimization of PCR parameters, as well as Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g. (qiagen.com)
1.Taq DNA Polymerase is one of the most commonly used DNA polymerases. (elisatest-kits.com)
Using a highly advanced method of DNA replication and amplification known as polymerase chain reaction, we can map a horse's DNA profile. (gtldna.co.uk)
A DNA fragment analysis technique where the complete genomic DNA is digested with ristriction enzymes and a part of the generated fragments is amplified using polymerase chain reaction (PCR). (sequentix.de)
Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
PCR (polymerase chain reaction) technique has achieved increased importance for post-mortem DNA analysis in forensic cases 8 because of the millions of copies amplified from one specific sequence of DNA. (bvsalud.org)
Culture is the most common diagnostic test for gonorrhea, followed by the deoxyribonucleic acid (DNA) probe and then the polymerase chain reaction (PCR) assay and ligand chain reaction (LCR). (medscape.com)

Abstract A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. (ulster.ac.uk)
This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets. (ulster.ac.uk)
After ultracentrifugation, 13C-labelled DNA, containing genomic DNA of these Methylocystis spp. (lancs.ac.uk)
This combination of DNA stable-isotope probing, MDA and metagenomics provided access to genomic information of a relatively large DNA fragment of these thus far uncultivated, predominant and active methanotrophs in peatland soil. (lancs.ac.uk)
These techniques use DNA fragments of known length that are usually generated by PCR amplification of a genomic region (e.g. a gene). (sequentix.de)
Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. (bvsalud.org)

The proposed system uses the isothermal amplification of DNA in a rapid assay format, which returns results in as little as 30 min from sampling. (tees.ac.uk)
Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for the Detection of Treponema pallidum DNA in the Peripheral Blood of Secondary Syphilis Patients. (cdc.gov)

DNA fingerprinting of Paecilomyces strains of potential use for the biological control of pests. (uchile.cl)
DNA fingerprinting has identified 5 different strains of M. tb from elephants located throughout the U.S. Results of nucleic acid amplification tests were positive for 2 elephants where M. tb was isolated and 14 elephants where mycobacteria were not isolated. (usda.gov)
Results obtained from Restriction fragment length polymorphism typing show that the mahority of circulating Mycobacterium tuberculosis strains in Sri Lanka belong to a limited number of families, but the degree of IS6160 DNA polymorphism among strains were high. (who.int)
Of the 20 strains isolated from prisoners, none of the strains displayed identical fingerprints. (who.int)

It involves species-specific primer amplification, random amplified polymorphic DNA, arbitrarily primed, DNA amplification fingerprinting, inter simple sequence repeat (ISSR) etc. (fmtmagazine.in)

We have now developed loop-mediated isothermal amplification (LAMP) assays for identifying Schistosoma mansoni and S. haematobium to facilitate large-scale evaluation of post-intervention transmission potential. (huji.ac.il)
We designed these to run any isothermal amplification method that employs target detection by fluorescence measurement. (technosysindia.in)

Simple Sequence Repeat (SSR) DNA markers or Microsatellites are di-, tri-, tetra-nucleotide tandem repeats containing loci of eukaryotic genomes. (tubitak.gov.tr)
This limitation is alleviated for the economically important species and the ones closely related, since primer sequences of the SSR DNA markers and the amplification conditions are available in the literature. (tubitak.gov.tr)
However, when the reported PCR amplification conditions were applied, with the 2 wheat SSR markers, we found that contaminating bands appeared on the gels. (tubitak.gov.tr)
Of the 343 randomly selected markers, 82.2% showed amplification validation, with up to 35.5% polymorphism. (biomedcentral.com)
Different types of markers have been developed and employed for diversity analysis, DNA finger-printing, trait mapping and genomics-assisted breeding (GAB). (biomedcentral.com)
The profile we provide includes the STR marker panel plus additional markers for an optimized panel of 23 DNA markers. (gtldna.co.uk)
A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)

Because it relies on a large, intact DNA template sequence, it has some limitations in the use of degraded DNA samples. (wikipedia.org)
After amplification with PCR, samples are loaded into a gel (either agarose or polyacrylamide) for gel electrophoresis. (wikipedia.org)
The identification of samples at a crime scene which require forensic DNA typing has been the focus of recent research interest. (tees.ac.uk)
The objective of the study was to develop a rapid method to detect Mycobacterium tuberculosis from clinical samples and the applicability of DNA amplification techniques to a developing country like Sri Lanka. (who.int)
For the DNA extraction and purification from the clinical samples two methods, the standard phenol extraction procedure desctibed by Sambrook etal (1982) and the guanidinium thiocyanate method described by Boom et al(1990) were used. (who.int)
After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)
Blood samples were subjected to DNA extraction and PCR was performed to estimate the prevalence of E. canis infection. (bvsalud.org)

Results of DNA fingerprinting of the isolates from the 4 elephants and the handler showed that they were infected with the same strain of M. tb. (usda.gov)

Assessment Of The Genetic Diversity Of Xylella Fastidiosa Isolated From Citrus In Brazil By Pcr-rflp Of The 16s Rdna And 16s-23s Intergenic Spacer And Rep-pcr Fingerprinting . (uchile.cl)
Direct genetic study of this question using routine techniques has been limited by both the amount of material required and the detection of adequate DNA polymorphisms. (elsevierpure.com)
In recent months, however, the prosecution has suggested that it may use low-template DNA analysis to tie Mr. Hillary to Garrett's murder, utilizing sophisticated algorithms to determine statistical probabilities by analyzing genetic data that older methods could not interpret. (blogspot.com)
A horse DNA profile is a personal and unique form of genetic identification. (gtldna.co.uk)
Our DNA profiling service will provide you with your 23 genetic marker blue print extracted in an ISO 17025 accredited laboratory. (gtldna.co.uk)
Because a DNA profile offers proof of genetic identification that is incontrovertible and indisputable it can be used in a number of cases. (gtldna.co.uk)

Her research has spanned several areas including gunshot residue analysis, whole genome amplification, DNA admixture analysis, and familial DNA analysis. (worldsciencefestival.com)
With the 30th Leibniz Conference, the Leibniz Institute for Interdisciplinary Studies (LIFIS e.V.) is honouring the 70th anniversary of the discovery of DNA, the central molecule of genome research. (leibniz-institut.de)
As in the previous conference "2013-Year of DNA" , the selected speakers for this one-day conference represent the current state of the different fields of today's genome research and show the most important successes of the translation of their results for the prevention and cure of diseases before and during the global pandemic. (leibniz-institut.de)
It focuses on amplification of micro-satellite regions/DNA in the genome, yield a unique pattern for an individual and helps in authentication and adulteration of spices. (fmtmagazine.in)

The determination of the sequence of deoxynucleotides in DNA fragments. (croptrust.org)
The pulse field gel electrophoresis is used to separate large size DNA fragments, e.g. chromosomes. (sequentix.de)
The SINGLE-USE Brush is DNA-Free and following manufacturing is packaged in a sealed, sterile envelope and treated with Ethylene Oxide to degrade any DNA contamination to such small fragments that no human profile can be generated with PCR amplification methods. (shopevident.com)

DNA profiling (sometimes referred to as DNA fingerprinting) is a type of analysis which is very much analogous to the tests used by forensic teams whereby scientists analyse a DNA pattern that has a unique sequence. (gtldna.co.uk)
Thus, it is of fundamental importance that dentists who act in Forensic Dentistry become familiar with the technological aspects of DNA analysis, as well as gradually incorporate this methodology into their practices 17 . (bvsalud.org)

This makes the method popular for comparing the DNA of biological systems that have not had the attention of the scientific community, or in a system in which relatively few DNA sequences are compared (it is not suitable for forming a cDNA databank). (wikipedia.org)
In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)
We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)

PCR is a process used to amplify small bits of DNA so that it can be used for DNA fingerprinting and other important projects. (thingsaregood.com)

The lectures will give an overview of the different forms and the importance of DNA and RNA, modern trends in the analysis of gene data, the use of the results for biology, medicine, social and engineering sciences. (leibniz-institut.de)

Therefore, if a mutation has occurred in the template DNA at the site that was previously complementary to the primer, a PCR product will not be produced, resulting in a different pattern of amplified DNA segments on the gel. (wikipedia.org)

Dreaming of a career in a specialised field like crime scene investigation, DNA analysis, or toxicology? (ntu.ac.uk)
were further confirmed by DNA stable-isotope probing analysis of a sample taken from the Moor House peatland (England). (lancs.ac.uk)
Potential bias of MDA was detected by fingerprint analysis of 16S rRNA using denaturing gradient gel electrophoresis for low-template amplification (0.01 ng template). (lancs.ac.uk)
In order to establish the effectiveness of tryptophan fingerprinting for strain identification, we compared the graphic analysis of tryptophan-labelled bands in the gel images with MLST results. (uni-muenchen.de)
dc.title: PCR based detection techniques and DNA finger printing by restriction fragment analysis of mycobacterium tuberculosis dc.contributor.author: MAGANAARACHCHI, DN dc.description.abstract: In Sri Lanka definitive diagnosis of tuberculosis depends on the culture of mycobacteria, but the slow growth of the organism delays the diagnosis. (who.int)
With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. (bvsalud.org)

This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)

As these repetitive motifs are quite variable due to slippage effects during DNA replication, they are useful for a comparison of closely related organisms, e.g. for paternity tests. (sequentix.de)

Together they form a unique fingerprint. (tau.ac.il)

The sensitivity of the PCR method is largely dependent on the efficiency of the DNA extraction procedure which, could probably be improved by modifications of the lysis technique. (who.int)

A high throughput screening technique based on the hybridization between oligonucleotide probes and either DNA or mRNA carried out on miniaturized reaction surfaces. (croptrust.org)

Due to this resilience against environmental changes (incineration, immersion, traumas, mutilation and decomposition), teeth represent an excellent source of DNA, when the other biological sources have been lost 20,29,30 . (bvsalud.org)

We also tested each locus for amplification and polymorphism on 11 other scarine labrid species and one labrid species. (edu.sa)

PCR is an enzymatic reaction, therefore, the quality and concentration of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. (wikipedia.org)

New York Times sets up a DNA hearing set to begin Monday 26, July, is described as "shaping up as an important legal test of a cutting-edge method of teasing DNA evidence out of microscopic amounts of biological matter. (blogspot.com)
GIST: "A long-simmering murder case in northern New York connected to the strangulation of a 12-year-old boy is shaping up as an important legal test of a cutting-edge method of teasing DNA evidence out of microscopic amounts of biological matter. (blogspot.com)

Metagenomic binning and association of plasmids with bacterial host genomes using DNA methylation. (cdc.gov)

Its resolving power is much lower than targeted, species-specific DNA comparison methods, such as short tandem repeats. (wikipedia.org)
Amplification success ranged from zero to ten loci per species. (edu.sa)

Since the Taq enzyme has no 3' to 5' exonuclease activity, it acts as A nucleotide transferase during DNA polymerization, resulting in a 3'-dA overhangs on the 3' end of the PCR product, i.e., a 3' sticky end with an A. (elisatest-kits.com)

was separated from 12C DNA and subjected to multiple displacement amplification (MDA) to generate sufficient DNA for the preparation of a fosmid metagenomic library. (lancs.ac.uk)

What kind of DNA fingerprints can GelQuest analyze? (sequentix.de)

The biotechnological methods adopted were the Polymerized Chain Reaction (PCR) method, hybridization method, and DNA sequencing method. (fmtmagazine.in)

Teeth have in their composition the most resistant tissue in the human body - dental enamel - which provides high resistance against adverse conditions that can degrade DNA in the whole dental structure 14 . (bvsalud.org)

STORY: "Potsdam Boys murder case: Potsdam Boy's Murder Case May Hinge on Minuscule DNA Sample From Fingernail," by reporter Jesse McKinley, published by the New York Times on July 25, 2016. (blogspot.com)

Plasmid DNA, when amplified by PCR, provides products that represent individual DNA profiles. (isthhongkong.com)
If the DNA profiles match, it is your horse. (gtldna.co.uk)
DNA profiles are also used if you are a horse breeder and wish to keep records of dames and sires. (gtldna.co.uk)
Having DNA profiles on record makes equine parentage testing quicker. (gtldna.co.uk)

The SINGLE-USE Fiberglass Brush - DNA-Free features Ethylene Oxide (EtO) Sterilization and is designed for a single application to reduce possible cross contamination. (shopevident.com)

The differing sizes created through random amplification will separate along the gel in a repeatable manner depending on the sample source. (wikipedia.org)

Anatomy20

Organisms19

Diseases12

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes76

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care5

Primers8

RAPD7

Polymerase9

Genomic6

Assay2

Strains4

Polymorphic1

Isothermal amplification2

Markers7

Samples7

Isolates1

Genetic6

Genome4

Fragments3

Forensic2

Sequences3

Amplify1

Gene1

Primer1

Analysis6

Sequence2

Replication1

Unique1

Extraction1

Oligonucleotide1

Decomposition1

Locus1

Concentrations1

Biological2

Bacterial1

Species2

Nucleotide1

Generate1

Analyze1

Method1

Degrade1

Reporter1

Profiles4

Contamination1

Random1